The effects of RHPS & APS on immune function in normal mice and immunosuppressed mice were studied. The results showed that RHPS and APS enhanced not only the phagocytosis of peritoneal mac-rophages in normal mice, but also antagonized completely the immun- Osuppresslve effect of prednisoione. RHPS and APS increased both the lymphocyte transformation rate induced by PHA and ANAE+ rate in normal mice, and antagonized also the suppressive effects of GY or PDS. No significant influence was found on the proliferation of rabbit lymphocyter treated with PHA in vitro. It seems that no obvious difference of effect was found on immune function between RHPS and APS.

Objective: To observe the improvement of symptom and cardiac function after intervention on depressive mood in patients with myocardial infarction (MI) Methods: Among 76 patients with MI, 47 with depressive mood (according to results of SDS and HRSD) were randomly divided into intervention group (n=26) and control group (n=21), the former received health education and supportive therapy, some with major depression received antidepressant for 3 months Three months later, both groups were assessed with SDS and HRSD again, as well as assessments of symptoms of heart disease and cardiac function Results: The scores of SDS and HD in both groups were lower 3 month later (P

Objective To observe and compare the influences of radiofrequency catheter ablation (RFCA) and through-the-balloon ultrasound ablation (TTB-USA) on coagulable states of blood and evaluate their safety. Methods Eleven mongrel canines of either sex were divided into TTB-USA group (n=5) and RFCA group (n=6). We measured the expression of alphagranule membrane protein (GMP-140) on the surface of activated platelets by flow cytomety before and after cannulation, immediately after ablation, 30 minutes and 48 hours later, respectively. And at the same time,we also examined the change of tissue type plasminogen activatior (T-PA)? plasmingen activator inhibitor-1 (PAI-1). Results The expression of GMP-140 elevated before ablation, reached its peak value at hour 48, but the degree of uprising was lower in TTB-USA group than in RFCA group. Both t-PA and PAI-1 uprising just after ablation, restored to normal 48 hours later, t-PA reached its peak just after ablation, but PAI-1 was 30 minutes later and there was no difference in uprising degree between the two groups. Conclusion Both TTB-USA and RFCA could activate platelets and result in the change of blood coagulable states, but TTB-USA was slighter than RFCA, so TTB-USA seems to be more safely in ablating pulmonary vein orifice than RFCA in treatment of focal atrial fibrillation.

With two recipes,i.e.nodule disease treated with revised Maimengdong Decoction and Xiaoluo Pill,sub-acute thyroid inflammation of granuloma treated with Haizao Yuhu Decoction and Xiaoluo Pill,it makes clinical research to discuss the causa morbi,mechanism,differentiation thought and the experiences of removing toxin,stasis,swelling,sputum,and softening mass in treating such refractory diseases.

Objective To evaluate the clinical efficacy of circumferential ablation at orifice of pulmonary veins with ultrasound energy in the cure of paroxysmal atrial fibrillation(PAF).Methods The study group included 5 patients with PAF who had significant symptom and were drug resistance,and ultrasound ablation was applied at the orifice of their pulmonary veins to get electrical isolation.Results Nineteen pulmonary veins got electrical isolation and among them 3 were combined with radiofrequency ablation.PAF recurred in one patients and atrial premature beats occurred in other two patients but all disappeared after being treated with amiodarone.During the ablation atrial perforation occurred in one patients and severity vague response in another patient.All patients reached a minimum of six months of follow up,and remained in sinus rhythm.Conclusions The circumferential ablation at orifice of pulmonary veins with ultrasound energy is effective in restoration and maintenance of sinus rhythm in patients with PAF.

It was found that the proliferation of mice L7712 leukemic cells in vitro was markedly inhibited by 5 mg/L GP1 and VP10 The inhib itory rate increased with the incubation time. At a concentration of 0 .04-20mg/L, the mitotic index ( MI ) of GP1 group increased, but the MI of VP16 group decreased. After L7712 cells were treated with GP, 5 mg/L for 12 h the MI reached the highest point which was 8 times as high as that of the control, at the same time, the MI of VP16 ( 5 mg/L) group was about one-third of that of the control. The result of the combination of GP1 with VP16 showed that VP16 could antagonize the effect of GP on MI of L7712 cells. After being treated by GP1 and VP18 for 24 h, serious damage of L7712 cells could be observed. Both drugs inhibited the incorporation of (3H) TdR into DNA of S180, ascitic hepatoma (AH), L1210 and L7712 cells incubated for 24 h. It was further observed that S180 and L7712 cells were more sensitive than other cells to both drugs.

The plysical and chemical characteristics of recombinant human GM - CSF (rhGM - CSF) were studied separatly. rhGM - CSF was treated by GdHCl, reduced by DTT, and the disulfide bond was blocked by idoacetamide. The results showed that the samples aren' t homogeneous in UV absorption spectrum and RP-HPLC analysis after treatment by DTT. There was no remarkable differences in the results of the analysis of SDS-PAGE electrophoresis and western blot between treated and untreated rhGM - CSF samples. The effect of GdHCl on GM-CSF was reversible in all above tests; In peptide mapping analysis, the digestion of the samples with blocked disulfide bond by CNBr and protease is more complete than that without any treatment.

Through the improvement on determination of rhTNF-? bioassay, we established the formal procedure . Methods; We choose L929 cell for rhTNF-? bioassay determination. To identify the influence of rhTNF-? on the growth of HL-60 cells, various concentration and effecting time were studied. Results: The results showed that the inhibition of rhTNF-? on HL-60 cells based on dose and time. Northern Dot Blot and DNA electrophoresis showed c-myc gene was surpressed, and DNA was damaged by rhTNF-?. Conclusion: Autometic calculation is responsible for bioassay of rhTNF-?. rhTNF-? could inhibit the proliferation of HL-60 cells and its mechanism is different from doxirubicin.

Objective: To establish National Standard of rhTNF-? complying with the requirements of research and quality control. Methods: National Standard of rhTNF-? were assayed against international standard (NIBSC) of rhTNF-? by cytotoxicity bioassay (L929 ) in vitro. The collaborative study has been carried out among four laboratories. Results: Based on the statistical analysis the results show that mean pf 95 % confidence interval is 3 289 ~ 4 266 IU per ampoule; the 95 % reference range is 1 735 ~ 8 087 IU per ampoule. Based on this study, the potency of rhTNF-? standard is defined as 3500 IU per ampoule. Stability tests indicate that the bioactivity has not been changed significandy under the storage of four different temperautres for 27 monthes. Conclusion: The preparation of rhTNF-? met the requirement of quality and could be used as a National Standard.

Objective To observe the influence of catheter ablation of the orifice of pulmonary veins (PVs) using ultrasound energy on hemodynamic parameters and explore the safety and efficacy of ultrasound ablation. Methods Nineteen PVs of six canines received ultrasound ablation, with a preset ablation temperature between 55 ℃-65 ℃, for 60 seconds. Ultrasound ablation catheter was positioned on the orifice of PVs via transseptal approach under fluoroscopic. Selective pulmonary venography was undertaken and the hemodynamic data were measured before ablation? immediately and three months later after the ablation.Results All except one canine completed the experiment. All the PVs got effective electrical isolation, and had no stenosis with pulmonary venography, no significant change in hemodynamic parameters was observed before, immediately or three months later after the ablation. Conclusion Catheter ablation within PVs by ultrasound energy was safe and efficient, it was a useful method to treat focal atrial fibrillation derived from PVs in clinical practice.