ObjectiveTo analyze the different effects of spiral progress method and traditional method used in computer teaching in TCM colleges and universities.MethodsStudents were divided into the groups of spiral and traditional. At the end of the course, students would have a course examination.Results Students in spiral group got better scores than students in traditional group (P<0.05).Conclusion Using spiral progress method in computer teaching in TCM colleges and universities can help students develop the right thinking model and scientific learning method. It can also improve students’ ability to use computer knowledge in TCM study and research.

Objective To speculate the precipitating factors,clinical features and histopathological changes in patients with granuloma annulare (GA).Methods A study was performed in 53 patients with GA . Results The incidence of female was more prevalent than male in our study. The exposure of sunlight was found more relatedly in GGA than LGA.The onset age of GGA was inclined to be elder and GGA seemed to last longer.The histopathological study showed that palisading granuloma pattern accounted for 71.7% in all patients,and scattered histiocytic infiltration accounted for 28.3%.Such as cryotherapy,topical steroids were used for LGA and systemic administration was an alternative to GGA. Conclusions Ultraviolet may be associated with the development of generalized GA. The most common pattern is the palisading granuloma though the histopathological changes were variable.Topical therapy is effective in LGA,while the systemic therapy is mainly used for GGA.

Objective:To explore and discuss the effect of Xiaopi Yueyi formula on behavioral changes in rats with chronic fatigue syndrome.Methods:24 rats were divided into group model(16 rats) and control(8 rats) at random,and after produced rats with chronic fatigue syndrome,half in the former were taken orally with Xiaopi Yueyi formula while the latter were taken equally normal saline,moreover,methods of Open-Field、tail suspend time and exhaust swimming time were estimated and evaluated.Results:After succeeding in chronic fatigue manifestation in rats of group model,number of parallel and vertical movement diminished markedly,furthermore,time of tail suspend and exhaust swimming prolonged apparently.Nevertheless,Xiaopi Yueyi formula could ameliorate significantly these parameters in rats(P

AIM:To investigate the role of miR-125b in regulating the sensitivity of CD133+ colorectal cancer cells to cisplatin.METHODS:The expression of miR-125b was detected by RT-qPCR in the routine SW480 cells and CD133+ SW480 cells.Flow cytometry analysis was performed to measure the percentage of CD133+ cell population in the SW480 cell line treated with miR-125b and cisplatin.MTT assay was performed to evaluate the effect of miR-125b on the cisplatin-induced cell death in the CD133+ SW480 cells.Bioinformatics and Western blot were performed to determine whether the expression of HAX-1 was regulated by miR-125b.JC-1 staining, Annexin V staining and Western blot analysis were used to study the pathway of apoptosis in the CD133+ SW480 cells co-treated with miR-125b and cisplatin.RESULTS:The expression of miR-125b was significantly lower in the CD133+ SW480 cells than that in the routine SW480 cells and normal colonic epithelial FHC cells.Treatment with cisplatin alone increased the percentage of CD133+ SW480 cell population.However, miR-125b significantly inhibited the enrichment of CD133+ cell population induced by cisplatin.In addition, the results of MTT assay showed that the anti-tumor effect of cisplatin was significantly enhanced when the miR-125b was transfected into the CD133+ SW480 cells.The results of Western blot indicated that the HAX-1 gene was the target of miR-125b.Furthermore, the apoptosis induced by the combination of miR-125b and cisplatin was dependent on the dysfunction of mitochondrial membrane, leading to the release of cytochrome C into the cytoplasm and the subsequently activation of apoptosis in the CD133+ SW480 cells.CONCLUSION:miR-125b increased the sensitivity of CD133+ colo-rectal cancer cells to cisplatin by down-regulating the expression of HAX-1.

Objective To observe the changes of cardiac rhythms in a swine model of adult asphyxia! cardiac arrest. Method Sixteen Pigs were aphyxiated by endotracheal tube clamping until 8 min after loss of aortic pulsations. Resuscitation was then provided and swinds were assigned to received 0.045 mg/kg epinephrine intravenously after 3 min of basic life support. The animals with restoration of spontaneous circulation within 20 min from CPR were defined as successfully resuscitated, while the rest were identified as unresuscitation. Electrocardiogram ( EGG) were monitored from the start of asphyxia to the start of the CPR. Results When loss of pulsations occurred, 2 of 16 animals had ventricular fibrillation; 10 pigs exhibited pulseless electrical activity, and 4 pigs had asystole. During the 8 min after the loss of aortic pulsations, pulseless electrical activity converted to VF in 7 pigs. Immidiatedly prior to resuscitation, VF occurred in 9 pigs, asystole in 4 pigs, and PEA in 3 pigs. Conclusions Most of animals in this swine model of asphyxial cardiac arrest presented PEA, but most of them converted to VF especially late in the asphyxial process.

