1.Development and Application of SMS Platform for Appointment Registration
Lei JI ; Junwen LV ; Wanguo XUE
Chinese Medical Equipment Journal 2003;0(12):-
Objective To resolve the problem of low speed and high failure rate of SMS.Methods The SMS module was separated,and the appointment registration system and SMS device were taken apart.Results The separate SMS platform for appointment registration system was established.Conclusion The response speed of the appointment registration system is enhanced greatly.
2.Epitope analysis of a novel Homo spains synapse associated protein(FRG4) and preparation of antibody
Ren WANG ; Xiangdong YANG ; Shunlin QU ; Junwen LIU ; Hongwei LV ; Zhijuan JI ; Shuli SHENG
Chinese Journal of Immunology 2001;0(07):-
Objective:To analyze a novel epitope of Homo sapiens synapse associated protein and synthesize polyclonal antibody.Methods:FRG4 full-length sequence was obtained by PCR from human fetal liver library;by bioinformatics to detect the second structure of amino acids encoded by FRG4 and its epitope and motifs;by solid-phase peptide synthesis method to synthesize FRG4 peptides,then peptides were immunized to rabbits;by immunohistory to detect the expression of FRG4 in HepG_2 cells.Results:Select 13-peptides PKLVKEEVFWRNY by bioinformatics to synthesize rabbit anti-human FRG4 polyclonal antibody.Antibody purity was 82.79% and antibody dilution was 1∶16 000 detected by ELISA.The antibody had a good reaction and speciality in Western blot,it was mainly expressed in cytoplasm of HepG_2 cells.Conclusion:A novel Homo sapiens synapse associated protein(FRG4) antibody was synthesized successfully.