The renomedullary interstitial cells (RMIC) of rat were investigated using the method of HCl-Tween removing extracellular material and scanning electron microscope. According to the shape and distribution, the RMIC may be divided into 3 groups. However, most of RMIC belong to the first group.The cell of the first group is fibroblast-like, which has an irregular cylindrical body (about 2-6 ?m in short diameter and 5-12 ?m in long diameter) and several processes from both ends of the body. The processes from one end are distributed on the wall of the renal tubule (Henle's loop or collecting tubule); and those from the other end on the blood vessel, sometimes, with the vascular pedicles formed by the expanding of the process end. The long axis of the cells of the first group is perpendicular to the tubules and blood vessels, so the cells are bridged between them.The second group cell has a round body without process and is located at the body of the first group cell.The third group cell is star-like, which has a large body (about 5-8 ?m in diameter) and a few small processes. They are randomly distributed, and sometimes, arranged as a line along the blood vessel.

Objective To evaluate ANA and ANAs as a indacation of systemic sclerosis diagnostic.Method ANAs were tested in 58 patients with SSc and 20 healthy controls by indirect immunofluorescence (ⅡF)and immunoblot(IB).Results ⅡF-ANA results indicated that the positive of ANAs were 98.3%(57/58)in SSc,and ANA pattern were mainly homogeneous-nucleolar,IB-ENA results indicated that the antinuclear antibodies were mainly anti-Scl-70.anti-SSA and anti-nRNP in SSc.The positive of them were 63.8%、31.O%and 5.2%.Conclusions It suggested that the patients might have other autoimmune diseases or the dangerous of other autoimmune diseases,and the IB kits of euroimmun got better positives and specificities to ANAs,the method could provide more helps to diagnosis of SSc.

Carcinosarcoma ; Female ; Humans ; Maxillary Sinus Neoplasms ; Middle Aged

By using replica cast SEM method, the vascular architecture of the dental pulp of rat incisors was studied. The characteristics of the vascular architecture are as follows:1. The axis of the dental pulp is occupied by the central vascular bundle, which consists of 10 to 20 arterioles and 8 to 12 venules.2. The arterioles (30-50?m in diameter) and the venules (50-80?m in diameter) located in the central bundles are arranged parallely in a relatively long distance as a countercurrent system. The longest distance is about 12 mm.3. Each arteriole of the central vascular bundle supplies an independent area of the dentin of the tooth. The territory of an arteriole is about 700 to 1400 ?m in length.4. The arteriole of the central bundle breaks up into capillaries (4～6?m in diameter) which are mainly distributed over the odontblast area and form a complex 3-dimensional plexus.

Objective To investigate the histomorphology,cellular biocompatibility in vitro and tensile force resistance property of fibrin-binding amniotic membrane(FBAM)and to explore its feasibility and superiority using as ocular surface graft.Methods After observing the morphology of FBAM under light microscope and scanning electron microscope,we did primary culture of corneal epithelial cells,taking monolayer fibrin-binding amniotic membrane as carrier,then observed cell growth overlay area.The elongation nature of FBAM was tested on electronic universal testing machine by calculating elastic modulus,using bilayer amniotic membrane(BAM)and monolayer amniotic membrane(MAM)as controls.Results The fresh FBAM was integrated as a whole,with structurally complete fibrin and amniotic membrane as well as tight binding of fibrin to amniotic membrane.The reserved FBAM was structurally complete,brittler than the fresh FBAM,while amniotic membrane still bound tightly to the fibrin,but under microscope the fiber reticular formation was fairly fuzzy.Single layer of epithelial cells was formed on FBAM after about seven-day culture in vitro.Disparity of cell growth overlay area was significant(P

The microvascutature of the adrenal medulla in the rats and mice were studied by scanning electron microscopy of vascular replicas.There are portal venules between the adrenal cortex and medulla,which are the side branches of the collecting venules of the adrenal cortex and break into capillaries at the adrenal medulla.The adrenal medulla in mice is mainly supplied by these portal venules,and in a rat adrenal medulla,there are also 5—8 medullar arterioles besides the portal venules.Ring-like constrictions appear to be the characters of sphincter at the ending parts of the portal venules,the branches of the medullar arterioles,the collecting venules,and the medullar venules which drain the medullar capillaries as well.The authors believe that the adrenal cortex controls the medulla by the way of the portal venules.

