Objective To study the value of 99Tcm-DTPA dynamic renography in evaluating the function of duplex kidneys in pediatric patients.Methods Twenty-five pediatric patients with duplex kidneys diagnosed by ultrasound or MR urography (MRU) were included (9 males,16 females; mean age:(23.80 ± 20.97) months,range:2-72 months).Twenty patients (9 males,11 females; mean age:(32.95 ± 23.58) months,range:2-72 months) with urinary tract infection but without duplex kidneys confirmed by ultrasound or MRU were chosen as control group during the same period of this study.All patients and controls were divided into two subgroups according to their ages (group Ⅰ,0-24 months; group Ⅱ,25-72 months).The research was approved by the ethics committee,and all patients' parents (or guardians) signed informed consents.The time-activity curve was generated on the dynamic imaging data automatically with GFR calculated.The uptake rates of the upper and lower moieties were measured by drawing the corresponding ROIs in the duplex kidney.Dunnett-t test was used for statistical analysis.Results There were 25 patients with 26 duplex kidneys (1 case bilateral),16 on the left and 10 on the right.The time-activity curve of 6 cases was normal,9 with continuously upward type,4 with high level plateau type,2 with parabolic type and 5 with low level plateau type.There were 19 abnormal kidneys in group Ⅰ and 7 in group Ⅱ,and 20 kidneys in each control subgroup.The GFR of patients with normal renography was (78.81 ± 15.97) ml/min (group Ⅰ) and (64.68 ± 11.15) ml/min (group Ⅱ),continuously upward type was (72.11 ±22.76) ml/min (group Ⅰ) and (63.41 ± 16.42) ml/min (group Ⅱ),high level plateau and parabolic types were (68.74 ± 16.17) ml/min and (65.26 ± 15.27) ml/min in group Ⅰ,respectively.There was no statistically significant difference between the GFR of different renography type groups and that of the controls (group Ⅰ:(79.35 ±13.31) ml/min,group Ⅱ (76.46 ±9.69) ml/min;all P ＞0.05).The GFR of patients with low level plateau type was (45.83 ± 10.17) ml/min (group Ⅰ) and (45.53 ± 10.42) ml/min (group Ⅱ).There was statistically significant difference between the GFR of two subgroups of low level plateau type and that of control group,respectively (both P ＜ 0.05).Among the 26 abnormal kidneys,23 could be separated into upper and lower moieties.Among the 23 duplex kidneys,15 cases had uptake rate less than 10％ of that of the whole kidney,5 cases ranging from 10％ to 30％,and 3 cases greater than 30％.Conclusions Quantitative evaluation of duplex kidney functions can be performed with 99Tcm-DTPA renography.It may provide important information for the management of pediatric patients with duplex kidneys.

Objective:Colorectal cancer is one of the common gastrointestinal tumors.Recent studies have shown that, the expression of PDEF can promote the differentiation of Secretory progenitor cells to goblet cells in the intestinal tissue.Therefore the oc-currence of colorectal cancer may be related to expression of PDEF.In this study,we tried to investigate the effects of proliferation and invasion after interference targeting prostate-derived ETS factor in colorectal cell lines HT29.Methods: HT29 cells were transiently transfected with PDEF shRNA plasmids and blank control plasmid via cathodolyte liposome transfection method.By fluorescence microscopy,RT-PCR,Western blot technique to detect the expression of PDEF mRNA and protein in normal control group,blank control group,shRNA group.The proliferation and invasion ability of HT29 cells after transfection were assessed by MTT assay and Transwell invasion assay respectively.Results: Green fluorescent protein was observed in blank control plasmid group and shRNA plasmid group.Western blot showed the reduced PDEF protein expression compared with normal control group and blank control group.Interference PDEF gene expression can significantly promote the proliferation of HT29 cells (P<0.05).The ability of cell invasion in interference group was significantly higher than the normal control group and blank control group after 48h ( P<0.05).Conclusion:Interference PDEF in HT29 cells can promote cell proliferation and invasion.

