Nerve growth factor, a kind of neurotrophic factor, plays an important role in neuronal development, differentiation, survival and neurogenesis, and is considered as a link between neuroendocrine system and immune system in asthma attack. The possible mechanism of effects of nerve growth factor on asthma is as follows: (1) nerve growth factor changes airway innervation, and facilitates the synthesis and release of neurotransmitter in nerve terminal, which will contribute to airway remodeling; (2) nerve growth factor induces eosinophils aggregation, proliferation and releasing inflammatory factor, which will lead to the abnormality of immunologic response; (3) nerve growth factor triggers the redundancy of adrenal medullary cells, which results in adrenal medullary cell to nerve cell transition, and then the impairment of chromaffin cell endocrine secretion function. As a result, the concentrations of adrenaline in circulation are not competent to relieve the bronchoconstriction in asthmatic attack.

Objective To investigate the possible mechanism of the biologic ethology effects of NGF and SP(substance P)on primary cultured adrenaline medullary chromaffin cells(AMCC).Methods To establish primary cultured AMCC by means of enzyme digestion and purify the cells by means of isopycnic gradient centrifugation and differential plating.To observe the morphological and ultrastructural changes after the addition of NGF and SP,and detect the concentration of adrenaline and noradrenaline in serum by ELISA.Results Confervaceous processes could be observed after 2 d of addition of NGF to the culture and the processes strenched longer as days went by the observation of electron microscopy.there are some drumstick-like and villiform processes in the cell membrane and some vesiculation be observed near the cell membrane of the primary cultured AMCC cells after the addition of NGF.The bioblast was abundant but the structure was not clear in the intracytoplasm and the concentration of adrenaline were decreased(P

Objective To find an efficient plan for the arrangement of the beds in hospital so that the beds-operating efficiency can be raised and the requirement of patients can be perfectly met. Methods According to the data analyzed via the method of mathematical statistics, optimizing model was built and automatic stimulation program was generated as well as conducted by matlab software. Results The characteristics of bed-occupation condition was acquired by data analysis, the result of optimizing model was stimulated by program and the most efficient plan emerges which was obviously better than the original FCFS (First Come First Serve) Plan. Conclusion The mathematical model built up is of great maneuverability in the practical situation to ameliorate the management efficiency of hospital beds considerably.

Aim To synthesize and identify artificial antigen of podophyllotoxin for the production of podophyllotoxin polyclonal antibody. Methods The hapten was synthesized by two different chemical approaches and characterized by TLC, IR, NMR, and MS. Mixed anhydride reaction (MAR) and active ester method (AEM) were used to couple the podophyllotoxin to carrier proteins (BSA and OVA). Characterization of artificial antigens was done by using spectroscopy and electrophoresis. The anti-podophyllotoxin polyclonal antibodies were obtained through immunizing rabbits. Results The results from IR, NMR and MS showed that 4-O-succinoyl podophyllotoxin (hapten) was successfully synthesized. The coupling molar ratios of the hapten and carrier proteins were 88.6 for Hapten-BSA1, 40.3 for Hapten-BSA2, 17.8 for Hapten-OVA1, and 54.2 for Hapten-OVA2. Hapten conjugates coupled with BSA yielded two sets of the specific and affinitive polyclonal antibodies. One set of antibodies showed an IC50 value of 2.21 μg·mL -1 with a detection limit of 0.12 μg·mL -1. Conclusion Antigenic conjugates were artificially synthesized, and based on these artificial antigens, polyclonal antibodies against podophyllotoxin were raised from rabbits immunized with two different immunogens and characterized with an indirect ELISA format.

Forty-eight men with normal glucose tolerance were divided into fatty liver disease ( NAFLD, n =23 ) and non-NAFLD (n =25 ) groups. The blood glucose excursion was evaluated by continuous glucose monitoring system. The results showed that the mean amplitude of glucose excursion[MAGE, (2. 17± 1.13 vs 1.45±0. 42 )mmol/L]and standard deviation of blood glucose[SDBG, (0. 88 ±0. 45 vs 0. 61 ±0. 21 ) mmol/L]were significantly higher in NAFLD group than in non-NAFLD group( both P＜0. 05 ). MAGE and SDBG were positively correlated with body mass index, waist circumference, and the increased value of plasma glucose 0. 5 h after glucose loading( △G30,all P＜0. 05 ). In multiple regression analysis, △G30, waist circumference, and age were significant independent predictors for MAGE( P＜0. 05 or P＜0. 01 ). △G30 and waist circumference were significant independent predictors for SDBG( P＜0. 05 or P＜0. 01 ).

