Objective To understand the level of clinical first aid ability of nurses in non-emergency department of third-class general hospitals of Taiyuan City, and to provide theoretical basis for effective intervention of nursing managers. Methods A questionnaire survey was conducted among 1008 nurses in 5 third-class general hospitals in Taiyuan city. Results The total score of clinical first aid ability of the non-emergency department nurses in 5 third-class general hospitals of Taiyuan was (121.29 ± 12.19) points.There were significant differences in scores of different age, education, nursing age, grade, title and departmental nurses (F=25.584-128.611, P<0.01). There was only a difference in the theoretical knowledge reserve in different department (F=3.589, P<0.05). The effect of the first aid ability on the emergency first aid ability of the non-emergency department nurses was the energy level, the education degree and the first aid ability of the nurses are increasing with the education level and the energy level. Conclusions In clinical work, nursing managers should pay attention to the development of first aid ability of low-grade and low-educated nurses in non-emergency department, and can carry out the training for first aid and comprehensive ability by situation simulation, emergency plan exercise, process management and so on.

Rv2742 is a novel gene identified from Mycobacterium tuberculosis H37Rv by the proteogenomics strategy. The aim of this study was to establish a system of soluble expression and purification of the missing protein Rv2742 in M. tuberculosis H37Rv, to provide reference for further research on the biological function of Rv2742. The soluble protein was not successfully induced by prokaryotic expression vectors pGEX-4T-2-Rv2742, pET-32a-Rv2742, pET-28a-Rv2742 and pMAL-c2X-Rv2742. After the codon of novel gene Rv2742 was optimized according to E. coli codon usage frequency, only the recombinant strain containing plasmid pMAL-c2X-Rv2742 could produce soluble products of Rv2742 encoding gene. In addition, the expression effects of the desired fusion protein were also analyzed under different conditions including hosts, culture temperatures and IPTG concentrations. The optimum expression conditions were as follows: Rosetta (DE3) host, 16 °C culture temperature and 0.5 mmol/L IPTG. After being purified by affinity chromatography with amylose resin, the fusion protein sequence was confirmed by LC-MS/MS. These results indicated that the novel gene Rv2742 product could be successfully induced and expressed in a soluble form by the expression system pMAL-c2X with MBP tag. Our findings provide reference for studies on potential interaction and immunogenicity.
Chromatography, Liquid ; Cloning, Molecular ; Escherichia coli ; Mycobacterium tuberculosis ; genetics ; Recombinant Fusion Proteins ; Tandem Mass Spectrometry