“Exercise Guideline 2006” was published by Ministry of Health, Labour and Welfare of Japan to maintain and promote the health of people and to prevent lifestyle-related diseases through improving their habits of physical activity and exercise. In “Exercise Guideline 2006”, define ‘Ex’ as a unit of quantity of physical activity, and recommend to increase the ‘Ex’ value for physical activity to maintain the health of people. We investigated the effect of physical activity instruction using this new guideline on the profiles of dyslipidemia outpatients.We gave monthly physical activity instructions to 25 outpatients with dyslipidemia according to “Exercise Guideline 2006” and evaluated body composition and blood profiles 6 months after the instruction was started.Weekly physical activities were significantly increased by the instruction, from 7.1±4.1 Ex (pre) to 15.5±10.6 Ex (post). As the increased amount of physical activity in each subject was varied with individuals, we classified the subjects into two groups (increased group, or non-increased group) according to the increased amount of activity. In the subjects in increased group, significant decrease in body weight and serum triglyceride, and significant increase in serum HDL cholesterol were observed at 6-month point. There is no correlation between the variations in body compositions and the variations in triglyceride or HDL cholesterol levels.In conclusion, we suggest that physical activity instruction using “Exercise Guideline 2006” might be a useful and convenient strategy to improve lipid profiles in dyslipidemia patients.

Erythropoietin (EPO) promotes red blood cell production thereby raising oxygen transport ability. Epo gene expression is positively controlled by hypoxia-inducible factor (HIF) through the HIF binding site in an Epo gene enhancer; and negatively controlled by GATA, which binds to the GATA site in the Epo gene promoter. Drugs that activate HIF (FG-2216) or inhibit GATA (K-11706) increase the production of EPO. Therefore, these drugs might be illicitly used to improve performance in sports as new doping practices. To develop a system for detecting such a doping strategy, we performed DNA microarray and quantitative RT-PCR to compare the effects of FG-2216^*, the derivative of FG-2216, and K-11706, on gene expression with that of recombinant human EPO (rhEPO) or hypoxia in mice. Gene expression analysis in bone marrow cells showed increased expression of Lactoperoxidase (Lpo) only in the mice treated with K-11706, and decreased expression of Oncostatin M (Osm) with FG-2216^* or hypoxia, but not with K-11706. Results to date suggest that gene expression changes observed in using these drugs have the potential to yield novel biomarkers in a detection system for new illicit drug use.

【Objective】The purpose of this study was to investigate the acute responses of adiponectin and leptin to resistance exercise in healthy elderly men. 【Methods】Eight healthy men (age, 25.5±3.3 yrs) participated in this study. They performed two trials. Trial 1 was an exercise session consisting of 5 resistance exercises at high-intensity (75% one-repetition maximum : 1 RM) with three sets of 10 repetitions. Trial 2 was a control trial at rest for the same time as trial 1. Blood samples were taken to assess adiponectin, leptin, and metabolism related substances (glucose and lipids).【Results】Leptin significantly decreased 24 h after the resistance exercise but did not change in trial 2. Blood insulin and lactate concentrations significantly increased immediately (0 min) and 30 min after the exercise. However, adiponectin, glucose and lipids were not affected. 【Conclusion】Adiponectin was not affected by resistance exercise, whereas leptin decreased 24 h after the exercise. This suggests that leptin may have quite a delayed response to high-intensity resistance exercise.