Chronic Disease ; Humans ; Male ; Middle Aged ; Mineral Oil ; adverse effects ; Occupational Exposure ; adverse effects ; Pigmentation Disorders ; chemically induced

Objective:To establish a ?B2 crystallin gene knockout mice model.Methods: The gene-targeting vector was established by the technique of American inGenious Targeting Laboratory.PCR technique was used to identify the genotype of the model mice and expression of ?B2 crystallin protein was detected by Western blot.Results: Three genotypes were successfully(identified) and expression of ?B2 crystallin protein was not detected in gene knockout mice.Conclusion: The ?B2 crystallin gene knockout mice model has been successfully established.

The activity of interleukin-2 (IL-2) and expression of interleukin-2 receptor (IL-2 R) were measured in children with acute glomerulonephritis (AGN) in order to detemine the mechanism of the disease Our results showed that the activity of IL-2 in peripheral blood lymphocytes (PBL) of children with AGN was lower than that tof the control group (P

Objective:To study the expression and significance of c-myc in pediatric lymph node swollen diseases.Methods:Expression of c-myc gene in swollen lymph node specimens of 84 children with lymph node swollen diseases were determined with SP immunohistochemistry technique.Results:(1)The positivity rates of the c-myc gene of lymphadenitis,reactive hyperplasia (RH) and lymphoma were 15.62%,40.48%,and 80.00%,respectively,and the difference was significant( ? 2=14.59, P

OBJECTIVE:To discuss the role of clinical pharmacists in providing pharmaceutical care in respiratory department.METHODS:The clinical pharmaceutical care provided by clinical pharmacists in respiratory department was analyzed and summarized.RESULTS & CONCLUSION:The pharmaceutical care carried out in respiratory department can help reduce the incidence of medication errors.

OBJECTIVE:To discuss the position and role of hospital in pharmacovigilance.METHODS:The knowledge related to pharmacovigilance was introduced and the status quo and the responsibility assumed by hospital and its stuff in the pharmacovigilance work were analyzed.RESULTS & CONCLUSION:Hospital ought to be one of the main part in the pharmacovigilance,and through training to enhance the knowledge of the doctors,pharmacists and nurses about the pharmacovigilance,so that could build good system for the safety monitoring of drugs,and also could establish rules of pharmacovigilance to reduce ADR,improve rational use of medicine.

BACKGROUND: Mild hypothermia can protect cerebral cell from injury induced by ischemia, however, whether it can protect cerebral function after cerebral resuscitation or not?OBJECTIVE: To investigate the differences between neural functional outcome and living self-care ability of patients after 3-month cerebral resuscitation with mild hypothermia technique compared with normal temperature technique.DESIGN: Randomized control study.SETTING: Emergency Center of Gulou Hospital Affiliated to Medical College of Nanjing University.PARTICIPANTS: Totally 16 patients rescued with cardiopulmonary resuscitation were selected from Gulou Hospital of Nanjing between February and October 2002. All patients had systemic lesion and intoxication, and were treated with cardiopulmonary resuscitation because of heartbeat and respiratory arrest. There were 10 males and 6 females aged 27-57 years.According to the application of mild hypothermia or not, 16 cases were divided randomly into normal temperature group and mild hypothermia group with 8 in each group.METHODS: After cardio-pulmonary resuscitation, patients in mild hypothermia group were treated with medicine + caput ice cap + electronic ice blanket method to reduce the temperature. The temperature of brain was kept at 32 to 34 ℃ for 72 hours. Patients in normal temperature group were treated at the room temperature.MAIN OUTCOME MEASURES: ① Blood-oxygen saturation, blood pH value, intracranial pressure, mean arterial pressure and Glascow Coma Scale of patients were measured on the third and seventh day after resuscitation. ② After 3-month resuscitation, Glascow Coma Scale was followed:85-100 points as functional independency, 74-84 as moderate disability,10-50 as severe disability and ＜ 10 as vegetative slate; meanwhile, living self-care ability was measured with Bathel index of 10 items in total. Every item was scored from 0 to 15 points with 100 in total, and ＜ 60 was determined as unable self-care.RESULTS: Totally 16 patients entered the final analysis. ① On the third day, mean cerebral temperature and intracranial pressure in the mild hypothermia group were lower than those in the normal temperature group [(33.1±0.26)℃, (37.4±18)℃; 15.7 mm Hg, 19.1 mm Hg, P=0.027, 0.043]. The two groups were similar in the Score of blood-oxygen saturation, blood pH value, mean arterial pressure and Glascow Coma Scale (P ＞ 0.05). ② On the seventh day, Glascow Coma Scale in the mild hypothermia group was higher than that in the normal temperature group [(9.1±1.4), (11.2±1.6) points,P=0.032]. ③ After 3 months, Glascow Coma Scale and Barthel index in the mild hypothermia group were higher than those in the normal temperature group [(12.9±1.75), (9.9±1.9) points; (86±6), (52±12) points, P ＜ 0.05-0.01].CONCLUSION: Mild hypothermia can decrease intracranial pressure during the early cerebral resuscitation, is especially effective in the recovery of long-term neural function and activity of daily living, and has remarkable protecting effect on cerebral function.

Ech1 is the key enzyme of fatty acid β-oxidation metabolism,and mainly locates in peroxisome and mitochondria.Recent studies indicate that abnormal expression of Ech1 is related with liver carcinoma,stomach carcinoma and chronic lymphoid leukemia,but the mechanism is not very clear.Other members of Echfamily are also closely related to many diseases.

Diagnosis, Differential ; Humans ; Pneumoconiosis ; diagnosis

Objective To explore the effect and mechanism of montelukast sodium on transforming growth factorβ1 (TGF -β1 )expression in children with asthma.Methods 80 children with asthma were divided into 2 groups.The control group was treated with common medicine,and the observation group was treated with monte-lukast sodium.The clinical effect of two groups was evaluated.The peripheral eosinophile granulocyte(EOS)count of children was measured before and after treatment.Double antibody sandwich enzyme -linked immunosorbent assay (DAS -ELISA)was used to detect the serum levels of TGF -β1 and leukotriene E4 (LTE4)before and after treatment.Results The total effective rate of montelukast sodium group was 95.0%,remarkable effect in 28 cases, 10 cases were effective,and 2 cases failed.The total effective rate of the control group was 82.5%,23 cases had remarkable effect,effective in 10 cases,ineffective in 7 cases.The effective rate of the montelukast sodium group was statistically higher than the control group(χ2 =4.200,P <0.05).After treatment,the peripheral eosinophil count of patients of two groups significantly decreased than before treatment (control group:χ2 =3.251,P <0.05;observation group:χ2 =3.214,P <0.05).There was significant difference between the two groups(χ2 =3.254,P <0.05).Before treatment,serum levels of TGF -1 showed no statistical difference between the two groups (P >0.05).After treat-ment,the levels of serum TGF -1,LTE4 of the observation group decreased more remarkably when compared with the control group (TGF -β1:t =2.06,P <0.05;LTE4:t =2.14,P <0.05).Conclusion Montelukast sodium has good clinical effect on childhood asthma.It can inhibit the expression of TGF -1 in childhood asthma,and its mechanism may be related to eosinophils and the level of LTE4.