Objective To observe the therapeutic effect and effects on nerve conduction velocity of α-lipoic acid combined with monosialoteterahexosyl ganglioside sodium (GM 1) on diabetic peripheral neuropathy (DPN).Methods Ninety-six cases patients with DPN were randomly divided into the observation group (50 cases) and the control group(46 cases).The control group received GM1 40 mg daily though travenous drip,the observation group received α-lipoic acid 600 mg and daily though travenous drip.The course of treatment was 2 weeks in two groups.The effect was observed after 2 weeks,the motor nerve conduction velocity (MNCV) and sensory nerve conduction velocity (SNCV) of median nerve and common peroneal nerve were detected by EMG.Results The total effectiveness of observation group was 94.00％(47/50),higher than that of the control group(71.74％ (33/46),x2=7.0205,P＜ 0.05).After the treatment,MNCV and SNCV of median nerve of observation group and the control group were (47.6813.34)m/s and (43.82±3.10)m/s,(40.23±2.76)m/s,(37.31 ±2.79)m/s,and M NCV and SNCV of common peroneal nerve of observation group and the control group were(42.86±3.16)m/s and (39.47±2.17)m/s,(35.67±2.61) m/s and (32.16±2.50) m/s,all improved compared to before treatment(observation grou:t =7.876,8.428,6.923,7.234;control grou:t =3.572,3.483,3.413,4.182;P＜0.05),and the magnitude of improvement of the observation group was more significant,and the difference was significant compared to control group after six months (t =5.133,3.827,4.634,4.545;P＜0.05).Conclusion α-lipoic acid combined with GM1 in the treatment of DPN can significantly improve the clinical symptoms of patients and improve nerve conduction velocity,is better than the single use of gangliosides,it is worthy of clinical application.

Objective To explore the change of peripheral benzodiazepine receptors (PBR) expression in the telencephalon of mice with schistosomiasis. Methods The changes of PBR mRNA in the telencephalon of schistosomiasis mice were observed by the real-time FQ-PCR method. The results were judged with circle number of times (C_T) when the level of amplification exponent achieved detecting liminal value. Results Comparing the experiment group (n=10) with the healthy control (n=10) at the 8th、12th、20th week, the changes of C_T were significant(P

Objective To evaluate the value of transient elastography (FibroScan,FS)in predicting hepatic fibrosis in patients with HBeAg-negative chronic hepatitis B (CHB).Methods One hundred and four patients with HBeAg-negative CHB,who were diagnosed and treated in Hubei Hospital of Traditional Chinese Medicine from June 2011 to May 2013,were enrolled in this study.All patients underwent FS for liver stiffness measurement before liver biopsy.Statistical analysis was applied to compare liver stiffness (kPa)with fibrosis stage (de-termined by liver biopsy).The receiver operating characteristic (ROC)curve of FS was constructed,and the area under the ROC curve (AUC)was calculated to analyze the accuracy of live stiffness in predicting significant fibrosis and cirrhosis.Comparison between groups was made by Kruskal-Wallis H test,followed by Mann-Whitney U test for multiple comparisons.The correlation between two variables was an-alyzed by Spearman rank and Pearson correlation test.Results Liver stiffness gradually rose as the degree of hepatic fibrosis increased, with significant differences between groups (P<0.01 or P<0.05).Liver stiffness was positively correlated with the stage of hepatic fibrosis (r=0.810,P<0.01).The AUC of liver stiffness for detecting liver cirrhosis was 0.956;the cut-off value for diagnosing liver cirrhosis was 13.1 kPa;the sensitivity was 92.7%,and the specificity was 80%.Conclusion FS is a promising noninvasive method for the assessment of hepatic fibrosis in patients with HBeAg-negative CHB;particularly,it has high accuracy in the diagnosis of liver cirrhosis.FS combined with direct or indirect markers may play an important role in differential diagnosis and efficacy evaluation in patients with hepatic fibrosis.

The control meaurees and endemic situation were analysed in Dali Prefecture,Yunnan Province. The infection rates of population and livestock decreased from 6.42% and 3. 81% in 2004 to 0.07% and 0.15% in 2008, respectively. No infected snail and acute schistosomiasis case were discovered in 2008. The proportion of villages with serious endemic situation decreased from 49.42% in 2004 to 7.51% in 2008, and 92.48% of villages reached the criteria of transmission control or transmission interruption. The endemic situation and snail status of Dali Prefecture have decreased significantly after the implementation of the comprehensive control project, but the consolidation and enlargement of the control result still remains a challenge.

