A fluorescence quenching method was established for the determination of H2 S in intestinal perfusate by optical fiber sensing technology combined with micro sequential injection lab_on_valve (μSIA_lov). In the experiment, 100 μL of 0. 1 mol/L NaOH was used as the carrier, and 50 μL of 5. 0×10-5 mol/L fluorescein mercury and 50 μL of sample were selected for the determination. The detection flow rate at the flowcell was 25 μL/s. According to H2 S quenching fluorescence at 521 nm, the concentration of H2 S in the sample was determined. The detected concentration range of H2S was 5. 0×10-6-8. 0×10-5 mol/L, and the detection limit was 5. 4×10-7 mol/L. Detection result of H2S in intestinal perfusion was 3. 8×10-5 mol/L with 3. 1% RSD (n=3). This method can be used for the determination of H2S effectively in the samples, which lay the foundation for real_time online measurement of H2 S in biological samples.

Objective:To explore the mechanism of traditional Chinese medicine compound (Yang Yin Kang Du powder) in radioprotection.To study the relationship between T lymhocytes and antioxidation of mice radiated with X ray.Methods:T lymphocyte subset of in peripheral blood mice was determined by indirect immunofluorescence and flow cytometer.To T AOC,Cat,SOD and MDA were anoly zedby chemical colorimetry.Results:Compared with the normal group,the percentage of CD4 and CD8 in two irradiated groups declined significantly( P

Objective: To investigate the influence of the closed re-infusion of intestinal juice and EN on the treatment of intestinal fistula.Methods: The study group was composed of 21 patients of intestinal fistula from October 2004 to December 2006.The fistulae was in the 50 cm of the proximate jejunum and caused by trauma and operatin.The leak amount of intestinal juice was more than 1 000 mL.The protein level in serum and the absorption rates of the glucose,fat and nitrogen after the reinfusion of the intestinal juice were measured.Resutls: The protein level in serum and the absorption rates of the glusose,fat and nitrogen was increased after the re-infusion of the intestinal juice.Conclusion: The absorption of the glucose,fat and nitrogen in EN and the protein level in serum can be increased after the closed re-infusion of the intestinal juice.

[Objective] To investigate the effects of Yangyin Kangdu Powder (YKP) on peripheral blood T-lymphocyte subsets levels in X-ray-irradiated mice. [Methods] Forty-five mice were randomized into normal control, model and YKP groups. Except the normal group, the rats in model and YKP groups were irradiated with 6Gy X-ray to establish models with acute radiation-induced injury. Normal saline was given to the model group while YKP in the dosage of 10 g?kg-1?d-1 was orally administered to YKP group for one week. The changes of CD4 and CD8 T-lymphocyte subsets levels were detected by flow cytometry. [Results] Plasma levels of CD4 and CD8 and the ratio of CD4/CD8 decreased in the model group, the difference being significant as compared with the normal control group (P

Objective To investigate the key points for management of subclinical and agonal types of penetrating cardiac trauma (PCT).Methods A retrospective analysis was conducted on clinical data of 135 PCT cases treated from January 2005 to March 2012.The cases were divided into subclinical type,clinical type (cardiac tamponade or hemorrhagic shock types) and agonal type.Managements of the two extreme types including subclinical type and agonal type were studied in groups.Results (1) Thirty cases of subclinical type failed to have timely diagnosis and treatment due to the withdrawal from inhospital observation,which resulted in 22 deaths.Eleven cases of subclinical type had timely diagnosis through in-hospital observation or cardiac exploration,but three cases died in operating room thoracotomy (ORT).There were 27 cases of agonal type,but 15 died in ORT and two died in emergency room thoracotomy (ERT).Of 67 cases of clinical type,seven died in ORT.(2) A total of 86 cases survived ORT or ERT after timely diagnosis or diagnosis through in-hospital observation plus cardiac exploration.In the meantime,sound recovery was observed in 3-24 months of follow-up.Conclusion Success rate in treatment of PCT can be enhanced by close observation and timely cardiac exploration for subclinical type PCT and by timely ERT and bleeding control for agonal type PCT.

Objective Discuss clinical type and treatment of postoperative digestive tract fistula and associate complications of esophageal or cardiac cancer.Methods Analized clinical manifestations and treatment retrospectively of 53 cases of digestive tract fistula after operation of esophageal or cardiac cancer from January,2010 to December,2012.Results All the 10 undergoing surgery are cured by reoperation.2 died of mediastinal infection and sepsis.4 died of respiratory failier,malnutrition,left and right bronchal fistula,respectively.Conclusion There are 4 types of fistula:Ⅰ type of sepsis,Ⅱ type of respiratory failure,Ⅲ type of thoracic infection,Ⅳ type of neck inection.Reoperation within 24 hours is the key of suscessful repairment of intrathoracic anastmotic leakage.Bronchal fistula or respiratory failure caused by multiple thoracic encapsulated effusion is also the indication of surgery.Complete lung reexpansion and complete drainage is the key.Mini-invasive VATS is the method of exact drainage.

