Objective To investigate the infection status of mycoplasma in Shenzhen migrant workers′genitourinary tract and the sensitivity of 12 kinds of antibiotics for guiding rational use of drugs in clinical treatment ,through the test of specimens with 2 905 cases of mycoplasma culture and drug susceptibility test .Methods Drug sensitivity test and culture of mycoplasma were per-formed on the specimens form 2 905 new patients in five hospitals by using liquid mycoplasma culture method .Results In speci-mens of 2 905 cases ,mycoplasma positive rate was 41 .8 % (1 214 cases) ,single ureaplasma urolyticum(Uu) infection rate was 39%(1 133 cases) ,single mycoplasma hominis(Mh) infection rate was 1 .3% (37 cases) ,Uu+ Mh mixed infection rate was 1 .5% (44 cases) .The result of sensitive test show that the sensitive rate of Uu to josamycin ,doxycycline ,minocin were 88 .6% ,91 .9% , 92 .2% .Conclusion The migrant workers'mycoplasma infection rate is very high in Shenzhen area .The infection of single Uu has the highest percentage ,mycoplasma is sensitive to the minocin .

Objective To investigate the clinical significance of serum sICAM-1 level in patients with NSCLC.Methods Sera from 51 cases with different TNM stages of NSCLC were collected and analyzed for concentration of sICAM-1 with Sandwich ELISA method.Results Serum levels of sICAM-1 were significantly higher in NSCLC group than in healthy controls and correlated with the clinical stage of NSCLC cases.Conclusions sICAM-1 could be an important factor which underlies tumor invasion and metastasis and may have a prognostic value in lung cancer.

Objective To understand the level of clinical first aid ability of nurses in non-emergency department of third-class general hospitals of Taiyuan City, and to provide theoretical basis for effective intervention of nursing managers. Methods A questionnaire survey was conducted among 1008 nurses in 5 third-class general hospitals in Taiyuan city. Results The total score of clinical first aid ability of the non-emergency department nurses in 5 third-class general hospitals of Taiyuan was (121.29 ± 12.19) points.There were significant differences in scores of different age, education, nursing age, grade, title and departmental nurses (F=25.584-128.611, P<0.01). There was only a difference in the theoretical knowledge reserve in different department (F=3.589, P<0.05). The effect of the first aid ability on the emergency first aid ability of the non-emergency department nurses was the energy level, the education degree and the first aid ability of the nurses are increasing with the education level and the energy level. Conclusions In clinical work, nursing managers should pay attention to the development of first aid ability of low-grade and low-educated nurses in non-emergency department, and can carry out the training for first aid and comprehensive ability by situation simulation, emergency plan exercise, process management and so on.

Objective To understand Shenzhen Longgang,guangming and longhua new district four district hospital ICU pa-tients with secondary pulmonary tuberculosis merger lower respiratory infection of pathogenic bacteria distribution and drug resistance status of provide a reference for clinical diagnosis and rational use of antibiotics therapy.Methods Random selec-tion from February 2013 to October 2015 in the three district hospital ICU diagnosis of secondary pulmonary tuberculosis patients with lower respiratory infection in 593 cases of sputum specimen pathogenic bacteria culture and drug susceptibility results were retrospectively analyzed.Results 593 cases of ICU secondary pulmonary tuberculosis patients with respiratory tract infection of the communist party of China isolated 617 strains of pathogenic bacteria,fungi accounted for 49.6% (306/617),gram negative bacilli accounted for 40.4% (249/617),gram positive cocci accounted for 10.0% (62/617).Fungal in-fection main pathogens for white smooth candida yeast and candida yeast,respectively accounted for 44.2% (273/617)and
4.5% (28/617),gram negative bacillus mainlyKlebsiellaPneumoniae,Pseudomonasaeruginosa,and H.influenzae,respec-tively accounted for 16.7% (103/617),12.0% (74/617)and 7.3% (45/617),gram-positive cocci mainly for Saphylococcus aureus and Epidermisstaphylococcus and Hemolyticstaphylococci,respectively accounted for 4.5% (28/617),3.2% (20/617)and 0.9% (5/617).Pathogenicbacteria isolated from the multiple drug resistant bacteria,present different levels of resistance to commonly used antimicrobial agents.Conclusion ICU patients with secondary pulmonary tuberculosis merger of lower respiratory tract infection pathogens to fungi and gram-negative bacilli,the most commonWhite candida,Klebsiella pneumoniae,and Pseudomonasaeruginosa,and different levels of resistance to commonly used antimicrobial agents.

