Objective A retrospective analysis on papers of geriatric neurological diseases which were published in seven Chinese journals of core periodicals from 1992 year to 2001 year. Methods Ten years of Chinese Journal of Geriatrics, Chinese Journal of Neurology, Chinese Journal of Neurology and Psychiatry, Chinese Journal of Nervous and Mental Diseases, Journal of Clinical Neurology, Journal of Apoplexy and Nervous Diseases, Chinese Journal of Clinical Neurosciences were reviewed and all papers on nervous diseases in the elderly were statistically analyzed. Results In these 7 journals, 231 papers on topics of senile neurological diseases were published in the period of 10 years. Among them, 148(64.1%) papers were published in Chinese Journal of Geriatrics, 28 papers (12 1%) in Journal of Clinical Neurology and 25 papers(10 8%) in Chinese Journal of Nervous and Mental Diseases. In all papers of senile nervous diseases, the cerebrovascular diseases occupied the first place, totally 78 papers accounted for 33 8%; Alzheimer's disease took second place, with 51 papers, accounted for 22 1% and Parkinson's disease was third one, 29 papers, accounted for 12 5%. In the other items of senile neurological diseases, there were multiple system atrophy in the elderly, myasthenia graves in the elderly, epidemiological and clinical characteristics of senile depression, epilepsia in the elderly, facial nerve paralysis in the elderly, senile traumatic intracranial hematoma and severe head trauma, etc. Conclusions Chinese Journal of Geriatrics is main carrier for publishing the papers of senile neurological diseases. The main published articles were in following orders: cerebrovascular disease, Alzheimer's disease and Parkinson's disease. It indicates that these 3 diseases are the important risk illnesses for the health of the elderly.

Objective To analyze the references cited from Chinese Journal of Geriatrics from 1992 to 2001 year and to approach the regular patterns of references. Methods All references of scientificpapers printed in Chinese Journal of Geriatrics from 1992-2001 year were imputed into CP. All references of treatises, literature reviews and lectures were analyzed according to different languages, time distributions and cited half-life, etc. Results In recent 10 years the majority of references were in Chinese and English, accounting for 29.4% and 69.5%, respectively. The names of cited periodicals were much scattered, 335 periodicals in Chinese and 1 016 periodicals in English. The half-life of the value of information in Chinese and English periodicals was degraded by year. According to Bradford's law the nucleus of periodicals in Chinese was 5, which was similar to the nucleus of periodicals from 1982-1991 year. Chinese Journal of Geriatrics still took the first place in Chinese periodicals. The nucleus of periodicals in English was much changed as compared with nucleus of periodicals from 1992-2001 years. The journal "Circulation"occupied the first place. Conclusions It is easy to get all deliverable journal literature via the internet, so there was more broad collection of recent primaryjournal literature and secondarydatabase. Chinese Journal of Geriatrics took the first place in Chinese periodicals.

To improving the teaching quality of the pharmacology theory teaching,we adopt questionnaire to understand the students’ request of teaching method and teaching content,thus to elevate the teaching overall level by raising their ability to teach and study.

Objective To investigate the benefits and challenges of medical student volunteers'involvement in community diabetes education and to provide evidence for future development and suggestions.Methods Self-made questionnaire survey and interview were conducted among members of community health education volunteer in Southeast University.Data concerning basic group composition,major service contents,intention and gain,sources of motivation,knowledge acquisition and skill training were analyzed.Results 88.t04％ volunteers chose professional work as the kind of work they were most interested in,in contrast,69.56％ did nonprofessional work in reality.Initial intention of 66.30％ volunteers was ‘to beautify curriculum vitae'.‘Morality promotion' was taken as major gain by most volunteers (36.96％) while ‘morality promotion' was the initial intention of only 4.89％ volunteers.46.20％volunteers thought that their sources of motivation came from ‘ recognition of society and university'.Through training and voluntary service,it was evident that volunteers gained increased diabetes knowledge,stronger professional identity and confidence as well as deeper understanding of patients' needs,but there were real demand for training in education and clinical skills.Conclusions The introduction of medical student voluntary service into community diabetes education is a win-win cooperation,which can cultivate college students' high morality as well as strengthen the community health education team.However,we still face challenges in volunteer team building and management.

