Objective To investigate the efficacy of wrist three-needle acupuncture in treating pre-adhesive stage scapulohumeral periarthritis. Method Seventy-eight patients with pre-adhesive stage scapulohumeral periarthritis were randomly allocated to two groups. The treatment group of 39 patients received wrist three-needle acupuncture and the control group of 39 patients, conventional acupuncture. Result After one course of treatment, the cure rate and the total efficacy rate were significantly higher in the treatment group than in the control group and there was a statistically significant difference (P<0.05). There was a statistically significant pre-/post-treatment difference in the VAS score in the treatment group (P<0.05). There was a statistically significant difference in the pre-/post-treatment difference value between the treatment and control groups (P<0.05). Post-treatment pain relief rate was significantly higher in the treatment group than in the control group (P<0.05). Conclusion Wrist three-needle acupuncture is even more effective than conventional acupuncture in treating pre-adhesive stage scapulohumeral periarthritis.

ObjectiveTo investigate the clinical efficacy of electrothermic acupuncture in treating knee osteoarthritis.Method One hundred and twenty patients were randomly allocated to treatment and control groups, 60 cases each. The treatment group received electrothermic acupuncture and the control group took Xianlinggubao capsules orally. The clinical therapeutic effects and changes in the WOMAC score were observed in the two groups after four weeks of treatment.ResultThe total efficacy rate was 95% in the treatment group and 85% in the control group. There was a statistically significant difference in clinical therapeutic effect between the two groups (P<0.05). There was a statistically significant difference in pre-/post-treatment WOMAC score difference value between the two groups (P<0.05).ConclusionThe clinical efficacy of electrothermic acupuncture is better than that of Xianlinggubao capsules in treating knee osteoarthritis with yang deficiency and cold congealment.

Objective To investigate the dose-effect relationship between Q-switch ruby laser and skin pigmentation in Guinea pigs. Methods Ten Guinea pigs were included in this study, and the back of each pig was divided into 5 areas, negative control area receiving no UVB irradiation or laser treatment, positive control area receiving UVB irradiation but no laser treatment, low-, middle- and high-dose areas receiving UVB irradiation followed by the treatment with laser at an energy density of 1.5, 3.0 and 6.0 J/cm2 for 4, 2, and 1 session, respectively, with an interval of 1 week. Melanin content index (MCI) was determined, and confocal laser scanning microscopy (CLSM) and histopathology were performed to observe melanin granules and melanocytes in the skin tissue from Guinea pigs before the initial laser treatment, 2 and 4 weeks after the final laser treatment. Results The best response was observed in the low-dose areas, and there was a complete clearance of hyperpigmentation in these areas, which maintained 8 weeks without recurrence, while different degrees of skin pigmentation recurred in the middle- and high-dose areas. Moreover, the low-dose areas showed a significantly lower melanin content index (MCI) compared with the middle- and high-dose areas (F = 4.2,P < 0.05), together with a marked reduction in the extent of pigment deposition, melanin granules, melanocyte number and density. Conclusions The efficacy of Q-switched ruby laser on skin pigmentation in Guinea pigs is correlated with the energy density and treatment frequency, and, low intensity and high frequency are often associated with a better treatment outcome.

ObjectiveTo compare the accelerating effect of topical recombinant human epidermal growth factor (rhEGF) and recombinant bovine basic fibroblast growth factor (rb-bFGF) on wound healing after fractional CO2 laser therapy.MethodsTwenty male guinea pigs were included in this study.After hair removal and irradiation with fractional CO2 laser,the back of each guinea pig was divided into 4 regions to be topically treated with rhEGF of 10 μg/cm2 (rhEGF group),rb-bFGF of 262.51 IU/cm2 (rb-bFGF group),the combination of rhEGF and rb-bFGF (combination group),or normal saline (control group),twice daily until the healing of wound.Skin physiology parameters including elasticity index and melanin index were detected before the irradiation,7,14 and 28 days after the irradiation,and compared between the 4 groups by analysis of variance.Tissue specimens were obtained from 4 mice at the above time points and subjected to pathological examination for the observation of collagen fibers and quantification of fibroblasts.ResultsAfter fractional CO2 laser therapy,the crusts fall off completely in growth factor-treated regions,while partly in the control regions,within 3 to 7 days; the wounds healed completely in 14 to 28 days in all the groups,with the regenerating tissue being more tender and redder compared with the surrounding unirradiated tissue.The wound surface was smaller in area and redder in color in the 3 growth factor-treated groups than in the control group.At 28 days after the irradiation,the elasticity index was 262.29 ± 62.40 in the combination group,202.00 ± 65.62 in the rhEGF group,188.86 ± 35.02 in the rb-bFGF group,167.14 ± 42.49 in the control group.Statistical difference was observed in elasticity index,but not in skin melanin index among the 4 groups.Pathological examination showed a dense and organized arrangement of collagen fibers in the combination group but a sparse and disorganized arrangement of collagen fibers in the control group.ConclusionThe combined application of rhEGF and rbbFGF can accelerate the healing of wound and increase the elasticity of regenerating tissue after fractional CO2 laser therapy.

