OBJECTIVE:To establish an HPLC method for simultaneous determination of Naringin,Hesperidin and Neohesperidin in the effective fraction of flavone in Fructus Aurantii Immaturus.METHODS:Hypersil C18 column(250 mm?4.6 mm,5 ?m) was used with the mobile phase consisted of acetonitrile -0.1% phosphoric acid solution(20∶80) at a flow rate of 1.0 mL?min-1,and the detection wavelength was set at 283 nm and the column temperature was maintained at 30 ℃.RESULTS:The calibration curves of Naringin,Hesperidin and Neohesperidin were in good linearity over the ranges of 0.44～2.20 ?g(r=0.999 5),0.025 6～0.128 0 ?g(r=0.999 3),0.54 ～ 2.70 ?g(r=0.999 9),respectively.And the average recoveries for the three constituents were 99.41%,100.33% and 99.69%,respectively with RSD at 1.14%,1.47%,and 1.16%,respectively.CONCLUSION:The method is simple,accurate and reliable,and can be used for the quality control of Fructus Aurantii Immaturus.

Adult ; Athletes ; Electroencephalography ; Female ; Humans ; Male ; Sports ; physiology

AIM: To discuss the effects of preoperative vitreous cavity injection of ranibizumab on 25G minimally invasive vitreoretinal surgery (VRS) in patients with proliferative diabetic retinopathy(PDR).METHODS: From February 2013 to December 2015,74 patients with PDR received VRS (82 eyes) were retrospectively analyzed.Patients were divided into VRS + IVR group and VRS group according to preoperative VRS treatment.VRS+IVR group: preoperative injection of ranibizumab (10mg / mL) at 3-5d before operation;VRS group: VRS only.We recorded VRS operation time,filling situation,iatrogenic retinal hole and so on.RESULTS: The operation time and coagulation times of VRS+IVR group were significantly lower than VRS group.The two groups in operation time and coagulation times were statistically significant (all P<0.05).In the VRS+IVR group,the number of cases of iatrogenic retinal hole and the use of silicone oil were lower than those of VRS group,the difference between the two groups was statistically significant (P<0.05).While the use number of C3F8 and perfusion fluid were not statistically significant (P>0.05).In VRS-IVR group within 3mo after operation,4 eyes appeared to vitreous body hematocele,VRS group was 13 eyes,the difference was statistically significant (x2=4.966,P<0.05).CONCLUSION: PDR patients with 25G minimally invasive VRS with preoperative therapy can reduce postoperative ocular bleeding,rate of retinal injury and silicone oil filling,shorten the operation time,the incidence rate and improve the success rate of surgery and reduce eye bleeding,postoperative complications such as adhesion.

Objective To investigate perinatal outcomes of twin pregnancy.Methods Perinatal outcomes of 658 pregnancies with twins hospitalized and born at Beijing Obstetrics and Gynecology Hospital during 2002 to 2005 were retrospective analyzed to compare the influence of type of twin,delivery mode, gestational week,birth time interval between twins,as well as specific complications of twin pregnancies on their outcomes.Results Pefinatal fatality of monzygous twins was 53.69 per thousand(16/298),higher than that of dizygous twins(27.11 per thousand,9/332),with U=2.35,P0.05.Perinatal fatality was higher in twins with different development (birth weight).Perinatal fatality in dizygous twins(12.5 percent,2/16)was lower than that in monozygous ones(14.3 percent,6/42),and that in twins with different development(birth weight)(0.6 percent, 2/316)was lower than that with same development(1.2 percent,3/256),with ?~2=16.944,P

OBJECTIVETo observe the effect of jujube pretreatment on serum levels of AST and ALT, liver pathology, and the expression of cytochrome P4502e1 (CYP2E1) and tumor necrosis factor-alpha (TNF-alpha) in the liver tissue of alcoholic liver disease (ALD) mice.

METHODSTotally 88 Kunming mice were randomly divided into the control group (n = 28), the model group (n = 32), and the jujube treatment group (n = 28). The animal model was prepared using intragastric alcoholism for mice in the model group and the jujube treatment group, while distilled water was intragastrically given to those in the control group. Extraction of jujube was intragastrically given to mice in the jujube treatment group at week 4, while equal volume of distilled water was intragastrically given to mice in the rest two groups. The therapeutic course lasted for 12 weeks. Serum levels of AST and ALT, liver pathology, and the expression of CYP2E1 and TNF-alpha in the liver tissue of ALD mice were observed after administration of jujube.

RESULTSCompared with the model group, serum levels of AST and ALT decreased, the liver pathology was improved, and the expression of CYP2E1 and TNF-alpha in the liver tissue decreased, showing statistical difference (P < 0.05).

CONCLUSIONJujube had certain effect in treating ALD.

