Objective To evaluate the therapeutic efficacy of the comprehensive auricular point therapy in treating post-stroke urinary incontinence. Method A hundred patients with post-stoke urinary incontinence were randomized into a treatment group and a control group, 50 in each group. The treatment group was intervened by comprehensive auricular point therapy, while the control group was by rehabilitation training. The therapeutic efficacies were evaluated after 2 treatment courses. Result The total effective rate was 82.0%in the treatment group versus 62.0%in the control group, and the difference was statistically insignificant (P>0.05). Conclusion Comprehensive auricular point therapy is effective in treating post-stroke urinary incontinence.

Objective To explore the mechanisms of ischemia-reperfasion(I/R)injury of the bile duet and the effect of Atomolan on Isehemia-reperfusion injury of the bile duct in liver transplantation.Methods Pathological changes of the bile duct cells in three groups of rat liver transplantation model were observed through light microscope.And the effective protection of Atomolan against ischemia-repeffusion injury was observed.Results In ischemia-reperfusion group,the injury of bile duct cells were more serious,the level of ALT,AST and γ-glutamyhransferase elevated higher,oxygen free radical generated more,and the apoptosis of bile duct ceils appeared more,compared with Atomolan pretreatment.Conclusion The injury in bile duct cells is more serious than that in hepatoeyte in I/R injury.Atomolan can provide effective protection against liver Ischemia-reperfusion injury.The mechanisms may be through depress the production of oxygen free radical and decrease the apoptosis of bile duct cells.

Obiective To evaluate the value of allograft bone for acetab ular and femoral reconstruction in THA revision surgery. Methods A total of 19 p atients underwent reconstruction of acetabular and proximal femoral bone defects from June 1996 to December 2000 were reviewed. Eleven of the patients were men and 8 women with a mean age of 66.5 years and 63.2 years respectively at the t ime of resision surgery. According to AAOS system, the acetabular bone defects w ere classified into type I in 2 hips, type II 10 hips and type III 4 hips, and o n the femoral side, there were type I in 3 hips, type II 11 hips and type III 2 hips respectively. The reconstruction of bone defect of acetabulum included stru ctural allograft in 3 hips, impacted morselized allograft in 3 hips, and metal w ire mesh with morselized allograft in 3 hips. In all 3 of femoral segemental def ects, the reconstructions of the proximal femoral bone defects were done with st ructural allografts. Metalwire mesh with morselized allograft for femoral bone d efects were performed in 2 cases and morselized allogratts alone for femoral bon e defects in 4 cases; both acetabular and femoral bone defects were reconstruct ed with impacted morselized allografts in 6 cases (7 hips), and 4 patients with no bone grafts; Harris score system and radiograph were used for the final eva luation. Results Ninteen patients were followed up for an average period of 46 m onths (ranges, 6 to 68 months), Harris score improved from 42.7 points pre rev ision to 82.7 points at final follow up, the incorporation of allografts bone in 15 patients (16 hips) were noticed radiographically in all but one hip was fo und reabsorption of the structural allograft and radiolucents were found in the other 3 patients. No infection was encountered in this series. Conclusion If th e different types of bone defects were properly identified and suitable allogrft s adopted, reconstruction of bone defects with allografts during the THA revisi on surgery is a useful and reliable method. [

