Objective To discuss the value of minimum systolic and diastolic peak velocity ratio(S/D) and pulse index(PI) of the umbilical artery of pregnant twins in predicting perinatal outcome.Methods Pulsed Doppler measurements of the umbilical artery were conducted in 77 cases of twin pregnancise. Perinatal outcome was compared between normal and abnormal umbilical artery S/D,PI group respectively. The rates of abnormal umbilical artery S/D,PI in monochorionic and dichorionicdich were compared. Results The rates of adverse perinatal outcome,small for gestational age(SGA),structural malformation in abnormal umbilical artery S/D group were significantly higher than those in normal umbilical artery S/D group ( P

Objective: To determine whether aminoguaidine（AG）,a selective inhibitor of inducible nitric oxide synthase (NOS), affect the embroy development of mice, and to study the mechanisms of iNOS affecting on pregnancy. Methods: AG(10 mg*kg－1*d－1 or 20 mg*kg－1*d－1) were injected subcutaneously to mice from day 3，7，14. kill mice at day19. The weight of fetus and placenta, the number of fetus and fetal resorptions were measured. Histological changes of placenta and umbilical cord were observed. Immunohistochemistry and NADPH histochemistry were adopted to study NOS activity of placental and umbilical cord respectively. Results: The conceptus in the uterus were resorbed in the early pregnancy. In the early and middle pregnancy the weight of pregnant mouse and the number of fetus of AG groups was decreased. The number of fetal resorptions was increased. But the weight of fetus and placenta were not affected. The expression of iNOS in the placenta were no difference significantly between the two groups with image analysis. Conclusion: AG inhibits the implantation and growth of embryo in the early and middle pregnancy, buy doesn't affect the formed fetus and placenta significantly.

Objective To observe the inhibitory effect of xiaoaiping injection and octreotide on H22 tumor-bearing mice and find the best drug concentration,then to explore its mechanism.Methods Establish a mouse H22 subcutaneous tumor model.After tumor the experiment animals were divided into normal control group,model group,Xiaoaiping low,medium and high dose group,octreotide group,and the group of XAP low,medium and high dose groups were combined with OCT.Calculate the tumor's volume and draw the tumor growth curve.Intraperitoneal injection for 14 days,Inhibitory rate was calculated; To observe its pathological changes by light microscope; The ratio.of CD3 + NK1.1-T cells,CD3-NK1.1 + NKcells,CD3 + NK1.1 + NK-Tcells in peripheral blood were measured by flow cytometry.Results Compared with the control group,H22 liver cancer in different treatment group had a certain inhibition effect on growth,The inhibitory effect of the combination group was better than single-agent group,High-dose Xiaoaiping + octreotide was best,Tumor model group compared with normal control group,The ratio of T cells,NK cells and NKT cells was significantly lower(P ＜0.05) ; T cells,NK cells and NKT cells after treatment in each group had some enhancement,High-dose Xiaoaiping + octreotide was the most obvious,the ratio of T cells,NK cells and NKT cells of the combination group was significantly more than the single-drug group and the same concentration of octreotide monotherapy Xiaoaiping group(P ＜ 0.05).Conclusion High-dose Xiaoaiping + octreotide is the best drug for the inhibitory drug concentration.The inhibition of tumor growth may pass to improve the tumorbearing mice with immune status and enhance the body's anti-tumor capacity.

Objective To evaluate which parameters of transvaginal color Doppler ultrasonography(TVCDU) can effectively reflect blood flow in ovarian tumors. Methods TVCDU was preoperatively used to determine all the parameters of blood flow in 60 women with epithelial ovarian cancer(EOC) and 36 with benign ovarian cystadenomas (control group). Microvessel density(MVD) was tested in the tissue samples by immunohistochemical method(SP method). The parameters of TVCDU in EOC were compared with those in control group.The correlation between MVD and the parameters in EOC group and control group was analyzed. Results ①Statistical differences of arteria showing rate, arteria number, vasculature type, diastolic notch, PI, RI, S/D, TAMXV were seen between EOC group and control group(P

Objective To determine the levels of insulin-like growth factor binding protein-2 (IGFBP-2) in glioma tumor tissue and their clinical significance. Methods The levels of IGFBP-2 in glioma samples from 45 patients were detected with immunohistochemistry. Their correlation with tumor grade, p53 and Ki67 expression levels and the outcome of patients were tested. Results IGFBP-2 increased in glioma tissue in a grade-dependent manner, and significantly correlated with p53 and Ki67 expression. High IGFBP-2 level is significantly associated with earlier tumor recurrence and shorter overall survival. Conclusion Tumor IGFBP-2 levels can supply more prognostic information to the tumor grading system.

