Triple negative breast cancer (TNBC)is difficult to benefit from endocrine therapy or tras-tuzumab targeted drug therapy.Biological overexpression of breast cancer susceptibility gene 1,p53 gene,vas-cular endothelial growth factor and microRNA suggests that TNBC is easy to metastasis and recurrence and has a poor prognosis.Exploring the molecular subtypes of TNBC,setting out the treatment plan for subtypes and finding the corresponding monoclonal antibody targets are the research direction of TNBC in the future.

Objective To study the clinical efficacy and the difference of intervention in appendix inflammatory mass by heavenly cool-inducing technique adopted distant and neighboring point selection. Methods Eighty patients of the appendix inflammatory mass with toxic heat syndrome were randomly divided into control group, distant point selection group, neighboring point selection group, and combined group, 20 cases in each group. The control group adopted the conventional western medicine treatment. On the basis of control group, the rest 3 acupuncture groups adopted the heavenly cool-inducing technique for 14 d. The distant point selection group chose Hegu point and Lanwei point;the neighboring point selection group chose Ashi point and Tianshu point (right);the combined group put them together. The longest diameter and three-dimensional volume of inflammatory mass were determined before and after treatment. The serum levels of white blood cells, C-reactive protein were determined at the same time, and then the clinical effective was estimated. Results The longest diameter, three-dimensional volume of inflammatory mass, the serum levels of white blood cells, and C-reactive protein of patients in 4 groups were reduced after treatment. The above indicators in patients of 3 acupuncture groups were lower than the ones in control group, the indicators in combined group were the lowest (P<0.05, P<0.01), and there was no difference between distant and neighboring point selection groups (P>0.05). The invalid number of cases in 3 acupuncture groups were fewer than the one in control group, and the indicators in combined group were fewest (P<0.05, P<0.01), with no difference between distant and neighboring point selection groups (P>0.05). Conclusion There is obvious therapeutic efficacy of heavenly cool-inducing technique to appendix inflammatory mass, while the combination of distant and neighboring point selection is better than the single methods.

To explore the relationship between self-concept and mental health. Methods:398 middle school students were tested by SCL-90 and Song-Hattie Self-concept Scale. Results: The correlation coefficients between the total score of SCL-90 and academic self-concept, non-academic self concept and total self concept were -0.22, -0.21 and -0.23 respectively. Conclusion: Self concept was significantly correlated with mental health in middle school students.

Cervical cancer is a common malignancy in women. Surgery and radiotherapy are the main therapeutic approaches. The recurrence rate of cervical cancer is high and the prognosis is relatively poor. Recurrent cervical cancer can be classified into the central pelvic, lateral pelvic and extra-pelvic recurrence. The management of recurrent cervical cancer mainly depends on previous therapeutic approaches and the recurrence site. Surgical resection is the optimal option for patients who have no history of radiotherapy, if feasible, chemoradiotherapy±brachytherapy can be considered. Patients with central pelvic recurrence after radiotherapy are suitable candidates for pelvic exenteration±intraoperative radiotherapy. However, the indications are highly selective, the incidence of postoperative complications is high and the quality of life is poor. Patients with lateral pelvic recurrence after radiotherapy could be treated by re-radiotherapy or chemotherapy. However, the incidence of serious side effects of re-radiotherapy is higher than that of the initial radiotherapy. The indications for stereotactic body radiotherapy are limited and the surgical skill of the surgeons is demanding. In recent years, radiotherapy technology has become more precise. Image-guided brachytherapy, such as high-dose-rate interstitial brachytherapy and radioactive 125I seed implantation, has achieved high efficacy and safety, which may provide new treatment options for recurrent cervical cancer. In this article, research progress on brachytherapy for recurrent cervical cancer was summarized and reviewed.

Objective To explore the effect of red lip defect repair using orbicularis oris muscles pedicle island flap disc.Methods 100 cases of unilateral pure red lip defect 1/3-2/3,lip lines full of patients were selected as the research subjects.They were randomly divided into two groups,50 cases in each group.Group A treated with debridement surgery to repair.Group B received red lip flap to promote repair treatment.The treatment effect of the two groups, satisfaction, lip survival rate and the degree of obvious scars were compared.Results he operation effect of the two groups was good.The satisfaction rates of group A and group B were 94.00% and 96.00%,respectively.For the lip shape treatment satisfaction, the difference was no statistically significant (P>0.05).The patients of the two groups with lip all alive, and 1 patient in group A with obvious scar, the scar was not obvious in group B.Conclusion For patients with red lip defect, take orbicularis oris muscles pedicle island flap of the oral mucosa or red lip flap to promote repair treatment has relatively significant clinical efficacy, with easy operation, less influence on the patients with facial form, it is worthy of popularizing.

