Objective To compare the differences of temperature results between mercury thermometer and infrared thermometer. Methods The temperature of three parts was recorded respectively by mercury thermometer in axilla and infrared thermometer in forehead and earlobe on the same patient, totally 98 patients were recorded in ICU. Results There was statistical difference of three parts' temperature in 98 cases. It could be assumed that axilla temperature ＞ earlobe temperature ＞ forehead temperature;There was no statistical difference of three parts' temperature in 17 cases with temperature 38.0～38.9 ℃ by mercury thermometer, but the difference had clinical significance. It could be assumed that axilla temperature ＞ earlobe temperature ＞ forehead temperature; There was statistical difference of three parts' temperature in 30 cases with temperature at 37.0～37.9 ℃ by mercury thermometer. It could be assumed that axilla temperature ＞ earlobe temperature ＞ forehead temperature. There was no statistical difference of three parts'temperature in 51 cases with temperature at 35.0～36.9 ℃ by mercury thermometer. Conclusions Temperature of the patients with normal axilla temperature could be monitored by infrared thermometer instead of mercury thermometer,but it is not applicable to the patients with fever.

Objective:To investigate the possible interaction of Lp(a), and Lp(a) circulating immune complexes (CIC) 〔Lp(a)-CIC〕 level with triglyceride (TG). Methods:Plasma Lp(a), Lp(a)-CIC and LDL-CIC levels were determined by ELISAs in 232 patients with various dyslipidaemias, respectively. Results:Hypertriglyceridaemic patients exhibited the lowest plasma Lp(a) levels, while hypercholesterolaemic patients exhibited the highest. Patients with mixed hyperlipidaemia had intermediate serum Lp(a) concentrations, which were significantly lower than those in the controls. Interestingly, we also found that hypertriglyceridaemic patients exhibited the lowest plasma Lp(a)-CIC and LDL-CIC levels, while hypercholesterolaemic patients exhibited the highest. TG levels were negatively correlated with Lp(a) (r=-0.15, P

Objective To investigate the effects of Duyiwei capsule combined with traditional bone setting manipulation treatment of senile femoral intertrochanteric fracture clinical effect, for clinical elderly femoral intertrochanteric fracture clinical treatment in patients with reference. Methods 86 elderly patients with intertrochanteric fracture in Yuyao Hospital of Traditional Chinese Medicine from April 2014 to May 2015 were randomly divided into control group and observation group, 43 cases in each group. The control group using tibial tubercle traction, after traction with the traction arch to maintain. The observation group was treated by TCM manipulation combined with Duyiwei capsule. Continuous treatment for two weeks in two groups. The quality of life and clinical efficacy were compared between the two groups after treatment score, pain score, fracture healing time, hospitalization time, Harris score, evaluation of traditional Chinese medicine treatment of manipulative reduction combined with Duyiwei capsule of senile intertrochanteric fracture clinical effect. Results Two groups of patients after 2 weeks of treatment, The quality of life score, the Harris score of the observation group was significantly higher than the control group, the difference was statistically significant (P<0.05), pain scores were significantly I control group, healing time and hospitalization time were significantly shorter than the control group, the difference was statistically significant (P<0.05). The observation group excellent rate 90.7%was significantly higher than the control group 65.12%, the difference was statistically significant (P<0.05). Conclusion Duyiwei capsule combined with TCM manipulation for treatment of intertrochanteric fracture of femur in the elderly curative effect, effectively improve the patient's clinical experience, is suitable for the elderly femoral intertrochanteric fracture patients with a clinical treatment plan.

Borneol is a traditional Chinese medicine. In the past few years, many studies showed that borneol can improve the bioavailability of other drugs, promoting drugs to cross the blood-brain barrier, so the potential drug interactions between borneol and other medicines have attracted great attention, but the influence of borneol to CYP450 and its isoforms are rarely reported. In this research, male Wistar rats were orally administered by borneol for 7 days, then the mRNA and protein expression and the activities of CYP2D were detected, we also compared the pharmacokinetic parameters of CYP2D's specific substrate between control group and borneol group. The results show that borneol (33, 100 and 300 mg x kg(-1) x d(-1)) have no significant effect on CYP2D, while the activites of CYP2D increased 1.71, 1.97 and 2.89 times comparing to the control group. At the same time, borneol (300 mg x kg(-1) x d(-1)) caused the C(max) decreased 10.6% (P > 0.05), AUC(0-∞) decreased 27.5% (P < 0.01), CL/F increased 41.1% (P < 0.01), V(z)/F increased 23.1% (P > 0.05) of dextromethorphan. Our data provided that borneol speed up dextromethorphan's elimination in vivo. Since the activity of CYP2D can be induced by borneol, the metabolic interactions might happen when borneol and the substrate drug CYP2D are used together.

Objective:To scan the common differentially expressed genes in mice liver tissue following subacute exposure to hepatotoxicants,including alcohol,carbon tetrachloride,BCG & LPS,the alcohol extracts of Rhizoma Dioscoreae Bulbiferae and Senecio scandens Buch.-Ham.,and apply to the rapid toxic evaluation of TCM and other xenobiotics.Methods:The gene expression profiles of mice liver tissue,after respective administration of alcohol,CCl4,BCG associated with LPS,the alcohol extracts of Rhizoma Dioscoreae Bulbiferae and Senecio scandens Buch.-Ham.to healthy mice,were analyzed by use of mouse genome cDNA microarray.The correlations between the common differential expressed genes and the liver injury were analyzed based on the biological functions of those differentially expressed genes.Results:Among all of the five drug administration groups,there were 7 known function genes(BC05200,NM-019410,NM-173019,AB028272,AK088925,AK030862 and AK088816)and 1 unknown function gene(BC069871)differentially expressed.Beside AK088816,the other 7 genes were all down-regulate expressed.Conclusion:The common differentially expressed genes participate in the process of saccharometabolism,apoptosis,cell growth cycle,cytoskeleton and signal transmission,protein folding and protein ubiquitin.The abnormal expression of the common genes were closely correlated to the liver,and might be important for rapid hepatoxicity elucidation of traditional Chinese medicine and other xenobiotics.

