Objective To study the effect of atorvastatin on the levels of high sensitive C-reactive protein (hs-CRP)in coronary heart disease(CHD)patients with different Gensini score.Methods 120 cases of CHD from March 201 1 to September 2015 in our hospital were selected as the research subjects.According to the different Gensini scores,they were divided into group A (Gensini score <40 ),group B (Gensini score 40 -60 ),group C (Gensini score 60 -80 ),group D (Gensini score >80 ),with 30 patients in each group.All patients were given atorvastatin treatment,the therapeutic effect of the four groups,the total cholesterol(TC),three glycerol(TG),high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL -C)and hs -CRP levels after treatment were compared.Results After treatment,the lower the Gensini score,the better curative effect,four groups had statistically significant difference in the therapeutic effects(P<0.05),and the total effective rates of group A,B, C and D were 96.7%,86.7%,76.6%,56.7%.After treatment,the TG,TC,HDL-C,LDL-C in four groups were improved,A group improved significantly better than the B group,B group improved better than those of C group,C group improved better than those of D group,the differences were statistically significant(F=6.253,6.258,5.856, 6.259,all P<0.05).After treatment,the levels of hs-CRP in group A,B,C and D were (8.2 ±3.9)mg/L,(11.9 ± 6.9)mg/L,(19.2 ±9.3)mg/L,(24.5 ±11.6)mg/L,the hs-CRP levels of four groups were lower,hs-CRP level after treatment in group A was lower than that of B,C,D groups;and hs-CRP level after treatment in group B was lower than that of group C,D;hs-CRP level of C group after treatment was lower than that of group D.The differ-ences were statistically significant(t=12.651,9.185,4.982,3.030,all P<0.05).Conclusion The level of serum hs-CRP in patients with CHD is higher,and the level of hs-CRP is positively correlated with the Gensini score.

Objective:To explore the effects and problems of on-the-spot surveys to provide reference for promoting the investigation,analysis and evaluation of adverse drug reactions/events.Method:By the retrospective case study, 27 ADR/ADE death reports and their corresponding on-the-spot surveys were analyzed in Jiangsu province in 2008.Result: The death occurred more in the elderly,the ADR drags were more seen in the use of antibiotics,and ADRs occurred highly in the venous injection,and the the completion rate of the scene survey was 44.44%.Conclusion:The on-the-spot survey needs good cooperations from various departments,and the management of drugs should be strengthened.

OBJECTIVE:To establish an UV method for the dermination of main components in rilmazafone hydrochloride tablets. METHODS: The content of rilmazafone was determined by UV spectrophotometry in accordance with Chinese Pharmacopeia (2005 edition). The detective wavelength was set at 254 nm. RESULTS: The linear range of rilmazafone was 2.5～30 ?g?mL-1 (r=0.999 9) and the average recovery was 99.41% (RSD=0.59%,n=10). CONCLUSION: The method is simple, accurate and applicable for the determination of rilmazafone in this preparations.

In recent years , respiratory tract infectious diseases and public health events caused by human adenovirus type 7(HADV-7) had been rising.Especially in Asia, the outbreaks of acute respiratory illness caused by HADV-7, accounting for more than 60%in the world, had brought great influence to the public health.In order to prevent the occurrence of HADV-7 epidemic and improve the diagnostic performance of laboratory , the research progress of HADV biological features and typing , HADV-7 epidemiological characteristics , clinical and differential diagnosis and laboratory examination methodologies were reviewed in this paper.

Aim To investigate the effects of curcumin on expression of HIF-1? in human hepatocellular carcinoma BEL-7402 cells.Methods Different concentrations of 0,2.5,5,10,15 and 20 ?mol?L~(-1) curcumin-treated human hepatocellular carcinoma BEL-7402 cells were cultured under hypoxia for 6 hours.The cell proliferation and cell viability were assayed by WST-8 and the trypan blue dye exclusion method.The expression level of HIF-1? in human hepatocellular carcinoma BEL-7402 cells was checked by RT-PCR and Western Blot;0,10 ?mol?L~(-1) curcumin,10 ?mol?L~(-1) MG-132,and 10 ?mol?L~(-1) curcumin +10 ?mol? L~(-1) MG-132-treated human hepatocellular carcinoma BEL-7402 cells were cultured under hypoxia for 6 hours.The protein expression level of HIF-1? protein in human hepatocellular carcinoma BEL-7402 cells was checked by Western Blot.Results ① No significant difference was found in the cell proliferation rate and cell viability between control and different concentrations of curcumin.② Curcumin can down-regulate the expression of HIF-1? protein with the increasing concentrations.③ the effect of curcumin on expression of HIF-1?mRNA had no significant difference between control and different concentrations.④ MG-132 can reverse the curcumin-mediated decrease of HIF-1? protein.Conclusion Inhibitory effect of curcumin on expression of HIF-1? in human hepatocellular carcinoma BEL-7402 cells was acted through posttranscriptional mechanisms and the proteasome pathway.

