Neovascularization is the main cause of blindness, such as diabetic retinopathy, retinopathy of prematurity and age - related macular degeneration. Vascular endothelial growth factor( VEGF) plays an important role in the formation of angiogenesis, and is considered to be the most potent angiogenic growth factor. Placental Growth Factor(PlGF) is one of the VEGF family, which play a crucial role in endothelial cell proliferation and migration, angiogenesis, and immune - mediated inflammation. Meanwhile, PlGF is specifically expressed in pathological angiogenesis, but not in normal blood vessels. In recent years, there has been increasing attention to PlGF, therefore this article reviews the role of PlGF in neovascular ocular diseases.

BACKGROUND: Pulsed electromagnetic field attracts much attention due to its less trauma, infection, side effect, and simple application to promote bone fracture healing.OBJECTIVE: To review the effect of pulsed electromagnetic fields on bone fracture healing by mechanism, animal experiments, and clinical application so as to provide theoretical evidences for promoting bone fracture healing. METHODS: This study was retrieved by the first author with the key words of "PEMF and fracture" in both English (1989-2009)and Chinese (1981-2009) from Pubmed database (http://www.ncbi.nlm.gov/Pubmed) and Vip database (www.cqvip.com). Articles which were related with the effect of pulsed electromagnetic fields or electromagnetic field on bone fracture healing were included.The duplicated articles were excluded. The retrieval articles included reviews, animal experiments, and clinical experiments.Among 62 articles, 32 were in English and 30 in Chinese. Following the primary retrieval, 9 articles were excluded due to unrelated researching aim, 6 due to duplication, and 7 due to reviews. A total of 40 articles, 16 in Chinese and 24 in English, wereincluded in the final analysis, including animal and cytological experiments (n=31), and clinical studies (n=9).RESULTS AND CONCLUSION: Pulsed electromagnetic field characterized by security, environmental protection, non-trauma,non-infection, sample processing, wide applicability, and less complications for treating bone fracture healing. Clinical experiments and clinical application have proved that pulsed electromagnetic field was an effective method to promote bone fracture healing, characterizing by low frequency and intensity. The mechanism of pulsed electromagnetic field for promoting bone fracture healing involved in physics and medicine. With the research of electromagnetism, organic complexity, and ethics, the parameters of pulsed electromagnetic field need to be further studied.

Objective Sterol regulatory element-binding proteins(SREBPs) are a family of nuclear transcription factors that regulate lipid metabolism in eukaryotic cells.They are over expressedin many malignant tumors,and closely related to tumor initiation and progression.We briefly summarize the research advancement of SREBPs in gynecological tumors.

Objective To observe the effect of dexmedetomidine on patients′ inflammation during CPB and protective effect on kidney and liver. Methods 60 cases undergoing cardiac valve replacement under CPB were randomly divided into NS group and Dex group. Blood samples were taken before induction , before ascending aorta blocked, end of CPB, 24, 48 and 72 hours after operation. The serum level of HMGB-1, TNF-α, IL-6, BUN, Cr and ALT are tested. Blood WBC and N% are also counted. Results WBC, N% and HMGB-1, TNF-α, IL-6, BUN, Cr in Dex group significantly decreased at time point T2 ~ T6 (P < 0.05) compared with NS group. But ALT in Dex group only decreased at time point T 2 and T5 compared with NS group (P < 0.05). Conclusion Dexmedetomidine can significantly decrease inflammatory factor during CPB and improve renal function after surgery.

Objective To study the growth and development as well as reproductive capacity of CL Long Evans rats. Methods Eighty CL Long Evans rats(80 days old)were bred in a barrier system. A monogamous mating system is used to reproduce 5 litters. Their main indexes of growth and reproduction were determined. The body weight gain of 60 second litter rats were analyzed from day 20 to 100. Results The number and weight of the fetus, the weaning time weight per cote and per litter in the second litter were the highest, and the fifth litter was the lowest. The growth and development curve was the same as that announced by Taconic. Conclusion Long Evans rats have been confirmed suitable for continual reproduction for 4 litters and the second litter is the best one for mating.

The experiment was done to explore the effect of sodium Tanshinone Ⅱ_A, sulfonat (STS) on myocardial reperfusion injury. Myocardial ischemia was induced with the occlusion of left anterior descending coronary artery lasting 30 minutes, and postischemic reperfusion persisted 30 minutes also. Fifty rabbits were randomly divided in to five groups: operative control group (group Ⅰ, n=10), myocardial ischemia group (group Ⅱ, n=10), myocardial ischemia and reperfusion group (group Ⅲ, n=10), receiving intravenous STS 5mg?kg~(_1) 5 mins before the reperfusion (group Ⅳ, n=10) and receiving intravenous STS 5mg?kg~(_1) 10 mins before the ischemia and 5 mins before the reperfusion respectively (group Ⅴ, n=10). As compared with those of group Ⅰ, the amount of myocardial malondialdehyde (MDA) increased significantly in group Ⅱ and Ⅲ (P0.05); the activity of myocardial superoxide dismutase (SOD) decreased in group Ⅲ(P0.05); the serum creatine kinase (CK) activity rose markedly in group Ⅱ, Ⅲ and Ⅳ (P0.05). In comparison with those of group Ⅲ, the amount of MDA reduced and activity of SOD increased in group Ⅳ and Ⅴ (P

