Objective To evaluate a blood test,UniCAP system(screening IgE to food or inhalant allergens,eosinophil cationic protein,ECP)in differentiation the capability of lgE-mediated allergic diseases in children. Methods 26 atopic dermatitis,33 asthma/bronchiolitis,20 allergic purpura, 15 Kawasaki disease patients and 20 normal children were tested total lgE,lgE to food or inhalant allergens,ECP by UniCAP system and compared.Resuits Fx5E positive rate are 73.1%,24.2%,25.0%,13.3%in atopic dermatitis,asthma,allergic purpura,Kawasaki disease respectively,and the Phadiatop positive rate are 23.1%、69.7%、10.0%、6.6%.The serum levels of ECP、TIgE of patients were higher than the mormal controls,with the highest level in asthma, and lowest in Kawasaki disease,the differences were significant(P<0.05).Conclusions Food orinhalant allergens plays an important role in the inflammatory process of asthma,atopic dermatitis,and some role in allergic purpura,Kawasaki disease.Food allergens are more important to atopic dermatitis.and inhalant allergens are more important to asthma.

Objective To analyze the family and clinical characteristics of talking about killing self (TAKS) among children and adolescents and its relationship with social competency.Methods One thousand nine hundred and seventy-five 7-17-year-old students from 12 schools (each four for primary,middle and high schools) of Wuhan area,were approached through multistage random cluster sampling,and administered with a self-designed general information questionnaire and the Chinese version of Children' s Depression Inventory.In addition,subjects' parents completed Achenbach' s Child Behavior Checklist (CBCL).TAKS was judged based on item 91 (talk about killing self) of CBCL.Multiple Logistic Regression was used to identify factors associated with TAKS.Results The half-year prevalence rate (95％ CI) of parent-reported TAKS was 4.1％ (3.2％,5.0％).TAKS was significantly associated with bad marital status of parents (OR=2.29),unemployment of fathers (OR=2.07),frequently jangling between father and mother (OR=2.23),parents ever having fought with each other intensely (OR=2.00),being frequently bullied by classmates (OR=2.11),internal behavior problem (OR=2.28),external behavior problem (OR=2.50) and depressive symptoms (OR=4.83).TAKS subjects had significantly lower social compctency score than non-TAKS subjects((18.8±7.6) vs.(20.9±6.9),P=0.007).Conclusion The prevalence of TAKS is higher among children and adolescents with several disadvantaged family factors,behavior problems and depression.Children and adolescents with TAKS also have worse social competency.More close clinical attention should be paid to such phenomenon.

Objective To investigate the safety of different intensity anticoagulation therapy of warfarin in preventing thromboembolism in octogenarian patients with nonvalvular atrial fibrillation (NVAF). Methods The 130 patients with persistent or permanent NVAF were randomly divided into three groups: low-intensity warfarin group (35 cases, international normalized ratio, INR (1.5-2.0), moderate-intensity warfarin group (32 cases, INR 2.1-2.5) and aspirin control group (63 cases). The rate of hemorrhagic events and the effect on renal function were observed. Results The incidence of hemorrhage was the lowest in low-intensity warfarin group compared to the other groups with slight bleeding in one case. life-threatening bleeding in one case, severe bleeding in one case and slight bleeding in four cases occurred in moderate-intensity warfarin group. Life-threatening bleeding in three cases, severe bleeding in two cases and slight bleeding in six cases occurred in aspirin control group. There were significant differences in bleeding incidence among the three groups (χ2=5.13,P＜0.05). The low-intensity warfarin group and moderate-intensity warfarin group were superior to the aspirin control group in the effect on renal function (P＜0.05). Conclusions It is safe that the dose of warfarin is maintained at low anticoagulation intensity between INR 1.5 and 2.0 in octogenarians with NVAF.

Objective To explore the relationship between depression and anxiety and peripheral blood cell counts ( white blood cell ( WBC) ,neutrophil ( NEU) ,lymphocyte ( LYM) ,red blood cell ( RBC) and hemoglobin ( HGB) ) of heroin?dependent patients receiving methadone maintenance treatment ( MMT) . Methods Two hundred and fifty?two MMT heroin addicts, who had received peripheral blood tests within the week prior to the study,were administered with a general information questionnaire,SDS and SAS. The results of blood tests of these patients were compared with those of 109 healthy people in physical examina?tion. Multiple linear regression was used to analyze associations between indicators of peripheral blood cell counts and depressive and anxiety symptoms. Results Compared with healthy controls,MMT patients had significantly higher proportions of elevated WBC(14.7%vs 3.7%),NEU(12.7% vs 2.8%) and LYM(9.9%vs 2.8%) counts (all P<0.05),and low RBC(10.3%vs 4.6%) counts and HGB(11.5%vs 4.6%) concentra?tion (both P<0.05). Multivariate linear regression analysis showed that blood cell counts of WBC ( β=0.261, P<0.01),NEU ( β=0.264, P<0.01),LYM ( β=0.196, P=0.003) and RBC ( β=-0.144, P=0.021) were independently associated with depression;and blood cell counts of WBC ( β=0.298, P<0.01) and LYM ( β=0.269, P<0.01) were independently associated with anxiety. Conclusion Improving the in?flammatory and malnutrition status may be helpful for reducing depressive and anxiety symptoms of MMT her?oin addicts.

