Objective To clone 3-dehydroquinate synthase cDNA from Echinacea angustifolia and investigate its tissue expression characteristics in various tissues.Methods By using homology cloning and RACE-PCR,the cDNA encoding 3-dehydroquinate synthase was amplified with cDNA library of cultured plantlets as the template.The specificity expression profile in different tissues including roots,stems, leaves,and flowers of E.angustifolia was investigated by semi-quantitative RT-PCR as well.Results The full length cDNA of 3-dehydroquinate synthase(named as EanaroB) had 1 424 bp with an open reading frame encoding 442 amino acids of protein and its EanaroB was around 80%homologous with the sequences of 3-dehydroquinate synthase from other plants.The sequence was reported to the GeneBank and coded as EU293857.The results of semi-quantitative RT-PCR indicated that the expression of EanaroB was detected in different tissues,while only the expression in leaves and flowers reached to a high level. Conclusion The cDNA encoding EU293857 from E.angustifolia is cloned and reported.This work underlays the first step for exploring the pathway of caffeic acid derivatives biosynthesis and improving the content of caffeic acid derivatives including phenylethanoid glycosides.

Silence information regulator factor 1 (Sirt1) has been demonstrated to be a multi-functional regulator act as a deacetylase.It regulates in critical pathophysiologic pathways,including gene transcription,energy metabolism and senescence.It has been shown that Sirtl regulates the growth and development of myocardial tissue and the formation of blood vessels,also modulates the energy metabolism and oxidative stress of cardiovascular system and so forth.The transcriptional regulator may play a potential role in clinical application and research of cardiovascular diseases.

Cholestasis ; complications ; Consensus ; Humans ; Liver Diseases ; diagnosis ; etiology ; therapy ; Practice Guidelines as Topic

Fibrosis ; Humans ; Inflammation ; MicroRNAs ; Neoplasms

Since 2015, US government announced the Precision Medicine Initiative, the concept of“precision medicine” is spreading rapidly, which has seemingly become the mainstream of future medicine.Laboratory medicine based on personalized information brought by the development of precision medicine will be an entirely new area, however its complexity is far beyond the current knowledge and laboratory medical scientists are encouraged to be actively involved in this emerging field.Opportunities and challenges may coexist, which mainly include: screening and evaluation of circulating biomarkers, selection and standardization of appropriate molecular diagnostic techniques, acquisition of high quality biological specimens and establishment of sample library, and comprehensive interpretation of the results in order to achieve and improve individualized precise prevention, diagnosis, and treatment for specific diseases and patients.

Objective: To investigate the problems and improve the quality of experimental teaching for medical laboratory instruments.Method: A questionnaire was made among 100 laboratory medicine students in Chongqing University of Medical Sciences.Result: About eighty-six percent of the students were satisfied with the experimental teaching for medical laboratory instruments,but there were still problems: absence of clear objectives in experimental teaching;shortage of activity of medical laboratory instruments experiment;lack of modern large-scale instruments.Conclusion: the key points to improve experimental teaching quality are to set up clear experimental teaching objective;update instruments for experimental teaching;structure simulator laboratory with modern education simulator technology.

Objective To review the recent studies on the suppressing function of breast cancer metastasis suppressor 1 (BRMS1) in breast cancer metastasis. Methods The recent literatures on the mechanisms of BRMS1 in the breast cancer that were published in and abroad were reviewed and summarized. Results BRMS1, similar to the other anti-metastasis genes, only suppresses the metastasis of breast cancer cells but has nothing to do with the growth of tumor. BRMS1 could suppress metastasis of tumor cells by reestablishing both the homospecific and the heterospecific gap junctional intercellular comminications (GJIC) and by altering the expressions of relevant metastasis genes in the breast cancer. Conclusion Further studies on BRMS1 may be helpful to understand the metastasis of breast cancer, which may provide a new way for the diagnosis and treatment of breast cancer.

Objective To investigate the effects of aerobic exercise training on oxidative stress and ultrastructure of pancreas tissue in diabetic rats and the mechanism of protective effects of aerobic exercise training on pancreatic mitochondria in diabetic rats.Methods Thirty Sprague-Dawley rats were randomly divided into three groups:a normal control group,a diabetic model group and an exercise diabetic group,with 10 rats in each group.Type 2 diabetic rat models were established by intra-abdominal injection of streptozotocin (STZ).The success rate of establishing diabetic rat models was 85.7％.The normal control group and diabetic model group were not given exercise training.Exercise group was trained with 8 weeks of swimming of medium strength,eg.60 min/time,5 d/week. After training for 8 weeks, the activities of superoxide dismutase(SOD), glutathion peroxidase (GPX),nitric oxide synthase (NOS) and the contents of malondialdehyde (MDA) and nitric oxide (NO) in pancreatic mitochondria were observed respectively.The ultrastructures of pancreas tissue in rats were observed under electron microscope.Results After swimming training for 8 weeks,the activities of SOD and GPx in pancreatic mitochondria in exercise diabetic group elevated more obviously than those in diabetic model group ( P ＜ 0.05 ),while the activity of NOS and the contents of MDA and NO reduced obviously (P ＜ 0.05 ).Compared with diabetic model group,the ultrastructures of pancreas tissue in exercise diabetic group improved,and the histomorphology was similar to normal control group.Conclusions Aerobic exercise training can provide protective effects on pancreas in diabetic rats,and can enhance antioxidation ability to eliminate free radicals,improve the ultrastructures of pancreas tissue.

Objective To Study the correlation factors for diabetes pathogenesis of Uygur Nationality in Urumuqi.Methods A cluster sampling was done in 1002 Uygur people aged 25 to 74 years in a part of working units and living area in Urumuqi.All the people received 75g oral glucose tolerance test.Waist and hip circumference,blood pressure,height and body weight were measured.A questionnaire involved in the family history,diet,physical activity and the expenditure on diet was taken.Results(1)63% of the people was male,while 37% was female in our study.The constituent ratio of age was as following:25y～,23.08%;35y～,36.90%;45y～,19.64%;55y～,14.86%and 65y～,5.51%.(2)The prevalence of DM in the study was 14.69%,the abnormality of glucose metabolism was 29.89%.(3)The correlation factors of diabetes were family history of diabetes(OR 0.59),age(OR 0.83),high blood pressure(OR 0.912),waist-to-hip ratio(OR 3.851)and financial spending on diet(OR 5.279) in logistic regression analysis.Conclusions Diabetes-correlated factors are high financial spending on food especially meat products and little cellulose intake,high waist-to-hip ratio.To decrease the prevalence rate of diabetes in Uygur nationality,it is most important to change the diet habit and decrease intra-abdominal fat deposition