Objective:To explore the effects and problems of on-the-spot surveys to provide reference for promoting the investigation,analysis and evaluation of adverse drug reactions/events.Method:By the retrospective case study, 27 ADR/ADE death reports and their corresponding on-the-spot surveys were analyzed in Jiangsu province in 2008.Result: The death occurred more in the elderly,the ADR drags were more seen in the use of antibiotics,and ADRs occurred highly in the venous injection,and the the completion rate of the scene survey was 44.44%.Conclusion:The on-the-spot survey needs good cooperations from various departments,and the management of drugs should be strengthened.

Objective To study the effect of atorvastatin on the levels of high sensitive C-reactive protein (hs-CRP)in coronary heart disease(CHD)patients with different Gensini score.Methods 120 cases of CHD from March 201 1 to September 2015 in our hospital were selected as the research subjects.According to the different Gensini scores,they were divided into group A (Gensini score <40 ),group B (Gensini score 40 -60 ),group C (Gensini score 60 -80 ),group D (Gensini score >80 ),with 30 patients in each group.All patients were given atorvastatin treatment,the therapeutic effect of the four groups,the total cholesterol(TC),three glycerol(TG),high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL -C)and hs -CRP levels after treatment were compared.Results After treatment,the lower the Gensini score,the better curative effect,four groups had statistically significant difference in the therapeutic effects(P<0.05),and the total effective rates of group A,B, C and D were 96.7%,86.7%,76.6%,56.7%.After treatment,the TG,TC,HDL-C,LDL-C in four groups were improved,A group improved significantly better than the B group,B group improved better than those of C group,C group improved better than those of D group,the differences were statistically significant(F=6.253,6.258,5.856, 6.259,all P<0.05).After treatment,the levels of hs-CRP in group A,B,C and D were (8.2 ±3.9)mg/L,(11.9 ± 6.9)mg/L,(19.2 ±9.3)mg/L,(24.5 ±11.6)mg/L,the hs-CRP levels of four groups were lower,hs-CRP level after treatment in group A was lower than that of B,C,D groups;and hs-CRP level after treatment in group B was lower than that of group C,D;hs-CRP level of C group after treatment was lower than that of group D.The differ-ences were statistically significant(t=12.651,9.185,4.982,3.030,all P<0.05).Conclusion The level of serum hs-CRP in patients with CHD is higher,and the level of hs-CRP is positively correlated with the Gensini score.

OBJECTIVE:To establish an UV method for the dermination of main components in rilmazafone hydrochloride tablets. METHODS: The content of rilmazafone was determined by UV spectrophotometry in accordance with Chinese Pharmacopeia (2005 edition). The detective wavelength was set at 254 nm. RESULTS: The linear range of rilmazafone was 2.5～30 ?g?mL-1 (r=0.999 9) and the average recovery was 99.41% (RSD=0.59%,n=10). CONCLUSION: The method is simple, accurate and applicable for the determination of rilmazafone in this preparations.

China ; epidemiology ; Endemic Diseases ; prevention & control ; statistics & numerical data ; Humans ; Public Health Practice

OBJECTIVETo study the effect of nadroparin in the migration of breast cancer cells MDA-MB-231 and its action mechanism.

METHODThe MTT test was adopted to observe the effect of different concentrations of naringenin on the growth capacity of breast cancer cells MDA-MB-231. Wound healing and transwell experiment analysis were conducted to detect the effect of naringenin on the migration of breast cancer cells MDA-MB-231. Western blotting was adopted to investigate the effect of naringenin on protein expressions of MDA-MB-231 cell Integrin β3, β1 and matrix metalloproteinase MMP-2 and MMP-9. The computer virtual docking technique was used to evaluate the combining capacity of naringenin and Integrin β3 in vitro.

RESULTNaringenin inhibited the migration of MDA-MB-231 cells in a dose-dependent manner. In wound healing and transwell experiments, with the increase in the concentration of naringenin, the number of migrant MDA-MB-231 cells and the invasion capacity of breast cancer cells decreased. Naringenin could inhibit the protein expression of Integrin β3 in a dose-dependent manner, but with unobvious effect on expression of Integrin β1. Besides, naringenin could significantly inhibit the protein expressions of MMP-2 and MMP-9. The results of the computer virtual docking showed a negative value in the combining capacity between naringenin and Integrin β3, indicating the high affinity between them.

CONCLUSIONNaringenin can inhibit the growth capacity of breast cancer cells MDA-MB-231 and block the migration and invasion of breast cancer cells MDA-MB-231. Its mechanism is to down-regulate MMP-2 and MMP-9 expressions after combining with Integrin β3.

Breast Neoplasms ; drug therapy ; genetics ; metabolism ; pathology ; Cell Line, Tumor ; Cell Movement ; drug effects ; Drugs, Chinese Herbal ; pharmacology ; Female ; Flavanones ; pharmacology ; Gene Expression Regulation, Neoplastic ; drug effects ; Humans ; Integrins ; genetics ; metabolism ; Matrix Metalloproteinase 2 ; genetics ; metabolism ; Matrix Metalloproteinase 9 ; genetics ; metabolism ; Neoplasm Invasiveness

Individual dose monitoring and registry for radiation workers plays a significant role in ionizing radiation protection and occupational health management. Since the nationwide implementation of monitoring from the 1980s, individual dose monitoring for radiation workers in China has been going on nearly 30 years, which is of significance in radiation protection optimization, and occupational health and protection for radiation workers. In order to improve individual dose monitoring and registry, to prevent and control occupational radiation diseases, and to help build "Healthy China" program, this paper presents the evolution of individual dose monitoring and registry in China, discusses the key points and difficulties faced, and proposes suggestions.

