Many previous studies have reported that static stretching (SS) may decrease muscle stiffness and compromise muscles’ ability to produce maximal strength. However, the effects of SS at different repetition durations and numbers within a constant total time remain unclear. Therefore, the purpose of this study was to examine whether SS for a constant total time (2 min) with different repetition durations and numbers (e.g., 60 s × 2 times, 30 s × 4 times, and 10 s × 12 times) produces different changes in muscle stiffness and strength. Fifteen healthy males (mean age: 23.3 ± 1.0 years) participated in this study. Muscle stiffness was measured during passive ankle dorsiflexion using dynamometer and ultrasonography. In addition, muscle strength of the plantar flexors was measured using a dynamometer at 0° of plantarflexion with the hip and knee joints fully extended. Muscle stiffness and strength were measured before and immediately after SS. Each experimental protocol was conducted in random order with at least a 1-week interval but no longer than a 2-week interval between testing sessions. The results showed that there were no significant interaction effects on muscle stiffness and strength. However, in all experimental protocols, muscle stiffness and strength immediately decreased after SS. In conclusion, SS for a constant total of 2 min decreases muscle stiffness and strength regardless of repetition durations and numbers of each individual SS.