1.Effects of AgNO_3 on caducity-resistant from tissue culture of Dedrobium officinale
Jinjin LI ; Junjie LIAO ; Jiyong XU ; Yuling MAI
Chinese Traditional and Herbal Drugs 1994;0(06):-
Objective To solve the caducity question of Dendrobium officinale during the tissue-culture process. Methods Added the different concentration of 0.1 and 2 mg/L AgNO3 into the PLB proliferation medium, buds proliferation medium, and rooting medium separately. The quantity of the ethylene production was to be determined and the situation of the tube seedling growth was to be observed. ResultsAgNO3 can suppress the production of the ethylene, enhance the multiplication speed of PLB and split-up efficiency the clump of bud, and obviously promote the seedling′s growth. It showed that the survival ratio of transplant could enhance as many as two times and display the merit of caducity-resistant. ConclusionAgNO3(1 mg/L) can be taken as the caducity-resistant reagent of D. officinale during the tissue-culture processing.
2.Expert consensus on 3D-printing template assisted CT-guided radioactive 125I seed implantation brachytherapy
Junjie WANG ; Shude CHAI ; Guangjun ZHENG ; Anyan LIAO ; Ping JIANG ; Yuliang JIANG ; Zhe JI
Chinese Journal of Radiological Medicine and Protection 2017;37(3):161-170
As an interstitial brachytherapy,radioactive seed implantation could provide high doses in the local site and minimal doses at surrounding normal tissues.It has become one of the best choice for early stage prostate carcinoma.Radioactive seeds were implanted under the guidance by ultrasound,CT and MRI,featured with surgical and interventional treatment.Based on dosimetry for target and organs at risk,radioactive seed implantation is multiple disciplinary.In order to acquire the accurate and high quality seed implantation,it is necessary to set up a team including surgeons,radiation oncologists,interventional doctors and nuclear medicine doctors.The content of the consensus is as follows:radiation physics and dosimetry,indications,side-effects and 3 D-printing template work-follow.Despite the benefit of radioactive seed implantation for solid carcinoma,there still a compelling need for prospective randomized and stage Ⅲ clinical trials from multiple centers,so as to upgrade the evidencebased level,above all confirm the role of radioactive seed implantation in the comprehensive treatment of tumors.
3.Mechanism of the skin lesions in diabeitic rats
Weidong LIN ; Xiangfang CHEN ; Zhimin LIU ; Shuliang LU ; Yongquan SHI ; Junjie ZOU ; Wanqing LIAO
Chinese Journal of Endocrinology and Metabolism 2010;26(1):62-65
Four weeks after SD diabetic rats were induced by streptozotocin,skin thickness was obviously reduced with obscure multilayer epithelium features.Moreover,the thickness of epidermic layers in diabetic rat skin was significantly thinner than that ofnornlal rat skin at the eighth week[(0.016±0.006 vs 0.041±0.007)mm,P<0.01].The percentage of G2/M phase cells in epidermic layers of diabetic group was significantly lower than that in the normal group.At the twelfth week,skin microangiopathy was easily detected in the diabetic group.The blood levels of advanced glycation end products(AGEs)and malonialdehyde were significantly increased and glutathione decreased in diabetic rats compared with control rats(aU P<0.01),along with the increased contents of local glucose and AGEs in the skin of diabetic rats.These results suggest that the local accumulation of glucose and AGEs seems to be one of the important mechanisms in the pathogenesis of diabetic skin lesions.
4.Preparation and properties of polydopamine coating on the surface of C/C composites
Xiaoling YANG ; Junjie CAO ; Jian YANG ; Xue LIU ; Bo LI ; Xiaoling LIAO
Military Medical Sciences 2016;40(12):941-945,963
Objective Carbon/carbon ( C/C) composites are a potential material for bone repair .To improve the hydrophilicity and biological activity of C/C composites , polydopamine coating was prepared on the surface of C /C composites.Methods By changing the coating time and coating environment ( oxygen or air atmosphere ), a series of polydopamine coating samples were prepared . The water contact angle test , scanning electron microscope , energy dispersive spectrometer , atomic force microscope and protein adsorption test were used to characterize the properties of different samples.Then, the data were analyzed to optimize preparation conditions .Results When the concentration of dopamine was 2.0 g/L,and the coating time was 30 min (oxygen atmosphere) +11.5 h (air atmosphere), the water contact angle was decreased from 64.0°(blank sample) to 25.9°, suggesting that the hydrophilicity was improved greatly . The results of the scanning electron microscope , energy dispersive spectrometer and atomic force microscope showed that the coating was uniform and dense .The amount of protein adsorption per sample was 0.2817 mg.Conclusion The polydopamine coating on the C/C composites improves its hydrophilicity and biological activity , which indicates that the polydopamine coated C/C composites will be a new potential material for bone tissue repair .
