1.Differential expression of alpha-adrenoceptor subtypes in rat dorsal root ganglion after chronic constriction injury.
Hong-Ju, CHENG ; Ke-Tao, MA ; Li, LI ; Lei, ZHAO ; Yang, WANG ; Jun-Qiang, SI
Journal of Huazhong University of Science and Technology (Medical Sciences) 2014;34(3):322-9
mRNAs of alpha-adrenoceptor (α-AR) subtypes are found in neurons in dorsal root ganglion (DRG) and change after peripheral nerve injury. In this study, the distribution of α-AR subtype proteins was studied in L5 DRG of normal rats and rats with chronic constriction injury of sciatic nerve (CCI). Using immunofluorescence technique, it was found that α1A-, α1B-, and α2A-AR proteins were expressed in large, medium, and small size neurons in normal DRG, and significantly increased in all size neurons 14 days after CCI. α1D- and α2C-AR was also expressed in all size neurons in normal DRG. However, α1D-AR was significantly increased and α2C-AR was decreased in small size neurons 14 days post CCI. α2B-AR neurons were not detectable in normal and CCI DRG. Co-expression of α1A- and α2A-AR in the same neuron was observed in normal DRG and increased post CCI. Collectively, these results indicated that there is distinct distribution of α-AR subtypes in DRG neurons, and the distribution and levels of expression of α-AR subtypes change differently after CCI. The up-regulation of α-AR subtypes in DRG neurons may play an important role in the process of generating and transmitting neuropathic pain.
2.Activation and Apoptosis of Peripheral Blood Lymphocytes in Children with Henoch - Schonlein Purpura and Effects of Triptolide on Them
wei, GUO ; si-guang, LU ; feng-jun, GUAN ; tong-qiang, LIU
Journal of Applied Clinical Pediatrics 1992;0(05):-
Objective To explore the activation and apoptosis of peripheral blood lymphocytes(PBLs) in children with Henoch-Schonlein purpura(HSP) and the effects of triptoIide(TP) on them. Methods The changes of activation and apoptosis were observed on cultured PBLs in children with HSP and healthy controls ,and the effects of TP were compared respectively. Expression of CD3, CD25 and apoptosis rate of PBLs were assayed with flow cytometry. Results The percentage of CD3+ CD25+ cell was significantly higher (P
3.Diagnosis of synovial sarcoma by fine-needle aspiration biopsy and interphase FISH: report of a case.
Sheng-jun XIAO ; Xiao-ling ZHANG ; Ming-qiang ZENG ; Si-en ZENG ; Hong-tao YE
Chinese Journal of Pathology 2010;39(4):278-279
Adolescent
;
Biopsy, Fine-Needle
;
Chromosomes, Human, Pair 18
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Female
;
Humans
;
In Situ Hybridization, Fluorescence
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Oncogene Proteins, Fusion
;
genetics
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Proto-Oncogene Proteins c-bcl-2
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metabolism
;
Sarcoma, Synovial
;
genetics
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metabolism
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pathology
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Soft Tissue Neoplasms
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genetics
;
metabolism
;
pathology
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Translocation, Genetic
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Vimentin
;
metabolism
4.Comparing the effect of different anesthetic inductions in pediatric patients undergoing bronchial foreign body removal
Chunshan DONG ; Qiang LU ; Jun ZHANG ; Yao LU ; Junma YU ; Si CHENG
The Journal of Clinical Anesthesiology 2014;(12):1181-1183
Objective Comparing the effect of different anesthetic inductions in pediatric pa-tients undergoing bronchial foreign body removal.Methods Thirty pediatric patients,aged 9-58 months,undergoing emergency bronchial foreign body removal,were randomly into 3 groups (n=10 each):group sevoflurane (group S),group propofol (group P),and group ketamine (group K).Pa-tients in group S were inducted with sevoflurane 8% inhalation,group P with propofol 2.5 mg/kg in-travenous injection,group K with ketamine 5 mg/kg intramuscular injection.Three groups of pa-tients breathed spontaneously during operative period and received topical anesthesia of lidocaine be-fore the placement of rigid bronchoscopy.Combination of intravenous target-controlled infusion of propofol (target plasma concentration of 3-3.5 μg/ml)and remifentanil (target plasma concentration of 2-3 ng/ml)was used for maintenance of anesthesia.The rigid bronchoscopy was inserted after pre-oxygenation for 3 min.Rigid bronchoscopy was performed and the placement time,the first place-ment successfully rate,hypoxemia and side effects as well as postoperative awaking time were recor-ded.Results The first placement successfully rate,group S 90%,group P 70%,group K 40%,with significant difference among three groups (P<0.05).The incidence of side effects were not signifi-cant difference in three groups;In group S and group P,the placement time and the anesthesia awa-king time was significant shorter than that in group K (P<0.05).Conclusion Compared with propo-fol intravenous induction and ketamine intramuscular induction,the high concentration sevoflurane in-duction can provide faster induction,shorter waking time,and reduceside effects in childen undergo-ing bronchial foreign body removal.
