AIM　To study the effect of ginsenoside Rg1 on the learning and memory impairment in mice induced by aggregated β-AP(25-35). METHODS　Mice were administered Rg1 (5, 10 mg*kg-1, ip) for 10 d and control mice received daily ip injections of saline after the intracerebroventricular injection of aggregated β-AP(25-35). After the final treatment, passive avoidance and performance in the Morris water maze (MWM) were assessed. and the activity of cortical and hippocampal ChAT and AchE were detected after the final behavior test. RESULTS　Ginsenoside Rg1 (5, 10 mg*kg-1, ip) significantly ameliorated the learning and memory impairment induced by β-AP(25-35). Rg1 (5, 10 mg*kg-1) decreased the latencies and swim distances of mice to reach a hidden platform and improved the corresponding changes in search strategies occurred in the Morris water maze, and Rg1 (10 mg*kg-1, ip), increased step-through latencies also. Biochemical analysis showed that Rg1 (5, 10 mg*kg-1, ip) prevented the cortical and hippocampal ChAT activity decline induced by β- AP(25-35), and showed inhibition of the activity of AchE, although β-AP(25-35) showed no effect on the cortical and hippocampal AchE activity. CONCLUSION　These data showed that ginsenoside Rg1 significantly improved the learning and memory impairment induced by β-AP(25-35), and this effect could be attibuted to its inhibition of AchE and increase of ChAT activity.

Objective To study the protective effect of(-)clausenamide on the damage of PC12 cells induced by serum deprivation and to explore its related mechanism. Methods The cell viability was detected by MTT assay and morphological observation. The effect of(-)clausenamide on the PC12 cell apoptosis was analyzed by flow cytometry. Then western blotting and confocal microscope was used for the further study of effect of(-)clausenamide on the protein expression of GSK-3?,Bax and Bcl-2. Results(-)Clausenamide remarkably increased PC12 cell survival rate through inhibiting the PC12 cell apoptosis induced by serum deprivation at the concentration of 1 or 10 ? mol/L(P

Aim To study the effect of synapsinⅠon synaptic transmission in rat dentate gyrus induced by(-) clausenamide.Methods The basal synaptic transmission experiment was conducted through electrophysiological recordings.The effect of(-) clausenamide on synapsinⅠ phosphorylation was measured by western blot and confocal microscopy.Results(-)Clausenamide increased the population spike(PS) of hippocampal dentate gyrus.The phosphorylation of synapsinⅠ was increased both in cortex and hippocampus,the maximum effect was observed at 5 min in hippocampus and at 15 min in cortex.Furthermore,(-)clausenamide promoted the phosphorylation of synapsinⅠat a dose-denpendent manner in PC12 cells.The phosphorylation of synapsinⅠ in PC12 cells and synaptosomes incubated with(-)clausenamide was increased and reached maximum at 1~2 min.However,H89,PKA inhibitor,blocked the effect of(-)clausenamide on synapsinⅠ phosphorylation.Conclusion(-)Clausenamide activated synapsinⅠ via PKA signal pathway,which may contribute to the effect of(-)clausenamide on potentiating basal synaptic transmission.

Objective To observe the effects of acute stress on reproductive- endocrine function in female adult rats. Methods Acute immobilization stress was used to challenge the female adult rats and then the serum levels of progesterone (P), estradiol (E2) and corticosterone were measured 2 h after the stress.Results Acute stress significantly reduced E2 levels in each period of sexual cycle and increased the levels of P and corticosterone in the stressed rats.In addition, the ratio of estradiol and progesterone was disordered in stressed rats.Conclusion Acute stress can cause the disorder of estradiol and progesterone.Since stress is a major factor contributing to reproductive- endocrine disorder, this model will be useful for the research and development of drugs for reproductive- endocrine disorder. 

Objective To observe the effects of repeated immobilization on rats ovarian function and to observe the changes of ovarian function in menopause animals.Methods Daily vaginal smears were permfored to follow the estrous cycle in adult female rats.The animals with regular cycle were exposed to the scheduled daily repeated immobilization for 15-day,which included 5 stress episodes with each episode consisting of three immobilizations (one time per day). The duration of immobilization was progressively increased with the subsequent episode. Serum levels of estradiol (E2) and progesterone (P) were measured throughout the estrous cycle before and after the stress.Results The repeated stress resulted in the disruption of the estrous cycle characterized by longer phase of diestrus or by fewer epithelium from vagina in proestrus and estrus.In the stressed rats, a significant decrease of serum E2 and an increase of P were shown throughout the cycle compared to that before stress;the normal secretion cycle was interrupted or even disappeared; changes of serum P level in some rats were similar to the menopause animals.Conclusion Repeated immobilization can cause the disruption or degeneration of ovarian function,indicating that rats with stress-induced ovarian dysfunction is an appropriate animal model for testing protective effects of agents against aging and for the diseases related to ovarian dysfunction.

