Objective:To observe the effect of emergency airway established by laryngeal mask for cerebral protection, and to popularize using laryngeal mask for establishing emergency airway in cardiopulmonary resuscitation(CPR) among paramedics.Methods:To compare the rescuing effect, 49 cases of patients with cardiac arrest were divided into 2 groups as different rescue method: laryngeal mask group (23 cases), emergency airway established by laryngeal mask airway(LMA); face mask group (26 cases).Results: After aerating 5 minutes, the average oxyhemoglobin saturation has recovered to 94.1% in laryngeal mask group, and it was 78% in face mask group; heartbeat recovery rate was 100% in laryngeal mask group, and face mask group was 84.62%, and there was no statistical significant (x2=2.94,P>0.05); breathing recovery rates were 86.96% and 19.23%, respectively, in laryngeal mask group and face mask group, and there was statistical significant (x2=19.772,P<0.05); consciousness recovery rates were 43.48% and 7.69%, respectively, between the two groups, and there was statistical significant (x2=6.627,P<0.05). Conclusion: Compared with the face mask group, to establish airway for cardiac arrest patient by using laryngeal mask airway(LMA) would got efficient results in improving anoxia in cerebral, increasing spontaneous breath rate and consciousness recovery rate.

Objective To explore an effective therapy for post-operative urine retention. Methods A total of 264cases were randomly recruited into a intervention group (135 cases) and a control group (129 cases). The intervention group was treated with acupuncture and acupoints massage, and the control group was treated with conventional therapy. Results The total effective rate of was 97.04% and 77.52% in the intervention group and the control group respectively, showing significant difference (χ2=28.657, P＜0.01); The average time for the first time urination of two groups was 24.38min and 35.41 min respectively, also showing significant difference (t= -14.552, P＜0.01). Conclusion Acupuncture and acupoints massage was an effective way for the treatment of post-operative urinary retention.

Objective To observe clinical effect of treating postoperative rectal cancer with chemotherapy and herbal enema.Methods 45 cases with postoperative rectal cancer were randomly divided into a control group and a treatment group.The control group was treated with conventional chemotherapy,and the treatment group was treated with chemotherapy,herbal enema and nursing.The short-term effect,adverse reaction and long-term effect were observed in both groups.Results The short-term effect in the treatment group was significantly higher than that of the control group(P＜0.05).The adverse reaction of the treatment group was markedly decreased.The three-year survival rate and recurrence rate of the two groups had a significant difierence (P＜0.05,P＜0.01).Conclusion The treatment of chemotherapy and herbal enema on postoperative rectal cancer was better than conventional chemotherapy

Objective To observe clinical effect of treating bedsore with myijinhuang powder. Methods 35 patients with bedsore were randomly divided into a control group and a treatment group. The treatment group was treated with ruyijinhuang powder, and the control group was treated with routine western medicine. The clinical effect was observed in both groups. Results The clinical total effective rate in the treatment group was significantly higher than that of the control group (P<0.05) . Conclusion The treatment of bedsore with ruyihuangjin powder has good effect.

Objective To observe the clinical efficacy of traditional Chinese medicine retention-enema in the treatment of ulcerative colitis. Methods 116 cases with ulcerative colitis were randomly divided into treatment group and control group, with58 cases in each group. The treatment group adopted the therapy of traditional Chinese medicine retention-enema after cleaning up the bowel, while the control group adopted the therapy of conventional Western Medicine retention-enema. Both of the two groups were treated for one course. Results The total effective rate of was 87% in the treatment group and 42.5% in the control group. The difference between the two groups was obvious (P＜0.05), with the treatment group being better than the control group. The retention time of medicine in intestinal tract was obviously prolonged in the treatment group. Conclusion The clinical efficacy of tradidonal Chinese medicine retention-enema treatment is obviously better than conventional Western medicine retention-enema treatment.

levels are elevated in patients with NAFL and correlated with HOMA-IR, suggesting RBP4 may participate in the development of IR and NAFL.

Objective To construct the prokaryotic expression vector bearing fusion gene NT4-ADNF-9 and lay foundation for further study on genetic therapy of neuraseusory deafness. Methods By means of asymmetrical prince/ template, double stranded eDNA of activity dependent neurotrophic factor-9 (ADNF-9) was obtained, which included restriction enzymes sites on the two extremities. ADNF-9 eDNA was ligated to the signal and leader peptides of nenrotrophin 4 (NT4), and the fusion gene was named NT4-ADNF-9. Then it was suheluned into prokaryotic expression vector pBV220, and called pBV220/ NT4-ADNF-9. Results Evidences of DNA sequence analysis and restrtction enzymes digestion showed that we recombined ADNF-9 eDNA to the 3'terminal of the signal and leader peptides of NT4, and the fusion gene was subcluned into pBV220 successfully. Bioactivity of the products was proved that it could support the cell survival and neurite growth in the primary cultures of dorsal root ganglia (DRG) of embryonic day-8 cbicken neurons as compared to the control. Conclusion Prokaryotic expression vector pBV220/NT4-ADNF-9 can be constructed successfully and the bioactivtty is satisfactory.

