Air ; analysis ; Chromatography, Gas ; methods ; Naphthalenes ; analysis ; Workplace

OBJECTIVE:To explore the approach for the improvement and extension of the function of pharmaceutical services in modern hospitals.METHODS:The development of our pharmacy department in recent years was analyzed,aimed at the actual demands of hospitals and patients and in view of the advantages of our department,the new entry point for the pharmaceutical services was explored.RESULTS & CONCLUSIONS:By improving the workflow of prescription dispensing,the time for patients to have their prescriptions filled can be greatly shortened and is left for pharmacists to collect drug-related information based on the demands of both patients and clinic and provide professional guidance so as to increase the weight of patients in the treatment course,which is in the interest of medication safety,patients and hospital.

Humans ; Purpura, Thrombocytopenic ; diagnosis ; therapy

ObjectiveTo evaluate the change of extracellular matrix (ECM) in diabetic rat lungs.MethodsUsing special staining,immunohistochemical method and imaging analysis,the changes of distribution,area,integral optial density and relative contents of lung ECM in diabetic rats were studied.ResultsThe distributions of elastic fibres,collagenic fibres and reticular fibres increased and roughened.The type Ⅳ collagen strong staining was localized in alveolar septum,the basement membranes of bronchiole and capillary,and distributed in cordal,plexiform.Laminin was strong stained and found in alveolar septum,epithelial cells of alveolar,and the basement membranes of bronchiole and pulmonary vessels in cordal and linear distribution.Image analysis showed the area,integral optical density and relative contents of elastic fibres,collagenic fibres,reticular fibres,type Ⅳ collagen and laminin were larger in diabetic rat lungs than in control groups.ConclusionThese results showed that the changes of ECM in early diabetic rat lungs were abnormal.

Objective To investigate the risk factors of postoperative complications of delayed hemorrhage,perforation and digestive tract stenosis after endoscopic submucosal dissection (ESD).Methods The complete data of 793 patients with digestive tract disease who underwent the endoscopic submucosal dissection in the Department of Digestive Surgery in our hospital from January 2011 to December 2014 were retrospectively analyzed.All of the patients were divided into delayed hemorrhage group (n =67) and nonbleeding group (n =726);perforation group (n =47) and non-perforation group (n =746);and digestive tract stenosis group (n =38) and non-stenosis group (n =755).The clinical basic data,lesion related data,and operation related data were independent risk factor and analyzed by single factor analysis and Logistic multiple factor regression analysis.Results The incidence of delayed bleeding,perforation and stenosis in patients with ESD were 8.45％,5.93％,and 4.79％,respectively.The results of single factor analysis:the risk factors for delayed bleeding were long-term use of anticoagulant drugs,gastric sinus disease,lesion diameter,and lesion excision (P ＜ 0.05).The risk factors for postoperative perforation were the diameter of the lesion and the time of operation (P ＜ 0.05).The risk factors of digestive tract stenosis were the esophageal lesions,the diameter of the lesion,and the depth of the lesion to the intrinsic muscle layer (P ＜ 0.05).The results of multi factor Logistic regression analysis:the risk ranking of risk factors for delayed bleeding was gastric antrum occurrence lesion ＞ lesions graded resection ＞ long-term use of anticoagulants ＞ lesion diameter (≥5 mm).The risk ranking of risk factors for perforation was operation time (≥90 mm) ＞ lesion diameter (≥5 mm).The risk ranking of risk factors for digestive tract stenosis was esophageal lesion ＞ lesion diameter (≥ 5 mm) ＞ lesions depth to the muscularis propria.Conclusions For long-term anticoagulation,gastric antrum and fractional resection lesions of patients should pay attention to delayed bleeding.Patients with long operation time are easy to cause postoperative perforation.For long-term anticoagulation,gastric antrum and fractional resection lesions of patients should pay attention to delayed bleeding.

[Objective]To investigate the pathogenesis of osteoarthritis by increasing stiffness of subchondral bone to simulate subchondral bone sclerosis to induce the osteoarthritis.[Methods]The proportion between polymethylmethacrylate(PMMA),methylmethacrylate(MMA),hydroxyapatite(HA) and distilled water was adjusted to keep the maximum reaction temperature under 40℃.The composite material PMMA/HA was obtained and compressed on a mechanical material machine to determine its ultimate strength and stiffness,which were compared with those of subchondral bone.The subchondral bone of medial tibial plateau was scraped,where the composite material PMMA /HA was implanted into.Rabbits were sacrificed at 3,6,9 and 12 weeks,respectively.The articular cartilages of the medial tibial plateau were harvested for histological observation.Immunohistochemical analysis was adopted to record the expression and distribution of collagen type Ⅱ and MMP-1 in cartilage at protein level,which were compared with the blank and control groups.Transmission electron microscope was used to examine the ultramicroscopic changes of chondrocytes in blank and 6,12-week-postsurgery groups.[Results]With the time going,the experimental groups gradually presented degeneration,and had increased histological Mankin score.TEM demonstrated degenerative changes of chondrocytes.Collagen type Ⅱ expressed mainly in transition zone and upper deep zone,and MMP-1 expressed predominantly in the superficial and upper intermediate layers of cartilage.Both expressions steadily increased with time passing.[Conclusion]The increased stiffness of subchondral bone induced osteoarthritis in rabbits,which suggests that subcondral bone sclerosis may induce cartilage damage,and that subcondral bone sclerosis is one of the initial pathogenesis of osteoarthritis.

