AIM:To establish a capillary electrophoresis with field-amplified sample stacking method for the separation and determination of aconitine alkaloid in Xiaohuoluo Pills. METHODS: An uncoated fused-silica capi-llary column(50 ?m i.d.?43 cm,effective length 35 cm) was used.The running buffer was 60 mmol/L phosphate electrolyte solution(pH=6.0)-acetonitrile(8(∶)2).The ruuning voltage was 10 kV and the capillary inlet was dipped in water for 5 s with 3.5 kPa prior to electrokinetic injection(12 kV,30 s).The detection wavelength was 235 nm. RESULTS: This method allowed 400 fold enrichment of aconitine alkaloid.A good linear relation was obtained in the range of 37.5～2.4?10~3 ng/mL (r=0.999 5),with the detection limit of 9.4 ng/mL.The average recovery was 97.6% with the RSD of 1.9%. CONCLUSION: The method is simple,rapid,specific and selective with high stacking efficiency;it provides a new reliable means for production and quality control of Xiaohuoluo Pills.

Objective To compare the clinical and MRI characteristics of pituitary macroadenoma between male and female patients. Methods Two hundred and thirty patients with pathologically diagnosed pituitary macroadenomas (104 mlaes and 126 females) were divided into two groups according to different gender. Patient age, the ratio of intratumoral hemorrhage and cavernous sinus invasion, and tumor volume were calculated in each group and compared between the two groups. The constitution of different pathological types was also compared. Results The age, ratio of intratumoral hemorrhage and cavernous sinus invasion, and tumor volume was (44.8±13.2) years old, 21.15% (22/104), 50.00% (52/104), and 6100.48 (mm3, median) in the male group and (44.8±11.9) years old, 28.57% (36/126), 48.41% (61/126) and 5037.05 (mm3, median) in the female group, respectiveluy. There was no statistical difference between the two groups regarding to patient age, the ratio of intratumoral hemorrhage and cavernous sinus invasion, tumor volume, and constitution of pathological types (P=0.972, P=0.197, P=0.811, P=0.189, P=0.093, respectively). Conclusion Pituitary macroadenomas show no gender preference in clinical and MRI characteristics.

Objective:To observe the effect of anti-lymphocyte globulin(ALG)combined with cyclosporin A(CsA)in treatment of severe aplastic anemia(SAA).Methods:Forty-three patients with SAA received ALG [15mg/(kg?d)once a day for5d],CsA [5mg/(kg?d)for6months]and androgen(80mg,tid)for more than6months.Results:After6months,the blood corpuscle counts of patients increased(P

Aim To observe the effect of 5-FU and L-Arg on the human liver carcinoma model in nude mice and study the mechanism.Methods We established the human liver carcinoma model in nude mice with BEL-7402 cell and gave normal saline,5-FU,5-FU+L-Arg by intraperitoneal injection. The tumor size was measured.The necrotic degree and range were observed by the microscope. Immunohistochemistry method and chemical colorimetry were performed to determine the expression and activity of iNOS. Nitrate reductase method was adopted to test the concentration of NO in the tumor tissue. The one-way ANOVA,Bonferroni and Kruskal-Wallis H tests were adopted for the statistical analysis.Results 5-FU combined with L-Arg promoted the tumor suppressive action apparently. The necrotic range enlarged. The expression and activity of iNOS increased. The production of NO also increased.Conclusions 5-FU combined with L-Arg has agonist effects on the inhibition of the growth of human liver carcinoma in nude mice.It may be related with inducing the synthesis of iNOS and the increase of NO.

Objective To explore the effect of health education improvement on promotion of timely vaccination among migrant children in community and to provide references for community health service center.Methods A total of 155 migrant children's parents were recruited from July 2015 to February 2016,and improved health education intervention was carried out on the basis of routine health education to promote parents' health belief on timely vaccination.The compliance of parents' participation in health education activities,the health belief of parents themselves on timely vaccination at different stages of intervention and parents' satisfaction,and the rate of timely vaccination of migrant children before and after intervention were evaluated.Results Regarding the compliance of health education activities for parents in 12 weeks,the first 3 weeks was 85％ ～ 91％,the last 4 weeks were 95％ and above.Six months after intervention,the score of parents' health belief on timely vaccination was higher than that after 3 months of intervention and before intervention,and the difference was statistically significant (P＜0.05).The timely vaccination rates in the study group for migrant children was higher than that in the historical groups (P＜0.05).After intervention,the satisfaction rate for parents was 95.484％.Conclusion Health education improvement could effectively promote parents' health belief on timely vaccination,satisfaction rate and rate of timely vaccination among migrant children.

