Objective:To differentitate the myogenic from arthrogenic component of knee contracture resulting from being immobilized in extension for 4 weeks. Method:Ten rabbits were used as experimental animals. The right hindlimbs were immobilized with knee in extension for 4 weeks, while the left severed as controls. The range of motion ( ROM ) of both knees were measured before and after myotomy. Result:In immobilized and control hindlimbs, the mean ROM of the knee were 79.5°and 138.5°, respectively. The gain of ROM after myotomy in immobilized and control hindlimbs were 8.7° ±0. 45°, 11.6° +0.58°, respectively. Only 14.75% of the limitation of motion in ROM were accounted for by the myotomy component. Conclusion:After immobilizing for 4 weeks, the ROM of knee was significantly limited. However, the myogenic part was small and not different from normals.

Objective To differentiate the myogenic and arthrogenic components of knee contracture resulting from being immobilized in extension for 4 weeks.Method Ten rabbits were used as experimental animals. The right hindlimbs were immobilized with the knee in extension for four weeks, while the left served as controls. The range of motion (ROM) of both knees was measured before and after myotomy.Result In immobilized and control hindlimbs, knee mean ROM were 79.5° and 138.5° respectively. The gain of ROM after myotomy in immobilized and control hindlimbs were (8.7± 0.45)° , (11.6± 0.58)° respectively. Only 14.75％ of the limitation of motion in ROM was accounted for by the myotomy component. Conclusions After immobilized for 4 weeks, the ROM of knee was limited.However,the myogenic part was small and not different from normals.Because this study reports on only one time point,further investigations are needed.

ObjectiveTo investigate the effect of early rehabilitation combined with electrotherapeutics on motor function of patients with acute stroke.Methods90 patients with acute stroke were randomly divided into the treatment group and control group with 45 cases in each group. All patients of two groups were treated by routine medication, the cases of the treatment group were added with physical therapy and electrotherapeutics. The motor function of all patients was assessed before and one month after treatment.ResultsAfter treatment, the scores of Fugl-Meyer Assessment, Modified Barthel Index and NFI of all patients in the two groups improved obviously, but the effect of the treatment group was superior to that of the control group ( P<0.05).ConclusionEarly rehabilitation combined with electrotherapeutics can improve the motor function of patients with acute stroke.

Objective To evaluate the feasibilities and advantages of different concentrations of sodium alginate (SA) solutions as a submucosal injection solution for endoscopic submucosal dissection (ESD). Methods In vitro study, different concentrations of sodium alginate solutions and normal saline were injected into submucosal of resected porcine esophagus and stomach respectively, then observe and measure the heights of each injection induced mucosal elevations, and their changes over time. In vivo study, the mimic ESD were conducted in healthy pigs to evaluate the mucosal elevation effect and other assistant effects of sodium alginate as a submucosal injection solution. Results The elevation heights of the experiment groups injected with SA solutions were much higher than the control group injected with normal saline. Specially, the elevation created by 1 % SA in porcine esophagus was significantly higher than that of normal saline (P < 0.01) and the elevation created by 3 % SA was significantly higher than that of normal saline in porcine stomach (P < 0.001). In the mimic ESD experiment, mucosal elevation with clear margin occurred immediately after injection with SA solution. And the durable submucosal fluid cushion created by SA protected deeper tissues while facilitating ESD procedure. Conclusion The elevation heights created by SA solutions were greater and more durable than that created by normal saline, which were crucial for ESD. The viscosity property enabled SA to form a stable protective cushion and prevent bleeding by squeezing tissue around the wound, which may decrease perforation and bleeding rate during ESD procedure. Therefore, sodium alginate can be an ideal clinical submucosal injection solution.

Objective To investigate the effects and molecular mechanisms of decorin(DCN),imatinib mesylate,and sunitinib malate on the malignant phenotype of gastrointestinal stromal tumor cells.Methods Western blotting was used to detect changes in the expres-sion of DCN and its downstream proteins after DCN overexpression and treatment with imatinib mesylate and sunitinib malate alone or in combination in gastrointestinal stromal tumor cells(GIST-882).Cell counting kit-8,scratch,and Transwell assays were performed to validate the changes in cell proliferation,migration,and invasion abilities after DCN overexpression and treatment with imatinib mesylate and sunitinib malate alone or in combination in GIST-882 cells.Results Compared with the control group,DCN overexpression and treatment with imatinib mesylate and sunitinib malate alone or in combination in GIST-882 cells increased the expression levels of DCN protein,decreased the expression levels of epidermal growth factor receptor(EGFR),phosphorylated EGFR(p-EGFR),extracellular signal-regulated kinase 1/2(ERK1/2),and phosphorylated ERK1/2(p-ERK1/2)proteins,and significantly reduced cell proliferation,migration,and invasion abilities.Conclusion DCN overexpression and treatment with imatinib mesylate and sunitinib malate alone or in combination affect the MAPK signaling pathway by downregulating the expression of EGFR,thereby regulating the proliferation,migra-tion,and invasion abilities of gastrointestinal stromal tumor cells.