The control system of radiating therapeutic instrument, powerful and reliable, is designed with the new chips of P89LPC669 and MAX669. Its hardware organization, system function and software framework are introduced in this paper.

Subcutaneous injection of 1.0 mg/kg cadmium chloride in 0.8% solution was given to rabbits weighing 1.7~2.5kg 3 times a week for 10 weeks.An equal volume of normal saline was injected to the rabbits of the control group.Blood specimen from the auricle vein and 24-hour-urine of every rabbit were collected once a week.Ten weeks later the renal cortex of the rabbits WES resected,processed routinely and examined under optical microscopy,The level of guanidinoacetic acid (GAA) of the serum and urine was determined with HPLC.It was found that the serum level of GAA increased after 3 weeks of cadmium administration while urinary excretion of GAA increased slightly in the first week and decreased significantly in the second week of cadmium injection and thereafter.

This paper introduces some information of the electronic control system for LGJ-80 freeze dryer, including its system structure, hardware system and software system. The automatic control during overall course, automatic adjustment for degree of vacuum and antijamming are realized in this paper.

Objective To develop a new type of piezoelectric quartz crystal microbalance DNA sensor based on lambda exonuclease to detect Staphylococcus aureus (S. aureus) and optimize the main detection conditions. Methods After the DNA was extracted from the stain of S. aureus and the target fragment was amplified with self-designed universal primers, the PCR products were treated with Lambda exonuclease and visualized by agarose gel electrophoresis with ethidium bromide followed by a gel documentation system analysis. Then the products with or without exonuclease digestion were added into our piezoelectric quartz crystal microbalance DNA sensor for hybridization. The temperature and time of hybridization were optimized respectively. The specificity and sensitivity of our detection system were evaluated. Results The optimal temperature for hybridization was 35℃ and the optimal time was 60 min. The sensitivity of the lambda exonuclease based DNA sensor was better than that of common DNA sensor. The lowest detection limit of this new type quartz crystal microbalance system was 1.0?104 CFU/ml. Conclusion The lambda exonuclease based quartz crystal microbalance system we designed has the advantages of high efficiency in hybridization, easy to operate, and good response performance. Thus it can be applied to detect S. aureus infections.

OBJECTIVE To analyze the reasons of nosocomial infection in laboratory departments, and then advance corresponding measures to overcome them. METHODS The current situations in laboratory department between domestic large scale hospitals and overseas hospitals were compared, especially paying attentions to those parts involving in management system and precautionary measures. RESULTS There were a lot of shortcomings existed in the supervision of nosocomial infections in laboratory departments; many measures should be taken to increase the management level. CONCLUSIONS To reform and improve the system of nosocomial infection control and prevention, and establish an effective and systematic alerting and prevention system will benefit all kinds of the hospitals.

To measure the rotating speed and temperature of the centrifuge, an equipment and method are developed in this paper. They prove to meet the requirements of measuring. The equipment and method can be used to measure the rotating speed or temperature of the operating centrifuge easily and accurately. That is also the main excellence of them.

OBJECTIVE To study the main bacteria and their drug-resistance of postoperative infections after liver transplantation.METHODS The distribution and drug-resistance profile of 156 strains of bacterial isolates from(various) specimens in 68 liver transplantation inpatients were retrospectively analyzed last year.RESULTS The(incidence) of infections after liver transplantation was 67.6 %.The major bacteria were Klebsilla pneumoniae((17.3%)),Staphylococcus aureus(14.1%),Candida albicans(11.0%),and Pseudomonas aeruginosa((11.0%)).The bacteria had the character of multidrug-resistance and high drug-resistance.The most effective(antibiotics) to Gram-negatives and Gram-positives were still the carbopenems and glycopeptide.CONCLUSIONS(Infection) is a major complication after liver transplantation.Prevention,early diagnosis and treatment of the(infection) are very important.

OBJECTIVE To develop the strategies for preventing nosocomial infection in the department of(laboratory).METHODS The current situation in nosocomial infection management in department of laboratory was analyzed.RESULTS There were some problems in training knowledge of nosocomial infection,preparing(equipment) and supplies for prevention,implementing prevention measures,and cultivating good work habit.(CONCLUSIONS) It is very important to strengthen nosocomial infection management for preventing efficiently(nosocomial) infection in the department of laboratory.

Objective To analyze the clinical distribution ,antimicbial resistance and Staphylococcal cassette chromosome mec (SCCmec) genotype characteristics of 346 methicillirrresistant Staphylococcus aureus (MRSA) clinical isolates in the hospital . Methods A total of 784 strains of Staphylococcus aureus isolated from January 2014 to January 2015 in the hospital ,MRSA identi-fication and the SCCmec genotype was conducted by PCR assay .Results 346 strains of MRSA (44 .13% ) were isolated from 784 strains of Staphylococcus aureus ,the detection rate of MRSA from sputum accounted for 43 .06% ,the secretion accounted for 48 .55% .MRSA was resistant to penicillin ,levofloxacin and erythromycin ,sensitive to vancomycin ,linezolid and teicoplanin .SCC-mec genotyping result showed that SCCmecⅡ was identified in 130 ,SCCmecⅢ in 196 ,SCCmecⅣ in 11 ,SCCmecⅤ in 9 .Conclusion SCCmecⅢ is the main genotypes of MRSA from our Hospital ,all of the MRSA strains are multi-resistant to tested antibiotics , but sensitive to vancomycin and teicoplanin .

Objective To study the relationship of concentraions of trace elements and the SOD, GSH-Ps activities in plasma following severe burn injury, and provide witness for clinical treatment of trace elements disorder in burned patients. Methods The plasma and urine from 67 severely burned patients in our hospital were collected on day 2, 4, 6, 8, 10, 15, 20, 30 after hospitalization. Trace element levels were determined by atomic absorption spectrophotometry. The vital force of SOD and GSH-Px were measured in plasma with the kits provided by Nanjing Jiancheng bioengineering research institute. Results The ion concentration of Cu in serum got higher and that of Zn got lower after burn, but that of Mn, Se was of no significance. The concentration of Cu, Zn, Mn, Se in urine were increased. The enzymatic activities of T-SOD and Cu, Zn-SOD as well as GSH-Px decreased from the early period to the metaphase. Conclusion For a great deal of oxygen free radicals released by tissues, SOD and GSH-Px were consumed and their concentration decreased in early stage after burn. Due to the trace elements released into blood by destroyed tissues and their increased excretion from urine, tissues could not generate adequate antioxidase, resulting in weak ability against inflammation and poor tissue repair. Our result provides a theoretical clue for the replenishment of trace elements for severely burned patients in early and middle stage and offer a clue to pathology and etiological treatment of burn.