Objective To investigate the effect of video -assisted thoracoscopic surgery (VATS)and conventional thoracotomy in treatment of thoracic esophagus cancer,to provide the reference for clinical.Methods 90 cases of thoracic esophageal cancer in our hospital from January 2012 to January 2015 were chosen as the research subjects.They were randomly divided into observation group(application of VATS treatment)and the control group (application of traditional open chest surgery).The quantity difference,chest drainage,hospitalization time,complica-tions,recovery conditions and other indicators of bleeding were compared in the two groups.Results The operation time of the observation group[(267.6 ±76.5)min]was shorter than that of the control group[(324.4 ±87.6)min]. The amount of intraoperative bleeding[(235.3 ±79.5)mL],drainage volume[(327.5 ±95.2)mL]of the observation group were less than the control group[(398.2 ±98.3)mL and (752.6 ±156.4)mL].Postoperative hospitalization time[(12.2 ±3.2)d]of the observation group was shorter than the control group[(15.8 ±4.4)d].The differences were statistically significant (t =3.276,8.644,15.575,4.439,all P <0.05).There were no significant differences between two groups in recurrence and metastasis rate,mortality rate and total survival rate (χ2 =0.123,0.212, 0.212,all P >0.05).The complication rate of the observation group was lower than that of the control group,the difference was statistically significant (χ2 =4.865,P <0.05).Conclusion In the treatment of thoracic esophageal carcinoma,VATS and conventional thoracotomy surgery has good effect,and VATS has small injury,less complication.

BACKGROUNDThere is still disadvantage in animal model for lung cancer study, no matter what is xenograft nude mice or rats/mice lung neoplasm induced by carcinogenesis.The purpose of the current investigation is to explore a simple and convenient and reliable method for lung neoplasm animal model.

METHODSThe prepared 36 female Sprague Dawley (SD) rats, with the range from 180 to 220g of body weight, were randomly divided into two groups, each group included 18 rats. In the study group, the rats were given 2mg of 3, 4-benzopyrene soluting in 0.2mL corn oil every two-weekly pulmonary injection for 4 times through right middle-chest percutaneous puncture under control of anaesthesia by pentobarbital sodium i.p. The controls were only given 0.2mL corn oil injection simultaneously. The rats were sacrificed after suffering from dyspnea, and the survived rats were sacrificed at narcotism in 1 year after the first 3, 4-benzopyrene toxicosis. Lung, brain, liver, esophagus and stomach of all rates were anatomized in search of tumor.

RESULTSNo lung neoplasm was found in the control group within 1-year observation. The earliest dyspnea caused by lung malignant neoplasm was observed in 16 week after the first injection in a rat treated with 3, 4-benzopyrene oil, then 10 rats were sacrificed and found with malignant neoplasm in theirs right lung when the rats suffered from dyspnea, and 1 rat was found a huge tumour in its right lower limb in 26 weeks after treatment. The other 6 rats were sacrificed in 1 year after the first 3, 4-benzopyrene oil treatment, in which 1 rat was found with a malignant neoplasm in its right lung and 5 rats were normal. No tumor was found in brain, liver, esophagus and stomach. Out of these 18 rats, 12 (66.67%) lung malignant neoplasms and 1 limb malignant neoplasm were found within 1 year in the experimental group which yielded a total cancerogenic rate of 72.22% (13/18). The lung neoplasms included: 2 poor-differentitaed squamous cell carcinomas, 3 adenocarcinomas and 7 undifferentiated carcinomas.

CONCLUSIONSThe results suggest that 3,4-benzopyrene pulmonary injection by percutaneous puncture may provide an efficiency method for lung neoplasm model established in rats.

Objective To explore the short-term and long-term efficacy of anterior cervical dis-cectomy and fusion(ACDF)under microscopic assistance for patients with focal ossification of the posterior longitudinal ligament(OPLL).Methods A total of 105 patients with focal OPLL treated between February 2020 and February 2022 were retrospectively selected.They were divided into mi-croscope group(n=56)and conventional group(n=49)based on whether ACDF was assisted by microscopy.Perioperative indicators,Visual Analogue Scale(VAS)scores,36-item Short-Form Health Survey(SF-36)scores,spinal canal cross-sectional area at the narrowest point,anteroposteri-or diameter of the spinal cord,cervical range of motion,intervertebral height,joint function[Japanese Orthopaedic Association(JOA)cervical spine disease score and Neck Disability Index(NDI)],and occurrence of complication were observed.Results The average operative time and average hospital stay in the microscope group were shorter than those in the conventional group[(141.85±18.35)min and(10.18±1.58)d versus(159.46±21.42)min and(12.03±1.82)d,P＜0.05].The average intraoperative blood loss in the microscope group was(80.65±7.28)mL,which was less than that(103.52±10.43)mL in the conventional group(P＜0.05).The VAS score and SF-36 score at six months postoperatively were better in the microscope group compared to the conventional group[(2.82±0.53)and(79.56±5.68)versus(3.51±0.60)and(72.35±4.77),respectively,P＜0.05].Spinal canal area and anteroposterior diameter of the spinal cord between the two groups postoperatively showed statistically significant differences[(138.24±24.32)mm versus(123.62±18.74)mm,(5.42±0.46)mm versus(4.73±0.51)mm,P＜0.05].The cervical range of mo-tion at six months postoperatively in the microscope group showed significant difference compared with that in the conventional group[(25.21±3.37)°versus(28.65±3.56)°,P＜0.05].The complication rate in the microscope group was lower than that in the conventional group(5.36％versus 18.37％,P＜0.05).Japanese orthopaedic association(JOA)and National Death Index(NDI)scores showed statistically significant between-group and time-point difference(P＜0.05).Conclusion Microscopic assistance during ACDF for focal OPLL provides a clear field of vision and facilitates effective decompression of the spinal cord by using a drill to remove and thin the ossified focus,thereby improving short-term pain and joint function while reducing the risk of spinal cord in-jury and other complications.