Objective To investigate the effect of sensory and motor nerve homogenates at different concentrations on the proliferation and osteogenesis differentiation of bone marrow mesenchymal stem cells (BMSCs) in rats.Methods The saphenous nerve and the muscle branch of the sciatic nerve in rats were extracted surgically as sensory and motor nerve tissues,respectively.The primary nerve homogenates (10 mg/mL) were prepared as per 10 mg tissue with 1 mL osteoblast inducing conditional media,and 10 times diluted after filtration purification to prepare sensory and motor nerve homogenates at concentration gradients of 1.0,0.1,0.01,0.001 and 0.0001 mg/mL.Cultivation GFP ± rat pups BMSCs in vitro were trained to P3 generation.The experiment was carried out in 3 groups.The sensory and motor nerve homogenates of 500 μL at the above 6 concentration gradients were added during cultivation respectively in the sensory nerve group (n =18) and the motor nerve group(n =18) while 500 μL of osteoblast inducing conditional media was added in the control group(n =3).Cell proliferation quantity detection and alkaline phosphatase (ALP) staining were used to assess the proliferation and differentiation of BMSCs after 14 days.Results According to the results of CCK-8,the cellular absorbance values at concentrations of 1.0 and 0.1 mg/mL homogenate in the sensory nerve group (1.957 ±0.065 and 1.751±0.073) were significantly greater than in the control group (1.145±0.087) while the cellular absorbance value at concentration of 10.0 mg/mL homogenate in the motor nerve group (0.304 ± 0.619) was significandy smaller than in the control group (1.145 ± 0.087) (P ＜ 0.05).According to the ALP staining,the amounts of cellular calcium nodules in the sensory and motor nerve groups (2.667 ± 0.816 and 3.000 ± 0.632,respectively) were significantly smaller than in the control group (11.833 ± 1.471) (P ＜ 0.05).Conclusion Sensory nerve homogenate is different from motor nerve homogenate in that it may promote proliferation of BMSCs and inhibit osteogenesis differentiation of BMSCs in a certain rage of concentrations.

Objective To investigate the effect of prevascularized tissue-engineered bone graft on regeneration of femoral bone defects in rats.Methods Models of femoral bone defect were created at the bilateral hind limbs of 20 healthy female 10 week-old rats which were divided into 2 even groups randomly (n =10).In group A,conventional tissue-engineered bone grafts were transplanted into the femoral bone defects;in group B,tissue-engineered bone grafts and vascular bundles were implanted into the femoral defects.At 1,4 and 8 weeks after operation,3 rats were sacrificed each time in each group to harvest samples.The remaining one in each group served as a spare animal.Regeneration of bone defects and degradation of scaffolds were assessed by radiologic modality and hematein eosin staining.Results At week 1,the new bone ratio (BV/TV) was 5.47％ ± 1.90％ in group A and 8.49％ ± 1.26％ in group B,showing no significant difference (P ＞ 0.05);at weeks 4 & 8,the BV/TV were 17.54％ ±2.04％ and 39.73％ ± 4.01％ in group A,significantly lower than those in group B (25.32％ ± 2.15％ and 53.22％ ± 2.94％) (P ＜ 0.05).At weeks 1 & 4,the scaffold degradation ratios (RSV/SV) were 97.33％ ± 2.52％ and 80.60％ ±4.00％,showing no significant differences from those in group B (95.67％ ±3.51％ and 75.22％ ±6.20％) (P ＞ 0.05).At week 8,the scaffold degradation ratio in group A (65.46％ ±4.51％) was significantly higher than that in group B (50.19％ ±4.91％) (P ＜ 0.05).At week 8,hematein eosin staining showed better integration of scaffolds with the femur,faster degradation of the interior scaffolds and greater osteogenetic activity in group B.Conclusion Prevascularization of tissue-engineered bone graft may increase new bone volume and scaffold degradation rate,promoting repair of femoral bone defects in rats.

Psoriasis is a common immune-mediated inflammatory skin disease with multiple genetic and environmental risk factors.Abnormal vascular hyperplasia,excessive proliferation of keratinocytes and infiltration of various immune cells are the main pathological features.MAPK signaling pathway is involved in the pathogenesis of psoriasis,and SPRED1 has been proved to be a ma-jor negative regulator of MAPK signaling pathway in many diseases.This article reviews the possible role of SPRED1 in the pathogene-sis of psoriasis,in order to provide new insights into the pathogenesis of psoriasis and new directions for the treatment.