Objective To explore the clinical characteristics of Henoch-Sch(o)nlein purpura complicated with acute necrotizing acute pancreatitis,in order to provide information for the diagnosis and differentiation of Henoch-Scht(o)nlein purpura complicated with acute abdominal disease.Methods There was a case present with purpura,abdominal pain,massive ascites and poor spirit in Department of Pediatrics,the First Affiliated Hospital of Zhengzhou University.The clinical manifestations and physical examination results were summarized and discussed,some pediatric specialists from nephrology department,surgery department,digestive system department were invited to discuss the case.The treatment was adjusted according to result of discussion,the final diagnosis was tracked.Results The case was firstly diagnosed with Henoch-Sch(o)nlein purpura,acute diffuse peritonitis with cause in dispute.After the discussion,the intestinal necrosis,perforation could not be excluded,acute pancreatitis required further identification.Exploratory laparotomy was received because of the changing condition after discussion.The intraoperative diagnosis was acute necrotizing pancreatitis.The patient recovered well with medical treatment.Conclusion When children with Henoch-Sch(o)nlein purpura had an acute abdominal disease,acute necrotizing pancreatitis should be considered in addition to intestinal necrosis,perforation.

Objective To detect the expression and the biological activity of a recombinant adenovirus expression vector carrying human neurotrophin-3 (NT-3) receptor TrkC gene (Adeno-TrkC) in neural stem cells. Methods The expression of TrkC mRNA in 293 cells infected with Adeno-TrkC was detected by RT-PCR, and the expression of TrkC protein in neural stem cells infected by Adeno-TrkC was detected with immunocytochemistry and Western blotting. The effects of human neurotrophin_3 (NT-3) on the neural stem cells infected by Adeno-TrkC differentiating into neuron-like cells and astrocyte-like cells in vitro were observed. Results The transcription of TrkC mRNA and the expression of TrkC protein was detected in 293 cells and neural stem cells infected by Adeno-TrkC. This kind of TrkC was able to make more neural stem cells differenting into neuron-like cells in vitro with its ligand NT-3 and the percentage of neuron-like cell’s differentiation was 55^2% which was higher than that of other control groups.Conclusion TrkC protein is expressed in neural stem cells transfected with Adeno-TrkC and is able to make neural stem cells differenting into neuron-like cells. The present study may provide the basis on gene therapy of central nervous injury using further NT-3 and TrkC.

OBJECTIVE: To investigate the effect of plasma eardrum perforation homochronous with palatoplasty on hearing and tympanic cavity pressure in cleft palate children with secretory otitis media. METHOD: Fifty-six cases of cleft palate children with secretory otitis media were randomly divided into observation group and control group. For the control group, palatoplasty was carried out alone. For the observation group, homochronous with palatoplasty, plasma eardrum perforation was performed with the diameter of the aperture 2-3 mm, which was decided by observing viscosity of tympanum fluid under microscope or otoscope. RESULT: Compared with the simple cleft palate repairing palatoplasty, the perforation in the observation group could keep for 6-8 weeks after the surgery. Followed up 6 months later, the children in observation group improved better in terms of hearing restoration and tympanometry. CONCLUSION By means of plasma eardrum perforation homochronous with palatoplasty, the children of cleft palate with secretory otitis media could improve hearing and tympanometry in a short period, recovering the function of middle ear.
Acoustic Impedance Tests ; Child ; Cleft Palate ; surgery ; Ear, Middle ; Humans ; Middle Ear Ventilation ; Otitis Media with Effusion ; surgery ; Palate ; surgery ; Tympanic Membrane Perforation

Objective To evaluate prenatal sonograpic findings and clinical value in fetus with congenital portal-hepatic venous shunt.Methods The prenatal ultrasonographic characteristic of ten cases of congenital portal-hepatic venous shunt were analyzed and summarized.Results Three cases had portal vein-left hepatic venous shunt,two cases with portal vein-right hepatic venous shunt,two cases with situs inversus of left right portal vein,and three cases with multiple shunt among portal and hepatic veins.All the 10 cases with portal-hepatic venous shunt had common sonographic features,①Two-dimensional ultrasound revealed the distal hepatic veins.The hepatic vein and portal vein were both visualized on the same plane and connected by an anechoic area.②Vascular signal were noted inside the anechoic area.③Blood flow inside the anechoic area demonstrated by spectral Doppler was similar to that of the portal vein.Conclusions Prental ultrasonography is valuable in the diagnosis of fetal portal-hepatic venous shunt.

AIM:To study the effects of thrombopoietin (TPO) and other hematopoietic cytokines on platelet activation. METHODS: Using fluorescent-labeled monoclonal antibodies and flow cytometry.RESULTS: The per- centage range of platelets activated by 120 ng/mL TPO was 8. 89% -39. 92%,mean value was 17.43%; However, there were no effects of TPO 40 ng/mL and GM - CSF 100 ng/mL and IL- 3 100 ng/mL on platelet activation. CONCLUSION: Higher concentration of TPO directly stimulated platelet activation.