Objective: To investigate the effect of up-regulation of miR-181a expression mediated by constructing miR-181a recombinant eukaryotic expression vector pcDNA3.1(+)-miR-181a on cell proliferation, migration and invasion abilities of esophageal carcinoma cell line TE11. Methods: PCR primers were designed and miR-181a precursor sequence was amplified from 95C cell genomic DNA. The product fragments were cloned into pcDNA3.1(+) to construct the recombinant vector pcDNA3.1(+)-miR-181a. The recombinant eukaryotic expression vector pcDNA3.1(+)-miR-181a was transfected into TE11 cells. The expression of miR-181a mRNA was detected by real-time fluorogenic quantitative-PCR (RFQ-PCR). The effects of pcDNA3.1(+)-miR-181a on cell proliferation, migration and invasion abilities of TE11 cells were detected by MTT, wound healing and Boyden chamber methods, respectively. Results: The miR-181a recombinant eukaryotic expression vector pcDNA3.1(+)-miR-181a was successfully established. RFQ-PCR revealed that the mature miR-181a was able to effectively express in TE11 cells transfected with recombinant vector pcDNA3.1(+)-miR-181a (P<0.05). The overexpression of miR-181a could significantly increase the proliferation, migration and invasion abilities of TE11 cells. Conclusion: Overexpression of miR-181a can increase cell proliferation, migration and invasion abilities of esophageal carcinoma TE11 cells. These results may provide experiment references for further research of the role of miR-181a in cancer development and progression. Copyright© 2011 by Tumor.

Objective: Observe the curative effect of basic recipes for removing phlegm and activating blood circulation in experimental model of non-alcoholic fatty liver in rats.Methods: Experimental animal model of non-alcoholic fatty liver in rats were established,then treated with Erchen decoction,TaohongSiwu decoction and the combination decoction.Basic biochemical indexes of rats were tested after treatment.Results: Erchen decoction can reduce the level of liver organ coefficient,cholesterin and aspartate aminotransferase(AST)(P

This study was aimed to observe the effect of Danzhi Jiangtang Capsule ( DJC ) on expression of solu-ble thrombomodulin ( sTM ) and soluble endothelial cell protein C receptor ( sEPCR ) in experimental rats of type 2 diabetes mellitus (T2DM). This study also explored possible mechanisms of Yiqi Yangyin Huoxue method on vascular endothelial tissue and discussed mechanisms of prevention and management of T2DM vascular lesions . A total of 60 male Sprague-Dawley ( SD ) rats were fed for one week and then randomly divided into the nor-mal control group ( group N , n = 12 , rats were fed with routine food ) and diabetes mellitus group ( n = 48 , rats were fed with high-fat diet). After 4 weeks, rats in the diabetes mellitus group were injected with strepto-zotocin ( STZ ) to induce T2DM . According to the blood glucose level , rats were divided into three groups , wh ich were the model group (group M), pioglitazone (group P), and pioglitazone combined with DJC (group D). Group P was treated with pioglitazone (10 mg?kg-1?d-1), group D was given pioglitazone (10 mg?kg-1?d-1) and DJC ( 0 . 47 g?kg-1?d-1 ) . The group N and group M were treated with sodium chloride ( 5 mL?kg -1?d-1 ) . After four-week drug administration , the levels of sTM , sEPCR , PT , APTT and FIB in each group were tested . The results showed that compared with the control group , the levels of sTM , sEPCR and FIB in group M , group P and group D were significantly increased ( P < 0 . 05 ) , and the levels of PT and APTT were obviously reduced ( P < 0 . 05 ) . Compared with group P , the levels of sTM , sEPCR , FIB of group D were significantly reduced ( P< 0 . 01 ) , and the level of APTT was significantly increased ( P < 0 . 05 ) . However , there was no significant dif-ference in the increasing of PT. It was concluded that the Yiqi Yangyin Huoxue method can reduce the levels of sTM and sEPCR , and effectively improve coagulation . The possible mechanisms of protective effect on the vascular endothelium can be from reducing levels of sTM and sEPCR in order to enhance the activity of anti-coagulation and fibrinolysis , regulate the function of blood coagulation to improve hypercoagulable state .

Objective Through analyzing benign and malignant pleural effusion samples by proteomic techniques, finding protein biomarkers to provide help and new clues for effusion differential diagnosis.Methods Two-dimensional electrophoresis(2-DE) and matrix-assisted laser desorption ionization-time of flight-mass spectrometry(MALDI-TOF-MS) were used to search and identify protein biomarkers, enzyme linked immunosorbent assay(ELISA) was to validate the biomarkers.Results By comparing malignant group with benign group, 43 significantly different protein spots(Up or down regulated≥2 times) were found.Including 9 up regulated spots and 34 down regulated spots.And 7 spots were identified(Up or down regulated≥3 times) by MALDI-TOF-MS and validated 2 spots immunoglobulin λ(Igλ) and haptoglobin(Hp) by ELISA.The results showed that Igλ showed no statistical significance between two groups, while Hp showed the statistical significance(P<0.05).The diagnostic sensitivity and specificity of Hp in malignant pleural effusion were 75.00% and 52.38% at diagnostic cut-off point of 389.02 μg/L.Conclusion The application of proteomics technology has a great help with protein biomarkers searching in pleural effusion.HP has a certain value for the differential diagnosis of benign and malignant pleural effusionand and worthy of further study.