Objective:To observe the impacts of different postures on the hemodynamics of lower extremity vein.Methods:In this single center non-randomized controlled study in 15 healthy female volunteers, the hemodynamic changes in the common femoral vein were detected by color Doppler ultrasound at 10 different postures: supine position, slope positions with bed end elevated at 15°, 30° and 45°, trapezoidal positions with bed end elevated at 15°, 30° and 45°, and positions with bed head elevated at 30°, 45° and 60°.Results:Different postures resulted in significant differences in the velocity of blood flow in the common femoral vein ( P<0.05), with slope position at 45°> slope position at 30°> slope position at 15° = trapezoidal position at 30°> trapezoidal position at 15° = trapezoidal position at 45°> supine position> position with bed head elevated at 30°> position with bed head elevated at 45° = position with bed head elevated at 60°. Conclusions:In the postures observed in this study, the slope position with bed end elevated at 45° can promote the most effectively the blood reflux in the lower extremity vein, the trapezoidal positions with bed end elevated may not facilitate the distal blood reflux in the lower extremity vein, and positions with bed head elevated may hinder the blood reflux in the lower extremity vein.

Objective:To understand the cognition of research integrity of in-service medical staff in a tertiary hospital in Xinjiang, provide possible reference for research integrity training.Methods:In March 2021, an electronic questionnaire survey was conducted to investigate the knowledge of scientific research integrity of in-service medical staff in a tertiary hospital in Xinjiang. T test and analysis of variance were used for univariate analysis, and multiple linear regression was used for multivariate analysis.Results:550 valid questionnaires were collected in total, and the overall awareness rate of scientific research integrity of in-service medical staff was less than 60%. Univariate analysis showed that there were differences in cognitive score of scientific research integrity among medical staff who participating in different scientific research activities ( P<0.05). Multivariate analysis showed that the educational level of the in-service medical staff, whether they participated in scientific research training, whether they had the qualifications of graduate student supervisor, the interaction between educational level and participation in scientific research training was statistically significant ( P<0.05). LSD test of educational level and participation in scientific research training showed that there was a statistically significant difference in educational level of participation in scientific research training ( P<0.01). Conclusions:The cognition of research integrity on in-service medical staff in a tertiary hospital in Xinjiang need to be enhanced, and their knowledge of research integrity need to be improved. Various measures should be carried out to strengthen the research integrity education and training, so as to improve the level of medical staff’s cognition of research integrity, and improve the medical staff’s attitudes of research integrity.

Objective To investigate the effects of Bushen Huoxue Fang kidney-supplementing and blood-activating formula(BSHXF)on Wnt/β-catenin signal pathway in stem cell of thin endometrium. Methods During the 7thto 9thday after ovulation,100 mg endometrial membrane samples of selected patients with thin endometrium were taken with disposable endometrial sampling device.After washing with saline,the samples was fixed in 4%polyformaldehyde and then separated to culture the endometrial stem cells,and drug serum was prepared.The cultured endometrium stem cells were divided into the control group,High,medium and low dose groups of kidney-supplementing and blood-activating formula (BSHXF)and Western medicine group.After intervention,β-catenin /TCF(T cytokine)and transcrip-tional activity were measured by using double Luciferase Report System;CD34/CD117 protein expression was examined with immunofluorescence; Oct-4 gene was examined with RT-PCR; and ABCG2 protein was measured by using Western blot.Results After transfecting the same dosage of plasmid,there was a significant difference among mid-dose BSHXF group, high-dose BSHXF group, Western medicine group,and control group(P<0.05).CD34/CD117, Oct-4 gene and ABCG2 protein had positive ex-pression in the endometrium stem cells.There was a significant difference in the expression of CD 34 pro-tein between BSHXF groups and control group(P<0.05).There was a significant difference between high-dose BSHXF group and Western medicine group(P<0.01).The expression of CD117 protein in the high-dose group was significantly different from that in the control group(P<0.05),and there was a significant difference between the Western medicine group and the control group(P<0.01).RT-PCR test showed that the expression rate of Oct-4mRNA were significantly different from the interventional groups and the control group(P<0.05).Western blot test showed that the expression rate of ABCG 2 protein in the interventional groups were significantly different from that in the control group(P<0.05). Conclusion Kidney-supplementing and blood-activating formula can increase the fluorescent activity of thin endometrial stem cells, thereby promote the activation of Wnt/β-catenin signal pathway, and in-crease the expression of CD34/CD117,OCT4 gene and ABCG2 protein.