5.Exploration and practice of bilingual teaching in pediatrics of the undergraduates
Bao-Qiang YUAN ; Yuan-Yuan DAI ; Qiu-Ping FAN ; Hua CHENG ; Jun WANG ; Si-Guang LU ;
Chinese Journal of Medical Education Research 2006;0(07):-
To further explore the result of bilingual teaching in pediatrics,we randomly chose 200 undergraduates of 4 class and released students'questionnaires about bilingual teaching with teaching content before and after class to assess students'understanding of bilingual teaching and analysed appraisal result.We found no significant difference of student score between students accepting bilingual teaching and not accepting the bilingual teaching,but there was difference for English tests and expression level.So we think that students can fully accept the bilingual teaching of pediatrics under the premise with selecting appropriate teaching methods and means.
6.Acetylcholine induces hyperpolarization independent of NO-release in guinea pig spiral modiolar artery.
Li LI ; Lei ZHAO ; Jun-Qiang SI
Chinese Journal of Applied Physiology 2009;25(1):107-111
AIMAcetylcholine(ACh) is a neurotransmitter and a potent vasodilator in many vascular beds. ACh hyperpolarizes the smooth muscle cells(SMCs) of arteries including the cochlear spiral modiolar artery(SMA) via an endothelium-dependent mechanism, but the biochemical and biophysical basis of the hyperpolarization and vasodilation remain unclear and controversial.
METHODSUsing intracellular recording techniques and an in vitro preparation of the SMA, the ionic mechanism of the hyperpolarization and a possible role of nitric oxide(NO) were investigated.
RESULTSWith 5 mmol/L K(+) in the bathing solution and a minimum longitudinal tension, ACh (0.1-10 micromol/L) induced a robust hyperpolarization in low RP cells but caused a depolarization in the high RP cells. The ACh hyperpolarization was fast in onset and offset and the amplitude was concentration-dependent(22 and 30 mV by 1 micromol/L and 10 micromol/L ACh, respectively, n = 7 ). ACh also hyperpolarized the cells that initially had a high resting potential (RP) but were pre-depolarized by Ba(2+) (50-100 micromol/L). The onset time courses of the hyperpolarization were often slower in these cases than those without the presence of Ba(2+) . The ACh-induced hyperpolarization was blocked by atropine (0.1- 1 micromol/L, n = 6) or DAMP (50 -100 nmol/L, n = 6, a selective M3 antagonist) and also by BAPTA-AM (10 micromol/L, n = 7, a membrane-permeable Ca(2+)-chelator), or charybdotoxin plus apamin (50-100 nmol/L, n= 4, Ca(2+) -activated K(+) -channel blockers), but not by Nomega-nitro-L-arginine methyl ester (L-NAME, 300 micromol/L, n = 8, an inhibitor of NO-synthase), glipizide (10 micromol/L, n = 4, ATP-sensitive K(+) -channel blocker) and indomethacin (10 micromol/L, n = 4, cyclo-oxygenase inhibitor).
