OBJECTIVE:To establish a method for separation and determination of ethanol,dichloromethane and N,N-dimethylformamide in pidotimod tablets.METHODS:The residual organic solvents were quantitatively determined by GC on DB-624 capillary column with FID detector,using nitrogen as the carries gas.Temperature programming was carried out in which the column temperature was initially maintained at 40 ℃ for 5 minutes,then increased at a rate of 50 ℃ per minute to 200 ℃,and maintained at 200 ℃ for at least 10 minutes.The temperature at injection mouth was maintained at 160 ℃;the detector temperature was maintained at 280 ℃.The split ratio was 5∶1.RESULTS:3 residual organic solvents were completely separated and their determination was free of inference from hidden foreign substances.The linear ranges of the ethanol,dichloromethane and N,N-dimethylformamide were 10.036～802.88 (r=0.999 9),26.44～105.76 (r=0.999 6),and 1.76～140.8 (r=0.999 5) ?g?mL-1,respectively,and their average recovery rates were 102.4%,99.0%,and 99.2%,respectively,with RSD at 1.99%,3.34%,and 0.55%,respectively.CONCLUSION:The method is sensitive,accurate and rapid,and it is suitable for determination of residual organic solvents in pidotimod tablets.

The Holy Bible, as the root of Western civilization, has imposed great influence in the fields far beyond religion. In this thesis, the author intended to reveal the medical implication in the Holy Bible and its relevance to the modern medical science by exploring the biblical medical information and comparing it with the current medical theory and practice. The conclusion of the exploration is surprising yet inspiring: the Holy Bible, as an ancient religious book, contains rich medical information around themes such as sexual relations, dietary guidelines, hygiene, etc., which is not at odds, but in harmony with the modern medicine.

Objective To analysis of treadmill exercise test Duke score(DTS) in patients with coronary heart disease than the evaluation functions of heart operation perioperative cardiac events.Methods One hundred and eighty-four patients with coronary heart disease,45 ～ 75 years of medium-sized non cardiac operation,were chose from May,2010 to May,2011 in our hospital,Cardiac ultrasound,treadmill exercise test were taken before operation,preoperative examination department of internal medicine disease history and physical examinations.According to the Duke score,they were divided into DTS in low risk group (5 ≤ Duke ≤ 15,n =124),medium risk group DTS(DTS:(-10≤Duke≤5,n =60),the exclusions of patients with high-risk DTS group,and the high-risk group of patients were excluded.Comparative analysis of operations,the main type of Department of internal medicine disease history,intraoperative and postoperative major cardiovascular complications.Results In the two groups of operation type and the type of anesthesia and cardiovascular disease,there is no significant difference (P ＞ 0.05) ; in DTS low risk group the age (59.2 ± 4.1) years,preoperative cardiac dysfunction were 2 cases,left ventricular ejection fraction ＜ 0.50 were 2 case (1.6％),diabetes history 12 case(9.6％),with angina pectoris symptoms of 51 case(41.1％),ECG ischemic changes in 55 (44.3 ％),in the medium DTS risk group,the age(65.2 ± 2.6)years,preoperative cardiac dysfunction were 8 cases(13.3％),left ventricular ejection fraction of ＜ 0.50 was 5 case (8.3％),diabetes history 23 case (38.3 ％),angina pectoris and 60 case (100％),ECG ischemic changes in 40 case (66.7 ％) (t =2.98,P =0.042,x2 values were 4.93,3.84,4.67,5.24,3.58,P ＜0.05).The low risk group of patients with arrhythmia,hypertension incidence rate were 6.5％ (8/124) and 22.5％ (28/124),medium risk group of patients with arrhythmia,hypertension incidence rate were 11.6％ (7/60) and 18.3％ (11/60),compared with DTS in low risk group,DTS medium risk group of arrhythmia and hypertension odds ratio(Odds Ratio,OR) and 95％ confidence intervals were 1.7 (0.8-3.3),0.8 (0.4-1.4),the P values were 0.062,0.074,has no significant difference.Controlling for age and sex DTS in low risk group postoperative myocardial infarction,cardiogenic pulmonary edema rate were 0.8％ (1/124) and 2.4％ (3/124),medium risk group after DTS myocardial infarction,cardiogenic pulmonary edema rate were 10％ (6/60) and 11.7％ (7/60),compared with DTS in low risk group,DTS medium risk group after myocardial infarction and heart pulmonary edema ratios (Odds Ratio,OR) and 95％ confidence intervals were 19.3 (5.6-66.2),5.7 (2.5-12.9),the P values were 0.002,0.003,had significant diflerence.Conclusion DTS medium risk group patients undergoing non cardiac operation preoperative heart failure,diabetes,angina symptoms,peri operation period of cardiac event rate is high and heavy.

