Silence information regulator factor 1 (Sirt1) has been demonstrated to be a multi-functional regulator act as a deacetylase.It regulates in critical pathophysiologic pathways,including gene transcription,energy metabolism and senescence.It has been shown that Sirtl regulates the growth and development of myocardial tissue and the formation of blood vessels,also modulates the energy metabolism and oxidative stress of cardiovascular system and so forth.The transcriptional regulator may play a potential role in clinical application and research of cardiovascular diseases.

Objective To clone 3-dehydroquinate synthase cDNA from Echinacea angustifolia and investigate its tissue expression characteristics in various tissues.Methods By using homology cloning and RACE-PCR,the cDNA encoding 3-dehydroquinate synthase was amplified with cDNA library of cultured plantlets as the template.The specificity expression profile in different tissues including roots,stems, leaves,and flowers of E.angustifolia was investigated by semi-quantitative RT-PCR as well.Results The full length cDNA of 3-dehydroquinate synthase(named as EanaroB) had 1 424 bp with an open reading frame encoding 442 amino acids of protein and its EanaroB was around 80%homologous with the sequences of 3-dehydroquinate synthase from other plants.The sequence was reported to the GeneBank and coded as EU293857.The results of semi-quantitative RT-PCR indicated that the expression of EanaroB was detected in different tissues,while only the expression in leaves and flowers reached to a high level. Conclusion The cDNA encoding EU293857 from E.angustifolia is cloned and reported.This work underlays the first step for exploring the pathway of caffeic acid derivatives biosynthesis and improving the content of caffeic acid derivatives including phenylethanoid glycosides.

Fibrosis ; Humans ; Inflammation ; MicroRNAs ; Neoplasms

Objective To compare the efficacy of 10-day sequential therapy (including rabeprazole amoxillin clarithromycin tinidazole) and 7-day traditional trigeminy therapy (including rabeprazole amoxillin and clarithromycin) in patients with duodenobulbar ulcer and Hp infection. Methods Ninty-five patients with duodenobulbar ulcer and Hp infection were enrolled into the study and divided into two groups randomly:sequential therapy group and traditional trigeminy therapy group. Patients in the first group received 10-day sequential medications:rabeprazole 10 mg plus amoxillin 1 g for the first 5 days,followed by rabeprazole 10 mg plus clarithromycin 500 mg and tinidazole 500 mg for another 5 days;while in the second group patients received the standard 7-day traditional triple medications:rabeprazole 10 mg plus clarithromycin 500 mg and tinidazole 400 mg. All drugs were given twice daily. All patients received rabeprazole 10 mg daily following the two types of therapys for another four weeks. Hp statuses were assessed by rapid urease test and 14C urea breath test at baseline and 4 weeks after completion of the treatment. Ulcer cicatrization was assessed by gastroscope. Results The eradication rate of Hp infection was significantly higher in sequential group than triple group (89.5%vs. 70. 2%, P ＜ 0. 05 ), but we found no statistically significant difference in the comparison of the ulcer cicatrization rate between sequential group and triple group ( 87.5% vs. 82. 9%, P ＞ 0. 05 ). Conclusion Sequential therapy had a better Hp eradication effect than standard triple therapy, but the eradication rate was very close between the two therapys.

