AIM:To investigate the effects and possible mechanisms of baicalin on the atherosclerosis rabbits.METHODS:Thirty healthy New Zealand white rabbits were divided randomly into five groups:normal control group(A group,n=6)and fed with normal diet,atherosclerosis model group(B group,n=6),baicalin therapic group(C group,n=6),bacalin preventive group(D group,n=6),and positive control group(E group,n=6),the last four groups fed with hypercholesterol diet for 12 weeks.Treated with bacalin 300 mg/(kg?d),and simvastatin 5 mg/(kg?d)additionally for 2 weeks in C and E group respectively from the tenth week.Treat with bacalin 100 mg/(kg?d)for 12 weeks in D group.Serum lipids and liver lipids were detected with standard enzymatic assays.Atherosclerotic plaque/intima size ratio of area and NF-?B content in blood vessel tissue were examined.Enzyme-linked immunosorbent assay(ELISA)was employed to monitor the levels of serum TNF-?,IL-1? and Adiponectin.RESULTS:Atherosclerotic plaque/intima size ratio of area decreased in C,D and E group than in B group(P

Arteriosclerosis Obliterans ; surgery ; Humans ; Lower Extremity

Objective To establish the method of high performance liquid chromatography and hydrid genesis atomic fluorescence spectroscopy(HPLC-HGAFS) about arsenic species in urine of rats treated with sodium arsenite.Methods The solution containing 15 mmol/L(NH4)2HPO4(pH=6.0)was used as the chromatographic eluant,the flow rate of which was 1.0 ml/min.The parameters of HPLC-HGAFS consisted of multiplier negative high voltage,hollow-cathode lamp's joint current and co-current,the flow rate of carrier gas,the flow rate of barrier shield gas,supporting liquid and reducing agent,which were 285 V,80 mA,36 mA,400 ml/min,600 ml/min,7%HCl and the mixed liquor of 1.5%KBH4 and 0.35% KOH respectively.Results The linear range of method was 20-100 ?g/L,and the linearity of regression equation of iAs3+,iAs5+,DMA was better.The lowest detection concentration of iAs3 +,iAs5 +,DMA in urine were 12.03,6.67 and 8.66 ?g/L respectively and all of the coefficient correlation were ≥0.98.The average recovery rates of DMA were 96.5%-103.4% and RSDs were 0.39%-1.26%.Conclusion The method has been proved accurate,sensitive and is applicable to the determination of arsenic species in urine.

Objective To investigate the status of diabetes and its risk factors in Karamay.Methods A sampling survey was carried out in Baijiantan district of Karamay from March,2003 to Dec.,2003,all subjects aged 20 to 75 and lived 5 years and above were selected with stratified sampling method according to the age structure of the inhabitants in Karamay.Results Diabetes prevalence was positively correlated with age(OR=1.047),male(OR=1.922),obesity(OR=1.641),hypertension(OR=1.624) and hyperlipidemia(OR=2.131)(P

Objective:To observe the expression of nuclear factor-kappa B( NF-κB) in rats with acute lung injury ( ALI) caused by oleic acid ( OA) and investigate the protective effect of puerarin ( Pue) in ALI. Methods:Twenty-four rats were randomly divided into three groups:the control group, OA group and Pue group. Morphological changes in lung tissues were observed under a light mi-croscope and the expression of NF-κB in lung tissues were examined. Results:Morphological changes in lung tissues showed that pul-monary inflammation was relieved in Pue group compared with that in OA group. Compared with the control group, the expression of NF-κB was increased obviously in OA group (0. 39 ± 0. 07 vs 0. 12 ± 0. 04, P<0. 05), and that was decreased obviously in Pue group (0. 24 ± 0. 05), which was still higher than that in the control group (P<0. 05). Conclusion:Puerarin can alleviate the inflammation reaction in ALI through inhibiting the expression of NF-κB.

Objective To investigate the effect of different intestinal cleaning methods on bowel preparation for laparoscopic peritoneal vaginoplasty.Methods One hundred patients undergoing laparoscopic peritoneal vaginoplasty were divided into experiment group and control group with 50 cases in each group .For bowel preparation,the experiment group was managed with oral sodium phosphate salt solution of 45 mL and the control group with oral senna leaf of 30 g in boiled water.The two groups were compared in terms of intestinal cleaning degree,adverse reactions and postoperative exhaust time.Results The incidence of adverse reactions in the former group was significantly lower than the control group.Degree of bowel cleaning satisfaction was significantly better than that of the control group and the postoperative exhaust was significantly earlier than that in the control group(P<0.05).Conclusion The method of oral sodium phosphate salt solution for bowel preparation for laparoscopic peritoneal vaginoplasty can improves the intestinal cleanliness,reduce incidence of intestinal adverse reaction and promote postoperative exhaust so that it can improve postoperative intestinal restoration of kinetic energy.