Objective To investigate the expressions and clinical significance of Ki-67,p53,and survivin in esophageal cancer and precancerosis.Methods The expressions of Ki-67,p53,and survivin proteins were detected by immunohistochemical staining in 40 normal esophageal mucosa,136 precancerosis (42 mild atypical hyperplasia,43 moderate atypical hyperplasia,and 51 severe atypical hyperplasia),and 68 esophageal cancer tissues.The correlation of three proteins expressed in esophageal carcinoma tissues was analyzed.Results The positive expression rate of Ki-67 was 0 (0/40)for normal epithelium,35.7％ (15/42) for mild dysplasia,51.2％ (22/43) for moderate dysplasia,74.5％ (38/51) for severe dysplasia,92.6％ (63/68) for squamous carcinoma,respectively.The positive expression rate of p53 protein was 0 (0/40) for normal epithelium,28.6％ (12/42) for mild dysplasia,46.5％ (20/43) for moderate dysplasia,52.9％ (27/51) for severe dysplasia,67.6％ (46/68) for squamous carcinoma,respectively.The positive expression rate of survivin protein was 0 (0/40) for normal epithelium,38.1％ (16/42) for mild dysplasia,55.8％ (24/43) for moderate dysplasia,64.7％ (33/51) for severe dysplasia,89.7％ (61/68) for squamous carcinoma,respectively.Rank correlation analysis showed that abnormal expressions of Ki-67,p53,and survivin were correlated significantly with the pathological grading of the lesions (r =0.637,0.454,0.590,P ＜0.01).The expressions of Ki-67,p53,and survivin were positively correlated in esophageal carcinoma (r =0.407,0.646,P ＜ 0.01).Conclusions The abnormal expressions of Ki67,p53,and survivin were associated with the processes of the esophageal canceration,and the joint detection with three parameters has important clinical value.

AIM:To investigate the expression of C/EBP homologous protein (CHOP) and its correlation with proliferative/apoptotic ratio (PAR) in colorectal adenoma-carcinoma sequence under the same genetic background .ME-THODS:Four kinds of tissue samples under the same genetic background from 23 patients, including normal colorectal tissue, adenoma with low-grade intraepithelial neoplasia , adenoma with high-grade intraepithelial neoplasia and colorectal adenocarcinoma samples , were collected .TUNEL method and Ki-67 immunohistochemistry were applied to determine the PAR.The expression of CHOP was detected by immunohistochemistry SABC method .RESULTS: ( 1 ) Under the same genetic background , the level of CHOP expression is significantly higher in colorectal adenocarcinoma than that in the ade -noma with high-grade intraepithelial neoplasia , the adenoma with low-grade intraepithelial neoplasia and the normal muco-sa.The level of CHOP expression was significantly higher in the adenoma with high -grade intraepithelial neoplasia than that in the adenoma with low-grade intraepithelial neoplasia and the normal mucosa .The level of CHOP expression was signifi-cantly higher in the adenoma with low-grade intraepithelial neoplasia than that in normal mucosa .(2) Under the same ge-netic background , PAR was significantly higher in the colorectal adenocarcinoma than that in the adenoma with high -grade intraepithelial neoplasia , the adenoma with low-grade intraepithelial neoplasia and the normal mucosa .PAR was significant-ly higher in the adenoma with high-grade intraepithelial neoplasia than that in the adenoma with low-grade intraepithelial neoplasia and the normal mucosa .PAR was significantly higher in the adenoma with low-grade intraepithelial neoplasia than that in the normal mucosa.(3) CHOP levels were positively correlated with PAR in the adenoma with low-grade intraepi-thelial neoplasia , adenoma with high-grade intraepithelial neoplasia and colorectal adenocarcinoma .CONCLUSION:CHOP expression and PAR continuously increased and positively correlated along the adenoma -carcinoma sequence , indica-ting that endoplasmic reticulum stress mediates the carcinogenesis of colorectal adenomas .

OBJECTIVE: To investigate the effect of IL-17 and IL-23 in the pathogenesis of allergic rhinitis(AR) and non. allergic rhinitis(NAR). METHOD: Selected 156 cases of patients with allergic rhinitis (AR group) and 59 cases of patients with non-allergic rhinitis (NAR group), 60 cases of healthy people (control group). All cases in AR group and NAR groups were evaluated by a visual analog scale (VAS) score of nasal symptoms. Collected peripheral blood and nasal secretions in all cases and then detected IL-17 and IL-23 expression levels. RESULT: There was no significant difference in VAS score of AR group and NAR group (P>0. 05). IL-17 and IL-23 levels of serum and nasal secretions in AR group and NAR group were both higher than control group, with a highly significant difference (P <0. 05). The research showed a clear correlation between expression of IL-17 and IL-23 both in serum and nasal secretions of AR group and NAR(P<0. 05). CONCLUSION IL-17 and IL-23 may be important cytokines and IL-23/IL-17 pathway may play a significant role in the pathogenesis of allergic rhinitis and non-allergic rhinitis.
Case-Control Studies ; Humans ; Interleukin-17 ; blood ; metabolism ; Interleukin-23 ; blood ; metabolism ; Nasal Mucosa ; metabolism ; Rhinitis ; blood ; metabolism ; Rhinitis, Allergic ; blood ; metabolism