Objective To summarize the causes of patients with fever of unknown origin (FUO) and to analyze the relationship between infectious diseases and FUO,in order to provide envidence for experiential therapy.Methods Clinical data of 374 FUO inpatients at He'nan Provincial People's Hospital from June 1,2009 to May 31,2013,including gender,age,diagnosis and department were analyzed retrospectively.Results Three hundred and twenty-seven cases among the overall 374 FUO patients (87.4％) were eventually etiological diagnosed based on supplementary examinations or diagnostic treatment.As for the causes of fever,209 were infection,accounting for 55.9％,among which 78 cases (20.9％) were diagnosed with tuberculosis,23 cases (6.1％) brucellic diseases,19 cases (5.1％) rickettsia infection.Meanwhile,the noninfectious diseases,such as connective tissue diseases (47,12.6％),hematonosis (37,9.9％) as well as the solid tumors (13,3.5％) also constituted considerable shares of the causes for FUO.However,the causes of 47 cases (12.6％) were not identified before discharge.Conclusions Infectious diseases are the main cause of FUO,in which tuberculosis accounts for the majority.Brucellosis and rickettsia infection also account for a considerable proportion.The causes of most FUO cases could be identified through detailed analysis of clinical data and supplemental examinations.

Objective To study on the relationship between the TCM Syndromes and neurological impairment of stroke patients.Methods The clinical data of ischemic stroke which based on the clinical information collecting platform was extracted to test whether there was a linear relationship between the Criterion for Diagnosis and Differentiation of Syndromes of Stroke (CDDSS) and National Institutes of Health Stroke Scale (NIHSS) by means of SPSS 12.0 and genetic algorithm.If there was not a linear relationship between CDDSS and NIHSS,the nonlinear relationship was to be tested by means of BP nerve net,LVQ nerve net,Fuzzy ARTMAP nerve net and Support Vector Machine (SVM).Results There was no linear relationship existed between CDDSS and NIHSS with the application of SPSS 12.0 and genetic algorithm.There existed a complicated nonlinear relationship between CDDSS and NIHSS by using BP nerve net,LVQ nerve net,Fuzzy ARTMAP nerve net and Support Vector Machine (SVM).Conclusion Between CDDSS and NIHSS,there isn't a linear relationship,but a complicated nonlinear relationship.

Objective:To analyze the prevalence of syphilis infection and influence factors of syphilis infection among the male STD attendants in Songyuan City of Jilin Province, and to provide theoretical basis for the development and implementation of syphilis intervention policy.Methods:A questionnaire survey and serological tests were conducted among the men who went to STD clinic for treatment in the ZhanDong Dermatology Specialist Hospital or Qianguo County Hospital in Songyuan City from 2011 to 2015.Chi-square test was performed to compare the prevalences of syphilis infection between different groups.Multivariable Logistic regression model was used to find the independent factors of syphilis infection among the male STD attendants.Results:Of all 2 000 male STD attendants who involved in the study,the mean age was (34.50±9.03)years old.218 persons (10.90%)of them had sexual behavior with female sex workers in the last three months,433 persons (21.65%)of them had temporary sexual behavior in the last three months,42 persons (2.10%)of them had homosexual behavior in the last three months.Of the respondents,238 persons (11.90%)had a previous diagnosis of sexually transmitted diseases,86 persons of them had gonorrhea (36.13%),43 persons had syphilis (18.07%),15 persons had genital tract Chlamydia (6.30%),55 persons had genital herpes (23.11%),40 persons had genital herpes (16.81%),and 1 person had combined infection of gonorrhea and syphilis. The prevalence of syphilis of 2 000 male STD attendants was 6.4%.From 2011 to 2015,there was a decrease in the prevalences of syphilis (χ2 =44.25,P <0.001).In the last 3 months, the risk of the male STD attendants who had commercial sex with prostitute,temporary sex behavior,homosexual behavior and a history of STD infection to be infected with syphilis in the past were 3.75 times (OR= 3.75,95% CI:2.46 - 5.71),2.31 times (OR = 2.31,95% CI: 1.56 - 3.41),2.97 times (OR=2.97,95%CI:1.33-6.64)and 1.69 times (OR=1.69,95%CI:1.07-2.67)than those who did not have those behaviors,respectively. Conclusion:There is a significantly decreasing tendency in the prevalence of syphilis among the male STD attendants in Songyuan City in the past 5 years.The high risk sexual behaviors are the main influence factors of syphilis infection among them.

To observe the influence of the placental apoptosis on the expression of Bax,Bcl-2, Fas, FasLand TNF-α during the second trimester of pregnancy, mice of experimental group were intraperitoneal injected with 100 purified Toxoplasma gondii tachyzoites added in 0.2mL of PBS, while those of the control group were injected with 0.2 mL of sterile PBS (0.01 mol/L, pH 7.4) in the 8-th day of pregnancy. During the 12, 14, 16 and 18-th days of pregnancy, 5 mice both in experimental and control group were randomly killed and the expression levels of the apoptosis-related proteins Bax, Bcl-2, Fas, FasL and TNF-α in the placental tissues were determined by means of immunohistochemical methods. It was showed that the apoptosis-related protein expressed both in villus and decidua of the placenta, most of which were expressed in syneytiotrophoblast (ST). The positive cells with expression of Bax, Fas, FasL and TNF-α increased along with the increase of the pregnant days in both the experimental group and the control group, and the positive cells with expression of Bcl-2 decreased along with the increase of the pregnant days. It was also demonstrated that the positive cells with expression of Bax, Fas, FasL and TNF-α of the experimental group showed a higher percentage of expression than that of the control group on the same pregnant days, but the positive cells with Bcl-2 expression of the experimental group were fewer than that of the control group. It is concluded that the expression of apoptosis-related protein Bax, Bcl-2, Fas, FasL and TNF-α in the placenta were altered when the pregnant mice were infected with Toxoplasma gondii during the second trimester, which may induce the apoptosis through the endogenic and ectogenic pathway.