Objective To discuss the comparability of blood glucose(Glu)results with different detection systems.Methods Six different kinds of biochemistry detection systems were used to detect plasma Glu concentrations at 2 levels of Randox quality controls and 48 clinical plasma according to EP9-A file.The collected data were treated with statistical analysis.Results Analysis of variance showed Glu results from different control and patients plasma had significant difference between various detection systems (P

Objective To explore the influence of ozone therapy on high-sensitivity C-reactive protein (hs-CRP) and matrix metalloproteinases-3 (MMP-3) in synovial fluid of patients with knee osteoarthritis,and to investigate the possible mechanism.Methods Sixty-two patients with knee osteoarthritis were divided into observation group and control group by random digits table method with 31 cases each.The patients of observation group were treated with ozone,while the patients of control group were treated with hyaluronic acid once a week for 5 weeks.The efficacy was evaluated and the level of hs-CRP and MMP-3 in synovial fluid were detected before and after treatment.Results The total effective rate in observation group was 100.00％ (31/31),significantly higher than that in control group [87.10％ (27/31)],and there was significant difference (x2 =4.28,P ＜ 0.05).After treatment,the level of hs-CRP and MMP-3 in observation group was (6.68 ±2.15) mg/L,(69.84 ± 16.97) μ g/L,significantly lower than that in control group [(8.72 ± 2.39) mg/L,(85.43 ± 19.52) tμ g/L],and there was significant difference (t =3.53,3.36,P ＜ 0.01).Conclusion There is significant effect of ozone therapy of patients with knee osteoarthritis,its mechanism may be reducing the level of MMP-3 and inhibiting inflammatory reaction.

Objective To compare the ultrasonographic manifestations of ligamenta patella and their entheses of healthy volunteers with those of professional athletes. Methods Sonography was performed using a GE Logiq 700 MR Expert Series ultrasound equipment and a 5-10 MHz multifrequency linear array transducer. Fifty-two healthy volunteers and 20 professional athletes underwent sonography on ligamentum patella of both sides. Results The normal ligamenta patella′s contour was regular and sharply defined, appearing as tightly packed echoic structures with fine parallel internal linear echoes with a synovial sheath to a thickness of 0.02 cm. The attaching bone surface was smooth and continuous. The ligamenta patella of male was longer than that of the female(P

Objective To study the clinical value of three-dimensional vascular reconstruction of power Doppler imaging in monitering acute rejection of renal transplant.Methods Eighty-eight renal allograft patients were studied by three-dimensional vascular reconstruction and graded the vascular flow.Results The ratio of successful renal transplant three-dimensional vascular reconstruction reached 95.5% in 88 cases.Blood flow gradation was 4-5 levels in 30 controls and 0-4 levels in 58 patients with acute renal transplant rejection. Blood flow gradation was closely relevant to the time when the blood flow began to improve and the renal function began to restore after treatment, value of r being -0.9224 and -0.9223 respectively (P

Objective To study the effect and mechanism of electroaeupuncture at acupoint Guanyuan on expression of brain-derived neurotrophic factor (BDNF) and tropomyosine receptor kinase B (TrkB) in spinal cord and excitability of detrusor of rat with urinary retention after spinal cord injury. Methods The model of urinary retention after spinal cord injury was established by using weight drop method. Eighty female Sprague-Dawley rats were randomly divided into four groups, namely, a normal, a sham, a model and an electroacupuncture (EA) group, with 20 rats in each group. The EA group was given electroacupuncture at aeupoint Guanyuau once a day for 10 days in total, while the other groups was given no treatment. After 10 days, the expression level of BDNF and TrkB in spinal cord of injury site was measured by immunohistochemistry staining, and excitability of detrusor was measured by using the muscle strips in vitro. Results It was revealed that, with regard to the expression level of BDNF and TrkB in spinal cord,the model group exceeded the normal and sham group, the EA group significantly exceeded the other groups ( P < 0. 05 ). With regard to the excitability of detrusor, the model group was significant-ly lower than the normal and sham group. The EA group significantly exceeded the model group ( P < 0.05 ). Conclusion Electroacupuncture at acupoint Guanyuan can raise the expression level of BDNF and TrkB in spinal cord and increase the excitability of detrusor in rats with urinary retention after spinal cord injury.