1.Inhibitory effect of SM-1 on human liver microsomal cytochrome P450 enzyme
Yanfen CHEN ; Jingxiu CHEN ; Meng SUN ; Junyao DING ; Zeneng CHENG ; Gaoyun HU ; Jinsong DING ; Qubo ZHU
Chinese Pharmacological Bulletin 2017;33(5):627-629
Aim To investigate the effect of SM-1 on seven main cytochrome P450(CYP450)in human liver microsomes.Methods Substrate or SM-1 was incubated with human liver microsomes for 30 min in vitro,and divided into control group and experimental group.The effects of SM-1 on the main phase I metabolic enzymes in human liver microsomes was detected by HPLC.Phenacetin,bupropion,paclitaxel,tolbutamide,omeprazole,dextromethorphan,testosterone were investigated as probe drugs.Results Inhibition rate of SM-1 on the classical substrate of human liver microsomal CYP was 0.05％,3.37％,0.08％,2.07％,4.20％,-0.15％and 10.84％,respectively.Conclusions SM-1 may have inhibitory effect on CYP3A4.Attention should be paid to the interaction of clinical drug induced by CYP enzyme inhibition.
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