Myelodysplastic syndromes(MDS) are heterogeneous clonal stem cell disorders.There are about 40 ％-60 ％ MDS patients with abnormal karyotype.To analyze the clone category is not only useful for diagnosis and evaluating prognosis,but also helpful for the selection of optimal therapy strategies to improve the treatment efficacy.

Objective: To explore the value of histogram features based on plain and enhanced CT for differential diagnosis of benign and malignant thyroid nodules. Methods: A total of 132 patients with 140 thyroid nodules confirmed by postoperative pathology were retrospectively analyzed. The level with the largest axial focus was selected, and then Mazda software was used to sketch ROI along the edge of focus and perform histogram analysis to obtain 9 parameters. The parameters were compared between benign and malignant nodules, and then statistically significant gray-scale histogram parameters were used to analyze their value of differentiating benign and malignant thyroid nodules. Results: On plain CT, the mean value, the 10th, 50th and 90th percentiles of malignant nodules were higher than those of benign ones (all P<0.05), while on enhanced CT, the mean value, variance, and the first, 10th, 50th and 90th percentiles of malignant nodules were all higher than those of benign ones (all P<0.05). The average value of malignant nodules was higher than that of benign ones by the first, 10th, 50th and 90th percentage points, and the variance of benign group was higher than that of malignant group (all P>0.05). Among the parameters of CT plain and enhanced histogram, the 10th percentile AUC was the highest of 0.68, and the sensitivity of identifying benign and malignant thyroid nodules was 74.32%,and specificity was 62.12%. Conclusion: CT histogram analysis can be used as an important auxiliary means to identify benign and malignant thyroid nodules.

Objective To discuss clinical characteriistics of APE and therapeutic effect of thrombolysis or anticoagulation so as to reduce false or misdiagnosis rate and improve survival rate.Methods The clinical data of 122 inpatients with APE were reviewed.Results The most common risk factors were deep venous thrombosis,age over 60 years old,cardiac disease,surgical operation,diabetes,fracture,long-term immobilization,etc.The most frequent symptom was dyspnea.D-dimmer measure had high sensitivity.By means of color Doppler echocardiography,spiral computed tomography,pulmonary ventilation/perfusion scanning,the cases were finally diagnosed.Positive treatment of thrombolysis and anticoagulation could reduce mortality.Conclusion As clinical manifestations of APE are non-specific,clinician should be alert.D-dimmer measue could be screening.The standardized treatment can improve prognosis significantly.

OBJECTIVETo explore the effect of glimepiride on the glucose uptake as well as glucose transporter (GLUT)-1 and GLUT-3 expression levels of rat mandibular osteoblasts in hyperglycemia.

METHODSPrimary osteoblasts were isolated and cultured. Then, the cells were placed in an osteogenic medium containing two glucose concentrations (5.5 and 16.5 mmol X L(-1)), with or without glimepiride (10 micromol x L(-1)). Glucose uptake was determined by employing 18F-deoxyglucose (18F-FDG) in the cells, and GLUT-1 and GLUT-3 expression levels were evaluated by Western blot analysis.

RESULTSGlucose at 16.5 mmol x L(-1) significantly inhibited 18F-FDG uptake and downregulated GLUT-3 protein expression in osteoblasts. Hyperglycemia increased GLUT-1 protein expression. Glimepiride significantly increased glucose uptake and upregulated GLUT-1 and GLUT-3.

CONCLUSIONGlimepiride enhance the glucose transporter in rat osteoblasts at two different glucose concentrations.

