Objective To understand the drug tolerance of Augmentin for Escherichia coli , Klebsiella pneumoniae spp and other pathogens Methods The MIC, MBC and bactericidal curves of Augmentin of 135 clinically isolated pathogenic bacteria were assayed The anti bacterial activity and clinical effect between Augmentin, Cefurxine, Ceftriaxone, Amoxicillin and Cefazolin were compared by MIC 50 , MIC 90 , MBC 50 , MBC 90 and modal number MIC,MBC Results Augmentin was better than the same kind of antibiotic without ? lactamase inhibitor It had a good effect for anti bacteria activity of extend spectrum ? lactamase (ESBLs) Escherichia coli and Klebsiella pneumoniae spp Conclusion Augmentin can be used for infection of ESBLs and other common pathogenic bacteria, since it has a good anti bacterial activity for most clinicaly isolated pathogenic bacteria and productive enzyme bacteria

Objective To investigate the morphological characteristics of vertebral-basilar artery in patients with posterior circulation ischemia vertigo. Methods The data of cerebrovascular disease related risk factors,neck CT angiography,and head MR angiography of 122 patients with posterior circulation ischemia vertigo and 60 non-vertigo volunteers with cerebrovascular disease related risk factors over the same period were analyzed retrospectively. The positive index of single factor comparison was analyzed by using multivariate logistic regression analysis. The risk factors for posterior circulation ischemia vertigo were screened,and the degree of vertebral artery stenosis and the degree of basilar artery tortuosity of the posterior circulation ischemia group were compared with those of the control group. Results (1 )The incidences of hypertension,hyperlipidemia,previous history of stroke or transient ischemic attack,vertebral artery atherosclemtic stenosis,vertebral artery variation,and basilar artery tortuosity of the posterior circulation ischemia group were 59%(n=72),55. 7%(n=68),64. 8%(n=79),41. 0%(n=50),28. 7%(n=35),and 30. 3%(n=37),respectively,and they were all significant higher than 33. 3%(n=20),31. 7%(n=19),35. 0%(n=21),15. 0%(n=9),10. 0%(n=6),and 15. 0%(n=9)of the control group. There were significant differences (all P<0. 05). (2)Vertebral artery atherosclerotic stenosis (OR,3. 891, 95%CI 1. 721-8. 800,P <0. 01),vertebral artery variation (OR,3. 231,95%CI 1. 238-8. 432,P =0.017),and basilar artery tortuosity (OR,2. 664,95%CI 1. 189-5. 972,P=0. 017)were the independent risk factors for posterior circulation ischemic vertigo. The degrees of vertebral artery stenosis and basilar artery tortuosity of the posterior circulation ischemic group were higher than those of the control group. There were significant differences (all P<0. 05). There was significant difference in the vertebral artery dominance between the degree of basilar artery tortuosity ≥grade 2 and

Objective: To study the role of IL-6 in the dental pulp indirect injury by gunshot. Methods: The mandible was wounded by gunshot at (961.18?8.94) m/s in 17 dogs.2 h,6 h,1 d,3 d,7 d after injury molars,caspids,and lateral incisors were sampled respectively for IL-6 detection by immunohistochemical method in dental pulp. Results: The results showed that positive staining of IL-6 was observed predominantly in infiltrating inflammatory cells, vascular endothelial cells and some fibroblasts,IL-6 expression was consistent with the recruitment and activation of inflammatory cells, and with the severity duration of pulpal tissue injury and response. IL-6 level was increased in the dental pulp 6 h～3 d after injury.Conclusion: IL-6 is involved in regulating physiologic responses of dental pulp injuries in maxillofacial trauma by a projectile.

Objective To investigate the diagnosis value of laparosco-pie examination in ascites.Methods The data of twenty-three cases with ascites of unknown origin undergoing laparescopie examination and pathologic examination were retrospectively analyzed from May 2004 to August 2008.Results Twenty-two eases were definitely diagnosed by laparoscopie exploration,the positive rate was 95.6%.One ease re-mained unknown.Among them,18 eases were tuberculous peritonitis; 3 eases were metastatic adenocarcino-ma; one ease was peritoneal mesothelioma.No obvious postoperative complications occurred.Conclusion Laparoscopie examination is a safe method with hige definite diagnostic rate,which has important values in diagnosis of ascites of unknown origin.