Objective To investigate the reliability and validity of the assessment questionnaire on clinical first-aid ability of nurses in non-emergency department and provide a scientific tool for the evaluation of clinical first-aid ability of nurses in non-emergency department. Methods General information questionnaire and the non-emergency department nurses' clinical first-aid ability assessment questionnaire were applied on 1008 nurses. Split-half reliability,internal consistency reliability and retest reliability were used to test the reliability of the questionnaire. The content validity and structure validity were applied to test the validity. Results The Cronbach's α coefficient,split-half reliability coefficient and retest reliability coefficient of the questionnaire were 0.927,0.861 and 0.977. The correlation coefficients among the various dimensions were from 0.298 to 0.810. The correlation coefficients between dimensions and total score were from 0.534 to 0.947. Exploratory factor analysis was performed to select five factors. The cumulative variance contribution rate was 58.28%. Conclusions The questionnaire has good reliability and validity with reasonable structure. It can be used as a tool to assess clinical first-aid ability of nurses in non-emergency department.

Aim To investigate the effect of theaflavin on oxidized low density lipoprotein(ox-LDL)-induced foam cell formation and oxidative stress in THP-1 macrophages and its mechanism.Methods THP-1 derived macro-phages were pretreated with 50 μmol/L theaflavin and(or)10 μmol/L nuclear factor erythroid 2-related factor 2(NRF2)inhibitor ML385,then 100 mg/L ox-LDL was added to the cells for 24 h to establish the foam cell model.The effect of theaflavin on THP-1 macrophages viability was evaluated by CCK-8 assay and LDH release.The expression of inflamma-tory cytokines interleukin-6(IL-6),interleukin-1 β(IL-1β),tumor necrosis factor-α(TNF-α)were analyzed by real-time quantitative polymerase chain reaction(RT-qPCR)and Western blot.The release of inflammatory cytokines were detected by enzyme linked immunosorbent assay(ELISA).Intracellular lipid accumulation was detected by Oil red O staining,and lipid absorption was observed by DiL-labeled oxidized low density lipoprotein(DiL-ox-LDL)staining.Re-active oxygen species(ROS)level was detected by DCFH-DA probe.The expression of lipid uptake,cholesterol efflux and oxidative stress-related proteins were detected by Western blot and RT-qPCR.Results Treatment with 100 mg/L ox-LDL significantly decreased cell viability and cholesterol efflux-related protein expressions,increased lipid uptake,ac-cumulation and lipid uptake-related protein expressions,and significantly promoted inflammation and ROS level,as well as the expressions of myeloperoxidase(MPO),NADPH oxidase 2(NOX2)in THP-1 macrophages(all P＜0.05).After pretreatment with theaflavin,cell viability was increased,intracellular lipid uptake,accumulation and lipid uptake-related protein expressions were significantly reduced,cholesterol efflux-related protein expressions were significantly increased,the expression and release of IL-6,IL-1β and TNF-α were significantly decreased,ROS level was significantly decreased,and the expression of MPO and NOX2 were decreased(all P＜0.05).Pretreatment with theaflavin effectively alleviated intracellular oxidative stress by altering the expression of NRF2,heme oxygenase-1(HO-1)and Kelch-like ECH-associated protein 1(KEAP1)in NRF2 signaling pathway,and enhanced the translocation of NRF2 into the nucleus.After pretreat-ment with ML385,the expression levels of NRF2,HO-1,KEAP1 and CD36 were significantly decreased.Conclusion Theaflavin can significantly inhibit ox-LDL-induced foam cell formation,inflammation,and oxidative stress through NRF2/HO-1 signaling pathway in THP-1 macrophages.