Animals ; Fluorodeoxyglucose F18 ; Glucose ; Glucose Transporter Type 1 ; Hyperglycemia ; Mandible ; Osteoblasts ; Rats ; Sulfonylurea Compounds

Objective To evaluate the impact of different factors on long-term results in cervical carcinoma patients initially treated by surgery followed by radiotherapy. Methods In 1998, 525 cervical carcinoma patients were admitted, among whom 346 patients were first treated by surgery. 302 of these 525 patients were given postoperative radiotherapy. The stage distribution of these 302 patients were: stage I 142;stage Ⅱa 121;stage Ⅱb 23;and stage Ⅲa 16. ~ 60 Co ? or 6MV X-ray was used for radiotherapy. Perpendicular portals were alternately irradiated to 44-50Gy/4-5 week. Extending the portal, increasing the dose after constricting portal, adding intracavitary afterloading irradiation and adjuvant chemotherapy were carried out according to the different clinical requirements. Results The 5-year survival rate was 89.4%,77.7%,56.5% and 56.3% in stage I, Ⅱa, Ⅱb and Ⅲa lesion, respectively(?~2=22.22,P

OBJECTIVETo study the caffeoylquinic acid derivatives from the leaves of Lonicera japonica.

METHODThe compounds were isolated by column chromatography and identified on the basis of physico-chemical constants and spectral analysis.

RESULTFive caffeoylquinic acid derivatives were isolated,and their structures were identified as 3,4-di-O-caffeoyl quinic acid methyl ester (1), 5-O-caffeoyl quinic acid methyl ester (2), 3,4-di-O-caffeoyl quinic acid (3), 1,3-di-O-caffeoyl quinic acid (4) and chlorogenic acid (5), respectively.

CONCLUSIONCompounds 2, 4 were obtained from this genus for the first time. Compounds 1, 3 were obtained from this plant for the first time.

Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Lonicera ; chemistry ; Plant Leaves ; chemistry ; Quinic Acid ; analogs & derivatives ; chemistry ; isolation & purification

Objective:To investigate the application value of one-stitch prophylactic ileostomy in late ileostomy closure.Methods:The prospective randomized control study was conducted. The clinicopathological data of 141 patients with rectal cancer who underwent low anterior resection combined with prophylactic ileostomy in the Affiliated Tumor Hospital of Zhengzhou University from January 2016 to July 2020 were selected. There were 127 patients being selected after excluding 14 cases who did not undergo ileostomy closure. Patients undergoing one-stitch prophylactic ileostomy were divided into observation group, and patients undergoing traditional ileostomy were divided into control group. Observation indicators: (1) grouping situations of the enrolled patients; (2) surgical situations of ileostomy closure; (3) postoperative situations; (4) follow-up. Follow-up was conducted using outpatient examination and telephone interview once a month after low anterior resection combined with prophylactic ileostomy to detect complication and death of patients. The end point was at 3 months after ileostomy closure. The follow-up was up to July 2020. Measurement data with normal distribution were represented as Mean±SD, and comparison between groups was conducted using the independent sample t test. Measurement data with skewed distribution were represented as M( P25, P75) or M (range), and comparison between groups was conducted using the Mann-Whitney U test. Count data were described as absolute numbers, and comparison between groups was conducted using the chi-square test. Comparison of ordinal data between groups was conducted using the Mann-Whitney U test. Results:(1) Grouping situations of the enrolled patients: a total of 127 patients were selected for eligibility, aged from 31 to 83 years, with a median age of 64 years. Of 127 patients, there were 66 cases in observation group and 61 cases in control group. (2) Surgical situations of ileostomy closure: all patients from the two groups underwent ileostomy closure successfully. The incision length, operation time, volume of intraoperative blood loss, cases with abdominal adhesion degree as slight adhesion or obvious adhesion were 4.25 cm(4.00 cm, 5.00 cm), 48.00 minutes(33.75 minutes, 58.00 minutes), 30 mL(20 mL, 50 mL), 34, 32 of the observation group, versus 7.50 cm(7.00 cm, 8.50 cm), 70.00 minutes(57.00 minutes, 80.00 minutes), 30 mL(30 mL, 50 mL), 13, 48 of the control group, showing significant differences between the two groups ( Z=-9.549, -6.133, -2.758, χ2=12.405, P<0.05). (3) Postoperative situations: cases with incision infection of the observation group and the control group were 5 and 13, respectively, showing a significant difference between the two groups ( χ2=4.917, P<0.05). (4) Follow-up: all the 127 patients were followed up for 6-21 months, with a median follow-up time of 10 months. During the follow-up, 3 cases of the control group had postoperative incisional hernia and were cured after conservative treatment. None of patient had anastomotic leakage related complications or died during the follow-up. Conclusion:The one-stitch preventive ileostomy has the advantage of ileostomy closure, which can reduce the operation time, volume of intraoperative blood loss and shorten the incision length effectively, so as to reduce the incidence of postoperative incision infection related complications.