In the first case, a 15-year-old girl presented with recurrent multiple erythematous edema,bullae and ulceration on the face and extremities for 3 years, which had developed into plaques and nodules on the face and trunk for 6 months. Histology revealed angiocentric and angiodestructive infiltrates with medium-sized atypical lymphoid cells positive for LCA, CD45RO, CD56 and EBV staining throughout the dermis. The patient was diagnosed with extra NK/T-cell lymphoma, nasal-type. She subsequently had a rapid downhill clinical course with resistance to systemic chemotherapy, and died one month later. In the second case, a 44-year-old male was admitted to the hospital with progressive infiltrated mass on the right waist for 1 year, and a 4-month-history of lymphadenectasis. Histologically, there was a massive and dense infiltrate with middle and large-sized, CD4 and CD56-positive lymphoblastics throughout the dennis and subcutaneous tissue. A diagnosis of blastic NK-cell lymphoma was made. The patient was managed with surgical excision followed by systemic chemotherapy. He had been followed up and free of relapse till the time of this writing.CD56 positive cutaneous lymphoproliferative disorders appear to be highly invasive.

Objective To assess the clinical features and diagnostic index of progressive macular hypomelanosis(PMH).Methods Eight patients with PMH were recruited into this study.Wood's lamp and in vivo confocal laser scanning microscopy(CLSM)were utilized to observe the lesions of all patients.Microbiological culture of lesion specimens from 2 patients was performed.Tissue specimens from 4 patients underwent immunohistochemieal staining with anti-S-100 and anti-TRP-1 antibodies for the detection of melanocyte quantity.Electron microscopy wag utilized to observe ultrastructural features of lesions.Primary culture of melanocytes was carried out with lesional epidermis.Resnits Under Wood's lamp.the lesions of PMH showed punctiform red fluorescence.CLSM revealed complete pigmented tings in lesions with decreased melanin granules compamd with those surrounding normal skin.Microbiological culture grew red fluorescence-producing,gram-positive bacillus which was identified as Propionibacterium acnes.Immunohistochemistry showed no significant difference in the number of S-100-postive cells or TRP-1-positive cells per high power field (× 400)between lesions and surrounding normal skin (8.25±0.96 vs 8.75±1.71,4.25±0.96 vs 4.50±1.29,both P>0.05).Ultrastructural studies showed a large reduction in the number of type Ⅳ melanosomes in lesions of PMH,along with numemus membrane bound bodies and clusteredly distributed,small type Ⅱ-Ⅳ melanosomes.Melanocytes,with morphological similarity to normal melanocytes,were successfully isolated from the lesional tissue,cuhured and identified.Conclusion A primary diagnostic criteria is pro-posed for PMH according to the clinical and experimental studies.

Objective To analyze the clinical and laboratory phenotypes of Duchenne muscular dystrophy(DMD)in a retrospective study of 96 cases and to evaluate therapeutic efficacy of glucocorticosteroid.Methods The clinical phenotype,laboratory examinations resuhs and the records in the follow-up in 96 patients with DMD were collected.The level of serum creatine kinase(CK)and motor ability before and after glucocorticosteroid therapy were analyzed by statistical analysis.Their myocardium impairments and intelligence conditions were also assessed.Results(1)The level of serum CK (mmol/L)had three peaks at the age of ≤ 3 years old(16 547.9 ±770.9),5 years old(14 371.9 ± 696.7)and 8 years old(13 089.8 ± 877.6).The CK level significantly decreased after dexamethasone (5-10 mg,iv)treatment for 10-15 days,but increased again after prednisone acetate(0.50-0.75 mg · kg-1 · d-1,oral)administration for one month(F =6.758,P =0.003).(2)The motor ability improved in 51 DMD cases with long-term oral admission of prednisone,including 24 cases receiving repeated dexamethasone,iv.(3)The myocardial perfusion imaging in 37 DMD cases showed remarkable uneven “spotted like” radionuclide distribution in ventricle.(4)The intelligence quotients in 24 DMD were lower than normal population.Conclusions There are high CK hyperlipidemia and myocardial damage in the sub-clinical stage of DMD and myocardium impairment is positively correlated with age.Glucocorticosteroid therapy has an important effect on the protection of motor and cardiac functions,with recommendation of using in early stage of disease.

A 27-year-old man presented with a 3-year history of persistent asymptomatic papules on the left chest and axirlary fossa. Multiple skin biopsies were performed and histopathology revealed mild acanthosis, extension of the dermal papilla, lichenoid lymphoid infiltrates in upper dermis. Some lymphoid cells migrated into the epidermis and formed Pautrier's microabscesses. Immunohistochemistry revealed that the infiltrating cells were positive for LCA, CD45RO, CD3, CD4 and CD8 (scattered), but negative for CD68 or CD30. Cutaneous laser confocal microscopy showed the shadow of scattered, oval or round, slightly refractive cells measuring 4-8 pm in diameter. A diagnosis of papular mycosis fungoides was made. The papules were softened with the lightening of lesional color after treatment with narrow-band ultraviolet B, topical fluticasone propionate cream and isotretinoin gel.