1.Biochemical and immunohistochemical changes in gastrocnemius muscle of rats fed low protein diet following electrical stimulation.
TOSHIMI MIZUNUMA ; KENTARO SAKAI ; NORIKO KAWAHITO ; HIROTOSHI IWASHINA ; SHIGERU YAMAMOTO ; YUKARI KAWANO ; SACHINOBU MANABE ; KAZUE NAGATA-KUNO
Japanese Journal of Physical Fitness and Sports Medicine 1997;46(5):471-478
We investigated biochemical and morphological changes in electrically stimulated gastrocnemius muscle of rats fed normal (20% casein) and low-protein (5% casein) diets. Tissue weight and protein content per tissue weight of gastrocnemius muscle were significantly decreased in rats fed 5% casein diet than in rats fed 20% casein diet. Immunohistochemically, cathepsin B antigen was detected in inflammatory cells, and injured sites determined immunological intensity of anti-cathepsin B were lower in 5% casein diet group than 20% casein diet group. Electron microscopically, there was delayed repairment of muscle components (sarcometric units) in 5% casein diet as compared with that in 20% casein diet group.
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