Animals ; Cytochrome P-450 CYP2E1 ; metabolism ; Disease Models, Animal ; Liver ; drug effects ; metabolism ; Liver Diseases, Alcoholic ; drug therapy ; metabolism ; Male ; Mice ; Mice, Inbred Strains ; Plant Extracts ; pharmacology ; Tumor Necrosis Factor-alpha ; metabolism ; Ziziphus ; chemistry

Adult ; Bone Neoplasms ; pathology ; surgery ; Femur Neck ; pathology ; Humans ; Male ; Osteochondroma ; pathology ; surgery

Acupuncture Therapy ; Adult ; Aged ; Bloodletting ; Combined Modality Therapy ; Crying ; Female ; Humans ; Laughter ; Male ; Middle Aged ; Stroke ; psychology ; therapy ; Treatment Outcome

Objective To evaluate the effects of a health education project on prevention and control of iodine deficiency disorders.Methods From January to June 2011,an investigation was conducted using combined unified questionnaire in 9 counties on target population,and 3 townships were chosen in each project county.Health educational activities were carried out in grade 5 classes in the central primary school.In the meantime,3 villages were chosen in each selected township where the health educational activities in the community were carried out.Before and after the health educational activities,questionnaire surveys on knowledge of iodine deficiency disorders were conducted in each project county.Thirty students in one class of grade 5 in the central primary school were selected,and 15 women of childbearing age near the central primary school were selected too.The health education content included but not limited to:popular science films about the knowledge on endemic disease prevention and public-interest ads were broadcasted by radio,television,newspapers and other media in the project counties,aiming at spreading knowledge on prevention and treatment of endemic diseases.In each of the project township government and village committee locations,posters,banner pieces and slogan suspensions about knowledge on endemic disease prevention were posted or put up.In the project rural hospitals,a special bulletin boards was set up,and in the village clinics (rooms),posters were put up,to carry out a training program for women of childbearing age on endemic disease prevention.In primary schools,students in grades 4 to 6 were given a class about knowledge on prevention and treatment of iodine deficiency disorders,aiming at their transferring the learned knowledge to family members.Results A total of 2678 persons were investigated before and after the health education.After health education,the rate of knowledge(the correct answer rate) on iodine deficiency disorders increased from 69.04％ (1920/2781) to 94.08％ (2622/2787,x2 =580.63,P ＜ 0.05) among primary school students,and 74.76％(924/1236) to 92.60％(1139/1230,x2 =143.59,P ＜ 0.05) among women of childbearing age.Conclusions The rate of knowledge awareness in the students and the women of childbearing is increased significantly after the health educational activities.Systematic and targeted health education activities are effective measures in prevention and control of iodine deficiency disorders.

BackgroundThere are a lot of studies about the wound healing charateristics of cornea after SBK with femtosecond laser.In our study,mechanical microkeratome was preferred for corneal flap.We observed the proliferation of keratocytes by investigating the myofibroblast ( MF) activity.Objective The study was to compare the morphologic and histological changes in the cornea after sub-Bowmans keratomileusis ( SBK) with photorefractive keratectomy ( PRK) and laser in situ keratomileusis ( LASIK)by investigating the express of transforming growth factor-β( TGF-β)and α-smooth muscle actin( α-SMA)and to investigate the wound healing characteristiCs of cornea after SBK.MethodsTwenty-seven adult New Zealand white rabbits were randomly assigned into group A,B and C.SBK waa performed on the right eyes of each rabbit in group A,LASIK for group B and PRK for group C.All the left eyes were used as the normal control group.Histological examinations by light microscopy were performed on day 7,1 months,3 months after surgery.The expression of α-SMA and TGF-β and the number of activated MF were assessed by immunohistochemiatry.ResultsIn SBK group,corneal epithelium cells proliferation around the wound was seen and the numbers of active fibroblasts were increased after surgery.The expression of α-SMA or TGF-β around the corneal flap and the corneal 8troma started at day 7 postoperatively and peaked at 1 months and decreaaed around the corneal flap and the corneal 8troma started at day 7 postoperatively and peaked at 1 months and decreaaed t3mnh.TFβep( SBK:t=2.226,2.158,2.330,P＜0.05;PRK:t=4.745,6.524,6.293,P＜O.05).The numbersof activ( SBK:t=2.226,2.158,2.330,P＜O.05;PRK:t=4.745,6.524,6.293,P＜0.05).The numbersof activatedMFs were different fromLASIKstatisticallytoobetweenSBKgroupandPRKgroup ( SBK:t =4.439.5.692,4.175,P＜0.05 ; PRK:t=6.330,6.723,5.267,P＜0.05 ).Theα-SMAand TCF-βexpressionsin SBKgroupwerelessthan PRK group but more thanLASIKgroup( TCF-β:t =4.691,5.527,t =4.399,P＜0.05 ; α-SMA:t =9.637.10.282,8.197,P＜0.05).The numhers of MFs in SBK group was less than PRK group before 3 months and were same at 3 months ( t =5.188,4.529,P＜0.05 ).Conclusions ComparedwithconventionalLASIK,SBKcanup-regulatethe expression of α-SMA.TGF-β,activated MFs in the corneal flap.which enhance corneal biomechanics and promote healing.However,most of the disadvantages caused by wound healing in SBK still remain compared to PRK.

Objective To explore the changes and relationship of serum insulin-like growth factor-1(IGF-1) and leptin in children with growth hormone deficiency(GHD).Method Serum IGF-1 and leptin levels were measured by immunoradiometric assay in 20 prepuberal normal children and 23 children with GHD.Results Serum IGF-1 level in GHD group(51.158?29.988)?g/L was significantly lower than that in controls(112.680?41.540)?g/L.Serum leptin levels in GHD group(6.002?2.204)?g/L was significantly higher than that in controls(4.523?2.204)?g/L.There was no significant correlation between serum IGF-1 and leptin.Conclusion IGF-1 and leptin regulate growth in children with GHD in their individual way.