Objective To investigate the efficacy of allograft bone in posterior spinal fusion in scoliosis. Methods From March 1995 to March 2000, 60 patients with scoliosis, who underwent posterior spinal instrumentation and fusion using allograft or autograft bone, were evaluated prospectively. All of the patients were randomized into group A (30 cases using only allograft bone) and group B(30 cases using autograft iliac bone).Among the patients in group A, there were 16 with congenital scoliosis, 12 with idiopathic scoliosis, 1 with neurofibromatsis scoliosis and Marfan syndrome respectively. The average age at surgery was 14 years 6 months. the average preoperative major curve was 86.6? with an average number of 8 fused segments (range, 4 to 10 segments). Whereas in group B, there were 15 with congenital scoliosis, 9 with idiopathic scoliosis, 5 with neurofibromtasis scoliosis and 1 with Marfan syndrome. The average age at surgery was 13 years and 8 months. The average preoperative major curve was 77.8? with an average number of 7 fused segments (range, 5 to 10 segments). The mean operative duration, average blood loss, complications and loss of correction between the two groups were analyzed comparatively. Results Each patient had a minimum 2-year follow-up with an average duration of 4 years 5 months. Group A was better than group B by a shorter operative duration, less blood loss. At last follow-up, the major curve measured an average of 44? (range, 31? to 72?) in group A and 41? (range, 24? to 68?) in group B respectively. There were 3 (10%) and 2 (6.7%) pseudoarthrosis occurred in group A and group B respectively. One superficial infection developed in group A. There was no significant difference of the average loss of correction and complication rate between the 2 groups. But there were 6 patients who had donor site pain in group B. Conclusion A satisfactory outcome can be achieved in posterior spinal fusion in scoliosis using allograft bone especially when a large amount of bone graft is needed.

[Objective]To investigate the medium-term outcomes of surgical treatment of vertigo secondary to cervical spinal instability.[Method]From Oct.2000 to Jun.2006,28 patients underwent operation for vertigo secondary to cervical spinal instability and were followed prospectively.There were 21 females and 7 males with an average age of 42.5 years(rang,30 to 56 years).The involved levels included C3、4 in 12 cases,C4、5 in 8,combined C3、4 and C4、5 in 4,and combined C4、5 and C5、6 in 3.Surgical methods included anterior discectomy and fusion with interbody bone grafting and internal fixation with plate or fusion with cages.JOA score system was used for the evaluation of neurological function.[Result]All the patients were followed for 43 months(30 to 60 months).Satisfactory results were achieved in most of the patiens 3 months after surgery.At latest follow-up,complete relief of the preoperative complaints was obtained in 22 patients with incomplete relief in 4 and no obvious improvement in 2.Neurological function was improved from preoperative JOA 10.10 to postoperative JOA 14.90 with satistically significance.Adjacent unstable segments proximal to fuxion level were noted in three cases but with no recurrence of the preoperative symptoms.[Conclusion]For properly selected patients with vertigo secondary to unstable cervical spine,satisfactory medium-term clinical outcomes can be achieved appropriate surgical methods.

Objective:To investigate the duck thymocyte surface antigens.Methods:Repeated immunization of normal BALB/c mice with separated thymocyte from mature duck result in the production of mAbs which react with duck thymocyte surface antigens.Applying the immunohistochemistry assay,tracing the originating and distributing of lymphocytes which reacted with mAb in spleen,thymus,and bursa of duck different development stages.Results:Five monoclonal antibody-producing hybridomas(B6,B8,C12,E6,H5)were isolated.According to the result of immunohistochemistry assay,there were distributing strained lymphocytes in thymus,bursa and spleen of duck.The stained cells belonged to different populations.Conclusion:The mAb C12 reacted with the common cell surface marker of between mature T lymphocytes and dendritic cells.

Laiminin,which has been found 15 isoforms up to today,is a kind of noncollagen glycoprotein of extracellular matrix.However,the functions of laminin varis from each other,which mainly takes participation in the process of the cell adherence,migration,differentiation,growth,cell signaling and tissue repair in vivo.In recent years,some scholars have found that laminin plays an important role in angiogenesis.The purpose of this article is to summarize the role of laminin in angiogensis and sum up the current research progress.

ObjectiveTo investigate the value of cavity isolation method on left colorectal obstruction in the anastomosis isolation. Methods60 patients with left colorectal obstruction were randomly divided into two groups:ileostomy group and cavity isolation method group,30 patients for each. After left colon resection was completed, ileostomy anastomosis group used the traditional method for colorectal anastomosis, and protected the anastomosis by ileostomy. Cavity isolation method group protected the anastomosis by cavity isolation method. Then the indexes in the two groups,such as anastomotic height, operation time, anal exhaust time,incidence of anastomotic leakage and its complications,hospitalization time, medical cost and so on were analyzed. Results Longer postoperative hospital stay (9.3 + 4.2) d were observed in ileostomy group than those ( 15.6 + 3.3 ) d ( P ＜ 0.05 ). Earlier time of the first flatusand oral intake(87.2 ± 6.3 ) h, ( 91.2 ± 7.2 ) h were observed in ileostomy group than those ( 101.8 + 7.5 ) h,( 117.3 ± 8.6) h in cavity isolation method group ( all P ＜ 0.05 ). ConclusionThe anastomosis isolation was a safe and feasible new technology that could reduce the complications caused by anastomotic leakage, and it had the advantages of short hospital stay, low cost,less suffering ect.