Objective To explore the short-term and long-term efficacy of anterior cervical dis-cectomy and fusion(ACDF)under microscopic assistance for patients with focal ossification of the posterior longitudinal ligament(OPLL).Methods A total of 105 patients with focal OPLL treated between February 2020 and February 2022 were retrospectively selected.They were divided into mi-croscope group(n=56)and conventional group(n=49)based on whether ACDF was assisted by microscopy.Perioperative indicators,Visual Analogue Scale(VAS)scores,36-item Short-Form Health Survey(SF-36)scores,spinal canal cross-sectional area at the narrowest point,anteroposteri-or diameter of the spinal cord,cervical range of motion,intervertebral height,joint function[Japanese Orthopaedic Association(JOA)cervical spine disease score and Neck Disability Index(NDI)],and occurrence of complication were observed.Results The average operative time and average hospital stay in the microscope group were shorter than those in the conventional group[(141.85±18.35)min and(10.18±1.58)d versus(159.46±21.42)min and(12.03±1.82)d,P＜0.05].The average intraoperative blood loss in the microscope group was(80.65±7.28)mL,which was less than that(103.52±10.43)mL in the conventional group(P＜0.05).The VAS score and SF-36 score at six months postoperatively were better in the microscope group compared to the conventional group[(2.82±0.53)and(79.56±5.68)versus(3.51±0.60)and(72.35±4.77),respectively,P＜0.05].Spinal canal area and anteroposterior diameter of the spinal cord between the two groups postoperatively showed statistically significant differences[(138.24±24.32)mm versus(123.62±18.74)mm,(5.42±0.46)mm versus(4.73±0.51)mm,P＜0.05].The cervical range of mo-tion at six months postoperatively in the microscope group showed significant difference compared with that in the conventional group[(25.21±3.37)°versus(28.65±3.56)°,P＜0.05].The complication rate in the microscope group was lower than that in the conventional group(5.36％versus 18.37％,P＜0.05).Japanese orthopaedic association(JOA)and National Death Index(NDI)scores showed statistically significant between-group and time-point difference(P＜0.05).Conclusion Microscopic assistance during ACDF for focal OPLL provides a clear field of vision and facilitates effective decompression of the spinal cord by using a drill to remove and thin the ossified focus,thereby improving short-term pain and joint function while reducing the risk of spinal cord in-jury and other complications.

OBJECTIVE: To investigate the effect of Dahuang Zhechong pills (DZ) on arterial thrombotic model in vivo. METHODS: Sixty-five rabbits were randomly divided into 7 groups: normal, model (collagen encapsulated thread-drawing),model+aspirin (ASA), model+clopidogrel (CP),model+ASA+CP, model+ low dosage DZ (DZL), and model+high dosage DZ (DZH). All rabbits except the normal group were fed with the drugs repectively for 8 days,and sacrificed at 2 hours after the last feeding, obtained aortae. The pathological changes in the aortae were observed under microscope,and the level of FDP, D-dimer and tissue factor (TF) were measured by enzyme-linked immunosorbent assay (ELISA). RESULTS: The vascular vessels were filled with thrombi in the model group and the elastic membranes of the vessel wall were seriously injured. The arterial thrombi were observed around the vascular wall in the DZL group, but some of the thrombi were dissolved. The number of thrombi was remarkably decreased in the DZH group, and most thrombi were dissolved and the vascular intimal membranes were intact. Compared with the model group, the dry and wet weight of the thrombi and the level of D-dimer, FDP, and TF in the plasma were significantly attenuated (P<0.01) in all the treatment groups. There were no significant difference between the DZL group and the ASA group in the dry weight, D-Dimer, and FDP (P>0.05). The pathological changes in the vascular vessel and the elevation of plasma parameters in the DZL group were similar to those in the ASA and CP groups (P>0.05). The dry and wet weight, D-dimer, FDP, and TF in the plasma in the DZH group were significantly lower than those in the DZL group (P<0.01 or P<0.05, separatively), and closed to those in the ASA+CP group. CONCLUSION Dahuang Zhechong pills are potential novel anti-thromobotic agent for arterial thrombosis.
Animals ; Carotid Artery, Common ; metabolism ; pathology ; Drugs, Chinese Herbal ; therapeutic use ; Fibrinolytic Agents ; therapeutic use ; Male ; Phytotherapy ; Rabbits ; Random Allocation ; Thromboplastin ; metabolism ; Thrombosis ; drug therapy