Objective To study the effect of berberine on arrhythmia caused by stretch of rats' myocardium,in vitro,after myocardial ischemia (MI) for 30 min.Methods The study was carried out in the laboratory of Heilongjiang traditional Chinese medicine university.A total of 40 Wistar rats were randomly (random number) divided into 4 groups,namely normal control group,berberine group、MI group and MI + berberine group.After perfusion of rat' s heart with Langendorff perfusion device,the model of MI was made with ligation of left anterior descending branch for 30 min.Berberine was dissolved in Tyrode' s solution to a concentration of 300 μmol/L.The hearts were stretched for 5 s by 0.2 ml.The effect of stretching was observed for 30 s to record 90％ monophasic action potential (MAPD90),premature ventricular beats (PVB) and ventricular tachycardia (VT).Quantitative data were compared with ANOVA.Qualitative data were compared with a x2 test.Differences with a value of P ＜ 0.05 were considered statistically significant.Results MAPD90 in normal control and MI group obviously prolonged after the hearts were stretched ( P ＜ 0.01 ).And MAPD90 in MI group was even longer than that of normal control group (P＜0.05).Berberine had no influence on MAPD90 before myocardiuml stretched (P ＞0.05),while it could reduce the degree of prolongation in MAPD90 after myocardium stretched (P ＜ 0.05 or P ＜0.01 ).The incidence rate of PVB and VT in normal control and MI group increased after stretched.The berberine in dose of 300 μmol/L could reduce the incidence of PVB and obviously inhibit the occurrence of VT ( P ＜ 0.01 ).Conclusions Berberine could obviously inhibit the occurrence of stretch-inducedarrhythmias after myocardial infarction.

[Objective]To investigate feasibility and efficacy of clinical application of endoscopic anterior cervical decompression and fusion.[Method]With Metrx MED system and modified working tube and decompression instruments,endoscopic anterior cervical decompression and fusion were performed on 15 patients with cervical spondylotic myelopathy.A paramidline right transverse incision of 2 cm long was made.A channel between the neurovascular and visceral sheath was created through finger dissection.The dilators were inserted sequentially.An original one arm working tube or a modified,shortened working tube with double arms was inserted over the dilators.Then decompression and fusion with autologus graft was performed under endoscope.[Result]Once the tubular retractor was stably fixed throughout the operation,releasing of the discomfort was observed.All the compression tissue such as soft disc tissue or hard osteophyte and anterior longitudinal ligament or dura sac were clearly visualized under the endoscope.Shortened working tube provided improved visual field and increased manipulation space for the decompression instrument.The double arms enhanced stability of the working tube but could not eliminate moving of it during operation.Intraoperative blood loss was 200 ml to 800 ml.The operation lasted 80 to 210 minutes.The incision healed in one week.Postoperative discomfort in laryngeal region was light.All of the cases showed thorough decompression on postoperative MRI films and gained better spinal cord function at postoperative follow-up.No surgery-related complication were encountered.[Conclusion]Endoscopic anterior cervical decompression and fusion based on the use of a working tube is technically feasible.Compared with conventional method,this technique has the advantages of improved intraoperative visualization,thorough decompression,minimal trauma,quicker recovery and improved cosmetic result.Disadvantages waiting for further improving include:limited manipulation space,discomfort and potential hazards to the surrounding structure resulted from moving of the working tube.

AIM:To explore the effect of long-term (6 months) endogenous testosterone deprivation by orchidectomy on the function of voltage-dependent potassium channels of vascular smooth muscle cells in rats. METHODS:Wistar rats were raised for 6 months after castration. Isometric tension measurement of aortic rings,whole-cell patch-clamp technique and Western blotting analysis were employed to examine the functional and posttranscriptional alterations of voltage-dependent potassium channels. RESULTS:Voltage-dependent potassium channel blocker,4-aminopyridine,significantly decreased the constriction of aortic artery rings from male rats after 6-month castration. In castrated rats the amplitude of voltage-dependent potassium currents of aortic artery smooth muscle cells was significantly decreased compared with that in control rats. Meanwhile,the expression of Kv 1.5 channel protein,which plays an essential role in mediating vasomotor function,was also reduced. The functional and molecular alterations of voltage-dependent potassium channels were both restored when the rats were concomitant applied with physiological level of testosterone after castration. CONCLUSION:Long-term deprivation of endogenous testosterone in rats significantly attenuates the function of voltage-dependent potassium channels,and the decreases in expression of Kv1.5 channel protein accounts for this alteration. Long-term application of physiological concentration of testosterone,which recovered the impaired function of voltage-dependent channels,may be beneficial for male gender with hypotestosteronaemia.