Objective To investigate the clinical characteristics of vaccine-associated immune thrombocytopenia(VA-ITP)based on the US Vaccine Adverse Event Reporting System(VAERS), and to provide a reference for vaccine safety monitoring, vaccination prevention, and clinical diagnosis and treatment.Methods Data from VAERS on all reported cases of immune thrombocytopenia(ITP) after vaccination since the establishment of the database in 1990 to the data lock point of 7th November 2023 were analyzed retrospectively for information on such as sex, age, vaccine type, vaccine dose, and occurrence time of ITP.Results Among the 877 cases of VA-ITP, there were 868 cases of effective statistical vaccine types, involving 43 vaccine types. The top 10 vaccine types were COVID-19 vaccine(434 cases, 50%), MMR vaccine(82 cases, 9. 4%),HEPA vaccine(48 cases, 5. 5%), FLU3 vaccine(41 cases, 4. 7%), FLU4 vaccine(32 cases, 3. 7%), HPV4 vaccine(30 cases,3. 5%), DTAP vaccine(29 cases, 3. 3%), MMRV vaccine(32 cases, 3. 7%), VARZOS vaccine(17 cases, 2. 0%) and HPV9vaccine(13 cases, 1. 5%). Among the 877 reported cases, 843 cases with definite sex were counted, including 374 males and469 females, with a male-to-female ratio of 1∶1. 25. The 377 cases of patients were under 18 years of age, accounting for about 44. 7% of the total, and there was significant difference in the composition ratio of sexes among patients with VA-ITP at different ages(χ~2= 10. 029, P < 0. 05). The incidence of ITP within 8 days after vaccination, with a total of 336 cases, was39.9%, and there was no significant difference in the occurrence time composition ratio of ITP between different sexes(χ~2=3. 296, P > 0. 05). The 54. 9% of ITP occurred after the first dose of vaccine, and there was no significant difference in the composition ratio of different vaccination doses between male and female recipients(χ~2= 5. 323, P > 0. 05), but there was significant difference in the composition ratio of vaccination doses among different age groups(χ~2= 30. 591, P < 0. 01).Conclusion By analyzing the summary data from VAERS database, the adverse reaction of VA-ITP was fully recognized.After clinical vaccination, attention should be focused on the platelet status of minors and the elderly, as well as those who were vaccinated within 8 days, and the continuous vaccine safety monitoring should be carried out, which is helpful to reduce the missed diagnosis of vaccine adverse events(AE) and improve the efficiency of clinical diagnosis and treatment.

Radiotherapy is one of the most important treatments for glioma; however its therapeutic effect is unsatisfying. Timing, accurate target outlining, a total dose higher than 60Gy, conformal modulating radiotherapy and combined chemotherapy and targeted therapy are important factors in improving the outcome of radiotherapy.

Objective To find out the prevalence of iodine deficiency disorders (IDD) and population iodine nutritional status,and to evaluate the intervention effect of iodized salt during the Twelfth Five-Year Plan period,and to provide a scientific basis for adjusting the intervention programs.Methods One town was selected respectively from the east,the west,the south,the north and the central of the entire city,4 villages were selected from each town,15 residents were selected to determine iodide content by quantitative detection;1 primary school was selected respectively from those 5 chosen towns,40 subjects aged from 8 to 10 years old (half male and half female) in each school were selected to collect urine samples for determination of urinary iodine,and to detect the volume of thyroid by palpation;20 pregnant women were chosen respectively from those 5 towns to detect their urinary iodine content and to determine iodine content of their salt samples,10 pregnant women were selected to investigate their healthy knowledge.Results A total of 300 samples of edible salt were tested,the median of salt iodine was 21.2 mg/kg,coverage rate of iodized salt was 97.7％ (293/300),the consumption rate of eligible iodized salt at household level was 90.3％ (271/300);the median of urinary iodine level was 232.4 μg/L in 200 8-10 years old chindren;232.9 μg/L in 100 pregnant women;the prevalence rate of goiter in children aged 8-10 was 3.0％ (6/200);the healthy knowledge rate of pregnant women was 82.0％ (41/50).Conclusions During the Twelfth Five-Year Plan period,Zaoyang has achieved the stage of elimination of IDD,but the situation of iodine nutritionrich children and the iodine nutrition-lack pregnant women are still exist.Therefore,we still need to strengthen the salt market management and insist to spread the knowledge of IDD,to eliminate the non-iodized salt from the market,and to ensure all people in appropriate iodine nutrition.