5.Prokaryotic expression, purification, identification, and polyclonal antibody prepa-ration of enterohemorrhagic Escherichia coli effector NleB1
Xinxin CHEN ; Xiang LIAO ; Ting SONG ; Wei ZHOU ; Hongmei DAI ; Junjie YUE ; Yu WANG ; Yurui WANG ; Long LIANG
Military Medical Sciences 2014;(10):799-802
Objective To construct a prokaryotic plasmid expressing the recombinant protein of enterohemorrhagic Escherichia coli(EHEC) effector NleB1 and to prepare the polyclonal antibody of mouse anti-NleB1.Methods The nleB1 (990 bp) gene was amplified from the genome EHEC O157∶H7 and cloned into the expression plasmid pET24a to construct the recombinant plasmid pET24a-nleB1 that was transformed into E.coli BL21(DE3).After induction with isopropylthio-gelactoside( IPTG) , the His-tag fusion proteins were purified by Ni+affinity chromatography and gel slices.The polyclonal antibody was prepared by immunizing BALB/c mice with purified recombinant proteins and analyzed by Western blotting and ELISA.Results The pET24a-nleB1 recombinant plasmid was successfully constructed, the fusion protein was ex-pressed and purified,and the polyclonal antibody was obtained by immunizing mice with purified fusion protein.Western blotting and ELISA staining demonstrated that the polyclonal antibody was successfully obtained.Conclusion The prepara-tion of the polyclonal antibody against EHEC O157∶H7 NleB1 will be of help for further studies on the function of NleB1 protein.
6.Standardized Treatment and Shortened Depression Course can Reduce Cognitive Impairment in Adolescents With Depression
Penghui CAO ; Junjie TAN ; Xuezhen LIAO ; Jinwei WANG ; Lihuan CHEN ; Ziyan FANG ; Nannan PAN
Journal of the Korean Academy of Child and Adolescent Psychiatry 2024;35(1):90-97
Objectives:
This study aimed to explore the influence of depression severity, disease course, treatment status, and other factors on cognitive function in adolescents with depressive disorders.
Methods:
Participants who met the inclusion criteria were enrolled in the study. Sociodemographic data of each participant were recorded, including age, sex, and family history of mental disorders. Zung’s Self-Rating Depression Scale was used to assess depression status in adolescents. Moreover, P300 and mismatch negativity (MMN) were used to objectively evaluate the participants’ cognitive function.
Results:
Only 26.8% of the adolescents with depression received standard antidepressant treatment. The latencies of N2 (267.80±23.34 ms), P3 (357.71±32.09 ms), and MMN (212.10±15.61 ms) in the adolescent depression group were longer than those in the healthy control group (p<0.01). Further analysis revealed that the latency of MMN was extended with increased levels of depression in adolescents.The MMN latency was short in participants with depression receiving standardized treatment. Furthermore, the latency of MMN was positively correlated with the severity and duration of depression (correlation coefficients were 0.465 and 0.479, respectively) (p<0.01).
Conclusion
Receiving standardized treatment and shortening the course of depression can reduce cognitive impairment in adolescents with depression.
7.Effects of miR-218-1-3p on Cell Proliferation, Cycle, and Apoptosis of Non-small Cell Lung Cancer
Lan LUAN ; Tiange ZHANG ; Bin HAN ; Wenting LUO ; Fei WU ; Xin LIAO ; Junjie ZHANG ; Yang BAI ; Xinyu CHENG ; Meng TENG ; Cuifang WANG
Journal of China Medical University 2017;46(11):980-983,994
Objective To investigate the effect of miR-218-1-3p on the proliferation,cycle,and apoptosis of A549 cells in non-small-cell lung cancer.Methods miR-218-1-3p was transfected into non-small cell lung cancer A549 cells by LipofectamineTM 2000 Reagent,and the expression of miR-218-3p was detected by real-time PC R.Invasion and migration were assayed using the Transwell method.The effect of miR-218-1-3p on the proliferation of A549 cells was assayed by the MTS method.Changes in the cell cycle and apoptosis of A549 cells transfected with miR-218-1-3p was detected by flow cytometry.Changes in indicators related to cell proliferation,cycle,and apoptosis were detected by fluorescence quantitative PCR.Results Compared to the control group,the cell proliferation of A549 cells was significantly inhibited (P < 0.05) and the proportion of cells in the S and G2-M phases was significantly decreased when miR-218-1-3p was up-regulated.In addition,compared with the control group,the early apoptotic rate was significantly increased by up-regulating miR-218-1-3p.We further detected indicators related to cell proliferation,cycle,and apoptosis and found that CYCLIN-D1 and BCL-2 were significantly downregulated.Conclusion miR-218-1-3p may inhibit proliferation,induce cell cycle arrest,and promote cell apoptosis of non-small cell lung cancer A549 cells by regulating CYCLIN-D 1 and BCL-2.
8.Clinical efficacy of hepatic artery infusion chemotherapy combined with immunotherapy plus target therapy for advanced hepatocellular carcinoma
Hongxiang CAO ; Rui LIAO ; Qiang HE ; Long PAN ; Yi ZHAO ; Yongchen WANG ; Junjie HUANG ; Chenrui WU ; Ruirui SUN ; Ping HUANG
Chinese Journal of Digestive Surgery 2021;20(S2):41-44
Primary liver cancer is one of the common malignant tumors and its mortality ranks third in the world. Because there are no obvious symptoms in the early stage of liver cancer, most patients are diagnosed as advanced stage, without the opportunity of surgical resection. The authors report a case of hepatocellular carcinoma with portal vein tumor thrombus, which reduced significantly after hepatic artery infusion chemotherapy combined with bevacizumab and atezolizumab, showing the safety and efficacy.