Training pathologist's ability of rapid intraoperative diagnosis of central nervous system tumors is of great importance to decide ranges of clinical operation and to promote pathological diagnosis level.Pathologist made frozen section,fully understood the clinical data and operation,mastered correct method of slice reading,learned comprehensive analysis,and exercised psychological quality thus to make rapid intraoperative diagnosis correctly.

Objective: To investigate the expression of histone deacetyase4 (HDAC4) in human liver carcinoma cell line Bel-7402 and to explore the regulatory effects of HDAC4 on the proliferation and differentiation of Bel-7402. Methods: Carcinoma cells Bel-7402 was treated with different concentrations of sodium phenylbutyrate (SPB), an inhibitor of HDAC4. Expression of HDAC4 mRNA in Bel-7402 cells was analyzed by RT-PCR before and after SPB treatment. Reverse microscope was used to observe the morphological changes of Bel-7402 cells. MTT assay and flow cytometry were adopted to describe the proliferation and cell cycle of Bel-7402 cells. Expression of P27 protein was determined by immunohistochemical method. The statistical analysis was performed using one-way ANOVA and student t test. Results: SPB significantly decreased the expression of HDAC4 in Bel-7402(0.88?0.13) vs (0.12?0.04), P

Purpose To study the clinicopathological features of extraventricular neurocytoma. Methods 12 cases of extraventricular neurocytoma were retrospectively analyzed for their clinical information and histopathological features. Results The distribution of ex-traventricular neurocytoma was as follows: cerebral hemisphere (5 cases), right thalamus (2 cases), cerebellar vermis (2 cases), saddle area (2 cases), and spinal cord (1 case). The histological features were round uniform cells, round or oval nuclei, with peri-nuclear clearing commonly seen. Neuropil-like structure, ganglion cells or ganglion-like cells, perivascular rosettes, calcification, cap-illary wall thickening and hyaline degeneration and other pathological changes were also observed. All cases showed strong immunoreac-tivity for Syn. 7 cases exhibited Ki-67 labeling index of≤2%, and other 5 cases showed 3% ~8%. 3 cases were performed with fluo-rescence in situ hybridization ( FISH) to detect loss of chromosome 1p/19q, but no loss was detected. Postoperative follow-up for 4 months to 4 years showed one case had tumor recurrence and intracranial spread in postoperative 2 years. Conclusions Extraventricu-lar neurocytoma shares similar histological features with central neurocytoma, but often more complex. Ganglion cells or ganglion-like cells, calcification, capillary wall thickening and hyaline degeneration are more frequently seen, but neuropil-like structure is less seen. Extraventricular neurocytoma needs differential diagnoses from oligodendroglioma, ependymoma, dysembryoplastic neuroepitheli-al tumor and others. Immunohistochemistry and chromosome 1p/19q loss can help in the differential diagnosis. The treatment for ex-traventricular neurocytoma is grossly total resection, with post-operative radiotherapy for subtotal resection, atypical or recurrent cases.