CONCLUSIONIt is concluded that ACh-induced hyperpolarization in the arterial SMCs is primarily due to an activation of calcium-activated potassium channels via M3 receptors of endothelial cell and is independent of NO-release in the spiral modiolar artery.
Acetylcholine ; physiology ; Animals ; Arteries ; Cell Polarity ; physiology ; Cochlea ; blood supply ; physiology ; Guinea Pigs ; Membrane Potentials ; physiology ; Muscle, Smooth, Vascular ; metabolism ; physiology ; Nitric Oxide ; metabolism ; Potassium Channels, Calcium-Activated ; metabolism ; Receptor, Muscarinic M3 ; metabolism
7.Effect of peroxisome proliferators-activated receptor ? ligands on cytotrophoblast invasion in first trimester pregnancy
Shu-Juan LI ; Tao SHANG ; Zi-Qiang CHANG ; Jun LI ; Si-Yang LI ; Qiu-Ling LI ; Guang-Hai RUI ;
Chinese Journal of Obstetrics and Gynecology 2001;0(08):-
Objective To investigate the expression of peroxisome proliferators-activated receptor ? (PPAR?) in trophoblast and relation between PPAR? ligands and trophoblast invasion.Methods We examined the expression of PPAR? by immunohistochemistry,immunocytochemistry and real time quantitative PCR.We next examined,using the cytotrophoblast culture model,the biological role of PPAR? ligands in vitro.Results PPAR? was mainly localized in the nuclei of villous cytotrophoblast and extravillous cytotrophoblast of cell islands and cell columns.In villous tissue and cultured trophoblast from early first trimester,the level of expression of PPAR? mRNA and protein was 36.0?5.1,13.4?3.1 and 1.35?0.08,1.13?0.11;from late first trimester it was 23.3?5.5,6.1?1.3 and 1.17?0.03,0.86 ?0.05,and the expression of PPAR? was obviously decreased (P
8.Differential expression of alpha-adrenoceptor subtypes in rat dorsal root ganglion after chronic constriction injury.
Hong-ju CHENG ; Ke-tao MA ; Li LI ; Lei ZHAO ; Yang WANG ; Jun-qiang SI
Journal of Huazhong University of Science and Technology (Medical Sciences) 2014;34(3):322-329
mRNAs of alpha-adrenoceptor (α-AR) subtypes are found in neurons in dorsal root ganglion (DRG) and change after peripheral nerve injury. In this study, the distribution of α-AR subtype proteins was studied in L5 DRG of normal rats and rats with chronic constriction injury of sciatic nerve (CCI). Using immunofluorescence technique, it was found that α1A-, α1B-, and α2A-AR proteins were expressed in large, medium, and small size neurons in normal DRG, and significantly increased in all size neurons 14 days after CCI. α1D- and α2C-AR was also expressed in all size neurons in normal DRG. However, α1D-AR was significantly increased and α2C-AR was decreased in small size neurons 14 days post CCI. α2B-AR neurons were not detectable in normal and CCI DRG. Co-expression of α1A- and α2A-AR in the same neuron was observed in normal DRG and increased post CCI. Collectively, these results indicated that there is distinct distribution of α-AR subtypes in DRG neurons, and the distribution and levels of expression of α-AR subtypes change differently after CCI. The up-regulation of α-AR subtypes in DRG neurons may play an important role in the process of generating and transmitting neuropathic pain.
Animals
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Cell Size
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Chronic Disease
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Constriction, Pathologic
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Fluorescent Antibody Technique
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Ganglia, Spinal
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metabolism
;
pathology
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Male
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Microscopy, Confocal
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Neurons
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metabolism
;
pathology
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Pain Measurement
;
methods
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Pain Threshold
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Protein Isoforms
;
metabolism
;
Rats
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Rats, Sprague-Dawley
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Receptors, Adrenergic, alpha-1
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metabolism
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Receptors, Adrenergic, alpha-2
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metabolism
;
Sciatic Nerve
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injuries
;
surgery
9.Changes of GABA-activated currents in isolated dorsal root ganglion neurons in rats with neuropathic pain.