Objective:To prepare capsaicin liposomes and study the feasibility by in vitro percutaneous penetration test. Meth-ods:Capsaicin liposomes were prepared by a film-ultrasonic method. The best formula was screened by orthogonal test based on single factor studies with the entrapment efficiency as the index. The improved Franz diffusion cells were used to study the transdermal pene-tration of capsaicin suspensions, capsaicin liposomes and capsaicin ointments, and the cumulative penetration amount through the isola-ted rat skin was compared. Results:The optimal formula of capsaicin liposomes were as follows:the ratio of capsaicin to lipids was 1∶5;the amount of Tween-80 was 100 mg;the amount of vitamin E was 50 mg;10 ml dichloromethane was used as the solvent. The op-timal pH value of the external phase was 6. 5 with the volume of 10 ml. The ultrasonic time was 8 min. The order of 12-hour cumulative penetration amount was capsaicin liposomes>capsaicin-PBS suspensions>capsaicin ointments. Capsaicin liposomes had the highest 12-hour cumulative penetration amount and showed obvious sustained-release property. Conclusion:Capsaicin liposomes have high en-trapment efficiency, good percutaneous penetration and sustained-release property, and the preparation technology is simple.

Objective To investigate the life quality changes and influencing factors of acute coronary syn -drome after stent implantation .Methods 100 cases with acute coronary syndrome received stent implantation were selected .SF-36 life quality questionnaire was used to survey the patients′life quality before operation and 6 months af-ter operation,and the life quality changes and influence factors were analyzed .Results 6 months after operation,the physical activity ,mental health ,social activities ,physical pain ,physical function ,physical role ,energy and health score of the patients were higher than before operation (P<0.05).According to Logistic regression analysis ,female,type 2 diabetes,underwent percutaneous coronary intervention ,ST-T segment elevation myocardial infarction were the im-portant factors affecting patients′recovery(P<0.05).Conclusion 6 months after stent implantation,the life quality of the patients with acute coronary syndrome were improved obviously;the female and underwent percutaneous coro-nary intervention have positive influence on patients′recovery,while type 2 diabetes,ST-T segment elevation myocar-dial infarction affect patients′recovery in negative way .

Objective: To optimize the flash extraction process of the active ingredients from Lonicera japonica by Box-Behnken design and response surface methodology.Methods: Flash extraction was used.With the concentration of ethanol,solid-liquid ratio and extraction time as the main influencing factors and the overall normalization value of the transfer rates of chorogenic acid and luteoloside as the evaluation index, Box-behnken design was performed to screen out the optimal extraction conditions.The mathematics relationship between the overall normalization value and the independent variables was established by multiple linear regression and binomial fitting, and response surface methodology was used to predict the optimal process conditions.Results: The optimal extraction conditions were determined as follows: the ethathol concentration was 66.92% ,the solid-liquid ratio was 18.82 ,the extraction duration was 1.20 min and extracted only once.Under the above conditions, the extraction rate of chloragenic acid and total luteoloside was 92.87% and 87.55% , respectively.Conclusion: The optimized technology is simple,economic and practical,which can provide new ideas for the rapid extraction and quality control of active ingredients from Lonicera japonica .

Objective To understand the diagnostic value of blink reflex (BR) in central neuropathy by observing the variations of blink reflex in diabetic patients. Methods Electrical pulse was used to stimulate the supraorbital nerve at the supraorbital notch. The responses of bilateral orbicular muscles of the eye were recorded, the variations of the latency and amplitude of R 1 and R 2 recorded on the side of stimulation and of R′ 2 recorded on the contralateral side were measured. Results There was significant prolongation in the latency and lowering of the amplitude of R 1, R 2 and R′ 2 in the diabetic patients compared with the control group. The abnormality rate of BR increased gradually with the prolongation of diabetic duration. Conclusion BR examination might provide an objective index for nervous damage in diabetic patients and it is helpful in early diagnosis of diabetic neuropathy.

Since the children were born,their yin-yang balance in their body began.Children’s yin-yang balance is the yang taken a dominant role in yin-yang balance."Shaoyang for the pivot" is the core of "Shaoyang doctrine"theory.It is very proper to explain the children’s yin-yang balance with the state of continuous transporting and changing by the theory of "Shaoyang for the pivot".Along with "mutual promotion of growth between yin and yang",new yin yang balance continuously replace the old one,constructing children’s basic physical characteristic of "full of vigor and vitality,rapid development;viscera is delicate and organs function is not perfect".Meanwhile it is very proper to explain the pathological characteristics "easy incidence,rapid changing;viscera qi clean and full of spiritualism,easy rehabilitation".So "Shaoyang doctrine" can cover "pure yang doctrine" and "immature yin and yang theory".

Objective To analyze the changes of insulin-like growth factor-1(IGF-1) serum level in diabetic lower extremity angiopathy,explore the role of IGF-1 in the pathogenesis of diabetic vascular complications,and provide basis for prevention and treatment of diabetic angiopathy.Methods We selected 74 type 2 diabetes mellitus(T2DM) patients as case group and 15 normal people as control group.All of the research subjects were measured in height,weight,blood pressure,blood glucose,blood lipid,fast insulin,and urinary albumin excretion rate(UAER).The serum IGF-1 level was measured with ELISA.Arteries of both lower extremities were measured with color Doppler flow image.Results ① The serum IGF-1 level was significantly higher in DM patients than in control group.② The serum IGF-1 level was significantly higher in diabetic patients with lower extremities arterial vasculopathy than diabetic patients without.The results showed a positive correlation between the serum IGF-1 level and severity of lower extremity arterial vasculopathy.③ There was a positive correlation between diabetic duration,UAER,total cholesterol(TC),lower density lipoprotein(LDL) in blood and lower extremity arterial vasculopathy in diabetic patients.Conclusion The serum IGF-1 level can reflect the severity of lower extremity arterial vasculopathy.The serum IGF-1 level may be a reference valuable in diagnosis of diabetic macroangiopathy and a new cure point for diabetic macroangiopathy.