Objective To preliminarily investigate the effect of high mobility group box 1 (HMGB1) in the pathogenesis of chronic obstructive pulmonary disease (COPD) by detecting the HMGB1 level in bronchoalveolar lavage fluid(BALF) of the COPD patients and analyzing the relation between HMGB1 and airway limitation .Methods The research subjects were divided into the COPD group (stable COPD ,20 cases) and control group[20 cases of upper airway cough syndrome(UACS)] .All subjects were performed the bronchoscopic examination as well as bronchoalveolar lavage .The total cell counting and percentage of neutrophilic granulocytes in BLAF were detected and compared between the two groups .The HMGB1 ,IL‐1βand TNF‐αlevels in BLAF were detected by using ELISA and compared between the two groups .The relation between HMGB1 with IL‐1βand TNF‐αand the rela‐tion between HMGB1 with pulmonary function(FEV1% predicted value) in the COPD group was analyzed .Results The total cell counting and percentage of neutrophilic granulocytes of BLAF in the COPD group were higher than those in the control group (P<0 .01) .The levels of HMGB1 ,IL‐1β,TNF‐αof BALF in the COPD group were higher than those in the control group(P<0 .01 , P<0 .05 ,P<0 .01) .In the COPD group ,the HMGB1 level in BALF was positively correlated with the IL‐1βand TNF‐αlevels(r=0 .79 ,P< 0 .01 ;r= 0 .48 ,P< 0 .05) ,while negatively related with the pulmonary function value(FEV1% predicted value ,r=-0 .70 ,P<0 .01) .Conclusion HMGB1 participates in and promotes the occurrence and development of COPD airway inflamma‐tion and the HMGB1 level in BALF is correlated with the severity of COPD .

[Objective]To discuss the relationship between partial upper limb edema in the cervical spondylosis patients and the decompression operation.[Method]Ten cervical spondylosis cases with partial upper limb edema were reviewed,including 7 cases of cervical spondylotic myelopathy,2 cases of cervical radiculopathy and 1 case of OPLL(ossification of the posterior longitudinal ligament).Seven cases were treated with subtotal vertebrectomy and anterior internal fixation,one with subtotal vertebrectomy and anterior discectomy,2 cases with posterior decompression and fusion.All the patients were available for follow-up to evaluate the decay of the edema.[Result]All the 10 patients' edema disappeared with the duration from one day to over half a year.The treatment of anterior surgery made patients' edema disappear quicker than that of posterior surgery.[Conclusion]The occurrence of the upper limb edema in the cervical spondylosis patients is related to do with the irritated sympathetic nerve.The edema can be reduced in short term after the decompression operation.

MicroRNAs(miRNAs) is a class of small endogenous noncoding RNAs with 22 nucleotides.They can negatively regulate gene expression by inhibiting target mRNAs′translation or degrading mRNAs .Up to now, it has been proved that miRNAs play an important role in cellular proliferation , apoptosis , cell differentiation , and embryonic development .In this article , mechanism and function of miRNAs , principal methodologies in the research of miRNAs and the latest development of miRNAs in early embryonic de -velopment are discussed .

Asbestos ; adverse effects ; Environmental Exposure ; GPI-Linked Proteins ; metabolism ; Humans ; Population Surveillance

Recent related researches pointed out that a series of adverse reactions may occur when homocysteine (Hcy) level exceeded normal range .More and more studies indicated that Hcy level abnormal elevating was an inde‐pendent risk factor for cardiovascular diseases .This article summarized its related research situation .

The function and contents of WeChat public account service in military hospitals were found out by in-vestigating the WeChat public account, which showed that WeChat public account service is provided in more and more military hospitals, but its function should be further expanded and its management should be strengthened.