Objective To investigate the clinical effect ofFuzheng-Shugan decoction on the treatment of patients with the treatment nodule.Methods A total of 80 patients with thyroid nodule from Jan.2014 to Dec.2015, divided into two groups. The efficacy, thyroid stimulating hormone, high risk factors, the maximum diameter and volume of thyroid nodules were observed.Results The effective rate of observation group was significantly higher than of the control group (90.00%vs.27.50%,χ2=32.237,P=0.000). The shortness of breath (1.62 ± 0.86vs. 4.35 ± 1.13,t=12.159), foreign body sensation (2.53 ± 0.62vs. 4.23 ± 0.56,t=12.289), irritability (1.56 ± 0.53vs. 3.52 ± 1.25,t=9.130), sweating (1.85 ± 0.62vs.4.23 ± 0.56,t=18.017), tongue (1.58 ± 0.93vs. 3.71 ± 1.25,t=8.646) and neck pain (0.82 ± 0.35vs. 3.92 ± 0.65,t=26.558) in the obversation group were significantly lower than those in the control group (P<0.05). The thyroid stimulating hormone levels in the observation group (1.05 ± 0.21 mIU/Lvs. 2.16 ± 0.71 mIU/L,t=9.482), were significantly lower (P<0.05). The maximum diameter (8.52 ± 4.72 mmvs. 12.27 ± 5.69 mm,t=3.208) nodule volume (0.26 ± 0.71 cm3vs. 0.96 ± 1.02 cm3,t=3.562) and high risk factors (2.73 ± 1.45vs. 4.05 ± 1.36,t=4.199) in the observation group were significantly lower than those in the control group (P<0.05).Conclusion TheFuzheng-Shugan decoction can improve TCM syndrome, reduce the risk factors, and effectively reduce the nodule maximum diameter and volume.

EBP50(ERM-binding phosphoprotein-50),a multifunctional adapter protein with 358 amino acids and two PDZ domains, regulates cell growth and migration. Lines of evidences indicate that it is a potential cancer suppressor protein. Loss of heterozygosity (LOH) and intragenic mutation of the ebp50 gene have been found in both primary breast tumors and breast cancer cell lines. EBP50 suppresses the breast cancer cell proliferation via its interaction with many tumor suppressor protein including PTEN, SYK, MERLIN, etc. Here the molecular structure of EBP50, signal pathway regulated by EBP50, and the relationship between breast cancer development and EBP50 are discussed.

The application of 18 F-FDG PET-CT in the diagnosis and treatment of nasopharyngeal carcinoma includes early diagnosis,clinical staging,contouring target volume,predicting the sensitivity of tumors to radiotherapy and effect evaluation,and monitoring the residual and recurrence.However,18 F-FDG uptake of tumor cells is under the influence of various factors,so false positive and false negative are unavoidable.Therefore it is need to develop more specific tracer agent in order to improve the accuracy of diagnosis and treatment,as well as to carry out multicenter clinical trial to identify the correlation of standardized uptake value (SUV)and radiation target volume.

During radiation for head and neck cancers,part of parotids are contained in planning target volume(PTV),which lead to parotids receiving radiation as the same as PTV.Radiation for head and neck cancers will influence the parotids.In the period of radiation,many factors will influence parotids,such as age,primary parotid volume,V10-40,weight and mean radiation dose et al.These factors will result in that the parotid volume reduces nearly 50％ and moves toward the middle line of the body.As a result,the real dose of the parotid receiving is higher than prescription dose.The excretion function of the parotid will be severely damaged after radiation,which will lead to xerostomia and influence the quality of patients life.