0.05) in the BMT group but significantly enlarged in the CON group (50.4?6.0 mm vs. 55.2?7.1 mm, P

Objective:To explore the correlation with neonatal congenital cytomegalovirus infection,maternal primary infection and secondary infection.Methods: 48 neonates with congenital CMV infection were assigned to infection group with their mothers.And the other 30 couples without congenital CMV infection were assigned to negative group with their mothers.The level of CMV-IgM/IgG and affinity of CMV-IgG in peripheral blood were tested by CLIA,and CMV-DNA in mother′s milk,peripheral blood and urine of the newborn was tested by fluorescent quantitation PCR.We also analyzed the differences of the test results between the two groups and performed a retrospective analysis to compare the levels of CMV-IgG of the mother with early pregnancy with the result of this test.Results: In the infection group,the level of CMV-IgG in peripheral blood and CMV-DNA in milk was significantly higher than those in the negative group,the difference was statistically significant(P<0.01).Ratio of CMV-IgG antibody in newborn babies and their mothers in the infection group was lower than the other group,the difference was statistically significant(P<0.01).There was negative correlation of IgG level between the newborn babies and their mothers in the infection group,the difference was statistically significant(P<0.01);While in the positive correlation in the negative group,the difference was statistically significant(P<0.01).The CMV-IgG concentrations of the mothers with early pregnancy was significantly lower than that of this infection group,the difference was statistically significant(P<0.01);but in the negative group,there was no significant difference between the CMV-IgG level of the mothers with early pregnancy and the results of this test(P>0.05).Conclusion: It is a high-risk factor for neonatal congenital cytomegalovirus infection that CMV-IgG level of the pregnant women is promted by the reactivation or reinfection of cytomegalovirus.It is important to monitor CMV-IgM/IgG during pregnancy.

Objective To evaluate the safety and efficacy of bone marrow cells(BMCs) transplantation on the improvement of cardiac function and myocardial perfusion in patients with old myocardial infarction. Methods and Results A total of 10 patients with anterior wall myocardial infarction were transplanted with autologous mononuclear BMCs through an infusion catheter which was placed with the tip in the left anterior descending coronary artery after coronary angiography and interventional therapy if necessary. After 3 months of follow-up, a significant increase of left ventricular ejection fraction(LVEF) determined by ultrasonic cardiography was found (54.5%?6.5% vs. 45.3%?9.8% before transplantation, P=0.003); 201-thallium scintigraphy (SPECT) showed that ventricular myocardial perfusion was significantly improved as the semiquantitative score of the immediate and delayed myocardial perfusion defects decreased from 29.5?5.8 and 28.6?6.3 to 23.9?5.7 and 23.0?6.1prospectively (both P

Objective:To investigate the causes of false-positive anti-HCV in non-syndromic cleft lip and palate with serum chemiluminescence test. Methods:We performed a hospital-based,case-control study of 4 050 patients with non-syndromic cleft lip and palate and 8 547 control patients without cleft lip and palate to evaluate associations between positive anti-HCV and non-syndromic cleft lip and palate. CLIA test were used for detect anti-HCV in control group. RIBA and FQ-PCR were used for confirming the result of the positive samples of CLIA test. CLIA test were used for detect anti-HCV in case group until CLIA test was negative. IgG,RF and ANA were used for case-control comparisons. Results:The detection rate of anti-HCV were higher in the case group than that of control group (P<0. 05). The false-positive rate was 1 percent based on testing of 4 050 patients. These samples were negative after opration 1-19 months. There were no change among IgG, RF and ANA in the false-positivepatients. Conclusion: False-positive anti-HCV in non-syndromic cleft lip was possible associated with abnormal plasma metabolic product. It can be excluded by track monitoring.

Comparison of assays of immunoreactive insulin (IRI) and specific insulin in evaluating islet β cell function and insulin sensitivity suggested that there were no significant differences in individuals with different glucose tolerance impairment by two assays. The evaluation of islet β cell function using IRI and insulin sensitivity is still valid in clinical practice.

Objective To describe the clinical characteristics,outcome and prognostic factors of primary intraspinal tumor treated with postoperative radiotherapy.Methods Thirty-three patients diagnosed as suffering from intraspinal tumor treated with postoperative radiotherapy were analyzed.There were eighteen male and fifteen female,with a median age of 37 years(range 7-64 years).Two patients were treated with grossly total resection,25 subtotal resection,2 biopsy only.These patients were treated either with(~(60)Co)(n =18,before 1998) or 6?MV-X ray(n =15,after 1998) to the total dose of 45-68?Gy.The overall response and survival rates were calculated by Kaplan-Meier method and the difference between groups was tested by the log-rank method.The prognostic factors related to the characteristics of patient,tumor,and treatment were determined.Results The overall response,five-year and disease-free survival rate was 81.3%,59.4% and 56.3%,respectively.By multivariate analysis,the histological type(P= 0.039) and gender(P= 0.049) had independent impact on the 5-year survival rate.Ependymoma had a better prognosis compared to other histological subsets and female gained more survival advantage over male.There was no close relationship between survival and other factors,such as age,extent of surgery,initial site,irradiation dose,or the interval between surgery and radiotherapy.Conclusions Postoperative radiotherapy is safe and effective for primary intraspinal tumor.The histological type and gender are important prognostic factors of survival in primary intraspinal tumors.