Objective To establish a non-traumatic mouse model of acid aspiration-induced lung injury which al-lows longitudinal studies.Method C57BL/6 mice were anesthetized and orotracheally intubated with a 20 gauge angio-catheter guided by optical fiber.The mice were subsequently placed in the right lateral decubitus position and external com-pression to the left lung was manually applied.A polyethylene catheter was advanced into the right lung and used to instill either hydrochloric acid (2.5μL/g, 0.1 mol/L, pH 1.5) or saline as control.Then the mice were recovered with supple-mental oxygen for 4 hours.The pulmonary physiological function and survival of mice within 2 weeks after surgery were as-sessed.Results Methylene blue instillation showed that the staining fluid went into the right lung of the non-traumatically intubated mice.The survival rate of the mice with non-traumatic instillation was 80%, statistically significantly higher than those with tracheostomy instillation.Histological examination and lung function ( wet/dry ratio, elastance and arterial oxy-gen saturation) assay demonstrated that acid instillation caused a profound pathological changes and functional impairment of the lung.Besides, acid aspiration into the mouse lung caused a significant increase in neutrophil infiltration in mouse pulmonary alveoli and high concentrations of inflammatory factors (TNF-α, IL-6, CXCL1 and CXCL2) in the bronchoalve-olar lavage fluid.Conclusions We successfully established a mouse model of acid aspiration-induced lung injury, which may serve as a reliable model for longitudinally studying pulmonary immune-inflammatory mechanism in humans.

This study was aimed to reveal the relationship of bronchioloalveolar carcinoma (BAC) syndromes and metastasis, in order to provide new scientific basis for syndrome differentiation, treatment and prognostic assessment, which proved the scientificity and feasibility of internal and external inspection. A total of 60 BAC cases were divid-ed into two groups according to the syndrome of deficiency and excess. Another 30 cases of benign pulmonary nod-ules were simultaneous collected in the physical examination as control group. The quality of life (FACT-L) was scored one day before operation for patients in each group. The thoracoscopic technique was used in the biopsy of targeted tissues, in order to reveal indicators related with metastasis. The results showed that the comparison of FACT-L score of patients from two syndromes of BAC and that of the control group indicated that the score of physi-ological state, social/family condition, emotional condition, functional situation, additional situation and total score of the previous one were obviously higher than the latter one (P < 0.01). And the score of patients in the deficiency syndrome group was higher than that of the excess group with statistical significance (P< 0.01). The comparison be-tween the tumor tissues of patients from two syndromes of BAC and the benign pulmonary nodules showed that the VEGF, MMP-2, MMP-9 of the previous one were higher than the latter one (P< 0.01). The comparison between two syndromes showed that all indexes of the deficiency syndrome were higher than the excess syndrome (P < 0.01). It was concluded that syndromes of BAC was related with metastasis.

Anthrax is a fulminating infectious disease .Bacillus anthracis, as the pathogen of anthrax , is a potential ma-terial for biological warfare agents and biological terrors .Immunoprophylaxis and specific theraphies play key roles in cop-ing with anthrax threats.Researches on the mechanism of B.anthracis infection, especially the process of infection , can fa-cilitate the development of novel drugs for anthrax prevention and therapy .Herein, by reviewing research progress , the in-fection process of B.anthracis is introduced and the potential mechanism of anthrax infection is described .Furthermore, the relationship between researches on anthrax infection mechanisms and the development of drugs for anthrax prevention and therapy is also discussed .

Objective To observe and investigate the influence degree of laparoscopic operation for the postoperative body stress and inflammatory state of patients with gastric and duodenal ulcer perforation.Methods 76 patients with gastric and duodenal ulcer perforation were selected as study subjects,and they were divided into control group(conventional open operation group)and observation group(laparoscopic operation group)according to the random number table,38 cases in each group.The serum body stress hormones and antiinflammatory,proinflammatory related indexes of the two groups at first day before operation and at first,third,seventh day after operation were respectively detected and compared.Results The serum IL -2 levels of the observation group at first,third and seventh day after operation were (3.53 ±0.24)μg/mL,(3.25 ±0.22)μg/mL and (4.37 ±0.33)μg/mL,which were higher than those of the control group(F =5.876,P <0.05),while other serum body stress hormones and antiin-flammatory,proinflammatory related indexes were all obviously lower than those of the control group (all P <0.05), there were significant differences between those postoperative evaluation results of the two groups.Conclusion The influence of laparoscopic operation for the postoperative body stress and inflammatory state of patients with gastric and duodenal ulcer perforation is relatively small,and it shows that the bad body stress degree caused by the operation is relatively smaller.

Objective To investigate the epidemiological situation and the mode of inheritance of ankylosing spondylitis (AS).Methods The AS prevalence investigation was carried out from three different areas in Shandong Province to investigate the incidence of the 80 patients' family members of sick group and calculate the heritability of AS.Results After the investigation and calculation we found the incidence of primary relatives was 7.23％,the heritability was 85.94％,the incidence of secondary relatives was 0.94％ and the heritability was 57.4％,the incidence of thirdly relatives was 0.39％,the heritability is 36.2％,the average heritability among primary,secondary and thirdly was 68.47％ ± 1.64％.Separation ratio was 0.088 9.Conclusion AS is a polygenic disease,its average heritability was 68.47％ ± 1.64％ and separation ratio is 0.088 9.By this research,the incidence risk of AS patients' pedigree offspring can be assessed,then strengthen protective factors to reduce the incidence of the disease to provide the basis for eugenics.