Objective The proliferation and differentiation of neural stem cells in the subventricular zone(SVZ) and subgranular zone(SGZ) have been observed in normal old rats or treated by middle cerebral artery occluded(MCAO) and sham operation respectively. Methods Old rats were used to make models of middle cerebral artery occluded(MCAO).By BrdU pulse labeling and immunohistochemical single staining,the change of the number of BrdU positive cells was observed in SVZ and SGZ of normal rats or cerebral ischemia rats after MCAO 3d,7d,14d,21d,28d and sham operation respectively.By BrdU accumulating label and immunohistochemical double staining,BrdU/NeuN and BrdU/GFAP positive cells were determined in SVZ and SGZ of aged rats after MCAO 14d. Results BrdU positive cells were found in bilateral SVZ and SGZ of all groups above mentioned.Compared with normal and sham groups,BrdU positive cells of SVZ and SGZ in cerebral ischemia rats were strikingly increased.BrdU positive cells reached the peaks in SVZ at 7d and in SGZ at 14d after MCAO.At 14d after MCAO,BrdU/NeuN(0.98%) or BrdU/GFAP(12.56%) double labled cells were observed in SVZ,but no double labled cell was observed in SGZ.Conclusion The focal cerebral ischemia markedly stimulated proliferation of neural stem cells in SVZ and SGZ in old rats,and some proliferative cells in SVZ can differentiate into neurons or glial cells.

A novel method based on independent component analyzing (ICA) in frequency domain to distinguish the frequency characteristics of multi-sensor system is presented. The conditions of this type of ICA are considered and each step of resolving the problem is discussed. For a two gas sensor array, the frequency characteristics including amplitude-frequency and phase-frequency are recognized by this method, and cross-sensitivity between them is also eliminated. From the principle of similarity, the recognition mean square error is no more than 0.085.

Objective To correct the nonlinear error of sensor output, a new approach to sensor inverse modeling based on Back-Propagation Fuzzy Logical System (BP FS) is presented. Methods The BP FS is a computationally efficient nonlinear universal approximator, which is capable of implementing complex nonlinear mapping from its input pattern space to the output with fast convergence speed. Results The neuro-fuzzy hybrid system, i.e. BP FS, is then applied to construct nonlinear inverse model of pressure sensor. The experimental results show that the proposed inverse modeling method automatically compensates the associated nonlinear error in pressure estimation, and thus the performance of pressure sensor is significantly improved. Conclusion The proposed method can be widely used in nonlinearity correction of various kinds of sensors to compensate the effects of nonlinearity and temperature on sensor output.

Objective To explore the effect of flow yoga on balance ability and fear of falling of community patients with stroke.Methods Divided 92 community patients with stroke into the intervention group (46 cases) and the control group (46 cases) randomly,routine community nursing cares were used in the control group,while the flow yoga were used in the intervention group in addition.The berg balance scale,single direct question of fear of falling and modified falls efficacy scale were used to evaluate balance ability and fear of falling after the intervention between the two groups.Results The patients' balance ability was (48.28±10.64) in the intervention group,which was significant higher than that of in the control group (32.04±11.42),t=6.316,P＜0.05.The fall efficacy in the intervention group was (132.03±27.38),which was significant higher than that of in the control group (108.74±25.68),t=8.256,P＜0.05.The rate of fear falling in the intervention group was 26.09％(12/46),which was significant lower than that of in the control group (32.16T％,15/46),x2=6.545,P＜0.05.Conclusion Flow yoga can improve stroke patients' balance ability and reduce fear of falling.

Objective To study the morphology of the persistent foramen of Huschke (PHF)and the weakness of the remains of foramen of Huschke (WRHF)in the external auditory canal (EAC)by high resolution computer tomography (HRCT)in vivo. Methods The thin HRCT images of 525 cases elder than 5 years were retrospectively reviewed by two radiologists,to detect the PHF or WRHF,and to depict the precise location,size and the distance to the tympanic membrane.The incidence,sex differences, and morphologic data of PHF and WRHF were statistical analyzed.Results ①PHFs were found in 146 EACs of 40 males and 77 fe-males with the prevalence of 13.91%,and were found on both sides in 29 cases.Mean maximum axial diameter was 2.35 mm± 1.1 6 mm (0.6-5.8 mm)and sagittal diameter was 2.09 mm±1.2 mm (0.5-6.2 mm).Mean distance between the inner edge of PFH and the sulcus tympanicus was 1.61 mm±2.4 mm (0-10 mm).②WRHFs were found in 233 EACs of 58 males and 122 fe-males with the prevalence of 22.1 9%,and were found on both sides in 53 cases.Mean maximum axial diameter was 2.43 mm± 0.89 mm (0.8-5.4 mm)and sagittal diameter was 2.08 mm±0.73 mm (0.6-4.3 mm).Mean distance between the inner edge of WRPFH and the sulcus tympanicus was 1.55 mm±1.2 mm (0 - 9.9 mm).③The distance to the sulcus tympanicus was within 3 mm in 137 cases (90.73%)of PFHs and 221 cases (94.43%)of WRFHs.Conclusion Both PHF and WRFH are common by the CT morphological study in vivo.The medial part of EAC is predominant and female is predominant.The incidence and the location of PHF and WRFH are no significant difference.