[Objective]To evaluate the therapeutic effect of arthroscopic treatment for osteoarthritis of the knee,and explore the factors related to clinical outcome. [Methods]Three hundred and sixty-two patients with knee osteoarthritis were treated by arthroscopic surgery in the 88th hospital from May 2000 to November 2007. Of them,124 patients were followed-up completely. Quantified clinical data were input into computer and analyzed statistically by SPSS 16.0 software. Lysholm score was used to evaluate the therapeutic effect. Lysholm scores before surgery and at follow up were compared with paired t test. Patients were divided into different subgroups according to sex,locking,X -ray grade,arthroscopic grade and surgical methods. Lysholm scores at the final follow up of different subgroups were compared with variance analysis. The factors related to operation outcome were explored by using multiple stepwise linear regression analysis.[Results]One hundred and twenty-four patients were followed up from 7 to 96 months with an average of 39.6 months. Preoperative Lysholm score was (46.10?2.80),the score at follow up was (70.69+2.90),there was significant difference between preoperative score and at follow up (t=9.362,P0.05). The difference was significant between groups whether locking or not (F=10.654,P

A high dose E. Coli endotoxin was infused into unanesthetized sheep withchronic lung lymph fistula. The contents of substance P in plasma, lung lymph, lungparenchyma and also TXB_2 and 6-keto-PGF_1? were measured. The results showed that 120min after infusion substance P increased in plasma and lymph, but its content in paren-chyma decreased at 360 min after infusion. At 30 min after infusion, TXB_2 and 6-keto-PGF_1? levels in plasma showed a marked increase, but their peak values in lung lymphwere shown at 60 min after infusion, and the levels of TXB_2 were much higher than thatof 6--keto--PGF_1?

Objective To explore the features of nerve electrophysiology in Miller-Fisher syndrome (MFS) in children.Methods Thirty-four children with MFS were selected.The examinations of routine motor sensory nerve conduction,H reflexes of soleus muscle and F wave of median and tibial nerevs were performed.At the same time,33 healthy children of the same age were selected as control group and 30 children with typical Guillain-Barre syndrome (GBS) selected as GBS group,which were compared with children with MFS.Results In children with MFS,routine motor nerve conduction velocity and amplitude,and sensory nerve conduction velocity were in normal range.Twelve cases (35.29％) showed a decrease in sensory nerve amplitude potential (SNAP),5 cases (14.71％) showed sural sparing phenomena (the decline degrees of SNAP in median or ulnar nerve greater than in sural nerve),22 cases (64.71％) showed abnormality of H reflex,and 12 cases (35.29％) showed the decline of SNAE Compared with control group,there was no difference in sensory nerve conduction velocity and SNAP in sural nerve in children with MFS (P＜0.05);While there were significant decreases in SNAP in both median nerve and ulnar nerve (P＜0.05).Compared with GBS group,children with MFS had significantly higher sensory conduction amplitude and faster velocity (P＜0.01).Conclusion A decrease of terminal sensory nerve conduction amplitude could be found in children with MFS,but,the SNAP had limited impact on it.H reflex abnormality is the most common electrophysiologic abnormalities in MFS.

BACKGROUND: Unicompartmental knee arthroplasty (UKA) began in the early 1970s, which was once negated due to high postoperative repair rate. The methods for early unicompartment osteoarthritis of the knee include UKA, total knee arthroplasty, high tibial osteotomy and fibular truncation. A large number of retrospective studies and literatures have pointed that UKA is effective for unicompartment osteoarthritis of the knee and holds unique advantages.OBJECTIVE: To review the status and research progress of UKA for anteromedial compartment osteoarthritis of the knee in view of indications, contraindications, operation curve, operation process and main technical points and clinical efficacy.METHODS: The first author retrieved the databases of PubMed and CNKI from March 2006 to September 2016 using the keywords of unicompartmental knee arthroplasty, knee, osteoarthritis, clinical application in English and Chinese,respectively. A total of 95 literatures were searched, and 40 eligible articles were included in accordance with the inclusion criteria.RESULTS AND CONCLUSION: Only unilateral lesion (the degenerative cartilage surface of tibiofemoral joint) is replaced in UKA to treat early unicompartment osteoarthritis of the knee, which exhibits less trauma, rapid recovery, few complications, and normal postoperative proprioception of joint and high patient acceptance. Because of its narrow surgical indications, UKA application has been restricted compared with total knee arthroplasty. Indeed, total knee arthroplasty is matureand, and its long-term curative effect is clear. In contrast, UKA is carried out late in China, has not been popularized, and the long-term clinical efficacy remains to be verified further. But if we can accurately grasp the operation indications, choose appropriate patients, make careful preoperative preparation, and master mature skills, the clinical effect will be satisfactory. With the development of prosthesis, equipment design and operation technology, UKA will be prevailed in the treatment of anteromedial compartment osteoarthritis of the knee.

The Wnt/?-catenin signal transduction pathway is essential for regulating cell proliferation,movement,differentiation and embryo development. Researchers have found that Wnt pathway is an important regulation factor in the early stimulation of the cardiogenesis and contacts closely the several key events during cardiac morphogenesis. The article reviews the recent studies about the relation between cardiac development and Wnt pathway in early embryo.