Reform in personnel matters and the distribution system began to be implemented from October 2001 in line with the guiding principle of putting quality at the core of medical practice, taking doctors as the axis, putting emphasis on profits, and using distribution by merit as a lever. A series of reform measures was adopted: the employment of the entire staff on the basis of a contract system and transformation of identity management into post management; division of medical service units based on medical groups, with the attending doctors being in charge; and intra hospital distribution by the principle of more pay for more and better work. As a result, a management operating mechanism that specifies clear cut job responsibilities and combines responsibilities, powers and interests has been set up, bringing about good results.

ObjectiveTo investigate the effect of β-adrenoceptor (β-AR) blockers on G protein and heart function changes in rats with acute myocardial infarction (AMI) MethodsWistar rats with AMI induced by left anterior descending coronary branch ligation were randomly divided into compared with sham operation group. Eight weeks after therapy, hemodynamics was assessed by inserting catheters and the level of G protein was detected by Western blot analysis. ResultsCompared with the sham operation group, systolic blood pressure(SBP), diastolic blood pressure (DBP), left ventricular systolic pressure(LVSP) and left ventrieular pressure maximal rate of rise and fall(±dp/dtmax) in AMI group were significantly decreased, while left ventricular end diastolic pressure (LVEDP) and Gs and Gi protein levels were significantly increased (all P<0.05). Compared with AMI group, LVSP and ± dp/dtmax were increased, but LVEDP and Gi protein level were significantly decreased in metoprolol and carvedilol group. LVEDP and Gi protein level were decreased in carvedilol group compared with metoprolol group. ConclusionsCarvedilol can effectively suppress the change of G protein and improve the heart function after AMI, and the effect is better than that of metoprolol. This may be related with its β2-AR blocking effect.

Objective To investigate the effect of total flavonoid of Herba Pyrolae(TFHP) on the changes of ECG in rats with acute myocardial ischemia as well as the mechanism of the action.Methods Acute myocardial ischemic model was established by intravenous injection of pituitrin in rats,and ECG was recorded.Acute myocardial infarction model was established by the ligation of left descending coronary artery in rats,and serum level of nitric oxide(NO) and free fatty acid(FFA) was determined with spectrophotometrical method besides ECG.Results In comparison with the model controls,TFHP antagonized the decreased T wave by pituitrin and ligating coronary artery,and increased NO level and reduced FFA concentration in the serum of rats with acute myocardial ischemia.Conclusion TFHP protects the myocardium from ischemic injury possibly through increasing NO release and decreasing FFA production.

OBJECTIVETo study chemical constituents of leaves from Discocleidion rufescens.

METHODColumn chromatography and spectroscapic methods were used to isolate and identify the constituents.

RESULTFifteen compounds were isolated and identified as chrysophanol (1), physcione (2), taraxerol (3), beta-sitosterol (4), daucosterol (5), scopoletin (6), apigenin (7), acacetin-7-O-beta-D-glucoside (8), apigenin-7-O-beta-D-glucoside (9 ), luteolin (10), diosmetin-7-O-beta-D-glucoside (11), luteolin-7-O-beta-D-glucoside (12), gallic acid (13), amentoflavone (14) and myo-inositol (15).

CONCLUSIONCompounds 1, 2, 8, 11, 13 and 15 were isolated from the genus Discocleidion for the first time.

Euphorbiaceae ; chemistry ; Flavones ; analysis ; isolation & purification ; Plant Extracts ; analysis ; isolation & purification ; Sitosterols ; analysis ; isolation & purification

Objective:To investigate the predictive value of atherogenic index of plasma (AIP) in the assessment of acute pancreatitis (AP).Methods:598 patients diagnosed with AP admitted to the Affiliated Hospital of Yangzhou University between January 2016 and December 2020 were recruited and divided into severe acute pancreatitis group (SAP group, n=57) and non-severe acute pancreatitis group (non SAP group, n=541) according to the Atlanta Classification (2012 revision). General clinical data and related biochemical indicators of all enrolled patients were collected, and Bedside Index of Acute Pancreatitis Severity (BISAP) score, Ranson score and CT Severity Index (CTSI) score were performed. The risk factors of SAP were analyzed by logistic regression. Receiver operating characteristic (ROC) curve was used to analyze the evaluation value of AIP and various scoring systems on the severity of pancreatitis. Results:The AIP, white blood cell (WBC), neutrophil count (NEUT), fasting blood glucose (FBG), serum total cholesterol (TC) level, proportion of hyperlipidemia, proportion of diabetes, Ranson score, BISAP score, CTSI score of patients in SAP group were higher than those in non SAP group, and the difference was statistically significant (all P<0.05). Multivariate logistic regression analysis showed that AIP was an independent risk factor for SAP ( P<0.05). ROC curve showed that the are under the curve (AUC) of SAP predicted by AIP was 0.706(95% CI: 0.631-0.782, P<0.001). Conclusions:AIP is an independent risk factor for SAP, which helps to assess the severity of AP.