Physical performance is an ability with which the sufferers of lumbar disc herniation(LDH) achieve daily work and basic physical activity,including daily activities(such as the diverting movement from standing to sitting),functioning state of nerves and muscles,dailyworking ability and walking gait,etc.;abilities of body performance relative to pains,such as walking ten meters with loads,sit-stand test and Tread mill walk,etc.Most of current rehabilitation evaluation may be influenced by the subjective factors of the examinee or the examiner,but intelligent device for energy expenditure and activity(IDEEA) may be helpful to evaluate physical performance of the LDH patients in a complete,quantity-fixing,systematic and dynamic way.

Objective:To investigate the effects of Mycobacterium Phlei on functional states of Th_1/Th_2 in patients with chronic obstructive pulmonary disease(COPD),and clinical effects on the prevention of acute exacerbation of chronic obstructive pulmonary diseases(AECOPD).Method:60 patients with COPD of gradeⅡwere randomly divided into a treatment group and controlled group.The controlled group were given a routine therapy,while the treatment group were given utilins in addition to the routine treatment.The contents of?-interferon(IFN-?) and interleukin-4(IL-4) in their peripheral blood and their clinical efficacy were observed before and after the treatment.Result:Before and after the treatment, IFN-?/IL-4 ratio in the treatment group was 1.39?0.20 and 1.94?0.31 respectively(P

Purpose: To study the influence of the manual manipulations on the reversal ability of the skull space in the patients with vertigo due to proprioceptive disturbance of the neck. Methods: By motor surveyor of skull three-dimensional space, the changes in the reversal ability of the skull space before and after the treatments with manual manipulations were observed in 72 cases of the patients with vertigo due to proprioceptive disturbance of the neck. Besides, 51 cases of vertigo due to insufficient blood supply of the cervical artery and 138 normal adults were selected as the control group. Results:After the treatments with manual manipulations, the deviation degree of the skull space in the three motor axes were all obviously decreased in the proprioceptive group, more obviously in the flexing and extending direction. The deviation degree of the skull space was also decreased in the ischemic group in the flexing and extending direction. Conclusion: Manual manipulations have the instant function to improve the proprioceptive function of the neck, and the factor of the proprioceptive disturbance also exists in the clinical onset of vertigo in pattern of insufficient blood supply of the cervical artery.

Objective To investigate the optimal combination and the value of functional magnetic resonance imaging in the diagnosis of breast tumors. Methods One hundred and forty patients who had been clinically and pathologically diagnosed as breast tumors in our hospital during the period of 2009 to 2013 were collected. 63 of whom had breast cancer and 77 had benign tumor. All the patients received 1H-MRS, DWI and dynamic enhanced MRI at the same time. The obtained images were analyzed and then compared with the pathological findings. The accuracy, sensitivity, and specificity of MRS combined with DWI or dynamic enhanced MRI, or DwI combined with enhanced MRI in the diagnosis of breast cancer were obtained. Results All the diagnosis were pathologically confirmed. The accuracy, sensitivity, and specificity were 89.8%, 90.1%, and 90.0% for DWI combined with dynamic contrast-enhanced MRI; 77.9%, 77.3% and 79.3% for MRS in combination with enhanced MRI; and 76.3%, 77.8% and 77.1% for DWI combined with MRS. There were significant differences among the three kinds of combination detection in diagnosis of breast cancer , χ2= 9.057, P = 0.011. Conclusions The sensitivity, specificity and accuracy is the highest in DWI combined with dynamic enhanced MRI in the diagnosis of breast tumor , which can be used as the best combination imaging for breast tumor.

Objective To observe the efficacy of CTLA-4Ig gene local transfection on the survival of renal allografts. Methods The kidneys of BN rats were transfected with CTLA-4Ig gene-recombined adenovirus. The transfected kidneys were transplanted to Lewis rats (transfection group). BN→Lewis kidney transplantation with non-transplanted kidneys served as controls. The allograft survival time and the allograft function between the two groups were compared. Results The allograft survival time of transfection group was longer than that of controls significantly (32? 8.0?days vs 8.5 ? 1.4?days , P

Objective To investigate the feasibility of endothelial reconstruction in the injuried arterial wall by autologous endothelial cells transplantation. Methods New Zealand white rabbits (n=20) were subjected to bilateral iliofemoral artery balloon injury, Cultured autologous venous endothelial cells were immediately transplanted by balloon catheter into one vessel, whereas the contralateral artery received the medium only. In 10 rabbits, vessels were harvested 4 hours and 4 days after transplantation for analysis of endothelial coverage by scanning electron microscope (SEM); In 5 rabbits, the cultured endothelial cells were labeled with a fluorescent tracer before their introduction into the injured vessel, and 4 days after transplantation, vessels were harvested to obtain fluorescent imaging of the seeded endothelial cells; Another 5 rabbits were sacrificed 4 days after transplantation for Evans blue staining. Results Four hours after the operation, SEM demostrated that the endothelial layer in control vessels were denuded completely, whereas some round endothelial cells had adhered into the aterial wall in cell transplantation group; four days after cell transplantation, the transplanted cells had attached and spread in the injuried aterial wall by SEM, a number of fluorescent labeling endothelial cells were observed in the endothelial denuded aterial wall, the vessels that received the medium only were stained nearly completely by Evans blue, whereas in those vessels that received cell transplantation, about 60% area were not stained. Conclusion Autologus endothelial cells can be effectively transplanted into the injuried arterial wall by balloon catheter.