He ZHU ; Ke-tao MA ; Li LI ; Zhong-shuang ZHANG ; Jing LI ; Jun-qiang SI
Chinese Journal of Applied Physiology 2011;27(3):376-379
OBJECTIVETo investigate the changes of GABA-activated currents in isolated dorsal root ganglion neurons in rats with neuropathic pain.
METHODSThe neuropathic pain model was established by chronic constriction injury (CCI) 7 days before electrophysiological-recording. The rat DRG neurons were enzymatically dissociated. Whole-cell patch clamp technique was used to record GABA-activated currents. The changes of currents of injured side and opposite side were expected to compare with control group.
RESULTS(1) The currents of injured side of CCI group were notablely decreased compared with control group (GABA concentration, 0.1-1000 micromol/L). (2) By the contrast, opposite side currents of CCI group increased significantly compared with those in injured side and control group (GABA concentration, 0.01-1000 micromol/L).
CONCLUSIONThe data indicates that the chronic constriction injury change both the function of GABAA receptors of injury side and opposite side. The decrease of pre-synaptic inhibition of GABA may be the possible reason of neuropathic pain.
Animals ; Cell Separation ; Constriction ; Ganglia, Spinal ; pathology ; physiopathology ; Male ; Neuralgia ; etiology ; physiopathology ; Neurons ; metabolism ; physiology ; Patch-Clamp Techniques ; Rats ; Rats, Sprague-Dawley ; Receptors, GABA-A ; metabolism ; physiology ; Sciatic Nerve ; injuries
10.Differential effect of calcium-activated potassium and chloride channels on rat basilar artery vasomotion.
Li, LI ; Rui, WANG ; Ke-Tao, MA ; Xin-Zhi, LI ; Chuan-Lin, ZHANG ; Wei-Dong, LIU ; Lei, ZHAO ; Jun-Qiang, SI
Journal of Huazhong University of Science and Technology (Medical Sciences) 2014;34(4):482-90
Spontaneous, rhythmical contractions, or vasomotion, can be recorded from cerebral vessels under both normal physiological and pathophysiological conditions. We investigated the cellular mechanisms underlying vasomotion in the cerebral basilar artery (BA) of Wistar rats. Pressure myograph video microscopy was used to study the changes in cerebral artery vessel diameter. The main results of this study were as follows: (1) The diameters of BA and middle cerebral artery (MCA) were 314.5±15.7 μm (n=15) and 233.3±10.1 μm (n=12) at 10 mmHg working pressure (P<0.05), respectively. Pressure-induced vasomotion occurred in BA (22/28, 78.6%), but not in MCA (4/31, 12.9%) from 0 to 70 mmHg working pressure. As is typical for vasomotion, the contractile phase of the response was more rapid than the relaxation phase; (2) The frequency of vasomotion response and the diameter were gradually increased in BA from 0 to 70 mmHg working pressure. The amplitude of the rhythmic contractions was relatively constant once stable conditions were achieved. The frequency of contractions was variable and the highest value was 16.7±4.7 (n=13) per 10 min at 60 mmHg working pressure; (3) The pressure-induced vasomotion of the isolated BA was attenuated by nifedipine, NFA, 18β-GA, TEA or in Ca(2+)-free medium. Nifedipine, NFA, 18β-GA or Ca(2+)-free medium not only dampened vasomotion, but also kept BA in relaxation state. In contrasts, TEA kept BA in contraction state. These results suggest that the pressure-induced vasomotion of the isolated BA results from an interaction between Ca(2+)-activated Cl(-) channels (CaCCs) currents and K(Ca) currents. We hypothesize that vasomotion of BA depends on the depolarizing of the vascular smooth muscle cells (VSMCs) to activate CaCCs. Depolarization in turn activates voltage-dependent Ca(2+) channels, synchronizing contractions of adjacent cells through influx of extracellular calcium and the flow of calcium through gap junctions. Subsequent calcium-induced calcium release from ryanodine-sensitive stores activates K(Ca) channels and hyperpolarizes VSMCs, which provides a negative feedback loop for regenerating the contractile cycle.