1.RENOMEDULLARY INTERSTITIAL CELLS: SCANNING ELECTRON MICROSCOPY STUDY
Acta Anatomica Sinica 1957;0(04):-
The renomedullary interstitial cells (RMIC) of rat were investigated using the method of HCl-Tween removing extracellular material and scanning electron microscope. According to the shape and distribution, the RMIC may be divided into 3 groups. However, most of RMIC belong to the first group.The cell of the first group is fibroblast-like, which has an irregular cylindrical body (about 2-6 ?m in short diameter and 5-12 ?m in long diameter) and several processes from both ends of the body. The processes from one end are distributed on the wall of the renal tubule (Henle's loop or collecting tubule); and those from the other end on the blood vessel, sometimes, with the vascular pedicles formed by the expanding of the process end. The long axis of the cells of the first group is perpendicular to the tubules and blood vessels, so the cells are bridged between them.The second group cell has a round body without process and is located at the body of the first group cell.The third group cell is star-like, which has a large body (about 5-8 ?m in diameter) and a few small processes. They are randomly distributed, and sometimes, arranged as a line along the blood vessel.
3.Histomorphology,cellular biocompatibility and tensile force resistance property of fibrin-binding amniotic membrane in vitro
Journal of Third Military Medical University 1983;0(04):-
Objective To investigate the histomorphology,cellular biocompatibility in vitro and tensile force resistance property of fibrin-binding amniotic membrane(FBAM)and to explore its feasibility and superiority using as ocular surface graft.Methods After observing the morphology of FBAM under light microscope and scanning electron microscope,we did primary culture of corneal epithelial cells,taking monolayer fibrin-binding amniotic membrane as carrier,then observed cell growth overlay area.The elongation nature of FBAM was tested on electronic universal testing machine by calculating elastic modulus,using bilayer amniotic membrane(BAM)and monolayer amniotic membrane(MAM)as controls.Results The fresh FBAM was integrated as a whole,with structurally complete fibrin and amniotic membrane as well as tight binding of fibrin to amniotic membrane.The reserved FBAM was structurally complete,brittler than the fresh FBAM,while amniotic membrane still bound tightly to the fibrin,but under microscope the fiber reticular formation was fairly fuzzy.Single layer of epithelial cells was formed on FBAM after about seven-day culture in vitro.Disparity of cell growth overlay area was significant(P
4.MICROVASCULATURE OF THE DENTAL PULP OF RAT INCISORS
Bingyou ZHEN ; Jinxing KE ; Yun DU
Acta Anatomica Sinica 1955;0(03):-
By using replica cast SEM method, the vascular architecture of the dental pulp of rat incisors was studied. The characteristics of the vascular architecture are as follows:1. The axis of the dental pulp is occupied by the central vascular bundle, which consists of 10 to 20 arterioles and 8 to 12 venules.2. The arterioles (30-50?m in diameter) and the venules (50-80?m in diameter) located in the central bundles are arranged parallely in a relatively long distance as a countercurrent system. The longest distance is about 12 mm.3. Each arteriole of the central vascular bundle supplies an independent area of the dentin of the tooth. The territory of an arteriole is about 700 to 1400 ?m in length.4. The arteriole of the central bundle breaks up into capillaries (4~6?m in diameter) which are mainly distributed over the odontblast area and form a complex 3-dimensional plexus.
5.The detection and analysis of antinuclear antibodies in systemic sclerosis
Liqun HUANG ; Linjian KE ; Gaozhe ZHEN
Chinese Journal of Postgraduates of Medicine 2007;30(z2):12-13
Objective To evaluate ANA and ANAs as a indacation of systemic sclerosis diagnostic.Method ANAs were tested in 58 patients with SSc and 20 healthy controls by indirect immunofluorescence (ⅡF)and immunoblot(IB).Results ⅡF-ANA results indicated that the positive of ANAs were 98.3%(57/58)in SSc,and ANA pattern were mainly homogeneous-nucleolar,IB-ENA results indicated that the antinuclear antibodies were mainly anti-Scl-70.anti-SSA and anti-nRNP in SSc.The positive of them were 63.8%、31.O%and 5.2%.Conclusions It suggested that the patients might have other autoimmune diseases or the dangerous of other autoimmune diseases,and the IB kits of euroimmun got better positives and specificities to ANAs,the method could provide more helps to diagnosis of SSc.
