Objective To study the effect of AG490 on the invasion and metastasis of gallbladder SGC -996 cells regulated by JAK/STAT3,and discuss the related mechanism.Methods Cell viability treated with different concentrations of AG490(50,100,200μmol/L)was detected by MTT method.The ability of invasion and metastasis of gallbladder cells was evaluated by Transwell membrane count.The SGC -996 cell apoptosis was detected by flow cytometry.The protein expression of JAK/STAT3 pathway was detected by Western blot.Results The viability inhi-bition rates of different concentrations of AG490 for SGC -996 cells were (17.49 ±3.41)%,(38.66 ±4.57)%, (79.15 ±6.29)% respectively,and with the increasing of concentration,cell viability decreased obviously.Compared with the control group[(1.39 ±0.21)%],the differences were statistically significant(t =8.162,14.111,21.401, all P <0.01 ).The transfer ability inhibition rate of different concentrations of AG490 for SGC -996 cell were (23.18 ±4.53)%,(51.75 ±6.46)%,(81.32 ±7.13)% respectively,and with the increasing of concentration of AG490,the inhibition rate of invasion and metastasis enhanced.Compared with the control group [(1.46 ± 0.42)%],the differences were statistically significant(t =8.269,13.455,19.366,all P <0.01).The apoptosis rate for SGC -996 cells of different concentrations of AG490 were (13.34 ±4.33)%,(28.16 ±6.23)%,(53.61 ± 8.74)% respectively,and cell apoptosis increased with the increasing of concentration.Compared with control group [(0.97 ±0.52)%],the differences were statistically significant(t =4.913,7.533,10.414,all P <0.01).Different concentrations of AG490 can reduce expression of ZFX,STAT3 and Smad1 protein of JAK/STAT3 pathway of SGC -996 cells,compared with the control group,the differences were statistically significant(tZFX =2.154,3.041,4.185, tSTAT3 =7.348,14.892,17.774 and tSmad1 =3.474,5.241,7.718,all P <0.05).Conclusion AG490 can inhibit the invasion and metastasis of gallbladder SGC -996 cells,and the effect depends on dosage.Its mechanism may be relat-ed to the reduction of cell apoptosis and the protein expression of JAK/STAT3 pathway.

Objective To observe the effect of somatostatin combined with transcatheter arterial infusion chemotherapy in the treatment of malignant intestinal obstruction.Methods 60 patients with malignant intestinal obstruction were enrolled in our hospital from January 2013 to June 2015.The rats were randomly divided into 4 groups: the conventional group(n=15),the growth inhibition group(n=15),the low dose intra-arterial infusion chemotherapy group(n=15)and somatostatin plus low-dose intra-arterial infusion chemotherapy group(n=15).The clinical symptom remission time,duration of clinical symptom remission and survival time were compared between the four groups.Results The study showed that somatostatin group of gastrointestinal decompression and symptom remission time were significantly better than the conventional group.Especially the symptom remission time is significantly faster than the conventional group and chemotherapy group(P< 0.05); the duration of chemotherapy group and somatostatin+artery perfusion chemotherapy group in survival time and relieve symptoms,were significantly better than the conventional group and somatostatin group(P< 0.05).Conclusion Somatostatin is beneficial to reduce the amount of gastrointestinal decompression in the treatment of malignant intestinal obstruction.Celiac artery infusion chemotherapy can help to improve the maintenance time and prolong the survival time of patients with malignant obstruction.The combination of somatostatin and celiac artery infusion chemotherapy is beneficial to relieve the symptoms and prolong the time interval,and improve the survival time of patients.