Skeletal muscle is the largest tissue in the human body, and it plays a major role in exerting force and maintaining metabolism homeostasis. The role of muscle transcription factors in the regulation of metabolism is not fully understood. MondoA is a glucose-sensing transcription factor that is highly expressed in skeletal muscle. Previous studies suggest that MondoA can influence systemic metabolism homeostasis. However, the function of MondoA in the skeletal muscle remains unclear.

Skeletal muscle is the largest tissue in the human body, and it plays a major role in exerting force and maintaining metabolism homeostasis. The role of muscle transcription factors in the regulation of metabolism is not fully understood. MondoA is a glucose-sensing transcription factor that is highly expressed in skeletal muscle. Previous studies suggest that MondoA can influence systemic metabolism homeostasis. However, the function of MondoA in the skeletal muscle remains unclear.

Objective:To construct an early rehabilitation program suitable for patients with cervical spondylosis after minimally invasive cervical laminoplasty and explore its clinical effect, in order to promote postoperative spinal cord function recovery and reduce complications.Methods:The early rehabilitation program after minimally invasive cervical laminoplasty was constructed by literature search, research group discussion and two rounds of Delphi expert correspondence. The convenient sampling was used to select 136 patients who underwent minimally invasive cervical laminoplasty by the posterior cervical approach who were admitted to the First Affiliated Hospital of Zhengzhou University from May 2018 to May 2020. They were divided into control group and observation group by the random number table method, with 68 cases in each group. The control group underwent rehabilitation according to the conventional rehabilitation methods after cervical spine surgery, while the observation group was given the early rehabilitation program intervention after minimally invasive cervical laminoplasty. They were followed up 12 months after surgery. The differences in Japanese Orthopaedic Association Scores (JOA) score, Neck Disability Index (NDI) score and Visual Analogue Scale (VAS) for axial pain were compared between the two groups before intervention and 12 months after surgery.Results:There were no statistically significant differences in the JOA, NDI and VAS scores between the two groups before intervention ( P>0.05) . After 12 months of follow-up, there was no statistically significant difference in the cervical spine JOA score between the two groups ( t=1.655, P=0.101) . The NDI score of the observation group [ (17.68±5.73) ] was lower than that of the control group [ (27.39±11.01) ] , and the difference was statistically significant ( t=-5.846, P<0.01) . The VAS score of the observation group [ (1.59±0.86) ] was lower than that of the control group [ (2.31±1.12) ] , and the difference was statistically significant ( t=-3.801, P<0.01) . Conclusions:The early rehabilitation program after minimally invasive cervical laminoplasty constructed in this study is scientific and reliable, which is helpful to improve the cervical spine function and daily activities of patients with cervical spondylosis after surgery and reduce the occurrence of axial pain.

Objective: To prepare peptide minotope-based recombinant diagnostic antigen of Epstein-Barr virus (EBV) infection and evaluate its antigenicity preliminarily. Methods: With Trx at the N-terminal and His tag at the C-terminal, the peptide minotope of EBV (GP125, F1, A2, A3C2) was expressed in Escherichia coli and purified by affinity and anion exchange chromatography (designated 'H58’); based on antigenicity of H58 identified by Western blotting (WB), we constructed and evaluated a novel early diagnostic ELISA for EBV infection. Results: The soluble H58 protein with high concentration (2.8 mg/ml) and purity (99.01) was obtained; WB analysis found that there was an obvious band (28 ×10³) on the NC membrane, using H58 anti-Trx monoclonal antibody or acute-phase sera of EBV infection as the first antibody. With the novel ELISA, 50 positive sera of EBV infection and 50 negative sera were detected, displaying that the grouping of OD value of positive serum (95%CI: 1.233-1.489) and negative serum (95%CI: 0.113-0.159) was different (P<0.05) with the sensitivity 98.0%, specificity 96.0% and kappa value 0.940. Conclusions By E. coli expression and affinity and ion exchange chromatography purification, the peptide minotope-based recombinant diagnostic antigen of EBV infection was obtained with excellent antigenicity, which could be applied for serological detection of EBV infection.