Objective To study the effects of quercetin (Que) on the release of endothelin-1(ET-1) and prosta cylin(PGI2) by normal human vascular endothelial cell(VEC). Methods Radioimmunoassay(RIA) was used to assess the amount of ET-1 and PGI2 produced by VEC. VEC proliferation was assessed by tetrazolium(MTT) assay. Results Que increased the normal VEC proliferation at the concentration of 5, 20, 40, 80, 100μmol/L and increased the pro duction of PGI2 and inhibits the release of ET by the normal VEC at the concentration of 5, 20 and 80μmol/L. Que at the concentration of 5, 20 and 80μmol/L had no direct effect on morphology of the normal VEC. Conclusion Que can stimulate the proliferation of VEC and inhibit the release of ET-1 and increase the formation of PGI2. The data sug gest that Que might be beneficial for the prevention and treatment of vascular endothelial injury-related cardiovascu lar diseases, such as atherosclerosis and thromboembolism diseases.

Objective To investigate the clinical results of end-to-end invagination pancreaticojejunostomy.end-to-end invagination pancreaticojejunostomy and duct-to-mucosa pancreaticojejunostomy of pancreaticoduodencetomy of patients with pancreatic and periampullary carcinoma. Methods Fifty-two patients underwent different pancreaticoduodenectomy in our hospital from August 1995 to July 2006 were collected and analyzed retrospectively.The incidence of pancreatic leakage,operation time,the blood loss and the postoperative complications were analyzed. Results Twenty-five patients received end-to-end invagination pancreaticojejunostomy.Sixteen patients received end-toside invagination pancreaticojejunostomy.Eleven patients received end-to-side pancreaticojejunal mucosa-mucosa anastomosis.The operation time of the duct-to-mucosa pancreaticojejunostomy was shorter than the two of the others(4 ～7.15 h).The perioperative comphcations occurred in 18 cases and the long-term postoperative complications occurred in 10 cases.The average length of stay in hospital was(19.32 ±5.45)days.Two cases died during perioperative period. Conclusion End-to-eed invagination pancreaticojejunostomy,end-to-side invagination pancreaticojejunostomy and end-to-side pancreaticojejunal mucosa-mucosa anastomosis are all common anastomosis methods.Pancreatic leakage is a serious postoperative complication.The selection of anastomosis method is based on the condition during operation.

Objective To compare the distribution of collagen type Ⅲ and Ⅳ in mucosa of normal and carcinoma cardiac tissues and to analyze the influence of changed collagen on auto-fluorescence after canceration.Methods Using immunohistochemical technique with monoclonal anti-collagen typeⅢ and Ⅳ,the difference of distribution in extracellular matrix of two types collagen between normal and carcinoma tissues from 16 patients were measured with semi-quantitative score methods.Results In normal mucosa,the epithelial basement membrane showed an intense positive reaction for monoclonal anti-collagen Ⅳ and an intensively stained ring band was formed around the grand basement membrane.The collagen type Ⅲ formed ring-like structures apparently representative of fibriform collagen immediately adjacent to the epithelial basement membrane.In carcinomatous mucosa,immunoreaction of the carinomatous cell nest was negative,and only remnants of positively were seen at the site of the former membrane by collagen type Ⅳ.The change of collagen type Ⅲ was not apparent and the positive reaction in carcinomatous stroma was more intensity than in normal stroma.There was significant difference in distribution of collagen type Ⅲ and Ⅳ between the normal and carcinomatous tissues(P

AIM To study the effects of quercetin (Que) on cell cycle and nitric oxide in H2O2-induced the human umbilical vein endothelial cell line(ECV- 304). METHODS The experiment were performed in culture of H2O2-induced human umbilical vein endothelial cell line(ECV-304) in vitro. Cell viability was assessed by MTT assay and cell cycle was observed by flow cytometry. Nitroxide(NO) of ECV304 was monitored as No2- with colorimetry. RESULTS H2O2 inhabited the ECV-304 prolifteration. Preincubation of ECV-304 with one for 24 h before H2O2 exposure significantly increased the Cell viability and S-phase and G2M-phase cells. one reduced lactate dehydrogenase(LDH) and increased the level of nitric oxide in H2O2-induced ECV-304. CONCLUSION These results demonstrate that one can produce the protective action on H2O2-induced cultured ECV-304 and its effect of action may be related to level of nitric oxide.

AIM　To study the effects of quercetin (Que) on cell cycle and nitric oxide in H2O2-induced the human umbilical vein endothelial cell line(ECV-304). METHODS　The experiment were performed in culture of H2O2-induced human umbilical vein endothelial cell line(ECV-304) in vitro. Cell viability was assessed by MTT assay and cell cycle was observed by flow cytometry. Nitroxide(NO) of ECV-304 was monitored as NO2- with colorimetry. RESULTS　H2O2 inhabited the ECV-304 prolifteration. Preincubation of ECV-304 with Que for 24 h before H2O2 exposure significantly increased the cell viability and S-phase and G2M-phase cells. Que reduced lactate dehydrogenase(LDH) and increased the level of nitric oxide in H2O2-induced ECV-304. CONCLUSION　These results demonstrate that Que can produce the protective action on H2O2-induced cultured ECV-304 and its effect of action may be related to level of nitric oxide.