Objective:To study three-dimensional hepatic microcirculation in normal rabbits.Methods:The corrosion casts was produced using methyl methacrylate and the HA,PV and BD were observed by scanning electron microscopy(SEM).Results:The diameter range of hepatic sinus was about 9.5～10.9?m,the ratio of HA/PV,HA/BD and PV/BD were about 0.28?0.03,0.26?0.06 and 0.89?0.12.The ratio of hepatic sinus and cells was about 31.87%.There was no significant rheological respectively between entering and outflowing liver blood.Conclusion:It is an effective method to study the hepatic microcirculation by using both methylmethacrylate and scanning electron microscopy.The results can serve as contrast standard for hepatic pathological research.

Objective:Observe the curative effects of transcutaneous electrical stimulation (TES) combined with medicine when it is used to treat the multi-infarct dementia (MID). Method: 54 dementias are randomly divided into two groups: The group TES and the matched group, with each 27 patients, in which, the group TES adopts TES with medicine and the matched group is only supplied to medicine. Score the MMSE, MBI and HDS before and after treatment. Judge the curative effects according to the scores of MBI. Results: In comparison, the MMSE, MBI and HDS are greatly different, the curative efficiency of the group tES is higher than the one of the matched group and the difference is significance in statistic(P

Objective:To develop a novel amperometic immunosensor for the determination of Urinary Trypsinogen-2(TSG-Ⅱ).Methods:the immunosensor was developed by modifying Nafion film on the glassy carbon electrode(GCE),then thionine and colloidal gold were immobilized by static electricity and covalent bonding self-assembly.And then the antibody of Urinary TSG-Ⅱwas absorbed on the surface of nano-gold monolayer.Finally,Horseradish peroxidase(HRP) blocked the non-specific adsorption site on the electrode.Result:Experiment results showed that the good linear relations between peak current and concentration of TSG-Ⅱ were established in the range of 6.0-100?g/L.The detection limit was 3.5?g/L.Conclusion:The amperometie immunoassay biosensor has high sensitivity and good specificity and it has laid the foundation to establish a new method for detection of TSG-Ⅱ.

3.5cm in diameter were given multiple overlapping ablations that encompassed the entire les- ions.The operation was performed under local anesthesia in association with systemic analgesia. Results:The procedure was successfully accomplished in all the 16 patients. Adverse reactions happened at 2 ～5 postoperative days,including local pain and discomfort,pyrexia,and elevations of ALT levels. No bile leakage or hemorrhage occured.Follow-up observations in 16 patients for 1～3 years (mean,1 years and 8 months)showed a complete response (CR)in 15 patients and a partial response (PR)in l patient by one mission of RFA,the effective rate (CR+PR)being 100%.The hepatic hemangiomas decreased by a mean of 62.0%(range,50.1%～84.7%)in diameter. Conclusion:RFA is a safe and effective therapy for liver hemangiomas in properly selected patients by experienced hands.

Platelet-derived growth factor(PDGF), as an important cytokine, can induce mesangial cell proliferation and accumulate extracellular matrix. The proliferation of the mesangial cells correlate with the cell cycle regulation. The different isoform of PDGF works on mesangial cells to produce different effects. This review summarizes the relevant mechanism about PDGF inducing proliferation of mesangial cells.

Objective To explore the expression and clinical significance of matrix metalloproteinase-9 (MMP-9) in gastric cancer tissues.Methods The expressive level of MMP-9 in normal gastric mucosa tissues,para-carcinoma tissues,and cancer tissues were determined with histochemistry streptavidin-perosidase (SP) methods.The relationship of MMP-9 and gastric cancer tissue were analyzed.Results The positive expression rate of MMP-9 in the gastric cancer tissues was significantly higher than that in normal gastric mucosa tissues and para-carcinoma tissues (x2 =86.66,P ＜0.01).The positive expression rate of MMP-9 in gastric cancer tissues was related to differentiated degree of cancer tissues,tumor node metastasis (TNM) stage,infiltration degree,and lymphatic metastasis (x2 =7.76,P ＜ 0.05; x2 =12.94,54.37,11.16,P ＜ 0.01).Conclusions MMP-9 probably participates in the occurrence and development of a gastric cancer,and is related to its invasion and metastasis.

Objective To study the effects of protein kinase C (PKC) inhibitor staurosporine (STS) on the proliferation and apoptosis of human cholangiocarcinoma QBC-939 cells and to explore its possible mechanism. Methods CCK-8 was used to detect the effects of PKC inhibitor STS on the proliferation of human cholangiocarcinoma QBC-939 cells. The effects of STS on the ultrastructural characteristics of QBC-939 cells were observed by routine transmission electron microscopy (TEM). The apoptosis rate and the cell cycle distribution of QBC-939 cells were detected by flow cytometry. The expression of cyclin B1,Cdk1 and p-Cdk1 in QBC-939 cells was detected by Western blotting. Results STS could significantly inhibit the proliferation of QBC-939 cells in a dose-dependent manner (P <0. 05) and the half inhibitory concentration ( IC50 ) of QBC-939 cells at 24th and 48th h was 334 nmol·L-1 and 118 nmol · L-1 , respectively. TEM observed that STS could induce typical apoptotic bodies and super-microstructural changes of QBC-939 cells. By Annexin V-FITC/ PI double labeling flow cytometry,we found that the apoptotic rate of QBC-939 cells after treatment with STS for 0,12,24 and 48 h was (10. 16±4. 52)% ,(22. 35±2. 19)% ,(34. 27±2. 30)% and (59. 70±5. 97)% ,respectively. By flow cytometry,compared with the control group,STS could significantly increase apoptosis rate of QBC-939 cells,decrease the percentage of cells in G0 / G1 phase and increase the percentage of cells in G2 / M phase (P<0. 05). Western blotting proved that the expression levels of cyclin B1 and Cdk1 proteins in the STS-treated QBC-939 cells were significantly decreased (P<0. 05),while the expression level of p-Cdk1 protein in the STS-treated QBC-939 cells was significantly increased ( P < 0. 05 ). Conclusion STS can significantly inhibit cell proliferation and induce apoptosis of human cholangiocarcinoma QBC-939 cells and the mechanism may be related to cell cycle arrest at G2 / M phase.

Objective To analyze and solve the difficulties in rapidly expanding the mobile field hospital in earthquake relief mission.Methods Aimed at the difficulties in the speed,advance men were sent out before the expansion.Then the personnel were regrouped in the proceeding of the expansion,and the vehicles entered the district according to the predefined area and order.Results The expanding speed was raised.Conclusion The scheme meets the requirements of the first aid in the mobile field hospital.

OBJECTIVE To study the safe methods of used disposal medical supplies to provide scientific results of decision-making for the health administration. METHODS Using the methods of "standard prevention", "isolated transformation", and "unit calculation", the disposable medical supplies were safely treated in a completely-closed and entirely-programmed way without disinfecting or damaging. The efficacy of the methods was evaluated. RESULTS Form 2006 to 2007, the distribution of disposable medical supplies was 13 306.12 kg and 14 046.00kg and the retrieved quantity was 13 306.00 kg and 14 041.00kg. The improved methods of quantification solved the problem existing in distribution and retrieving of the disposable medical supplies. The safety and convenience of the whole process had been greatly improved. CONCLUSIONS Compared with destroying shapes, disinfecting and "face to face" exchange methods, the methods of using "standard prevention", "isolated transformation" and "unit calculation" are safer, more economic, and more effective.