Objective:To explore the relationship between the length of cervical spinous process and cervical motion and affected segment of cervical spondylotic myelopathy(CSM).Methods:Retrospective analysis was performed on 375 patients who underwent cervical surgical treatment due to single-segment cervical spondylotic myelopathy from January 2015 to January 2019. There were 200 males and 175 females, aged 50.72±9.39 (range 40 to 60) years. Several parameters, including the sagittal diameter of vertebral body, the sagittal diameter of cervical canal, the length of cervical spinous process, C 3-C 7 lordotic angle, range of motion (ROM) at C 3-C 7 and segmental ROM were measured via preoperative plain radiographs. All parameters were tested via Shapiro-Wilk method. Pearson correlation analyses was used to quantify the relationship between the lengths of C 3-C 7 spinous process and segmental ROMs. Receiver operating characteristic (ROC) curve was mapped to obtain the cut-off points according to the length of cervical spinous process which had significant differences. Patients were divided into two groups based on the cut-off points. χ2 test and t test were used to exclude the interference of age, gender and other anatomical factors and compare the differences in the affected segment of cervical spondylotic myelopathy between groups, so as to analyze the relationship between the length of cervical spinous process and affected segment of cervical spondylotic myelopathy. Results:There were significant differences of C 6 spinous process 27.82±6.01 mm and significantly negative correlation between the length of C 6 spinous process and the ROM at C 6,7 segment ( r=-0.338, P<0.001), while no significant correlations were found in other segments. ROC curves were mapped to obtain the cut-off points, and the cut-off point was 0.76. Group I: the ratio of the length of spinous process of C 6/C 7 (C 6/C 7 ratio, range 0.49 to 1.01) under 0.76, Group II: C 6/C 7 ratio more than 0.76. Compared with patients with longer-type C 6 spinous process (C 6/C 7 ratio ≥0.76), patients with shorter-type C 6 spinous process (C 6/C 7 ratio <0.76) had significantly bigger ROM at C 6,7 segment (10.11° vs 7.10°, P<0.001) and higher incidence of C 6,7 spinal cord compression ( χ2=16.642, P<0.001, OR=2.521), while differences in age, sex, sagittal diameters of vertebral body and spinal canal between two groups were not significant. Conclusion:The length of C 6 spinous process was significantly correlated with ROM at C 6,7 segment and the incidence of C 6,7 degenerative myelopathy. The length of C 6 spinous process can be considered as a predictor of development of C 6,7 degenerative myelopathy.

Objective To investigate the variations of tendency of drug-resistance from Jan 2006 to Dec 2008. the origin and the distributing of Acinetobacter baumannii and provide basis for selection of clinical drugs.Methods The characteriztion and sensitivity to antibiotics of Acinetobacter baumannii were analysed using VTTEK-32 system.Results 107 strains of Acinetobacter baumannii were separated and characterized during the three years.The rates of resistance to antibiotics were Imipenem 0.9%,Amikacin 5.6%,Ampicillin/Sulbactam 6.5%,Ampicillin 74.8% Ceftriaone 63.6%,Cefotaxime 38.3%,Aztreonam 25.2%.The resistance rate of Acinetobacter baumanii increased from 2006 to 2008,showed increasing tendency.Conclusion Acinetobacter baumannii showed good sensitivity to Carbapenems,Aminoglycosides,β-lactam antibiotics,Acinetobacter baumanii are widely distributing in hospital and are widely resistant to many kinds of antibiotics,The results suggested that the resistance of Acinetobacter baumannii to antibiotics should be continuously monitored,it will be beneficial for infection-control to collect antibiotics basing on the results of drug-sensitive test.