The subjective symptoms, various blood components, radioallergosorbent test (RAST) and others were examined for the patients who developed nasal allergy each year during the time of artificial apple pollination (before artificial pollination, during the peak of pollination and one month after pollination) compared with those for a control group, and the following results were obtained.
1) The symptoms, the time of their appearance and the kind of symptoms were the same as those of the apple pollinosis.
2) The change in the pattern of the eosinophile count was the same as that of the subjective symptoms.
3) However, only 2 of the 11 patients had a RAST score of suspicious positive, and no difference was detected between these patients and the control group.
From these findings, the many cases of suspected apple pollen allergy were cosidered to have been sensitized by other pollen which have common antigenicity to apple pollen in addition to true apple pollinosis, or to be mistaken diagnosis of other pollen allerg.

Eleven feamle distance runners were assessed for body composition, limb composition, and skinfold thickness. Body composition was measured by underwater weighing method. The cross-sectional area of subcutaneous fat and muscle in forearm, upper arm, leg, and thigh were obtained by using ultrasonic system. Skinfold thicnesses of seven sites were determined with ultrasonic apparatus. Runners averaged 157.0 cm in body height, 50.2 kg in body weight, 18.9% in relative body fat, and 40.6 kg in lean body mass. When compared untrained normal women, runners had significantly lower relative fat. However, there were no significant differences in lean body mass between runners and untrained. Within runners the more successful performers possessed lower relative body fat. Runners showed lower values of skinfold thickness than that of untrained for all measurement sites. The greatest differences in skinfold thickness between runners and untrained was found in side abdomen. Subcutaneous fat area of runners were significantly smaller than that of untrained. However, runners showed significantly larger muscle area for leg only compared with untrained. These results in body and limb composition of female distance runners may be mainly reffered to the characteristics of aerobic running training.

Utility of heart rate (HR)-oxygen consumption (VO₂) method to estimate energy expenditure (EE) was studied for 5 main apple working days based on 24 hour HR recording and EE by this method partially was compared with RMR (relative metabolic rate) method currently used in Japan. Results were as follows:
1. Heart rate during the apple cultivating work were 90-110 beats per minute, that were ranked with moderate work classified by Rodahl.
2. The values of EE estimated by HR-VO₂ method in every apple working days were almost suited for that of previous reports by RMR method.
3. Comparing EE by HR-VO₂ and RMR method for an example in harvesting work, 2, 654 kcal in former was 376 kcal (16.5%) higher than latter. Correlation coefficient was 0.987 (p<0.001) between both method with EE estimated in every working contents.
4. The intense of activity in a working day from view point of daily activity index (1-EE/basal metabolism) followed the order of harvesting putting sack on≅pluking flower>thinning apple> pruning.
Consequently, estimation of EE by HR-VO₂ method was precisely reflected metabolic state in human by comparison in spite of some defects. After this it was expected to use the HR-VO₂ method instead of RMR method.