Objective To explore the diagnostic value of SPECT/CT on fracture healing.Methods A total of 28 patients (13 males,15 females; average age 45.3 years) with fracture were enrolled.All underwent metal fixation.SPECT/CT scans were performed at least 6 months after the metal fixation.X-ray examination before SPECT/CT showed equivocal fracture healing.The fracture delayed union or nonunion were judged by degree of uptake as well as uptake pattern around fracture site.Final diagnosis was confirmed by surgery or follow-up X-ray film.Results Among 28 patients,15 showed delayed union (confirmed by follow-up),and 13 showed nonunion (confirmed by surgery).There were 13 screws in 6 patients showed abnormal uptake,suggesting screw loose (confirmed by surgery).Conclusions SPECT/CT imaging could be of clinical significance in assessing fracture healing.

Objective To speculate the precipitating factors,clinical features and histopathological changes in patients with granuloma annulare (GA).Methods A study was performed in 53 patients with GA . Results The incidence of female was more prevalent than male in our study. The exposure of sunlight was found more relatedly in GGA than LGA.The onset age of GGA was inclined to be elder and GGA seemed to last longer.The histopathological study showed that palisading granuloma pattern accounted for 71.7% in all patients,and scattered histiocytic infiltration accounted for 28.3%.Such as cryotherapy,topical steroids were used for LGA and systemic administration was an alternative to GGA. Conclusions Ultraviolet may be associated with the development of generalized GA. The most common pattern is the palisading granuloma though the histopathological changes were variable.Topical therapy is effective in LGA,while the systemic therapy is mainly used for GGA.

Objective To investigate the effect of rosiglitazone on the expr es sion of PPAR?,TGF-a1 and PCNA and its mechanism on renal interstitial fibrosi s following unilateral ureteral obstruction(UUO) in rat kidney. Methods Thirty r ats were randomly assigned to shame operation group(sham group),UUO group, rosig litazone(5 mg穔g-1?d-1) treatment group after UUO. Immunohistochemistry,RT-PCR,Western blotting were performed to investigate renal pathological changes an d examine the expression of PPAR?,TGF-a1,PCNA on the 7th and 14th day in the kidney. Results In comparison with the shame group,the expression of PPAR?,TGF-a1,PCNA of UUO and treatment groups increased significantly(P

OBJECTIVE:To probe into the cost control in PIVAS of our hospital in order to provide reference for the effective cost control.METHODS:The status quo of cost control of PIVAS was analyzed to provide corresponding countermeasures.RESULTS & CONCLUSIONS:The effective cost control can be achieved through improving cost accounting,controlling cost and reducing running cost and labour cost to promote the healthy development of PIVAS.

A polydimethylsiloxane ( PDMS )/glass microfluidic chip consisting of a three-layer sandwich structure and three-parallel micro-electrode system was fabricated for the separation of polystyrene microspheres according to the particle size in high conductive solution by electric field produced by alternating current. The principle of electrokinetics of microspheres directional movement was investigated. The results showed that, when the applied voltage was 14 V at 100 kHz, the separation efficiency of 10 and 25 μm polystyrene microspheres was the best. Similarly, with a voltage of 10 V at 2 MHz, the separation efficiency of 5 and 25 μm polystyrene microspheres could achieve the highest value. Meanwhile, the voltage of 11 V at 1 MHz was suitable for the separation of 5 , 10 and 25 μm polystyrene microspheres with the separation efficiency of over 90%. At the same time, the formation of the laminar region in the middle of the electrode gap was the key role of microsphere separation.

ObjectiveTo study the roles of aquaporin 3(AQP3),Caspase 14 and bleomycin hydrolase(BH) in skin barrier in patients with chronic eczema and to tackle the mechanisms underlying the therapeutic effect of narrow-band ultraviolet B(NB-UVB) on eczema.MethodsTransepidermal water loss (TEWL)and water content of skin were detected by a muhi-functional skin tester in lesions and non-lesional skin of 30 patients with chronic eczema.The protein expressions of AQP3,Caspase 14 and BH were detected by immunohistochemistry in tissue specimens from lesioual and non-lesional skin of these patients.Cultured keratinocytes were irradiated with 3 different doses(400,800,1200 mJ/cm2) of UVB,and Western blot was performed to quantify the protein expressions of AQP3,Caspase 14 and BH at 24 hours after the irradiation.ResultsThere was a significant decrease in water content of skin(11.32 ± 5.25 vs.49.68 ± 8.62) but an increase in TEWL (86.28 ± 16.35 vs.14.62 ± 6.68) in lesional skin compared with non-lesional skin from the patients with chronic eczema(both P ＜ 0.05).The protein expression levels of AQP3,Caspase 14 and BH in lesional skin were 5.3 times,4.2 times and one-third of,those in non-lesional skin from the patients,respectively,and significant differences existed between the lesional and non-lesional skin in the 3 parameters (all P ＜ 0.05 ).After irradiation with NB-UVB at 400,800 and 1200 mJ/cm2,the expression level of AQP3 in keratinocytes was decreased by 52％,77％ and 84％,respectively,and that of Caspase 14 was decreased by 18％,22％ and 78％,respectively,compared with unirradiated keratinocytes (all P ＜ 0.05).A marked decrease(by 66％) was also observed in the expression of Caspase 14 in keratinocytes after irradiation with NB-UVB at 1200 mJ/cm2(P ＜ 0.05).ConclusionsThe impairment of skin barrier function in chronic eczema may be related to the downregulated expression of BH and upregulated expression of Caspase 14 and AQP3.The therapeutic effect of NB-UVB on chronic eczema may be partly attributed to the diminished expression of AQP3,Caspase 14 and BH.