PFTK1,an important member of cyclin dependent kinase Cdc2 family,is over expressed in many malignancies.PFTK1 involves in multiple processes,such as regulation of cell cycle,tumor invasion and metastasis,and the Wnt signal transduction pathway,which has a significant impact on prognosis of tumor.

Objective To investigate the effect of 8-Nitrochyrsin on cell proliferation and cell apoptosis of HL-60 cells in vitro.Methods 8-Nitrochyrsin on proliferation of HL-60 cells was detected by MTT assay;The trypan blue exclusion method was used to count the number of viable and dead cells,then the survival rate and growth curve were achieved;DNA ladder bands were observed by DNA agarose gel electrophoresis;HL-60 cell apoptotic percentage was detected by flow cytometry with PI staining.Results 8-Nitrochrysin inhibited proliferation of HL-60 cells in a concentration-dependent manner;8-Nitrochyrsin possess the inhibitory effect on the proliferation and growth of HL-60 cells;8-Nitrochrysin possess the significantly effect of inducing apoptosis of HL-60 cells.Conclusion 8-Nitrochyrsin possess the inhibitory effect of the proliferation and growth of HL-60 cells and significantly induces apoptosis of HL-60 cells.

Recently, stem cell like side population (SP) cells have been found in many normal organizations and malig?nant tumors, which have the proficiency of differentiation and self-renewal. These cells play an important role in cancer stem cell research, though they occupy a low proportion in total cells. Here, we reviewed the foundation of SP cells, and their rela?tionship with stem cells, and the clinical application in the future.

Humans ; Infection Control ; Root Canal Therapy

Objective To understand the common complications of PICC in olinical use. Methods Retrospectively summarize the data of PICC used in patients of SICU in 1999. Results In 1999, there were 14 pateints using PICC, among them 12 patients were male and the others were female. The average age was 71 years old. Embolus 4 cases' PICC were pulled out. Phlebitis occured in only one case. There are 2 patients presenting hyperpyrexia pulled the PICC out. But the cultures of PICC were negative. Conclusions The common complications of PICC are embolism,phlebitis and infection.

Objective: To study the chemical constituents in Vitis thunbergii var. taiwaniana specially grown in Taiwan, China. Methods: The compounds were isolated by repeated HP20 macroporous adsorption resin column combined with Sephadex LH-20, ODS, and silica gel column chromatography. Their structures were identified on the basis of extensive spectroscopic data analysis and by comparison of their spectral data reported. Results: Twelve compounds were identified as resveratrol (1), trans-ε-viniferin (2), (7R, 8R)-threo-4, 7, 9, 9'-tetrahydroxy-3, 3'-dimethoxy-8-O-4'-neolignan 7-O-β-D-glucopyranoside (3), (7S, 8R)-urolignoside (4), schizandriside (5), vitisin A (6), vitisin B (7), davidiol A (8), 3, 5, 4'-trihydroxystilbene 4'-O-β-D-glucopyranoside (9), ampelopsin C (10), (7R, 8S)-dihydrodehydrodiconiferyl alcohol 9-O-β-D-glucopyranoside (11), and epicatechin (12). Conclusion: Compounds 3-5 are separated from the plants of Vitis L. for the first time, and compounds 8, 9, 11, and 12 are separated from V. thunbergii var. taiwaniana for the first time.

Objective To investigate the direct effects of midazolam on vascular tension and the possible mechanism Methods Male Wistar rats weighing 200 250g were decapitated and the thoraco abdominal sect of aorta was removed and placed in modified Kreb's solution balanced with 95% O 2 and 5% CO 2 After removal of surrounding connective tissue and fat, the aorta was cut into rings 3 4mm in length The endothelium of some of the aorta rings were denuded Aorta rings were suspended in a 37℃ bath of Kreb's solution which was continuously balanced with 95% O 2 and 5% CO 2 One end of the ring was fixed and the other end was connected to a tension monitor through a tension transducer The intactness of endothelium was tested and confirmed by addition of phenylephrine(PE) first and then acetylcholine Aorta rings of each animal were divided into four groups: endothelium intact control group(Ⅰ); endothelium intact experiment group(Ⅱ);endothelium denuded control group(Ⅲ);and endothelium denuded experiment group(Ⅳ) The experiment was carried out in two parts: Part Ⅰ: the effect of midazolam (3?10 -6 ,10?10 -6 ,30?10 -6 ,100?10 -6 mol/L) on endothelium intact and denuded aorta rings which were precontracted with PE(10 -6 mol/L); part Ⅱ: before different concentrations of midazolam was added, the endothelium intact rings were pretreated with methylene blue(10 -5 mol/L) and the endothelium denuded rings were pretreated with verapamil(5?10 -6 mol/L) or teraethylammonium(TEA,5?10 -3 mol/L).Results The four concentrations of midazolam examined produced relaxation in both endothelium intact and denuded rings Relaxation of endothelium denuded rings was less than that of endothelium intact rings (P0 05) When pretreated with TEA, midazolam produced greater relaxation in endothelium denuded rings(P

Objective To evaluate clinical application of arterial embolotherapy on hyperthyroidism. Methods 11 patients with hyperthyroidism were performed with thyroid superior and inferior arterial super selective arteriography and interventional embolization by polyvinyl alcohol(PVA),gelfoam particles and wool gianturco coil. Results The procedures were succeeded in 11 patients. After the embolotherapy , the thyroid function gradually returned to normal level in 10 patients. The symptom was not controlled satisfactorily in 1 patient, who underwent the right thyroid inferior arteries superselective arteriography and interventional embolization, and then the thyroid function gradually returned to normal level. No serious complications occurred in all patients. Conclusions Arterial embolotherapy is a safe, simple,symptomless, reliable and very effective for treatment of hyperthroidism.

To improve and optimize marine antimicrobial peptide R-1 production by a newly isolated Brevibacillus laterosporus Lh-1, Plackett-Burman (PB) design and response surface methodology (RSM) using central composite design was adopted in culture conditions. MINITAB 15.0 was used for planning the experiments, data analysis, contour diagrams and response optimizations. In this study, PB design was undertaken to evaluate the effects of the fifteen factors. By the statistical regression analysis, the significant factors affecting the novel antimicrobial peptide R-1 in submerged fermentation by Br. laterosporus Lh-1 were determined as follows: glucose, peptone and CaCl2. Then a RSM was used to optimize the above critical internal factors, and the optical concentration of the variables were deter-mined as: 15.72 g/L glucose, 6.01 g/L peptone and 3.29 g/L CaCl2. The content of R-1 was increased from 82.15 kU/mL to 116.27 kU/mL.