Objective: To investigate the antitumor effect of SGI-1776 on human ovarian cancer HO-8910 cells and its molecular mechanism. Methods: HO-8910 cells were cultured in vitro, and the proliferation inhibitory effects of SGI-1776 were determined by MTT assay and colony formation assay. The effect of SGI-1776 on the distribution of cell cycle phase was observed by flow cytometry with propidium iodide (PI) staining. hTe inhibition rate of migration and invasion were valued by transwell cell assay. Multiple molecular techniques, such as ELISA, Western blot, siRNA and cDNA transfection were used to explore the molecular mechanism. Results: SGI-1776 presented dramatic anti-tumor activity against HO-8910 cells in vitro, inhibited the cells proliferation and colony formation, and attenuated the migration and invasion in a dose-dependent manner, accompanied by cell cycle arrest in G1 phase. SGI-1776 caused the proliferation inhibition with concomitant decrease in Pim-1 kinase activity, down-regulated the expression of Pim-1 protein and and its downstream genes, such as CDK6, pCDK6, CDK4, pCDK4, CDK2 and pCDK2, and increased the expression of P21 and P27. Down-regulation expression of Pim-1 by siRNA followed SGI-1776 treatment resulted in enhanced cell proliferation inhibition rate and attenuated migration/invasion. Up-regulation of Pim-1 by cDNA transfection attenuated SGI-1776-induced cell proliferation inhibition and its migration/invasion. Conclusion: Pim-1 mediates the biological effect of SGI-1776 in human ovarian cancer HO-8910 cells, suggesting Pim-1 might be a novel target for human ovarian cancer.

Objective To review the recent studies on the suppressing function of breast cancer metastasis suppressor 1 (BRMS1) in breast cancer metastasis. Methods The recent literatures on the mechanisms of BRMS1 in the breast cancer that were published in and abroad were reviewed and summarized. Results BRMS1, similar to the other anti-metastasis genes, only suppresses the metastasis of breast cancer cells but has nothing to do with the growth of tumor. BRMS1 could suppress metastasis of tumor cells by reestablishing both the homospecific and the heterospecific gap junctional intercellular comminications (GJIC) and by altering the expressions of relevant metastasis genes in the breast cancer. Conclusion Further studies on BRMS1 may be helpful to understand the metastasis of breast cancer, which may provide a new way for the diagnosis and treatment of breast cancer.

Cerebralischemiacaninducecelnecrosisincoreoftheinfarction,andapoptosisinthe ischemic penumbra. The mitogen-activated protein kinases (MAPKs) signaling pathw ays are activated after cerebral ischemia, causing programmed cel death, including apoptosis. This article review s the relationship betw een the MAPKs signaling family and neuronal apoptosis after cerebral ischemia.

Objective To study the significance of acute pancreatitis AP complications by acid-base distur-bance. Methods The arterial blood gas, serum electrolytes parameter and clinical data of 85 patients were analyzed.Results Acid-base disturbance of different types commonly existed in the early stage of AP patients. There are sig-nificant statistical differences in the indices of PaO2, PaCO2, SaO2 between group MAP and group SAP( P < 0.05 or P < 0.01 ). Conclusion Acid-base disturbance are very common in the AP patients and this-will make the condition worse,they should be treated promptly.

Objective To compare the short-term clinical effect of K-Rod pedicle dynamic fixation system and lumbar fusion for multi-ple segmental lumbar degeneration.Methods Between January 2010 and October 2012,56 patients with segmental lumbar degeneration who were accepted K-Rod pedicle dynamic fixation system and posterior lumbar interbody fusion were retrospectively reviewed.The 56 patients were divided into K-Rod and fusion group,28 patients with K-Rod pedicle dynamic fixation system and 28 cases with traditional lumbar fusion and internal fixation.Clinical assessments were based on comparion of Oswestry disability index score ,visual analogous scale (VAS),im-provement rate of low back pain,postoperative lumbar spine X-ray,average operation time and intraoperative blood loss of two groups.Results All of 56 cases were followed up for 12 to 24 months,the difference of Oswestry disability index score and VAS between two groups were not statistically significant(P >0.05),but the difference of average operation time,blood loss and the average length of stay(ALOS)were statis-tically significant(P <0.05),the K-Rod group was superior to the fusion group.Conclusion In the treatment of multiple segmental lumbar degeneration both K-Rod pedicle dynamic fixation system and traditional lumbar fusion can obtain satisfactory short-term clinical effect,but the K-Rod pedicle dynamic fixation system has advantages of less intraoperative blood loss,shorter operation time,postoperative small effects on lumbar spine mobility.