Objective To explore the clinical value of transvaginal color Doppler ultrasound(TV-CDS)in the detection of blood flow changes within the ovarian stromal artery in patients with ovarian endometriosis.Methods Blood flow indices within the ovarian stromal artery were measured by TV-CDS in 60 patients and60 normal controls.Results In ovarian endometriosis group,TV-CDS examination showed the color signal pattern was dot-like with high-resistance ovarian stromal arterial flow which manifested significant higher resistance index(RI),pulsatility index(PI)and systolic/diastolic(S/D)ratio than those in normal group(P<0.01).Analysis on clinicopathologic data showed that cystic history and diameter were risk factors affecting the absence of ovarian stromal blood signal,while cystic history,diameter and category were associated with the significant difference of blood flow display area(P<0.05).Conclusions TV-CDS can be used as a non-invasive,convenient and sensitive method for assessing blood flow changes within the ovarian stromal artery,indicating ovarian interstitial damage as well as pathological conditions of ovarian endometriosis that contributes to clinical diagnosis and treatment.

Objective To explore the effect of inferior hypothermy treatment on serum TNF and IL-6 in patients with severe cerebral trauma.Methods 46 patients were randomly divided into two groups:inferior hypothermy group(24 C88e8)and normal group(22 cases).There are the same basic treatments within the two groups,in the inferior hypothermy group we also sive them hypothermy treatment rectal temperature:32～34℃ which need to last for nearly 4～5 days,at the same time we give patients the lyric cocktail.the TNF,IL-6 and GCS grades on the lst and 14th day were tested.Results TNF and IL-6 as compared with normal group are higher than the inferior hypothermy group,the differences between the two groups are of statistical significance(P＜0.01).The difference of GCS grades between the two groups are of stafictical significance(P＜0.05).Conclusion The inferior hypothermy tbempy which inhibits TNF and IL-6 releasing after severe cerebral trauma and the following damages plays a very important role in the cerebral trauma therapy.

Objective To establish a gas chromatographic method for the determination of 41 organophosphorus pesticide retention in ginseng. Methods Samples were extracted with acetonitril, purified by PSA solid phase extraction column, eluted by acetone-normal hexane, detected by FPD, and separated by ZB-1701 capillary column. Temperature for the samples was increased through program and the samples were determined by gas chromatography. Results 41 organophosphorus pesticides showed good linear relation in the range of 5-1000 μg/kg, and correlation coefficient was 0.991-0.998. The average recoveries at the three fortification levels (50, 100, 500μg/kg) were between 69.8% and 99.7% with the RSD between 1.9% and 15.3%. The limits of quantification (LOQ) of the 41 pesticides were between 9μg/kg and 45μg/kg. Conclusion The method developed in this study is with high degree of accuracy, good sensitivity, easy and simple to handle.

Objective To explore the radiosensitivity ofβ-elemene on Colo320 cells transplanted tumor in nude mice and its molecular mechanisms.Methods Colo320 cells transplanted tumor model was established by cell suspension inoculation in nude mice.Then the transplanted mice with 0.8-1.0 cm3 tumor were randomly divided into 4 groups:control,β-elemene (40 mg/kg),radiation (4 Gy),andβ-elemene (40 mg/kg)+ radiation (4 Gy) groups,with four to five mice in each.Tumor weight and morphology were observed in each group.In addition,the apoptosis of tumor cells was measured by TUNEL and the expression of Fas gene was detected by immunohistochemistry.Results The nude mice transplanted tumor model was successfully established.Compared with that in control and simple radiation groups,tumor weight was significantly decreased inβ-elemene combined with irradiation group (P <0.05).At the same time,the apoptosis rate and the expression of Fas gene in tumor cells were significantly increased (P <0 .0 5 )inβ-elemene combined with irradiation group compared with control and simple radiation groups. Conclusion β-elemene could enhance the radiosensitivity of Colo320 cells transplanted tumor in nude mice probably by inducing Fas-mediated apoptosis of tumor cells.

The effects of magnolol (Mag) on hyperglycemia and hyperlipemia, hepatic oxidative stress and cytochrome P4502E1 (CYP2E1) activity of diabetic rats induced by high-fat diet (HFD) and streptozotocin (STZ) were studied. After oral administration of Mag (25, 50 and 100 mg x kg(-1) x d(-1)) for continuous 10 weeks, the blood glucose and lipids (TC, TG and LDL-C) levels, as well as the hepatic CYP2E1 activity and MDA content of diabetic rats, decreased significantly (P < 0.05 or P < 0.01), whereas the oral glucose tolerance and hepatic antioxidant enzymatic activities (CAT and GSH-Px) of diabetic rats, increased significantly (P < 0.05 or P < 0.01). The results indicated that Mag was effective against the hepatic oxidative damage, hyperglycemia and hyperlipemia of diabetic rats induced by HFD and STZ, and the inhibition of Mag on hepatic CYP2E1 activity could be an important mechanism of Mag against hepatic insulin resistance and oxidative damage.