Objective To discuss the application of DWI and DTI in diagnosis and evaluation of brain tumor. Methods Combining with literatures, the application of DWI and DTI in brain tumor diagnosis, differential diagnosis, tumors classi- fication, preoperative evaluation, operation plan establishment, follow-up role of treatment were reviewed and evaluated. Results The DWI and DTI were available to brain tumor diagnosis, differential diagnosis and tumor grade. The DTI could be clearly shown the influence of tumor with white matter quality, it was important in operation plan establishment. The combination of intra-operative MRI, functional MRI and DTI was valuable for brain tumor resection. In the meantime, the treatment response of tumor and the changes of treatment-induced white matter were accurately assessed by DTI. Conclu- sion With the MRI hardware and software technology development, the DWI and DTI imaging information can be provided important value in brain tumor pathological changes diagnosis, evaluation, therapeutic planning and post treatment assess- ment.

ObjectiveComparing gastric perforation repair with traditional contrast,to explore the feasibility and superiority of the laparoscopic gastric perforation repair.Methods68 cases were randomly divided into two groups for laparoscopic gastric perforation repair and traditional repair,then compared two groups of treatment.Results Both operations were successful ( including laparoscopic repair in 34 cases) and surgery time,blood loss,postoperative drainage,length of stay,and cosmetic results of the comparison.ConclusionCompared with the traditional open surgery,the laparoscopic surgery had less trauma,leas pain,faster recovery,shorter hospital stay,high efficacy and good cosmetic results and other advantages.

Objective To investigate the expression of α-smooth muscle actin (α-SMA)in patients with valvular atrial fibrillation and study the relationship betweenα-SMA and atrial fibrosis in patients with valvular atrial fibrillation (AF).Methods For this study we enrolled 84 consecutive patients with rheumatic heart disease who were to receive cardiac surgery.The patients were divided into AF group (AF,n=39)and sinus rhythm group (SR, n=45).Their clinical data including baseline demographics,routine laboratory test and echocardiographics were collected before surgery.The right atrial tissue (0 .3-0 .5 cm3 )was disserted during the surgery.Right atrial fibrosis was observed by Masson staining.The mRNA expression ofα-SMA in atrial tissue were determined by Real-time quantitative PCR.Western blot was used to measure the protein expression ofα-SMA in atrial tissue.Results The two groups did not significantly differ in sex ratio,age,blood pressure,blood biochemical indicators or other aspects of medical history (P>0.05).However,left and right atrium diameters in AF group were significantly larger than those in SR group (P<0 .05 ).Masson staining suggested that collagen volume fraction and collagen content were significantly higher in AF group than in SR group (P<0 .05 ).The mRNA and protein expressions ofα-SMA in right atrial tissue were obviously higher in AF group than in SR group (coefficients P<0 .05 ).The mRNA and protein expressions ofα-SMA from right atrial tissue in the 84 patients were positively correlated with collagen content (coefficients of 0.587 and 0.607;P=0.029,0.014,respectively).Conclusion There is significant atrial fibrosis in patients with valvular atrial fibrillation,which is closely related to up-regulated expression ofα-SMA gene.

BACKGROUND:In rabbit femur fracture model experiment, there are a few studies on whether quadriceps tendon wil affect the stability of the fixed device. OBJECTIVE:To analyze the effects of quadriceps tendon to the stability of external fixator in rabbit models of femur fracture. METHODS:The 48 New Zealand white rabbits were randomly divided into two groups. External fixator was used to make fracture model. In the experiment group, quadriceps tendon was cut. In the control group, quadriceps tendon was retained. 2 weeks later, X-ray examination was utilized to observe the number of failed external fixator in both groups from modeling to success. RESULTS AND CONCLUSION:(1) X-ray examination:2 weeks after model establishment, external fixation frame screw was free from femur in one rabbit of the experiment group, and seven rabbits in the control group. Of them, one screw was broken. Threaded portion retained in the femur. (2) External fixation failure rate:failed external fixator was found in one rabbit in the experiment group, resulting in a failure rate of 4%, and 12 rabbits in the control group, resulting in a failure rate of 50%(P<0.05). These findings suggested that cutting off the rabbit quadriceps tendon can effectively ensure the stability of the external fixator, and indirectly improve success rate of the experiment.