Objective:To assess whether familial occurrence has an influence on the morphological characteristics of patients with nonsyndromic cleft lip and/or palate (NsCL/P).Methods:A case-control analysis was performed on the morphological characteristics of familial group and sporadic group,using medical records of 1967 patients with NsCL/P treated in the Affiliated Stomatological Hospital of Nanchang University from 2002 to 2014.Results:164 (8.34％) cases presented a positive history of cleft in their families.The cleft types,the positive familial rate of cleft lip only (CLO),cleft lip and alveolar ridge(CLA),cleft lip and palate (CLP) and cleft palate only (CPO) were 8.11％,8.54％,6.19％ and 9.65％ respectively.A positive family history of NsCL/P was associated with 0.66 times risk of CPO (P =0.036,OR =0.66,95％ CI 0.44-0.98) compared to those of CLO,CLA and CLP.In familial group of CLP,the lateral incidence of male patients was different from that of female patients (P ＜ 0.001).There was no significant difference between familial group and sporadic group on birth weights,parental child-bearing age and clinical manifestations of patients.Conclusion:Familial occurrence might have an influence on cleft type,laterality and gender of the patients with NSCL/P.

ABSTRACT:Objective To validate that relaxin can resist hepatic fibrosis at the cellular level and explore its molecular mechanism in order to provide experimental basis for the treatment of liver cirrhosis.Methods Cultured HSC-T6s were treated with different concentrations (20,50 and 100 ng/mL)of recombinant human relaxin-2 (RLX-2).The proliferation of HSC-T6 was measured by MTT colorimetric assay.The content of type Ⅰcollagen in the cell culture supernatant of each group was detected by ELISA at 48 h of drug intervention;RT-PCR was used to detect the mRNA expressions of CTGF and TGF-β1 in HSC-T6 at 48 h of drug intervention.Results RLX-2 inhibited the proliferation of HSC and reduced type Ⅰ collagen content of HSC cells.It also inhibited the CTGF mRNA expression of HSC,but did not have a significant effect on the expression of TGF-β1 mRNA. Conclusion In the experiment of culturing HSC-T6 in vitro,RLX-2 may play a role in rat liver fibrosis by inhibiting cell proliferation and type Ⅰ collagen and CTGF mRNA expressions.

Objective To explore the patterns of motor and linguistic activation in cortical and its correlations with abnormal gray matter in patients with malformations of cortical development(MCD)and epilepsy.Methods Seven MCD patients with epilepsy(2 patients with focal cortical dysplasia,2 heterotopia,2 schizencephaly,and 1 polymicrogyria)underwent blood-oxygen-level-dependent(BOLD)functional MRI(fMRI)in a 3 T MR scanner when practicing bilateral fingers tapping,toes twisting,verb generation,and picture naming.Functional images were post-processed by using SPM 5 software based on a general linear model(GLM)to generate activations above a uniform threshold with the cluster size (≥30 voxels,P ＜0.001 corrected).The activations were recognized and classified by two experienced neuroradiologists,and then compared with that in abnormal gray matter.Results The clusters and intensities of motor activations were mainly located in the sensormotor cortex(SMC)and premotor area (PMA).In linguistic tasks,activations produced by verb generation were found in language-associated cortical regions and PMA with higher activation in Wemicke area,picture naming significantly in the visual cortex,and language in Broca area.Combination of the two linguistic tasks produced significant clusters and intensities in language cortex.For MCD patients with abnormal cortical abnormalities,motor and language task could produce neuronal activities within normal as well as abnormal cortex regions.In 6 patients who underwent resective surgery,epileptic seizures decreased significantly,and the follow-up images demonstrated no new neurological dysfunctions and cognitive impairments.Conclusions fMRI can visualize neuronal activities in patients with MCD and epilepsy and demonstrate the motor and linguistic activations occurring in normal and abnormal gray matter.It should be cautious for surgery in patient with MCD and epilepsy.

Objective To explore the relation between the infection of Helicobacter pylori (H .pylori)and colorectal tumor.Methods From January 2012 to January 2013,the medical data of 152 patients with colorectal tumor were collected.According to the findings of colonoscopy examination, the patients were divided into the colorectal adenoma group (84 cases)and the colorectal carcinoma group (68 cases).A total of 88 healthy individuals were also enrolled as control.The differences of H .pylori infection were compared among the colorectal adenoma group,the colorectal carcinoma group and the healthy control group.The distribution of H .pylori infection in different age,gender and nation was analyzed in colorectal tumor group.According to the location,maximum diameter,number,type of pedicle and pathological type of adenoma,the colorectal adenoma group was divided into subgroups and the condition of H .pylori infection was compared among the subgroups.Chi-square test was used in the statistical analysis.Results The positive rates of H .pylori infection of colorectal adenoma group, colorectal carcinoma group and healthy control group were 70.2% (59/84),72.1 % (49/68)and 53.4%(47/88),respectively,where the former two groups were both higher than that of the healthy control group,and the differences were statistically significant (χ2 =5 .147 and 5 .637,both P <0.05).Regarding the H .pylori infection,there was no difference in age,gender and ethnicity among 152 patients with colorectal tumor (all P > 0.05 ).Among 84 patients with colorectal adenoma,there was no statistical difference in the positive rate of H .pylori infection in different location,maximum diameter,number, type of pedicle and pathological type of adenoma (all P > 0.05).Conclusion H .pylori may have promoting effects on the genesis and development of colorectal tumor.