Objective To investigate the feasibility and safety of laparoscopic cholecystectomy(LC) in patients with chronic renal dysfunction at azotaemic stage.Methods Clinical data of 7 patients accompanying chronic renal dysfunction at azotaemic stage treated with LC between May 2004 and September 2006 were analyzed retrospectively.The operation was performed under general anesthesia and endotracheal intubation.The CO2 pressure was maintained at 9～12 mm Hg.The LC was conducted with 3-port technique in 5 patients and 4-port technique in 2.Results The LC was completed smoothly in all the 7 patients.Patients' renal dysfunction was not aggravated.There was no significant difference between pre-and post-operative time in levels of blood urea nitrogen(11.92?4.06 mmol/L vs 12.16?3.76 mmol/L;t=0.50,P=0.633) and blood creatinine(208.62?134.37 ?mol/L vs 204.20?125.53 ?mol/L;t= 0.51,P=0.626).As compared with preoperative levels,the creatinine clearance rates were not significantly changed at 2 weeks after operation in 3 patients(40.03 ml/min vs 45.61 ml/min;32.28 ml/min vs 38.93 ml/min;56.72 ml/min vs 51.60 ml/min).Follow-up checkups for 4～20 months(mean,10 months) showed no aggravation of renal dysfunction.Conclusions Laparoscopic cholecystectomy is a feasible and safe procedure for patients with chronic renal dysfunction at azotaemic stage.

The risk factor of dental fluorosis are mainly related to the cancen tration of fluoride in the drinking water,the supplements of fluoride,nutrition states and regime,the time of fluoride exposure.Other factor such as the period of mother's milk feeding,the altitude of resident,the environmental pollution,the education degree of parents,and the level of health service of the community are also contributed to the development of dental fluorosis.Besides,the knowledge of dentists,the health habits of individual,the condition of economic,and so on is somehow the risk factor of dental fluorosis.The chances of fluoride exposure will become more often as the prosperous of the society.Thus any health policy of the complement of fluoride should be adapted to local conditions.

Objective To study the feasibility of laparoscopic cholecystectomy accompanied by simultaneous umbilical hernia repair.Methods Twenty-four adult patients received laparoscopic cholecystectomy accompanied by simultaneous umbilical hernia repair in our hospital from November 2003 to April 2008.An incision was made in navel defect and entered the abdominal cavity under a direct vision.LC was performed by 3-trocar technique.Umbilical hernia was repaired by Mayo' style.Results The operation was successfully completed in all the cases.The operation time ranged from 35 to 65 minutes (mean,48 minutes),the blood loss was 5-30 ml(mean,16 ml),and hospital stay after the operation was 3 -4 days.No patient dereloped wound infection or skin necrosis.No recurreme was found during a 1-to 54-month follow-up(mean,28.6 months).Conclusions Laparoscopie cholecystectomy accompanied by simultaneous umbilical hernia repair is a safe and effective procedure.Mayo repair is simple and effective for umbilical hernia with less fascial defects.

Objective:To investigate effects of strengthening the spleen and tonifying the kidney and their combination before and after exercise on relative indexes of metabolism of glucose and amino acids.Methods:Strengthening the spleen alone,tonifying the kidney alone or their combination before and after exercise was given to the rat receiving 7-week gradually increasing load exercise on a running platform,and after quantitative load,contents of serum lactic acid,urea nitrogen,glucose,hepatic glycogen,muscle glycogen,hemoglobin,and malondialdehyde(MDA)in renal tissue and activity of superoxide dismulase(SOD)in the renal tissue were determined.Results:Strengthening the spleen before exercise increased hepatic and muscle glycogen contents,and tonifying the kidney before exercise increased muscle glycogen content,and strengthening the spleen combined with tonifying the kidney before and after exercise increased hemoglobin level,strengthening the spleen followed by tonifying the kidney before exercise decreased serum lactic acid and urea nitrogen levels,increased muscle glycogen content and SOD activity/MDA content ratio in the kidney.Conclusion:Strengthening the spleen and tonifying the kidney play a certain role in reducing motion-related fatigue,while strengthening the spleen followed by tonifying the kidney before exercise has the best results.

Adult ; Female ; Gas Poisoning ; drug therapy ; Glycoproteins ; therapeutic use ; Humans ; Male ; Middle Aged ; Quinuclidines ; therapeutic use ; Treatment Outcome ; Young Adult

Objective To obtain recombinant human granulysin using prokaryotic expression system.MethodsTotal RNA was extracted from cultured PBMC. Granulysin gene segments were obtained with granulysin-specific primers by RT-PCR and then inserted into pET32a(+) plasmid. After identification by DNA sequence,pET-GNLY9K and pET-GNLY15K were transferred to E. coli Rosetta (DE3). The fusion protein was identified by SDS-PAGE and Western blot.The bioactivity of granulysin fusion protein was measured by MTT assay.Results The prokaryotic expression vectors pET-GNLY9K and pET-GNLY15K were successfully constructed.The corresponding protein was highly expressed in E.coli. Recombinant protein was specifically bound by anti-granulysin antibody. GNLY9K fusion protein significantly inhibited the growth of A549 cells in a dose-dependent manner,while GNLY15K had little effect on the growth of A549.Conclusion Granulysins with different mw were successfully expressed using prokaryotic expression system,which might be helpful for the further study of granulysin.