Objective:To investigate the diagnostic and terapeutical value of FPG,GA,HbA1c and GA/HbA1c ratio in T1DM/T2DM.Methods:The study was made by case-control method.In our study,30 healthy subjects were selected from health physical ex-amination as control group while 160 diabetics were selected as case group,in which there are 76 TIDM and 84 T2DM.Analyzing the difference of relevance of FPG,GA and HbA1c,the difference of GA/HbA1c and threshold of the case and the control,and this analysis was also used between the T1DM and the T2DM.The data was managed by independent-sample t test,ROCK and Pearson correlation test of SPSS.Results:The results of FPG,GA ,HbA1c and GA/HbA1c ratio of T1DM and T2DM were significantly higher than those in the control group(P<0.01).And they were higher in the T1DM than in the T2DM(P<0.01);in the T1DM group,GA was strongly positive correlative with HbA1c(P<0.01),FPG was weakly positive correlative with GA(P>0.05),and weakly negative correlative with HbA1c(P>0.05);in T2DM group,there were positive correlation among FPG,GA and HbA1c(P<0.05),the degree of correlation ranked as HbA1c/GA>FPG/GA>FPG/HbA1c;analyzing the ROC of measures in T1DM group,the sensitivity and specificity were re-spectively 86.8% and 100% for diagnosing DM when FPG threshold was set on 5.86 mmol/L ( AUC=0.922 ) ( P<0.05 ).The sensitivity and specificity were both 100% for detecting DM when GA threshold was set on 15.5%( AUC=1 ) ( P<0.05 ).The sensitivity and specificity were respectively 98.7%and 100%for diagnosing DM when HbA1c threshold was set to 6.10%( AUC=1) ( P<0.05).The sensitivity and specificity were respectively 93.4% and 100% for diagnosing DM when the threshold of GA/HbA1c was set on 2.95 ( AUC=0.992 ) ( P<0.05 );analyzing the ROC of measures in T2DM group, the sensitivity and specificity were respectively 91.7% and 100% for diagnosing DM when FPG threshold was set on 5.94 mmol/L ( AUC=0.941 ) ( P<0.05 ).The sensitivity and specificity were respectively 85.7%and 100%for diagnosing DM when GA threshold was set on 15.5%( AUC=0.977) ( P<0.05).The sensitivity and specificity were respectively 97.6% and 100% for diagnosing DM when HbA1c threshold was set on 5.95%(AUC=0.991)(P<0.05).The ratio of GA/HbA1c had no diagnostic cutoff point,AUC was 0.644(P>0.05).Conclusion:FPG,GA, HbA1c and GA/HbA1c ratio are of high value in monitoring of blood glucose, diagnosis and typing in T1DM and T2DM.There are missed diagnosis when we diagnose T1DM and T2DM by the upper limit of reagent instruestion of FPG,GA,HbA1c.It is more important for a person with T1DM to monitor FPG than others.

Objective:To observe the effect of tuina manipulations on blood pressure and its variability in hypertension patients. Methods:Forty hypertension patients were randomized into an observation group and a medication group, 20 cases in each group. The observation group was intervened by tuina manipulations of kidney-tonifying blood-circulating and collaterals- unblocking in addition to regular medication, while the medication group was by the same medication. The 24-hour blood pressure monitoring was performed before intervention and after 3-month intervention. The blood pressure and its variability were observed and compared. Results:There were no significant differences in comparing the blood pressure and blood pressure variability between the two groups before intervention (P>0.05); after 3-month intervention, the blood pressure and its variability were significantly improved in both groups (P<0.05); the improvements in the observation group were more significant than those in the control group (P<0.05). Conclusion:Tuina manipulations of kidney-tonifying blood-circulating and collaterals-unblocking plus medication can produce a better effect than regular medication in promoting blood pressure and its variability, and this method is worth applying in clinic as it’s easy-to-operate and has no adverse effect.

Objective To analyze the distribution and drug resistance characteristics of pathogenic bacteria isolated from blood cultures in the infected patients in our hospital during 2009-2013 to provide the newest evidence for the clinical anti-infection ther-apy.Methods The bacterial identification and drug susceptibility test were performed by applying the VITEK-32 System.The dis-tribution situation and the drug susceptibility test results of pathogens isolated from blood culture specimens in our hospital during this period were analyzed.Results 2 301 strains of positive bacteria were isolated from 14 006 cases of blood culture during these five consecutive years.The positive rate was 16.4%.Among them,1 303 strains were Gram positive bacteria(56.6%),954 strains of Gram negative bacilli(41.5%)and 44 strains of fungi(1.9%).Gram-positive bacteria were mainly Staphylococcus aureus,coagu-lase negative staphylococci(CNS),etc.Gram-negative bacteria were mainly E.coli,Klebsiella pneumoniae,etc.E.coli and Klebsiella pneumoniae were highly sensitive to carbapenem,amikacin,cefoxitin and antibacterial drugs containing enzyme inhibitor.The detec-tion rates of extended-spectrumβ-lactamase(ESBL)-producing Escherichia coli and Klebsiella pneumoniae were 55.8% and 18.4%respectively.Acinetobacter baumannii in blood culture had serious resistance to most of antibacterial drugs.Conclusion The kinds of pathogens isolated from blood culture are complex with different sensitivity to antibacterial drugs.Timely understanding the re-sults of blood culture has the important significance to adjust the treatment scheme in clinic and timely discover drug-resistant strains for serve clinic better.

The curriculum of oncology radiotherapy covers basic radiology,clinical oncology and other aspects.With the development of new radiation therapy technology and the extensive application of computer technology in the field of radiotherapy,the traditional lecture-based teaching model has not adapted to the rapid development of the needs of oncology radiotherapy any more.Teachers in the first affiliated hospital of Soochow university integrated computer multimedia with problem-based learning in the teaching of oncology radiotherapy.With those innovations,the quality of teaching as well as the creative and self-learning abilities of students have been enhanced.