6.MICROVASCULATURE OF ADRENAL MEDULLA IN RAT AND MOUSE
Bingyou ZHEN ; Shuzhen YANG ; Jinxing KE ;
Acta Anatomica Sinica 1954;0(02):-
The microvascutature of the adrenal medulla in the rats and mice were studied by scanning electron microscopy of vascular replicas.There are portal venules between the adrenal cortex and medulla,which are the side branches of the collecting venules of the adrenal cortex and break into capillaries at the adrenal medulla.The adrenal medulla in mice is mainly supplied by these portal venules,and in a rat adrenal medulla,there are also 5—8 medullar arterioles besides the portal venules.Ring-like constrictions appear to be the characters of sphincter at the ending parts of the portal venules,the branches of the medullar arterioles,the collecting venules,and the medullar venules which drain the medullar capillaries as well.The authors believe that the adrenal cortex controls the medulla by the way of the portal venules.
7.Effects of noxious coldness and non-noxious warmth on the magnitude of cerebral cortex activation during intraoral stimulation with water.
Xiuwen YANG ; Hongchen LIU ; Ke LI ; Zhen JIN ; Gang LIU
West China Journal of Stomatology 2014;32(6):552-555
OBJECTIVEWe used functional magnetic resonance imaging (fMRI) to explore the effects of noxious coldness and non-noxious warmth on the magnitude of cerebral cortex activation during intraoral stimulation with water.
METHODSSix male and female subjects were subjected to whole-brain fMRI during the phasic delivery of non-noxious hot (23 °C) and no- xious cold (4 °C) water intraoral stimulation. A block-design blood oxygenation level-dependent fMRI scan covering the entire brain was also carried out.
RESULTSThe activated cortical areas were as follows: left pre-/post-central gyrus, insula/operculum, anterior cingulate cortex (ACC), orbital frontal cortex (OFC), midbrain red nucleus, and thalamus. The activated cortical areas under cold condition were as follows: left occipital lobe, premotor cortex/Brodmann area (BA) 6, right motor language area BA44, lingual gyrus, parietal lobule (BA7, 40), and primary somatosensory cortex S I. Comparisons of the regional cerebral blood flow response magnitude were made among stereotactically concordant brain regions that showed significant responses under the two conditions of this study. Compared with non-noxious warmth, more regions were activated in noxious coldness, and the magnitude of activation in areas produced after non-noxious warm stimulation significantly increased. However, ACC only significantly increased the magnitude of activation under noxious coldness stimulation.
CONCLUSIONResults suggested that a similar network of regions was activated common to the perception of pain and no-pain produced by either non-noxious warmth or noxious coldness stimulation. Non-noxious warmth also activated more brain regions and significantly increased the response magnitude of cerebral-cortex activation compared with noxious coldness. Noxious coldness stimulation further significantly increased the magnitude of activation in ACC areas compared with noxious warmth.
Brain ; Cerebral Cortex ; Cerebrovascular Circulation ; Hot Temperature ; Humans ; Magnetic Resonance Imaging ; Mouth ; Pain ; Water
8.Microvasculature of Guines Pig's submandibular Gland
Bingyou ZHEN ; Qinxing KE ; Yuan DU ; Weiling MO
Journal of Third Military Medical University 1983;0(04):-
The microvasculature of the submandibular glands of guinea pig's was studied with the replica-SEM msthod. Special attention was paid to the microvascular architecture around the glandular ductal tree, which is mainly dichotomous, but usually asymmetrical and irregular, with a divergent angle of 40-80? and 7-8 grades of branching. The ductal tree is surrounded with a periductal vascular plexus. The periductal plexus of the excretory ducts can be divided into two layers: the inner layer is formed by capillaries and the outer layer by veins, so called venous coat. This venous coat is complexly constructed with button veins, loop veins and complicated venous circulatory by-passes. The capillary plexus of the striated ducts and intercalated ducts is supplied by venules with the cha-ractristics of the portal vein as they come from the capillaries of the acini. There are complex A-V and V-V anastomoses at the venous coat and at the lobu-lar porta.The significance of the microstructures described above was discussed.