9.Patent Analysis of Artemsia Argyi Based on Incopat Patent Database
Lichun YE ; Chenyu WANG ; Qin YUAN ; Junjie HU ; Guohua ZHENG ; Benda XIAO ; Xinan LIAO ; Zhaohua SHI
World Science and Technology-Modernization of Traditional Chinese Medicine 2023;25(6):1881-1894
Artemsia argyi Levl.et Vant is a commonly used Chinese herbal medicine and moxibustion raw material plant,so far has more than two thousand years of medicinal history,as one of the most commonly used Chinese medicinal materials.The incopat patent database was used to search the worldwide patent data of the last 20 years,and a total of 25279 argyi related patents were retrieved.The pattern of argyi patents was analyzed from the perspectives of global application trend,main technical fields,national economy composition,applicant ranking,patent value and other aspects by means of graph combination.The analysis shows that the innovation and development of argyi is in the stage of rapid development;The medical,Chinese patent medicine,cosmetics and physiotherapy of argyi are the hot research and development of current technology;There are a large number of patents related to argyi in the world,but they are mainly distributed in China and South Korea.Among them,the number of patents related to argyi in China reaches 20381,far higher than that in other countries,but the number of high-value patents is not very large,and the value and quality of patents are still insufficient compared with other countries.From the perspective of the current development trend of argyi,with the deepening of clinical application recognition and scientific research of argyi,there is a large patent space in the field of argyi.Patent applicants can formulate corresponding patent application strategies according to the global development opportunities,technological development status and existing weaknesses.
10.Effects of water extract and ethanol extract from the root of Caragana sinica on hyperuricemia in mice
Junjie ZHAO ; Jinjuan ZHANG ; Chunlei ZHANG ; Qinfeng ZHU ; Shanggao LIAO
China Pharmacy 2022;33(14):1694-1699
OBJECTIVE To investigat e the effects of water extract (WCS)and ethanol extract (ECS)from the root of Caragana sinica on hyperuricemia (HUA)in mice. METHODS Kunming mice were randomly divided into normal control group , model group ,allopurinol group (positive control ,5 mg/kg),benzbromarone group (positive control ,7.8 mg/kg),WCS low-dose , medium-dose and high-dose groups (38,75,150 mg/kg),ECS low-dose ,medium-dose and high-dose groups (50,100,200 mg/kg), with 10 mice in each group. Except for the normal control group ,the other mice were given potassium oxazinate intraperitoneally and hypoxanthine intragastrically for consecutive 7 d to establish HUA model. On the third day of modeling ,mice in each administration group were given corresponding drugs intragastrically ,and normal control group and model group were given equal volume of normal saline once a day for 5 consecutive days.The body weight of mice were weighted during administration ;one hour after the last administration ,the organ indexes of liver ,kidney and spleen were calculated ;the contents of serum uric acid (SUA), blood urea nitrogen (BUN)and serum creatinine (SCR);the activity of xanthine oxidase (XOD)in serum and liver tissue were determined. Relative mRNA and protein expressions of XOD in liver tissue ,relative expre ssions of GLUT9,URAT1 and OAT 1 in renal tissue were all detected ;and the pathological changes of renal tissue were observed. RESULTS There were no significant differences in liver index and spleen index in each group (P>0.05). Compared with normal control group , except for allopurinol group , there were no significant differences in the body weight and the contents of BUN and SCR in mice of other administration groups (P>0.05);the renal index and SUA content of mice in the m odel group and allopurinol group were significantly increased (P<0.05);in the model group ,the XOD activity in serum and liver tissue ,the relative mRNA and protein expression of XOD in liver tissue ,the relative expressions of GLUT 9 and URAT 1 protein in renal tissue were significantly increased (P<0.05),and the relative expression of OAT 1 protein in renal tissue was significantly decreased (P< 0.05). Compared with model group ,renal indexes of mice were decreased significantly in WCS and ECS groups (P<0.05),and the pathological damage of renal tissue was significantly improved ;SUA content ,XOD activity in serum and liver tissue ,the relative mRNA and protein expression of XOD in liver tissue ,and the relative expression of URAT 1 protein in renal tissue were decreased significantly in administration groups (P<0.05). The relative expression of GLUT 9 protein in renal tissue of mice in benzbromarone group and ECS high-dose group decreased significantly (P<0.05);relative expression of OAT 1 protein in renal tissue of mice in benzbromarone group ,WCS low-dose and high-dose groups ,ECS low-dose group were increased significantly (P<0.05). CONCLUSIONS WCS and ECS can significantly decrease the contents of SUA in HUA model mice ,and improve pathological state of renal tissue ,the mechanism of which may be associated with inhibiting XOD activity and uric acid reabsorption,and down-regulating protein and mRNA expression of XOD.