Objective To prepare celastrol ethosomes and to observe the permeability characteristics of the ethosomes which act as the transdermal delivery carriers of celastrol in vitro. Methods Celastrol ethosomes were prepared by ethanol injection method, and then the encapsulation efficiency, particle size, polydispersity index ( PDI) and zeta potential of the ethosomes were analyzed. TP2A intelligent percutaneous penetration instrument was used to compare the skin penetration properties of celastrol ethosomes, celastrol solution and the mixture of blank ethosomes with celastrol solution. Results The prepared celastrol ethosomes were spherical, and the average particle size was (401.3 ± 5.5) nm, PDI was 0.21± 0.02, steady zeta potential was (-2.75 ± 0.1) mV, and average encapsulation efficiency was ( 80.6 ± 0.7) %. The amount of accumulative penetration of celastrol ethosomes at 48 h was 76.86 μg·cm -2 and the permeation rate was 1.640 9 μg·cm -2·h -1, which were significantly higher than the celastrol solution and the mixture of blank ethosomes with celastrol solution. Conclusion The prepared ethosomes have high encapsulation efficiency, uniform particle size and stable quality, and are beneficial to the transdermal absorption of celastrol.

AIM: To study the mostly pharmacological effects of Kangjun Xiaoyan Tablets (Flos Lonicerae, Radix Stemonae, Radix et Rhizonia Rhei, etc.) METHODS: The pharmacological functions of KJXYT were observed by measuring its antibacterial, anti-inflammatory, antipyretic and immune etc. RESULTS: KJXYT had remarkably protected the mice from the infection with streptococcus pneumococcus and staphylococcus aureus; had dramatic anti-inflammatory effect on the inhibited feet edema of rat induced by egg white and the ear edema of mice induced by dimethylbenzene; and had obvious effect on fever of rats caused by dried yeast; besides, and strengthened the phagocytosis of mice’s reticuloendothelial system. CONCLUSION:KJXYT serves the function of antibiosis, anti-inflammation, antipyresis and immunpotentiation. enedth

AIM: To establish the method for quality control of Gutongning Liniment(Herba Epimedii,Semen Strychni,Herba Ephedrae,etc.) METHODS: Herba Ephedrae,Radix Notoginseng,Ramulus Cinnamomi,Flos Caryophylli,Flos Daturae were identified by TLC,icariin content in Herba Epimedii and strychnine content in Semen Strychni were determined by HPLC. RESULTS: The identified characteristics of chromatography were distinct and the spots were clear.Icariin showed a good linear relationship at a range of 0.2-1.2 ?g,r=0.999 8.The(average) recovery was 10.3%,and RSD was 1.5%.Strychnine showed a good linear relationship at a range of 0.8-4.8 ?g,r=0.999 7.The average recovery was 99.17%,and RSD was 1.4%. CONCLUSION: The methods are accurate and reliable,and can be used to control the quality of this preparation effectively.

Objective We analyzed the curative effect of ERCC1 and RRM1 expression on the Neo-adjuvant Chemotherapy of stage Ⅲ NSCLC to investigate the guiding function of ERCC1 and RRM1 expression in chemotherapy regimen containing platinum.Methods Branch DNA-liquid phase chip methods were used to detect ERCC1 and RRM1 expressions before chemotherapy in 80 cases of stage Ⅲ NSCLC confirmed by pathology.All patients received 2 periods Neo-adjuvant Chemotherapy with GP regimen.According to WHO efficacy appraisal standard,the Enhanced Scan of CT showing reaching complete remission or partial remission was effective or stable,otherwise the progression was considered ineffective.Results For the 80 cases of stage Ⅲ NSCLC,the treatment for 20 of the 25 patients with low expressions of both ERCC1 and RRM1 were effective with an effective rate of 80.0％;The treatment for 14 of the 23 patients with low expressed ERCC1 and high expressed RRM1 were effective with an effective rate of 60.9％;The treatment for 10 of the 20 patients with high expressed ERCC1 and low expressed RRM1 were effective with an effective rate of 50.0％;and the treatment for 4 of the 12 patients with both high expression were effective with an effective rate of 33.3％.The difference of effective rates among the four groups had statistical significance ( x2=7.81,P＜0.05 ) with group A having significantly higher rate than the other three groups and group B and group C having significantly higher rate than group D ( P＜0.05 ).Conclusion ERCC1 detection has guiding significance on the regimen selection of NSCLC Neo-adjuvant Chemotherapy.It was worthwhile to use ERCC1 detection widely in the individualized treatment of the stage Ⅲ NSCLC before surgery.