9.The evaluation value analysis of serum CEA and CA15-3 levels in breast cancer on chemotherapy curative prognosis evaluation
International Journal of Laboratory Medicine 2017;38(18):2537-2539
Objective To investigate the value of serum CEA and CA15-3 in the evaluation of breast cancer chemotherapy and prognosis.Methods 63 breast cancer patients were retrospectively analyzed from May 2011 to June 2012 in our Hospitals as breast cancer group,according to the situation of survival would be divided into survival group(n=42) and death group(n=21) and select indicators for outpatient medical normal crowd 63 cases as normal group.Serum levels of CEA and CA15-3 were measured by fluorescence immunoassay for 4 years.The prognosis of all patients was analyzed statistically.Results Serum level of CEA and CA15-3 in Ⅳ period breast cancer patients was obviously higher than Ⅲ period,the difference was statistically significant(P< 0.05).Chemotherapy efficient of CEA,CA15-3,CEA and CA15-3 express in negative group were obviously higher than positive group,the difference was statistically significant(P<0.05).All patients were followed up for 1 to 4 years with an average follow-up time of 3.63 years and 42 patients survived for more than 4 years.CEA positive group patients of 4 years survival rate(55.26%) was obviously lower than negative group(84.00 %),the difference was statistically significant(P<0.05).CA15-3positive group patients of 5 years survival rate(52.78%) was obviously lower than negative group(85.19 %),the difference was statistically significant(P< 0.05).CEA and CA15-3 positive group patients of 5 years survival rate (52.38%) was obviously lower than negative group (95.24%),the difference was statistically significant (P < 0.05).Before treatment the serum CEA and CA15-3 level Survival groups was lower than death group,the difference was statistically significant(P<0.05).Conclusion Detection of serum CEA and CA15-3 levels in patients with breast cancer before chemotherapy can provide reference data for chemotherapy efficacy and prognosis evaluation.Detection of breast cancer patients with chemotherapy before serum CEA and CA15-3 levels can provide reference for chemotherapy curative effect and prognosis assessment data.
10.Cleft lip and cleft palate in Jangxi province: A case-control study on familial occurrence of 1 967 cases
Xing KE ; Yu LI ; Xindi JIANG ; Zhen TANG ; Linlin CHEN
Journal of Practical Stomatology 2016;32(5):712-717
Objective:To assess whether familial occurrence has an influence on the morphological characteristics of patients with nonsyndromic cleft lip and/or palate (NsCL/P).Methods:A case-control analysis was performed on the morphological characteristics of familial group and sporadic group,using medical records of 1967 patients with NsCL/P treated in the Affiliated Stomatological Hospital of Nanchang University from 2002 to 2014.Results:164 (8.34%) cases presented a positive history of cleft in their families.The cleft types,the positive familial rate of cleft lip only (CLO),cleft lip and alveolar ridge(CLA),cleft lip and palate (CLP) and cleft palate only (CPO) were 8.11%,8.54%,6.19% and 9.65% respectively.A positive family history of NsCL/P was associated with 0.66 times risk of CPO (P =0.036,OR =0.66,95% CI 0.44-0.98) compared to those of CLO,CLA and CLP.In familial group of CLP,the lateral incidence of male patients was different from that of female patients (P < 0.001).There was no significant difference between familial group and sporadic group on birth weights,parental child-bearing age and clinical manifestations of patients.Conclusion:Familial occurrence might have an influence on cleft type,laterality and gender of the patients with NSCL/P.