Aim To investigate the effects of curcumin on expression of HIF-1? in human hepatocellular carcinoma BEL-7402 cells.Methods Different concentrations of 0,2.5,5,10,15 and 20 ?mol?L~(-1) curcumin-treated human hepatocellular carcinoma BEL-7402 cells were cultured under hypoxia for 6 hours.The cell proliferation and cell viability were assayed by WST-8 and the trypan blue dye exclusion method.The expression level of HIF-1? in human hepatocellular carcinoma BEL-7402 cells was checked by RT-PCR and Western Blot;0,10 ?mol?L~(-1) curcumin,10 ?mol?L~(-1) MG-132,and 10 ?mol?L~(-1) curcumin +10 ?mol? L~(-1) MG-132-treated human hepatocellular carcinoma BEL-7402 cells were cultured under hypoxia for 6 hours.The protein expression level of HIF-1? protein in human hepatocellular carcinoma BEL-7402 cells was checked by Western Blot.Results ① No significant difference was found in the cell proliferation rate and cell viability between control and different concentrations of curcumin.② Curcumin can down-regulate the expression of HIF-1? protein with the increasing concentrations.③ the effect of curcumin on expression of HIF-1?mRNA had no significant difference between control and different concentrations.④ MG-132 can reverse the curcumin-mediated decrease of HIF-1? protein.Conclusion Inhibitory effect of curcumin on expression of HIF-1? in human hepatocellular carcinoma BEL-7402 cells was acted through posttranscriptional mechanisms and the proteasome pathway.

BACKGROUND: Unicompartmental knee arthroplasty (UKA) began in the early 1970s, which was once negated due to high postoperative repair rate. The methods for early unicompartment osteoarthritis of the knee include UKA, total knee arthroplasty, high tibial osteotomy and fibular truncation. A large number of retrospective studies and literatures have pointed that UKA is effective for unicompartment osteoarthritis of the knee and holds unique advantages.OBJECTIVE: To review the status and research progress of UKA for anteromedial compartment osteoarthritis of the knee in view of indications, contraindications, operation curve, operation process and main technical points and clinical efficacy.METHODS: The first author retrieved the databases of PubMed and CNKI from March 2006 to September 2016 using the keywords of unicompartmental knee arthroplasty, knee, osteoarthritis, clinical application in English and Chinese,respectively. A total of 95 literatures were searched, and 40 eligible articles were included in accordance with the inclusion criteria.RESULTS AND CONCLUSION: Only unilateral lesion (the degenerative cartilage surface of tibiofemoral joint) is replaced in UKA to treat early unicompartment osteoarthritis of the knee, which exhibits less trauma, rapid recovery, few complications, and normal postoperative proprioception of joint and high patient acceptance. Because of its narrow surgical indications, UKA application has been restricted compared with total knee arthroplasty. Indeed, total knee arthroplasty is matureand, and its long-term curative effect is clear. In contrast, UKA is carried out late in China, has not been popularized, and the long-term clinical efficacy remains to be verified further. But if we can accurately grasp the operation indications, choose appropriate patients, make careful preoperative preparation, and master mature skills, the clinical effect will be satisfactory. With the development of prosthesis, equipment design and operation technology, UKA will be prevailed in the treatment of anteromedial compartment osteoarthritis of the knee.

Objective To discuss the association between vitamin D level and the severity of diabetic retinopathy and maculopathy in type 2 diabetes mellitus.Methods 657 patients with type 2 diabetic patients were included in our study and classified into four groups according to their severity of diabetic retinopathy:the non-diabetic retinopathy group(NDR group, n=257) ,the background diabetic retinopathy group(BDR group, n=243), the preproliferative diabetic retinopathy group(PPDR group, n=135) and the proliferative diabetic retinopathy group(PDR group, n=22).The patients were divided into maculopathy(n=563) and non-maculopathy(n=94).Characteristics data were recorded and disease duration, body mass index, blood pressure and other lipid makers were measured and compared among groups.Results Among four diabetic retinopathy groups, 25(OH)D was statistically different (P <0.05), however, compared to diabetic maculopathy group, the level of 25(OH)D in non-maculopathy group was not statistically different.Logistic regression showed that 25(OH)D was significantly associated with the severity ofdiabetic retinopathy(P<0.05), while not with diabetic maculopathy.Conclusion The level of 25 ( OH ) D is closely associated with the severity ofdiabetic retinopathy in patients with type 2 diabetes mellitus, and it might be an important predictor for the severity of diabetic retinopathy.

The Wnt/?-catenin signal transduction pathway is essential for regulating cell proliferation,movement,differentiation and embryo development. Researchers have found that Wnt pathway is an important regulation factor in the early stimulation of the cardiogenesis and contacts closely the several key events during cardiac morphogenesis. The article reviews the recent studies about the relation between cardiac development and Wnt pathway in early embryo.