Objective To investigate the clinical therapeutic effect of Shenqi Fuzheng injection for treatment of patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD).Methods A prospective clinical study was conducted. Fifty-eight consecutive patients with AECOPD were admitted in Departments of Respiratory Disease and Critical Care Medicine in Zhuozhou City Hospital of Hebei Province from January 2012 to December 2013. They were randomly divided into western medicine (WM) control group (28 cases, the routine treatment of WM) and integrated traditional Chinese medicine (TCM) with WM group (30 cases, on the basis of conventional therapy, Shenqi Fuzheng injection 250 mL intravenous drip was given once a day for a therapeutic course of 10 days). The duration of mechanical ventilation, the successful rate of weaning from ventilator, the rate of using ventilator again after weaning, the length of stay in intensive care unit (ICU), and mortality were recorded respectively in the two groups. Before and after treatment, the arterial blood gas analysis, acute physiology and chronic health evaluation Ⅱ (APACHE Ⅱ) score, clinical pulmonary infection score (CPIS), pulmonary function and dyspnea score were evaluated. Results Compared with the WM control group, the duration of mechanical ventilation (hours: 104±16 vs. 125±24) and the length of stay in ICU (days: 6.3±2.1 vs. 7.2±3.6) were significantly shorter, the rate of successful weaning from ventilator was obviously higher [73.3% (22/30) vs. 60.7% (17/28)], and the rate of using ventilator again after weaning was remarkably lower [13.3% (4/30) vs. 28.6% (8/28)] in the combined TCM and WM group, the differences between the two groups being statistically significant (allP < 0.05); the mortality was lower in the combined group [10.0% (3/30) vs. 10.7% (3/28)], but there was no statistically significant difference (P > 0.05). Compared with those before treatment, the pH value, arterial partial pressure of oxygen (PaO2), forced expiratory volume in 1 second (FEV1), forced vital capacity(FVC) and the ratio of FEV1/FVC were all significantly higher in the two groups after treatment, while the partial pressure of arterial carbon dioxide (PaCO2), APACHE Ⅱ score, CPIS score, residual volume/total lung capacity (RV/TLC), and the dyspnea score were all lower in the two groups after treatment, the more obvious changes in levels being after 10 days of treatment in combined TCM and WM group [pH: 7.44±0.04 vs. 7.40±0.08, PaCO2 (mmHg, 1 mmHg = 0.133 kPa): 59.1±11.9 vs. 68.1±12.4, PaO2 (mmHg): 70.5±6.9 vs. 65.1±7.4, APACHE Ⅱ score: 14.5±4.2 vs. 17.4±2.2, CPIS score: 5.3±2.4 vs. 7.6±1.4, FEV1 (L): 1.60±0.47 vs. 1.54±0.34, FEV1/FVC: (65.33±2.65)% vs. (62.00±3.25)%, FVC (L): 1.72±0.21 vs. 1.66±0.21, RV/TLC: (42.13±1.67)% vs. (43.12±0.95)%, dyspnea scores: 1.71±0.54 vs. 2.32±0.65, allP < 0.05].Conclusion Shenqi Fuzheng injection possesses certain clinical value in treatment of patients with AECOPD, as it can obviously improve the pulmonary function and the data of arterial blood gas analyses, and effectively relieve the clinical symptoms.

Objective To detect dengue virus infection by serological method and to determine the sequences of E gene of dengue virus isolated from Guangzhou in 2006.so as to clarify the possible origin of dengue fever.Methods IgM and IgG antibodies to dengue virus were detected by immunochromatographic test(ICT);NSI antigen and IgM antibody were detected by enzyme-linked immunosorbent assay(ELISA).The virus was cultured and isolated from the serum samples within 2 days using C6/36 cell lines and was identified by immuno-fluorescence assay(IFA)and RT-PCR.The E gene of isolated virus DV1-GZ42/06 was sequenced;homological analysis and phylogenetic tree analysis were performed by comparing with the reference strains and epidemic virus strains.Results The positive rates of IgM and IgG of dengue virus in patients were 89.5%(433/484)and 38.0%(184/484)by ICT,respectively.The positive rates of NS1 antigen were 92.7%(38/41)in day 1 to day 2,83.3%(70/84)in day 3 to day 5,and 10.9%(5/46)in day 6 to day 10;and the IgM detection rates were 2.4%(1/41),51.2%(43/84)and 97.8%(45/46)at the same period by ELISA.Twenty-five strains of dengue virus were isolated from 41 serum samples(6 1.O%)and were identified as type 1 dengue virus by IFA and RT-PCR.The sequencing and phylogenetic analysis of the E gene showed that the homology between the isolated Guangzhou/42/06 strain and standard strain Hawaii/45 was 94.6%.and it had a high homology with the Thailand/NI09V104,Vietnam/06.and Vietnam/07 isolates(99.0%,98.6%and 98.6%,respectively)and belonged to the same cladogram,but had low homology with the isolated strain from Guangdong before 2006.Conclusions The detection of NS1 antigen is important in the early diagnosis of dengue fever.The outbreak of dengue fever in Guangzhou in 2006 was possibly caused by the cases from neighboring countries.