Objective:To investigate the inhibitory effect of Epibueropyridinium A on aldose reductase.Methods: Aldose reductase was extracted from cattle crystalline lens.Besides Epibueropyridinium A,the reactive system also contained DL-glyceraldehyde,aldose reductase,and NADPH.The activity changes of aldose reductase were detected at 340 nm.Epalrestat was taken as the positive control.The inhibitory type,Ki and IC_(50) were determined by double reciprocal plot,quadratic drawing,and drawing of inhibitor's concentration to inhibitory ratio,respectively.Results: Epibueropyridinium A significantly inhibited the activity of aldose reductase in a competitive manner,with IC_(50) being 4.2 ?g/ml and Ki being 4.88 ?g/ml.Conclusion: Epibueropyridinium A is competitive inhibitor of aldose reductase.

Objective To study and evaluate the diagnostic procedures and the treatment strategy of adrenal tumors. Methods The accurate rate of localization and qualitative diagnosis of various imaging procedures for 117 cases of adrenal tumors were analyzed and the treatment efficacy studied. Results The accurate localization rate of the tumors was 84.6% for B ultrasonography,90.6% for CT,98.1% for MRI and the accurate qualitative diagnosis rate 39.2%,69.6% and 79.6% respectively.107 of the 117 cases were surgically cured,of which 30 were considered as large tumors with a volume exceeding 10 cm?5 cm?4 cm,the largest being 20 cm?15 cm?10 cm.9 patients underwent intervention therapy and no surgical procedure has been undertaken in 1. Conclusions Imaging procedures are very helpful for the localization and qualitative diagnosis of adrenal tumors.B ultrasonography can be used as a screening procedure.CT and MRI are high in accurate localization rate.As to the accurate qualitative diagnosis rate MRI is a little higher than CT.Accurate surgical incision is of prime importance for a surgical success.Adequate perioperative preparation can minimize complications to occur.Laparoscopic ultrasonography should be on schedule whenever laparoscopic adrenalectomy is contemplated.

Objective To investigate the relationship between different behavior types with classification of TCM and emotional disturbance in coronary artery disease (CAD) patients. Methods Two hundred and forty-two CAD patients were investigated and grouped to A or B type behavior with type A behavior pattern questionnaire (TABPQ) and symptom check-list 90 (SCL-90). To analysis the emotional disturbance in two groups and investigate their relations with syndrome classification of TCM. Results SCL-90 factor score of anxiety and depression in CAD patients was obviously higher than Chinese norm. Factor score of depression, anxiety, hostility, phobia, paranoid and psychotic state in A-type behavior group was obviously higher than that in B-type group, with statistical significance (P

Objective To observe the clinical curative effect of transcatheter hepatic arterial chemoembolization (TACE) in treatment of gastrointestinal stromal tumors(GIST) with metastatic liver cancer. Methods Four GIST patients with 19 lesions of liver metastasis were treated with 2 ～ 4 times of TACE. The diameters of the metastatic tumor were from 2 ～ 5 cm. FuDR, CDDP, EPI-ADM, together with lipiodol ultrafluid and gelatin sponge to from a mirture was prepared for arterial embolization. Results DSA showed abundant tumor vasculature and tumor stain on hepatoarterial angiography. The size of tumor showed no decrease after TACE and lipiodol was cleared away in a short period. No patient achieved CR or PR until two of them received imatinib. Conclusions TACE is not effective in treating hepatic metastasis from GIST shown in limited cases. (J Intervent Radiol, 2006, 15: 283-285)

BACKGROUND: Recently, researchers separate neural stem cells (NSCs) from neural system. As the progenitor of nerve cell and glia cells, NSCs have strong ability of self-renewal and proliferation, and play a very important role in repair process after nerve injury. OBJECTIVE: To review the repairing effect of NSC transplantation on hypoxic-ischemic encephalopathy. RETRIEVAL STRATEGY: Using the "Neural stem cells, brain, hypoxic-ischemic", we searched PubMed database for related articles published in English between 1993 and 2007. 131 articles were collected, and after the first trial, only articles ① related to neural stem cell transplantation for brain injury, and ②published in recently or in authoritative journals were selected. Repetitive researches were excluded. LITERATURE EVALUATION: The articles were about the studies of NSCs and randomized and controlled experiment for hypoxic-ischemic brain injury. Thirty-three English articles were included involving the biological characteristics of NSC, distribution, separation and culture in vitro, and the basic experiment or clinical research about the repair of hypoxic-ischemic brain injury by NSC. Of these articles, there are 7 review articles, and the others were about clinic or basic experiment researches. DATA SYNTHESIS: Researches show that after transplantation NSC can survive in host, furthermore, it proliferates and differentiates to repair the configuration and function of injured brain area. Even though we have achieved much in NSC transplantation for brain injury, yet there are many problems to be resolved, such as NSC source and conservation, the control mechanism of differentiation and proliferation when NSC is cultured in vitro. In addition, the amount or functional activity of NSCs acquired is lower, and the molecular biological features of cells in repaired tissue are uncertain. CONCLUSION: As a kind of ideal seed cell, NSC provides an approach for the repair of hypoxic-ischemic brain injury, and starts a new era of clinic therapy by stem cells.