BACKGROUND:Relative to blood samples, mouth swab samples are more beneficial for apolipoprotein E gene polymorphism analysis among large cohorts. However, agreement has not yet been reached about how to extract genomic DNA form mouth swab samples.
OBJECTIVE:To develop an appropriate method to extract genomic DNA form mouth swab samples, which are suitable for apolipoprotein E gene polymorphism analysis.
METHODS:Fifty mouth swab samples from patients with sporadic Alzheimer’s disease were col ected. Magnetic nanoparticles and PicoDNA trace nucleic acid extraction kit were used to extract genomic DNA form mouth swab samples. And the purity and concentration of the genomic DNA extracted by the two methods were analyzed. Then PCR amplifications and DNA electrophoresis were performed to confirm whether the genomic DNA was able to amplify desired DNA fragments. DNA sequencing was applied to analyze apolipoprotein E gene polymorphisms.
RESULTS AND CONCLUSION:Genomic DNA extracted by the two methods was of high purity. The concentration of genomic DNA extracted by magnetic nanoparticles was higher than by PicoDNA trace nucleic acid extraction kit, and the difference had statistical significance (P<0.05). Al the genomic DNA were able to performed PCR amplifications to obtain desired PCR products, but results of DNA electrophoresis showed that DNA fragments were more clear by nanoparticles method. The results of DNA sequencing were the same by the two methods. The distribution ofε2,ε3,ε4 genotypes of apolipoprotein E gene was 6%, 71%, 23%, respectively. Magnetic nanoparticles were better than PicoDNA trace nucleic acid extraction kit for extracting genomic DNA form mouth swab samples for apolipoprotein E gene polymorphism analysis.

Objective To investigate the hemodynamics and structural effect of rat endothelial progenitor cells (EPC) transplant on pulmonary artery hypertension (PAH) induced by monocrotaline(MCT) in rats.Methods EPCs were identified and marked.Twenty-one days after injection of EPCs,the pulmonary hemodynamic parame- ters,average pulmonary artery pressure (mPAP),right heart index were determined.The vascular endothelial cells and pulmonary vascular structural changes were verified by fluoresccuse microscope.Results Compared with the model,EPCs treatment(n=10) decreased mPAP significantly (mPAP,EPCs:25.9?0.7 mmHg vs model group:29.3?2.2 mmHg,P

Objective To understand the status and drug resistant patterns of strains of extended spectrum ?-lactamase(ESBLs) in children with lower respiratory tract infection,and to give clinical suggestions for rational treatment.Methods Escherichia coli and klebsiella pneumoniae were isolated from the 2 969 nasopharyngeal secretions which collected from lower respiratory tract of children in our hospital from Jan.2006 to Dec. 2007.Dual-sheets and sheets-diffusing method (K-B method) were used to determine the ESBLs and antibiotic susceptibility was tested by K-B method which included 18 kinds of antibiotics,the results were marked by resistant,intermedial and sensitive.Chi-square test was used to analyze the data.Results Total 135 strains were detected,73 strains were escherichia coli,of which 54 strains(74.0%)produced ESBLs,62 strains were klebsiella pneumoniae,of which 33 strains(53.2%)produced ESBLs.The 2 bacterias were found more in children with 1-6 months old than those in other age groups,the ratio of which were 50 strains and 41 strains,respectively (Pa0.05).The resistant rate of ESBLs-producing strains to penicillins,cephalosporins,quinolones,aminoglycosides and sulfamido was higher than that of non ESBLs-producing strains respectively.And the resistant rates to beta-lactam antibiotics of ESBLs strains were located on a high level.Whether producing ESBLs or not,the 2 bacterias were still sensitive to amikacin,cefoxitin,cefoperazone/sulbactam and imipenem.Conclusions The prevalences of ESBLs-producing escherichia and klebsiella pneumonia were high.There was a multi-drug resistance to the varied antibiotics.It is very important to make sputum culture and use sensitive antibiotics in treatment according to drug sensitivity test to control the occurrence and conveying of the ESBLs.

Objective To apply the Intelligent Device for Energy Expenditure and Activity(IDEEA)system to evaluate the locomotion of patients with protrusion of intervertebral disc(PID)quantitatively and dynamically.Methods 8 patients with PID and 9 volunteers as controls performed a series of daily activities in their nature environment.They were monitored with IDEEA.Results The velocity of flexion forward,time flexion forward,time stand up,time sit down of the patients were significant different to the controls(P<0.05),and the walking speed of the former was slower(P<0.05)than the latter's.Conclusion Physical performance of the PID patients was significantly reduced.The IDEEA can be a good tool to evaluate their ability of locomotion in their daily living.