AIM: To establish a technical process for the purification of eleutheroside D from Acanthopanax Senticosus Harms. METHODS: D101, NKA-9, and AB-8 type of macroporous adsorbents were used to separate and purify eleutheroside D, and HPLC was adopted to analyse eleutheroside D purity and its yield. RESULTS: The static adsorption capacity of the three types of macroporous adsorbent were 11.88, 7.92 and 12.18mg?g -1, respectively. The static elution power were 70.1%, 55.3% and 93.8%, respectively. The biggest elution power was 92.6% by use of 30% alcohol and eleutherosides D purity was 27.4%, and as a process of purification, the purification temperature was controlled between 15℃～25℃. CONCLUSION: The AB-8 type macroporous adsorbents show better comprehensive adsorpion property. It could be used to purify eleutheroside D.