OBJECTIVE: To investigate the ultrastructural changes of the mucosa of eustachian tube in mice and to reveals the influence of eustachian tube on middle ear function and its relavence with otitis media. METHOD: 12 wild type and 12 mutant mice were divided into two groups by age to observe the the ultrastructural changes of the mucosa of eustachian tube. RESULT: Wild type mice exhibited a thick lawn of morphologically normal, distributed cilia in the mucosa of the middle ear at both time points. The cilia of mucosa of middle ear in mutant mice were short, impaired and disrupted. The impairment of the cilia progressed to a much great severity at 6 months compared to 3 months. CONCLUSION Otitis media occurs not only the ciliated cells decreased and the goblet cells increased. More importantly, the ciliary structure was damaged, leading to the dysfunction of the mucociliary transport system and causing otitis media.
Animals ; Cilia ; pathology ; ultrastructure ; Disease Models, Animal ; Ear, Middle ; pathology ; Eustachian Tube ; pathology ; ultrastructure ; Goblet Cells ; Mice ; Microscopy, Electron, Scanning ; Mucociliary Clearance ; Mucous Membrane ; pathology ; ultrastructure ; Otitis Media ; pathology

Objective To assess the correlation between fractional anisotropy(FA) value and α-SMA of renal tissue in patients with chronic kidney disease (CKD).Methods 33 CKD patients underwent MRI standard scanning and diffusion tensor imaging(DTI).FA values were measured on both medulla and cortex of bilateral kidneys on the FA map.24 patients were performed renal biopsy to detect the expression of α-SMA.The integrated optical density of positive-staining region was calculated for each view.The relationship between the FA values and the integrated optical densities of α-SMA was analyzed by using Bivariate correlation analysis.Results There was a significant negative correlation between the cortical FA values and the integrated optical densities of α-SMA (P=0.001).There was a significant negative correlation between the medullary FA values and the integrated optical densities of α-SMA (P=0.035).Conclusion FA value of DTI was correlated with α-SMA of renal tissue in patients with CKD.

Objective To evaluate the result of early repair for urinary fistula caused by iatrogenic injury to ureter,bladder and urethra during resection of rectal cancer.Methods We retrospectively analyzed 26 cases of urinary fistula after resection of rectal cancer patients encountered in Department of General Surgery,Henan Tumor Hospital from October 2005 to May 2016.Urinary fistula was divided into four types according to the site of fistula.Results Surgery was performed ever after the diagnosis of the fistula was identified.In type Ⅰ fistula (6 cases of posterior urethral fistula) the treatment was stent placement and packing of the greater omentum.2 cases of bladder top fistula (type Ⅱ),were treated by fistula repair and cystostomy.Type Ⅲ involved 12 cases (bladder triangle fistula).The treatment was ureter stenting replantation,and cystostomy Type Ⅳ:ureteral fistula in 6 cases,the treatment was end-to-end anastomosis and stenting.After surgery 24 cases were cured and 2 cases (all of type Ⅲ fistula) ended up with permanent bilateral ureterocutaneostomy.Conclusion It is safe and effective to make early remedy repair for ureter,bladder and urethral fistula iatrogenically during rectal cancer resection.