Objective To explore the change of alveolar epithelial liquid clearance capacity in lung edema following acute lung injury induced by oleic acid.Methods Forty-eight Wistar rats were randomly divided into 6 groups: the control(C), injury(I), amiloride(A), ouabain(O),amiloride plus ouabain(AO), and terbutaline(T) groups. Acute lung injury was induced with intravenous oleic acid 0.25 mlkg -1. 24h after injury, 5% albumin solution (5 ml?kg -1) was delivered into both lungs via the trachea in C and I groups. In A, O, AO and T groups, amiloride (2?10 -3 mol/L),ouabain (5?10 -4 mol/L), amiloride (2?10 -3mol/L) and ouabain (5?10 -4 mol/L)mixture and terbutaline(10 -4 mol/L),added respectively to the albumine solution,at 5ml.kg -1 were administered intratracheally to both lungs separately. One hour later, the alveolar liquid clearance rate(ALC), total lung water content(TLW), extravascular lung water content(EVLW) and arterial blood gases were measured.Results As compared with those in C group, severe hypoxemia, hypercapnia and acidosis appeared, ALC was reduced by 49.2% ,TLW and EVLW markedly increased in I group(P

Articular cartilage repair is limited. Current treatments for cartilage defect are less satisfactory, and rarely restore full function or return the tissue to its native normal state. The rise of tissue engineering holds great promise for the generation of functional cartilage tissue substitutes. The history of cartilage tissue engineering and highlights the applications and advantages of various kinds of scaffolds in cartilage tissue engineering, such as native scaffolds, synthesis scaffolds, composite scaffolds and nanometer scaffolds had been introduced. But native scaffolds have weak strength and immunogenicity insufficiency, synthesis scaffolds degrade quickly, whose degrading products have cytotoxicity,which need further improvement. The application of superficial decoration overcomes the disadvantage of some scaffolds to an extend. Composite scaffolds possess the advantages of several scaffolds, it points out the direction of future scaffolds research. The development of Nanometer technique endows newly-synthesis scaffolds with nano-grade, thus it has some advantages and give a new way for the development of tissue engineering. At the end, the problems of these scaffolds, their trend of development and perspective studies were discussed.

With the change in the modes of medicine, the traditional educational mode of clinical medicine is unable to meet the development demands of modern medicine. Evidence based medicine, a new mode of medical treatment, reflects the development trends of modern medicine and represents the direction of modern medical advancement. Judging from the perspective of medical education, evidence based medicine is a learning method which differs from the traditional educational mode and represents a new concept of education in clinical medicine. The rise of evidence based medicine demonstrates the direction of reform in medical education in the world today and will also bring about great changes in the mode of medical education in China. It is imperative for us to follow the trend in clinical medical education, update concepts on medical education in line with the basic ideas of evidence based medicine, and push forward reform in the mode of medical education.

Objective To evaluate the long-term efficacy and safety of thermal balloon endometrial ablation (TBEA) for patients with menorrhagia. Methods From February 2004 to January 2008,105 women with menorrhagia was admitted to our hospital and treated by TBEA. The clinical data,including menstrual cycle and blood volume,as well as TBEA complications such as abdominal pain,infection,bleeding and conglutination,were followed up for a long period in the patients. Results Among the 105 patients,two were lost for follow-up,and one died of malignant tumor of the urinary system in 56 days after the TBEA,the other 102 (97.1%) patients achieved an follow-up for 3 (102 patients),6 (101 patients),12 (99 patients),24 (66 patients),or 36 months (51 patients) succesively. At each of the time point,the success/amenorrhea rates were 93.1% (95/102)/38.2% (39/102),93.1% (94/101)/33.7% (34/101),91.9% (91/99)/32.3% (32/99),93.9% (62/66)/34.8% (23/66),and 92.2% (47/51)/31.4% (16/51),respectively. No severe perioperative complications such as perforation of the uterus and adjacent organs,massive bleeding or infection due to mechanical or thermal injuries occurred. After the operation,11 (10.5%) patients had uterus contraction-like pain,which was relieved by analgesic drugs within 6 hours;8 (7.9%) patients had adhesion of the tissues around the cervix uteri and/or uterine cavity,4 of them were cured by dilation of the uterus,3 received surgical separation,and 1 underwent resection of the uterus. No significant difference in the effective rate and rate of amenorrhea was found between the 36-and 12-month follow-up results (?2=0.000,P=1.000 and ?2=0.014,P=0.906,respectively),or between 24-and 12-month follow-up results (?2=0.034,P=0.854;?2=0.114,P=0.736). Conclusion TBEA shows good long-term efficacy and safety,as an applicable alternative in the treatment of menorrhagia.