1.Reductive effect of rhPLD2 on PAF content in serum of guinea pig model with chronic asthma
Ling ZHU ; Xuepeng XU ; Chuanxing YU ; Junjin LIN ; Xiaoli HE
Chinese Journal of Immunology 1986;0(04):-
Objective:To study the biologic function of rhPLD2 mutation form.Methods:To adapt the guinea pig chronic asthma model was induced by OVA, the functions of rhPLD2 on PAF was observed through the assay of platelet congregating.Results:rhPLD2 remarkably reduced the lever of PAF in the serum of guinea pig chronic asthma model; compares with the NS group, the P
2.Recombinant Human PLD2(rhPLD2)May Significantly Inhibit Expression of GPI-PLD of Guinea Pigs of Chronic Asthma in vivo
Ling ZHU ; Chuanxing YU ; Weibin ZOU ; Xiaoli HE ; Junjin LIN
Chinese Journal of Biochemistry and Molecular Biology 2007;23(2):116-121
The effect of recombinant human phospholipase D2(rhPLD2)in vivo was investigated on the secretion of serum glycosyl phosphatidylinositol-specific phospholipase D(GPI-PLD)in guinea pigs of chronic asthma．Ater treating the guinea pigs attacked by chronic asthma with rhPLD2,the GPI-PLD activity detection was canrried out by phase separation of human placental alkaline phosphatase in Triton X-114．Compared with the healthy guinea pigs(NS group),the serum GPI-PLD in the guinea pigs of chronic asthma are much higher than that of control groups,P≤0．01．Our results showed that rhPLD2 could significantly reduce the secretion of GPI-PLD when the guinea pigs were attacked by chronic asthma．
Result Analysis
Print
Save
E-mail